AP BIOLOGY CHAPTER 18-REGULATION OF GENE EXPRESSION NOTES AND HOMEWORK NOTES PROKARYOTIC AND PROKARYOTIC GENOME Sources of Variation and Recombination 1|Page EUKARYOTIC GENOME AP BIOLOGY CHAPTER 18-REGULATION OF GENE EXPRESSION NOTES AND HOMEWORK 2|Page AP BIOLOGY CHAPTER 18-REGULATION OF GENE EXPRESSION NOTES AND HOMEWORK OPERONS – 3|Page TRP OPERON AP BIOLOGY CHAPTER 18-REGULATION OF GENE EXPRESSION NOTES AND HOMEWORK OPERONS – 4|Page LAC OPERON AP BIOLOGY CHAPTER 18-REGULATION OF GENE EXPRESSION NOTES AND HOMEWORK EUKARYOTIC GENOME 5|Page AP BIOLOGY CHAPTER 18-REGULATION OF GENE EXPRESSION NOTES AND HOMEWORK HOMEWORK 1. NAME:_____________________ Listed below are descriptions and examples of the different types of recombination in bacteria. Classify each type as: A) transformation B) transduction C) conjugation D) transposons Bacteria assimilate genetic material from surroundings Bacteria take up DNA from environment Gene transfer from one bacterium to another via bacteriophage Direct transfer of genes between two bacterial temporarily joined by sex pili Jumping genes first described by Barbara McClintock Pieces of DNA move from one location to another location in cell’s genome A DNA segment is moved from one location to another DNA is transferred from one bacterium to another by a virus A plasmid is exchanged between two bacteria through a pilus Bacterial strains A and B are growing together in a colony that has been infected with viruses. After a short period of time, a new strain of bacteria is detected that is very similar to strain A but has a few characteristics of B. The conversion of live R strain Streptococcus pneumoniae into S strain when heat-killed S strain is added to R strain E. coli bacteria are induced to take up the pBLU plasmid 2. List and describe the two main strategies used by cells to control metabolism. METHOD OF CONTROL 6|Page DESCRIPTION OF METHOD AP BIOLOGY CHAPTER 18-REGULATION OF GENE EXPRESSION NOTES AND HOMEWORK 3. 4. Match the description/function with the correct term. Regulated cluster of adjacent structural genes with related functions Gene that codes for polypeptide A. Corepressor Transcript of several genes; translated into several polypeptides RNA segment between promoter & structural genes; controls access of RNA polymerase to structural gene Specific protein that binds to operator and blocks transcription Genes that code for repressor or regulators of other genes RNA polymerase binding site C. Operon Molecule that binds to repressor protein; complex then binds to operator H. Structural gene Explain how the trp operon works: a. If tryptophan is absent from the cell. b. If tryptophan is present in the cell 7|Page B. Operator D. Polycistronic mRNA E. Promoter F. Regulatory gene G. Repressor AP BIOLOGY CHAPTER 18-REGULATION OF GENE EXPRESSION NOTES AND HOMEWORK 5. Explain how tryptophan acts as a corepressor. 6. Match these components of the lac operon with their functions. 7. -galactosidase A. cAMP-CAP complex B. lactose C. operator D. promoter E. regulator gene F. repressor G. structural gene H. is inactivated when attached to lactose codes for synthesis of repressor hydrolyzes lactose stimulates gene expression repressor attaches here RNA polymerase attaches here acts as inducer that inactivates repressor codes for an enzyme Listed below are characteristics of repressible and inducible enzymes. Identify each of the following as true of repressible or inducible enzymes. genes are switched off until a specific metabolite inactivates the repressor genes are switched on until a specific metabolite activates the repressor Generally function in anabolic pathways Usually function in catabolic pathways Pathway end product switches off its own production Enzyme synthesis is switched on by the nutrient in used in the pathway 8|Page AP BIOLOGY CHAPTER 18-REGULATION OF GENE EXPRESSION NOTES AND HOMEWORK 8. The events listed below describe how the lac operon functions when lactose is present and glucose is absent. Put the steps in the correct order. Allolactose binds to repressor cAMP accumulates cAMP activates CAP cAMP binds to CAP cAMP/CAP complex binds to CAP site in promoter CAP concentration increases Genes transcribed Repressor inactivated RNA polymerase binds to promoter 9. Explain how the lac operon functions when both lactose and glucose are present in the cell. 10. Match the term with the correct definition or description. A. C. E. G. 30-nm chromatin fiber Heterochromatin Looped domains Nucleosomes B. Euchromatin D. Histone proteins F. Metaphase chromosome Small proteins associate with DNA packing Beads on a string; DNA wound around histone proteins Tightly would coil with 6 nucleosomes per turn; molecules of one type of histone protein pull the nucleosomes into a cylinder with a diameter of 30 nm Loops of 30-nm chromatin fibers; each loop contains 20,000 to 100,000 base pairs 9|Page AP BIOLOGY CHAPTER 18-REGULATION OF GENE EXPRESSION NOTES AND HOMEWORK Folding of the looped domains Chromatin that remains highly condensed during interphase; actively transcribed not Chromatin that is less condensed during interphase; actively transcribed 11. Listed below are the levels of chromatin (DNA) packing in the eukaryotic genome. Put them in the correct order starting with the least condensed. Looped domains Nucleosomes Metaphase chromosome 30-nm chromatin fiber 10 | P a g e