TERRESTRIAL ARTHROPOD COLLECTION PRE-AP BIOLOGY Purpose: Arthropods are the most abundant phylum Arthropoda there is great investigate the diversity of the locally and illustrate this with organisms on Earth. Within the variety. Your job is to arthropods that can be found a small collection. Insects are everywhere!! Insects are often encountered, at least with a little searching, in homes, yards, around building foundations, basements, crawl spaces, flower or vegetable gardens, around lights at night, near streams and lakes, parks, and forests. Dead insects in reasonable condition (for collections) can often be found on windowsills, car grills, roadsides and walking paths. Some insects are very sedentary and are easy to catch with a pair of tweezers. Others fly, some pretty slowly and others (like dragonflies) are FAST! Catching insects takes some practice. Materials Shoebox w/ transparent top Styrofoam (to fit bottom) Fine straight pins 3X5 notecard (for labels & triangles) possibly: jar with lid flashlight handlens cottonball nail polish remover glue net/drop cloth References: Taxonomic key (attached) Taxonomy Guides (reference books/internet resources) such as: http://nature.berkeley.edu/citybugs/studentcorner/MakeABugCollect ion/MakeABugCollection.htm www.Insectidentification.org/insect-key.asp Procedure: In general, you are going to 1) kill the arthropod, 2) pin the arthropod, and 3) identify the arthropod. Below is a summary of some of the easier approaches to these steps. 1. Killing the Arthropod –Glue a cotton ball to the lid of a small jar. Partially dampen the cotton ball with nail polish remover. Put the arthropod in the jar for several hours. Do not allow the liquid to get on the specimen. (insect relaxing chamber) Freezing an insect does not guaranty it will stay dead. 2. Pinning the Arthropod – pinning varies with the type of arthropod. In general, you want to make sure that you do not damage the organism and that it is securely attached to your display board. Some guidelines to follow are: - pins usually do NOT go in the middle of the body (see diagrams) butterflies and moths should have their wings spread out flat specimens should be suspended on the pin, a little below the head hold the specimen between the thumb and forefinger of one hand and insert the pin with the other for very tiny insects, use elongated triangles of cardboard and VERY SMALL amounts of glue to attach the specimen to the point of the cardboard, then pin the cardboard (see illustration) 3. Identifying the Arthropod – use a reference manual/guide to determine the classification of the organism to the level of Order for all acceptable organisms except millipedes and centipedes. You are only required to go to the level of Class for millipedes and centipedes. The identification will go on a card placed below the specimen. Requirements of Collection: Your collection must contain a minimum of 20 specimens, each correctly preserved, pinned, and identified and displayed in alphabetical order according to CLASS then ORDER. You should collect from a variety of classes/orders. All specimens collected must be typically found in TEXAS. Use caution when collecting venomous organisms. Your collection MUST be turned in on _September 27__________. LATE PROJECTS WILL NOT be accepted! Attached are examples of guides to the major divisions of terrestrial arthropods. There are other versions, especially in the orders of insects. My advice to you is “be consistent”. Try to stick with ONE classification system, a copy of your chief reference should be noted. Be sure you can verify your classification for me if I have a question! You may collect more than one Arthropod from each of the following classes or orders but not multiple of the same bug. For instance, you may have a grasshopper and a cricket but not two grasshoppers. ****NO TARANTULAS****** You may NOT buy or purchase bugs. Please see Rubric for specific details on identification labels and master list. The final product will be a decorated box with a clear lid, a master list attached to outside of box, 20 insects in alphabetical order, correctly pinned and identified on a id card under the bug. ARTHROPOD CLASSIFICATION Class: Diplopoda – millipedes Chilopoda - centipedes Insecta – insects Order: Orthoptera – grasshoppers, crickets, mantids, walkingsticks Ephemeroptera – mayflies Thysanura- silverfish, firebrats Mantodea- mantids Phasmida- Walking sticks Diptera – flies and mosquitoes Lepidoptera – butterflies and moths Trichoptera – caddis flies Hymenoptera – ants, wasps, and bees Neuroptera – dobson flies, lacewings, and ant lions Mecoptera - scorpionflies Odonata – dragonflies and damselflies Homoptera – cicadas, hoppers, aphids Isoptera - termites Plecoptera - stoneflies Hemiptera – true bugs Anoplura – human lice Dermaptera - earwigs Coleoptera - beetles Siphonaptera – fleas Blatteria- cockroaches Malacostraca Order: Isopoda (only) – pill bugs (aka rolly-pollies) Arachnida Order: Scorpiones – scorpions Acari – mites Araneae – spiders GRADING RUBRIC PROJECT 1: TERRESTRIAL ARTHROPOD COLLECTION PRE-AP BIOLOGY STUDENT NAME __________________________________ PERIOD _______ Display (25 points) Box appropriately decorated w/see-thru lid Name, class period, and due date on box Insects neatly and appropriately/alpha order by class/order Triangular cardboards cleanly cut and uniform Identification labels typed in 6 to 8 point font or neatly written Master list of specimens (10 points) _______/10 _______/5 _______/5 _______/3 _______/2 _______/10 On 3 X 5 notecard Glued neatly to outside of box Teacher Discretion (5 points) _______/5 Comments ___________________________________________________ _____________________________________________________________ _____________________________________________________________ COLLECTION – 3 points per specimen (60) Collected 20 organisms (1 point/specimen) Identification pinned under each insect (1 points/specimen) *Classification: Phylum, Subphylum, Class, Order *Date collected *County, city & state where collected *Specimens numbered to match master list Technique (1 point/specimen) *Specimens properly pinned *Pins straight up and down *Small or soft-bodied organisms on a triangular cardboard in a vial *Wings spread (where required) *No missing/decaying body parts Lowest Classification 1._______________________ 2._______________________ 3._______________________ 4._______________________ 5._______________________ 6._______________________ 7._______________________ 8._______________________ 9._______________________ 10._______________________ 11._______________________ 12._______________________ 13._______________________ 14._______________________ 15._______________________ 16._______________________ 17._______________________ 18._______________________ 19._______________________ 20._______________________ Collection Total ___________/60 Total Points _____________/100 “Bug” ID Technique 1. KEY TO INSECT ORDERS A. Insects with wings………………………………………….……………………………….…………………………………………..……Go to 2 B. Insects without wings or only rudimentary wings……………………………………………………..…………………….…………Go to 16 2. A. Insects with only one pair of thin, usually transparent wings; second pair replaced with short, pinlike structures (flies, mosquitoes)Order Diptera B. Insects with two pairs of wings………………………………………………………………………………….…………………….….Go to 3 3. A. Two pair of wings not alike in structure (not equal in thickness or transparency) (beetles, true bugs, grasshoppers)…………..Go to 4 B. Two pairs of wings of similar structure (bees, butterflies, dragonflies)………………..…………………………………..…………Go to 7 4. A. First pair of wings horny and meeting in a straight line down the back (beetles, earwigs)………………….…………………….…Go to 5 B. First pair of wings not as in 4A………………………………………………………………….………….…………………………….Go to 6 5. A. Tip of abdomen with a prominent pair of pincers (earwigs)……………………………..…………………………………………….……Order Dermaptera B. Tip of abdomen without pincers (beetles)…………………………………………….…………………………………………………...Order Coleoptera 6. A. Front wings leathery at base, membranous and overlapping at tip; mouthparts for sucking (true bugs)…………….…………..…Order Hemipterea B. Front wings leathery with veins; hind wings folded lengthwise; mouthparts for chewing (crickets, katydids, grasshoppers).….Order Orthoptera 7. A. Wings wholly or for the most part covered with scales; mouthparts formed for siphoning (moths, butterflies)……….……………Order Lepidoptera B. Wings transparent or thinly clothed with hairs (bees, mayflies, dragonflies)………………………………………………………....Go to 8 8. A. Mouthparts for sucking attached to hind part of lower surface of head; wings when at rest are held like the halves of a roof (cicadas, aphids, leafhoppers, treehoppers)…………………………………………………………………….……………………….Order Homoptera B. Mouthparts not as in 8A………………………………………………………………………………………………………….…………Go to 9 9. A. Body usually brown and slender; insect mothlike with slim antennae; no mouthparts evident except for a pair of slender palpi; wings frequently hairy, usually broadest beyond the middle; wings held like halves of a roof over abdomen (caddisflies)…...Order Trichoptera B. Body and other characteristics not as in 9A…………………………………………………………………………….……………….Go to 10 10. A. Wings with few or no cross veins (bees, thrips)………………………………………………………………………….…………….…Go to 11 B. Wings with many cross veins (dragonflies, lacewings)……………………………………..…………………………….……………Go to 12 11. A. Front wings the larger pair; hind wings frequently hooked to front wings; mouthparts for lapping, chewing, or sucking (bees, wasps)………………………………………………………………………………………………………………………………Order Hymenoptera B. Front wings the same size; wings very narrow; bristly hairs at margin (thrips)…………………………………………………….Order Thysanoptera 12. A. Front pair of wings much larger than hind pair; wings held vertically above body; long, fragile-jointed tails behind (mayflies)Order Ephemeroptera B. Front pair of wings and other characteristics not as in 12 A…………………………….………………………………………..…Go to 13 13. A. Antennae short and inconspicuous; long slender insects with long narrow wings (damselflies, dragonflies)…………………..Order Odonata B. Antennae longer and conspicuous……………………………………………………………………………………………………..Go to 14 14. A. Abdomen usually with two short appendages; back wings much broader than front wings folded lengthwise (stoneflies)………Order Plecoptera B. Abdomen and characteristics not as in 14A………………………………….………………………………………………..………..Go to 15 15. A. Wings equal in size with many distinct veins; tarsi five-jointed (antlions, lacewings)…………………………………………………Order Neuroptera B. Wings equal in size with indistinct veins; tarsi four-jointed; thorax in front of wings very short (termites)………………………..Order Isoptera 16. A. Insects narrow-waisted, antlike (ants)……………………………………………………………………………………………….……Order Hymenoptera B. Insect not narrow-waisted but still antlike……………………………………………………………………………………………….Go to 17 17. A. Bodies antlike but with wide waists, not flattened, light colored (termites)……………………………………………………………Order Isoptera B. Bodies not as in 17A………………………………………………………………………………………………………………..…….Go to 18 18. A. Insects small and plump, soft-bodies with small heads, two short tubes extending from back of abdomen; sucking mouthparts (aphids)………………………………………………………………………………………………………………………………………..Order Homoptera B. Insects not as in 18A…………………………………………………………………………………………………………….…………...Go to 19 19. A. Body of insect small and narrow, flattened on the side; sucking mouthparts hind legs for jumping;five tarsal segments (fleas)Order Siphonaptera B. Body of insect not as in 19A…………………………………………………………….…………………………………………….…Go to 20 20. A. Body covered thickly with scales; mouthparts for sucking are absent (butterflies, moths)…………………………………………Order Lepidoptera B. Body of insect not as in 20A…………………………………………………………………………………………………………….Go to 21 21. A. Insect very delicate with chewing mouthparts and long, jointed, threadlike tails and antennae (bristletails, firebrats,silverfish)…Order Thysanura B. Insect not as in 21A………………………………………………………………………………………………………….………………Go to 22 22. A. Insect delicate with chewing mouthparts; abdomen of six segments; underside of abdomen frequently has a long, usually double appendage used for leaping (springtails)………………………………………………………………………………..Order Collembola B. Insect not as in 22A………………………………………………………………………………………………………….………………Go to 23 23. A. Mouthparts for chewing……………………………………………………………………………………………………………………..…Go to 24 B. Mouthparts for sucking………………………………………………………………………………………………………………………Go to 25 24. A. Antennae threadlike; face directed forward and downward (crickets, roaches, grasshoppers, walkingsticks)……………………..Order Orthoptera B. Antennae beadlike, clublike or comblike, without prominent forceps at tip of abdomen (beetles)…………………………………Order Coleoptera 25. A. Insect apparently legless, frequently covered with a waxy scale; insect usually tightly attached to plant leaves or stems (scale insects)………………………………………………………………………………………………………………………………………………Order Homoptera B. Insect with well developed legs with a sucking beak that arises at front of head and held between legs (true bugs)…………….Order Hemiptera