Exam 2 Practice Exam Week 8.2 Supplemental Instruction Iowa State University Leader: Natalie Course: BIOL 212 Date: 3/4/14 Chapter: 4-13 (no 9 and 10) 1. Which is not true regarding the Na+/K+ pump? a. 3 Na+ exported for 2 K+ imported b. Antiporter system c. Creates a electrochemical gradient d. Both move down their gradient 2. Which is true regarding protein secretion? a. Secreted proteins move from the ER to the Golgi to outside the cell. b. Secreted proteins are inserted into the rough ER to be secreted from the cell. c. Secreted proteins travel from the Golgi to the plasma membrane in vesicles. d. All of the above 3. Which is false regarding emerging polypeptides? a. They can remain in the cytosol b. Proteins destined from the nucleus undergo co-translational sorting c. Proteins destined for the vacuole undergo co-translational sorting d. They can undergo post-translational sorting 4. Reaction A has a ΔG = -7.3 kcal/mole. Reaction B has a ΔG = 3.3 kcal/mole. Which of the following is true? a. Reaction A is endergonic and spontaneous. b. Reaction B exergonic and can take place on its own. c. Reaction B is endergonic and will take place at a slower rate than a Reaction A d. Reaction B will take place if it is coupled to Reaction A 5. Molecule A and B bind to different sites on an enzyme and do not have similar conformations. Which of the following is false? a. This is an example of a noncompetitive inhibitor b. This is an example of a competitive inhibitor c. The Km will be unchanged d. The Vmax will decrease 6. What is true about this reaction: CO2 + H2O + light energy → C6H12O6 + O2 + H2O a. CO2 is oxidized, H20 is unchanged b. H2O is oxidized, CO2 is unchanged c. The left side is oxidized, the right side is reduced d. H2O is oxidized, CO2 is reduced 7. Which of these is incorrectly matched to the structure? a. Calvin cycle, stroma b. Light Reactions, thylakoid c. Citric Acid Cycle, cytosol d. Glycolysis, cytosol 8. How many bands of DNA would you expect to see in a cesium gradient after 3 generations of the mechanism of DNA replication was conservative? a. 1 band that is half heavy b. 2 bands, one is heavy and the other half heavy c. 3 bands, one is heavy, one is half-heavy, and one is light d. 2 bands, one is heavy and one is light 9. What is the function of topoisomerase during DNA replication? a. It separates the two strands of the double helix. b. It relieves the overwinding of DNA ahead of the replication fork due to DNA strand separation. c. It synthesizes the RNA primers used to initiate DNA replication. d. It joins the Okazaki fragments together. 10. Which of these is false about translation? a. During translation, RNA sequences code for polypeptide sequences. b. There are three sites on the ribosome: EPA c. The peptidyl tRNA moves from A to P during Elongation d. The ribosomal subunits and mRNA attach in elongation