AEC MONITORING SHEET TEMPLATES Instructions All sections in blue must be completed prior to submission of a new project application to the AEC. Monitoring frequency must be no less than twice per week. Monitoring sheet: general This sheet is for use during periods when the wellbeing of the animals is not likely to be diminished e.g. during the acclimatisation period, or after an animal has recovered from a particular procedure. This sheet must be used in ALL projects. Monitoring groups o “stock” can be selected to monitor multiple cages on a single sheet. This can only be used for stock animals i.e. only for animals that are not under experiment. o “cage” should be selected when you are monitoring 1 cage of animals on a single sheet. o “individual” should be selected when you are monitoring 1 animal on a single sheet. The monitoring criteria listed in ‘Physical exam’ are mandatory for ALL projects. You should select the applicable monitoring criteria listed in ‘Other monitoring criteria’. Monitoring sheet: acute experiment/anaesthesia This sheet is for use during periods when the wellbeing of an animal may potentially be diminished e.g. diabetic animals, administration of drugs or other treatments, post-surgery. Monitoring groups o “cage” should be selected when you are monitoring 1 cage of animals on a single sheet. o “individual” should be selected when you are monitoring 1 animal on a single sheet. Monitoring criteria examples o General: Activity level, body condition, coat/skin quality, eyes and nose, gait, hydration, posture, social behaviour. o Specific: Blood glucose, blood pressure, tumour size, surgical wound, injection site, temperature, weight, respiratory rate, heart rate, withdrawal reflex. Monitoring sheet: incident record This sheet is for use when an abnormality is found with an animal. Notes on observations, progress of animal’s condition, treatments given and plan must be kept on this sheet. Monitoring sheet: anaesthetic/surgical This sheet is for use during anaesthesia +/- surgery. Each sheet may only be used to monitor the anaesthetic for an individual animal. Monitoring Sheet: General CI name: ENTER CHIEF INVESTIGATOR’S NAME HERE Project #: Room #: Strain: Emergency contact #s: ENTER EMERGENCY CONTACT DETAILS FOR INVESTIGATORS ON PROJECT HERE Cage #s: Animal ID #s: Stock Cage Individual Other (specify) Frequency of monitoring: Daily 3 x weekly 2 x weekly Other (specify) Monitoring group : Physical exam: Other monitoring criteria: Activity level Hydration Blood glucose ☐ Surgical wound ☐ Body condition Posture Weight ☐ Injection site ☐ Coat/skin quality Respiration Other (specify) ☐ Tumour size ☐ Eyes and nose Social behaviour Gait Specific euthanasia criteria (humane endpoints): ENTER HUMANE ENDPOINTS HERE E.G. WEIGHT LOSS > X%, ULCERATION OF SKIN, HUNCHED POSTURE, DEHYDRATION. Description of current project/procedure: Date Initials Physical exam Other monitoring criteria Notes (include details on any experimental procedures performed, diseased/sick animals, singly-housed animals, treatments administered, culled animals) Page ___________ Monitoring Sheet: Acute Experiment CI name: ENTER CHIEF INVESTIGATOR’S NAME HERE Project #: Room #: Strain: Emergency contact #s: ENTER EMERGENCY CONTACT DETAILS FOR INVESTIGATORS ON PROJECT HERE Cage #: Animal ID #s: Cage Individual Other (specify) Frequency of monitoring: Twice daily Daily 3 x weekly Monitoring group: Other (specify) Specific euthanasia criteria (humane endpoints): ENTER HUMANE ENDPOINTS HERE E.G. WEIGHT LOSS > X%, ULCERATION OF SKIN, HUNCHED POSTURE, DEHYDRATION. NOTE: SPECIFIC DETAILS OF ANY ABNORMAL FINDINGS MUST BE RECORDED ON THE INCIDENT RECORD. Date or time Monitoring criteria ENTER MONITORING CRITERIA HERE ENTER MONITORING CRITERIA HERE ENTER MONITORING CRITERIA HERE ENTER MONITORING CRITERIA HERE ENTER MONITORING CRITERIA HERE ENTER MONITORING CRITERIA HERE ENTER MONITORING CRITERIA HERE ENTER MONITORING CRITERIA HERE ENTER MONITORING CRITERIA HERE Investigator’s name Comments/notes Page ___________ Monitoring Sheet: Incident Record Animal ID #: Date ___________________________ Details of abnormal finding and action taken Page ___________ Investigator’s name Monitoring Sheet: Anaesthetic/Surgical Date: _________________ Anaesthetist/Surgeon:_________________________ CI name: ENTER CHIEF INVESTIGATOR’S NAME HERE Project #: Animal ID #: Strain: Pre-anaesthetic exam Weight: g Resting respiratory rate: Coat condition: Hydration status: Behaviour/mentation: Capillary refill time: Other comments: Anaesthetic Start time: Mins End time: 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 Time Iso % Oxygen L/min Withdrawal reflex Resp rate (breaths/min) Skin colour Capillary refill time (sec) Temp (°C) Medications Key events ENTER OTHER MONITORING CRITERIA HERE Normative data: Rats: resp rate 85 breaths/min , CRT<1 sec, temp 37.5°C, heart rate 300-500 beats/min, systolic blood pressure 166 mm Hg. Mice: resp rate 163 breaths/min, CRT<1sec, temp 37-37.2°C, heart rate 310-840 beats/min, systolic blood pressure 133- 160 mm Hg. Surgery notes: Medications Key (a) Drug Dose (mg and ml) Route (b) (c) (d) (e) (f) Page ___________ Comments 95 100 105