Supporting Information
Table S1. Primers designed for real-time RT-PCR of selected protein spots.
Table S2. Effect of 100 µM Cr on SPAD value, maximum photochemical efficiency (Fv/Fm), and
photosynthetic parameters in tobacco leaves of Guiyan 1 and Yunyan 2 after 5 days of Cr
exposure.
Fig. S1 Transmission electron micrographs of chloroplasts of Guiyan 1 (upper) and Yunyan 2
(below) cultured in basic nutrition (A, C) and 100 µM Cr (B, D), respectively. Bar= 0.2µm.
Labels: CW, cell wall; GL, granum lamellae; Os, osmiophilicplastolobuli; SG, starch grain;
SL, stroma lamellae. Figure is representative from three different experiments
Fig. S2 Venn diagram illustrating the abundance of Cr stress-responsive proteins in leaves of
Guiyan 1 (G) and Yunyan 2 (Y). The numbers of protein spots with increased or decreased
abundance are shown in the different segments. As to protein spots altered by 100 µM Cr
stress, the abundance of 86 spots (100 µM Cr vs control) and 107 spots wasincreased in
Guiyan 1 and Yunyan 2, respectively; while of 74, and 77 spots decreased. The abundance of
74 spots increased in both of Yunyan 2 and Guiyan 1 under 100 µM Cr stress, respectively,
and that of 34 decreased.
Fig. S3 Relative expression level of genes of selected proteins under 100 µM Cr stress for 5 days,
compared to their respective controls in two tobacco genotypes. MPP, mitochondrial processing
peptidase-like; DHN, dehydrin; RBP, nuclear-localized RNA binding protein; SOD [Fe], Superoxide
dismutase [Fe].
Table S1 Primers designed for real-time RT-PCR of selected protein spots
Gene
Primer sequence
NtMPP
Forward:CTCAATTCGCTGTTGCATTT
Reverse:TGTTGCCATGACCATCAAA
NtDHN
Forward:GTGAGGACACGGCTGTACC
Reverse:CGCCACTTCCTCTGTCTTCT
NtRBP
Forward:GAAGCTGGACAGGAAGAAGC
Reverse:GGGTCATCTCCTTCTCCTCA
NtSOD [Fe]
Forward:AAAGGCAAATTTGGGAGATG
Reverse:ATAAGCCATCGAAAGGGTTG
β-actin
Forward: TTGACGGAAAGAGGTTAT
Reverse: GTTGGAAGGTGCTGAGAG
Table S2. Effect of 100 µM Cr on SPAD value, maximum photochemical efficiency (Fv/Fm), and
photosynthetic parameters in tobacco leaves of Guiyan 1 and Yunyan 2 after 5 days of Cr exposure.
Treatment
SPAD
value
Fv/Fm
Pn
Tr
Gs
(µmol
(mmol H2O m- (mmol H2O·m2 -1
2 -1
CO2·m−2·s−1)
·s )
·s )
Ci
(µl CO2·L-1)
Guiyan 1
42.5 a
0.799 a 17.12 a
4.32 a
0.312 a
245.54 a
32.07 b
0.661 b 10.73 b
2.88 b
0.169 b
166.67 b
(24.5)
(17.3)
(37.3)
(33.3)
(45.8)
(32.1)
Yunyan 2
Control
42.20 a
0.778 a 17.69 a
4.17 a
0.286 a
240.31 a
Cr
24.8 c
0.544 c 7.26 c
1.45 c
0.112 c
125.36 c
(41.2)
(30.1)
( 59.0)
(65.2)
(60.8)
(47.8)
Different letters indicate significant differences (P<0.05) among each treatment within two genotypes.
Values in parenthesis are the reduced percentage over the control. Pn, net photosynthetic rate; Tr,
transpiration rate; Gs, stomatal conductance; Ci, intercellular CO2 concentration.
Control
Cr
Fig. S1 Transmission electron micrographs of chloroplasts of Guiyan 1 (upper) and Yunyan 2 (below) cultured
in basic nutrition (A, C) and 100 µM Cr (B, D), respectively. Bar= 0.2µm. Labels: CW, cell wall; GL, granum
lamellae; Os, osmiophilicplastolobuli; SG, starch grain; SL, stroma lamellae. Figure is representative from three
different experiments.
G-down
Y-down
Y-up
95
12
34
43
28
Y-constant
239
G-constant
Y-up
21
1
86
Y-down
76
192
Y-constant
75
G-up
Y-up
74
4
33
Y-down
73
8
Y-constant
259
Fig. S2 Venn diagram illustrating the abundance of Cr stress-responsive proteins in leaves of Guiyan 1 (G) and
Yunyan 2 (Y). The numbers of protein spots with increased- or decreased abundance are shown in the different
segments. As to protein spots altered by 100 µM Cr stress, the abundance of 86 spots (100 µM Cr vs control)
and 107 spots wasincreased in Guiyan 1 and Yunyan 2, respectively; while of 74, and 77 spots decreased. The
abundance of 74 spots increased in both of Yunyan 2 and Guiyan 1 under 100 µM Cr stress, respectively, and
that of 34 decreased.
Fig. S3 Relative expression level of genes of selected proteins under 100 µM Cr stress for 5 days, compared to
their respective controls in two tobacco genotypesof Guiyan 1 (G) and Yunyan 2 (Y). MPP, mitochondrial
processing peptidase-like; DHN, dehydrin; RBP, nuclear-localized RNA binding protein; SOD [Fe], Superoxide
dismutase [Fe].