Biology 12 Cell Structure and Function - Chapter 4 Name _________________ How and why does our body develop the way it does? DNA expert J. Craig Venter says, "It's just like solving a jigsaw puzzle, only the jigsaw puzzle in our case has 27 million pieces [and] it came in a very big box and there was no picture on the cover." Vocabulary: cell membrane, cell wall, cellular respiration, chloroplast, chromatin, chromosome, cristae, cytoplasm, cytoskeleton, Golgi bodies, lysosome, matrix, mitochondria, nuclear envelope, nuclear pore, nucleolus, nucleus, organelle, polysome, ribosome, rough endoplasmic reticulum, smooth endoplasmic reticulum, vacuole, vesicle B1 Analyse the functional inter-relationships of cell structures B1.1 describe the following cell structures and their functions: – cell membrane – cell wall – chloroplast – cytoskeleton – cytoplasm – Golgi bodies – lysosomes – mitochondria – including cristae and matrix – nucleus – including nuclear pore, nucleolus, chromatin, nuclear envelope, and chromosomes – ribosomes (polysomes) – smooth and rough endoplasmic reticulum – vacuoles – vesicles B1.2 state the balanced chemical equation for cellular respiration B1.3 describe how the following organelles function to compartmentalize the cell and move materials through it: – rough and smooth endoplasmic reticulum – vesicles – Golgi bodies – cell membrane B1.4 identify cell structures depicted in diagrams and electron micrographs Note: helpful website: http://www.phschool.com/science/biology_place/biocoach/index.html Biology 12: Cell structure and function Page 1 In this unit, we learn how the chemicals that you studied in biochemistry work together within the cell – the smallest unit of life. Biology 12: Cell structure and function Page 2 Every living thing is made of cells. View the following Discovery video: http://media.pearsoncmg.com/bc/bc_campbell_essentials_3/discvids/_html/index.htm?info_text=cc6_c ells 1. When you look at cells under the light microscope, what can you see? Sketch a moss cell or onion (plant) and a human cheek cell (animal) as seen under high power and label the visible structures. You can see some great plant cells on page 51 of your text. You can also see some cool light microscope pictures of animal cells at the following websites: http://www.microscopyu.com/moviegallery/livecellimaging/3t3/index.html http://www.microscopyu.com/moviegallery/livecellimaging/cv1/index.html We’ll do this together in class… Methylene blue stain works well for animals and plant cells do not requires a stain. Animal Cell _____________________________________________________________________ Plant cell 1. Biology 12: Cell structure and function Page 3 2. Use of the electron microscope allows us to see much greater detail in cell structure. Examine at the various electron micrographs provided. http://www.bu.edu/histology/m/t_electr.htm Use this website to draw and label any three cellular structures that you find interesting. Your diagrams must be different to your table partner. Biology 12: Cell structure and function Page 4 By studying many electron micrographs, we are able to see that a typical animal cell contains many small structures that are collectively called ___________________________________. Many of these organelles are bounded by ____________________, which separate the contents of the organelles from other parts of the cell. 3. Use your text to label the following diagram of a typical animal cell, and underline all organelles that are bounded by membranes. View the BioFlix on Animal Cells: Biology 12: Cell structure and function Page 5 4. Use your text to label the following diagram of a typical plant cell, underlining all organelles that are bounded by a membrane. View the BioFlix on Plant Cells: 5. List structures found in plant cells that are not found in animal cells: __________________________________________________________________________________ __________________________________________________________________________________ Biology 12: Cell structure and function Page 6 6. The entire region between the nucleus and the plasma membrane is called the ________________________________________________. The Nucleus (see Sec 4.6 p 58) 7. What are the main functions of the nucleus? ____________________________________________________________________________ ____________________________________________________________________________ 8. What is the function of the nucleolus? ____________________________________________________________________________ 9. Be able to identify each of the following components of the nucleus: chromatin, nuclear envelope, nuclear pores, nucleolus Biology 12: Cell structure and function Page 7 10. What is the difference between chromatin and chromosomes? Use this website visual t o differentiate between chromatin and chromosomes: http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopyuk.org.uk/mag/artaug99/mitosis.html _______________________________________________________________________________ _______________________________________________________________________________ 11. What enters the nucleus through the nuclear pores? ___protein and nucleotides___ What exits the nucleus through the nuclear pores? ___messenger RNA___ 12. How many membranes make up the nuclear envelope? Two = double membrane At some points, it can be seen that the nuclear envelope is joined to the __endoplasmic reticulum _. 13. What is the role of ribosomes in the cell? __to receive the message from mRNA to synthesise a specific protein. __ How do attached ribosomes differ from free ribosomes? Attached ribosomes are attached to the membrane of the rough E R. Free ribosomes are unattached and free in the cytoplasm. _ Why are some ribosomes attached and some ribosomes free? Attached ribosomes produce proteins to be packaged into transport vesicles. Free ribosomes produce proteins to be used in the cell cytoplasm. What are polysomes (polyribosome)? (maybe check internet?) Polysomes are made of many ribosomes reading the same mRNA molecule at the same time and producing many of the same polypeptide as a result. Label this diagram of a polysome: Biology 12: Cell structure and function Page 8 The Endomembrane System 14. What is the endomembrane system and what are its main components? (Sec 4.8 p 59) The entire interconnected membrane system of the cell. It includes cell membrane, endoplasmic reticulum, nuclear membrane, membranes around organelles such as Golgi apparatus. Describe three main functions of the smooth endoplasmic reticulum: (Sec 4.9 p 60) 1. Synthesis of phospholipids for cell membrane use. 2. Synthesis of steroids such as testosterone in the testes and estrogen in the ovaries. These organs are rich in S.E.R. 3. It detoxifies the blood in the liver by processing drugs and other harmful chemicals. As cells are exposed to drugs and alcohol the amount of S.E.R. and detoxifying enzymes increases. 4. Storage of Calcium ions. These are necessary for muscle contraction and neural transmission. Biology 12: Cell structure and function Page 9 15. What are two main functions of the rough endoplasmic reticulum? (Sec 4.9 p 60) ____________________________________________________________________________ ____________________________________________________________________________ ____________________________________________________________________________ Describe the steps shown in the following diagram of the synthesis of a secretory protein: Do activity 4.13 ________________________ ________________________ ________________________ ________________________ ________________________ ________________________ ________________________ ________________________ ________________________ ____________________________________________________________________________ ____________________________________________________________________________ ____________________________________________________________________________ What is the role of the transport vesicle in this process? _______________________________ ____________________________________________________________________________ Name two different secretory proteins that would be manufactured in this manner: Collagen, insulin and other protein hormones, digestive enzymes, like lactase, structural proteins such as keratin. _____________________________________________ Biology 12: Cell structure and function Page 10 16. What are some functions of the Golgi apparatus. (Sec 4.10 p 61) http://www.johnkyrk.com/golgiAlone.html G o l g i r e Receives and modifies molecules manufactured in the ER. These may arrive in the form of protein or lipid filled vesicles. These molecules move through the Golgi from the inner to the outer face of the apparatus. Glycoproteins may be repackaged and sent to the cell membrane for incorporation and phosphates may be added to molecules in the Golgi. How does the Golgi apparatus work with the endoplasmic reticulum? It receives vesicles of molecules from the ER and refines them. What is the role of the transport vesicle? _To transport molecules to different parts of the cell or to the plasma membrane for export or to take phospholipids to the cell membrane for incorporation. Biology 12: Cell structure and function Page 11 17. What is a lysosome ? (Sec 4.11 p 62) _a small membrane bound organelle produced in the Golgi apparatus. Contains lysozymes (enzymes which destroy old organelles or cells). ______ List some functions of the lysosome: 1. Destroy old cells or organelles (recycling), viruses and bacteria 2. Digest food particles by fusing with food vacuoles. Label this diagram illustrating the formation of a food vacuole and the digestion of its contents by a lysosome: page 62 Name one type of cell in your body that would contain lots of lysosomes. Explain. Muscle cells where glycogen needs to be broken down into glucose. Also liver cells , white blood cells and kidney cells. Label this diagram illustrating the use of lysosomes to digest damaged organelles: Biology 12: Cell structure and function Page 12 What is a vacuole? (Sec 4.13 p 62) ___ Membrane bound large storage vesicle ___________________________________________________________________________ What is the function of the central vacuole of a plant cell? Stores water and provides support for the cell. Label the central vacuole on this diagram of a plant cell: You can see the contractile vacuole of a Paramecium at this website: http://02b5260.netsolhost.com/Videos/transport/para_osmotic.wmv How is the contractile vacuole of a Paramecium important to its survival? __________________________________________________________________________________ Label the contractile vacuoles on this diagram of a paramecium: Biology 12: Cell structure and function Page 13 Summary: The endomembrane system is an interconnected system of membranes that works together in synthesis, transport, storage and secretion. Cell organelles and their function 3d and animation http://www.youtube.com/watch?v=LP7xAr2FDFU&feature=related Label the following diagram to illustrate the various parts of the endomembrane system: View: The İnner Life Of The Cell Harvard University HQ You tube Biology 12: Cell structure and function Page 14 Energy Converting Organelles See Sec 4.14 p 63 and 4.15 p 64 See Activity: Build a Chloroplast and a Mitochondrion (4.13) What are the names of the two organelles that are ‘energy transformers’? __________________________________________________________________________________ Mitochondria are the site of the process called __ cellular respiration _. nWrite a balanced equation for this process in words and in formulae: __ Oxygen and glucose produce carbon dioxide and water and energy. One glucose molecule will produce 38 ATPs. Powering the Cell: Mitochondria http://www.youtube.com/watch?v=RrS2uROUjK4&feature=related Label the following diagram and electon micrograph of a mitochondrion: During cellular respiration, mitochondria produce molecules of _______________, which are used in the cell as an energy molecule for cell work. Biology 12: Cell structure and function Page 15 Chloroplasts are the site of the process called ________________________________. Write a balanced equation for this process in words and in formulae: v __________________________________________________________________________________ Carbon dioxide and water to produce oxygen and glucose. Solar energy and chlorophyll must be present. Pictures of chloroplasts in Elodea and cytoplasmic streaming http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopyuk.org.uk/mag/artnov00/dwelodea.html http://www.cells.de/cellseng/1medienarchiv/Zellstruktur/Plastiden/Chloroplasten/CP_in_Protoplasmas troemung/Wasserpest/index.jsp Label the following diagram and electron micrograph of a chloroplast: Biology 12: Cell structure and function Page 16 In living systems, matter moves in cycles, whereas energy flow is non-cyclic. Label the following diagram to illustrate this concept, and to show the relationship between cellular respiration and photosynthesis: Biology 12: Cell structure and function Page 17 The Cytoskeleton (Sec 4.17 p 65 – 66) Provides structural support for the cell and involved in movement in the cell as well as mechanically transmitting cell signals from the cell surface to the interior. The cytoskeleton is made up of various types of long fibres within the cell, composed of ________protein _______ molecules. List the three types of cytoskeleton fibres: Microfilaments, microtubules and intermediate filaments. Here are some cool microtubule photos:http://www.microscopyu.com/moviegallery/sweptfield/folu- egfp-eb3-sfc/ Which of the cytoskeleton fibres are the thinnest? __________________________________ thickest? ___________________________________ What are some of the functions of the cytoskeleton? Microfilaments: are made of actin, a globular protein, and are involved in cell contraction in muscle cells and in amoeboid movement of amoeba and white blood cells. Intermediate filaments: made of fibrous proteins which anchor organelles and maintain cell shape. Microtubules: Hollow tubes which provide rigidity and shape to cells Biology 12: Cell structure and function Page 18 Cilia and flagella (p 66a) are both cellular projections that can move because they contain microtubules. Which molecule provides the energy for the cilia and flagella to move? ATP See Activity: Cilia and Flagella (4.18) List some places in the human body where cilia are found: ___fallopian tube in females and the trachea and nasal passages. What is the only cell in human body that has a flagellum? Sperm cell _____ This diagram shows the common structure of both the cilium and the flagellum: Biology 12: Cell structure and function Page 19 Inner Life of the cell http://www.youtube.com/watch?v=ZRv_dmPKZgw Here is a good review table of some of the various eukaryotic organelles and their main functions: (see Sec 4.23 on p 69) You can review plant and animal cells structure/function with the following web activities: Activity: Review: Animal Cell Structure and Function (4.23) Activity: Review: Plant Cell Structure and Function (4.23) Biology 12: Cell structure and function Page 20