CURRICULUM VITAE
PERSONALPROFILE:
NAME
: Samoel Ashimosi KHAMADI
ADDRESS
: P.O Box 54840-00200, Nairobi, Kenya.
TELEPHONE
: 0722-807841
E-MAIL ADRESS : skhamadi@wrp-t.org; skhamadi@gmail.com
DOMAIN
: ashimosikhamadi.com
MARITAL STATUS: Married
NATIONALITY
: Kenyan
LANGUAGES
: Fluent in English, Kiswahili and Luhya
CAREERASPIRATIONS:
To work diligently in helping address Public Health Challenges by linking appropriate
interventions to common day medical problems.
QUALIFICATIONS SUMMARY:
More than 10 years experience as a Laboratory Scientist. I have been actively involved in HIV
Research with 29 publications in peer reviewed Journals. I have worked closely with the Ministry of
Health through NASCOP in designing and implementing of Public Health interventions in Diagnostics
and HIV Drug Resistance testing. Proficient in the use of MS Office Software, including MS WORD,
EXCEL, POWER POINT and MS Access in daily activities.
EDUCATIONAL BACKGROUND
2008-2009: DIPLOMA IN PROJECT MANAGEMENT I: Kenya Institute of
Management (KIM): Units studied included Financial Management; Research Methods; Legal
Aspects of Project Management; Project Planning Design and Implementation and Information
Systems in Project Management.
2006-2007: POST-DOCTORAL FELLOW: University o f Manitoba/University of
Nairobi, Department of Medical Microbiology, worked on elucidating T-cell responses in HIV
infected and resistant persons in Pumwani, Kenya.
2003-2006:
PHD IN MOLECULAR MEDICINE: Institute of Tropical Medicine Jomo
Kenyatta University of Agriculture and Technology. Defended a Thesis titled, “Molecular
Characterization of HIV Subtypes in the Northern Border Region of Kenya: Cross-Border Effects
and Implication for the Continuing HIV Epidemic in Kenya”.
1997 - 2000:
MASTER OF SCIENCE (M.SC.) IN ZOOLOGY: University Of Nairobi.
Specialized in Parasitology & Immunology. Defended a Thesis in Molecular Biology
Titled: “The Isolation and Characterization of a gene for the T-lymphocyte Triggering Factor (TLTF)
from Trypanosoma vivax.”
1993 - 1996: BACHELOR OF SCIENCE (B.SC.): Jomo Kenyatta University Of
Agriculture And Technology. Specialized in Botany, Zoology and Chemistry.
Signed: 2nd December 2013
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1988 - 1991: KENYA CERTIFICATE OF SECONDARY EDUCATION (KCSE): Kakamega
High School, Kakamega. B plain with 88 points (awarded Best Biological Science Student at
Kakamega High School).
1979 - 1987: KENYA CERTIFICATE OF PRIMARY EDUCATION (KCPE): Lions Primary
School, Kakamega. 65 points out of 72 (awarded Best Pupil at Lions Primary School)
EMPLOYMENT HISTORY
January 14th 2013 to date: US Military HIV Research Program, Henry M. Jackson Foundation
Medical Research International (Walter Reed HIV Program), Tanzania. Research Director.
Main Duties:
1. Support US Military HIV Research Program (MHRP) in carrying out Research in HIV/AIDS.
Direct and manage the development of research based activities at Walter Reed facilities
consistent with the principles of the United States President's Emergency Plan for AIDS Relief
(PEPFAR).
2. Responsible for coordinating and leveraging Walter Reed laboratory research based activities and
expertise with other U.S. government, international and host government agencies as it relates to
the activities supported in Tanzania and East Africa by the DoD/US MHRP.
3. Coordinate laboratory development under PEPFAR with MHRP research conducted in Tanzania
and coordinate research studies occurring in the project.
4. Assist in providing resources technical advice/assistance and analysis for improving laboratory
services with particular emphasis on quality management in the clinical laboratory.
5. Assist to develop the Mbeya Referral hospital referral capabilities in laboratory services and work
with in country USG committees, providing support and undertaking assigned tasks as needed as
part of the overall USG effort under PEPFAR.
September 1 ST 2011 to January 12th 2013: KEMRI/Walter Reed Project. Laboratory
Manager, Department of Emerging Infectious Diseases (DEIDS) Viral Hemorrhagic Fevers
Laboratory, Center for Virus Research, Nairobi Kenya.
Main Duties:
1. Ensure that all activities performed as per available SOPs which should be maintained
regularly
2. Ensure VHF staffs are conversant with study protocols implemented in the Section.
3. Budget for and order all consumables and supplies to support the VHF laboratory
activities.
4. Manage storage of consumables and supplies
5. Ensure that all equipment is in working order and is closely and continuously monitored
per the laboratories SOPs.
6. Monitor the work progress of all VHF Laboratory staff (GEIS and KEMRI) and report to
the Lab director and GEIS program about staff progress.
7. Assist the Lab Director in the training and education of laboratory personnel.
8. Maintain a data management system for all projects and work performed at the VHF
laboratory.
9. Maintain a specimen monitoring and tracking system for all past and future laboratory
specimens
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10. As per the SOPs, maintain, perform and lead a QA/QC and safety program for all
laboratory work.
11. Ensure that proper Biosecurity measures are enacted at the VHF Laboratory.
12. Manage the VHF laboratory in the event of a disease epidemic or other public health
emergency in which case the lab manager will be expected to work overtime and on
weekends.
13. Cross-train with the WRP GEIS Influenza Program and be able to prepare for, lead and
fully manage the VHF laboratory in a surge capacity in support of the Influenza program
in the event of an Avian Flu or pandemic flu outbreak.
14. Comply with any other necessary duty or responsibility as directed by the Lab Director
and GEIS coordinator.
July 1st 2009 to 31st August 2011: KEMRI/Walter Reed HIV Research Project: Deputy
Laboratory Director (College of American Pathologists (CAP) Certified Laboratory)
Main Duties:
1. Oversee and Provide Technical Support in Early Infant Diagnosis (EID) of HIV in the
PEPFAR Program.
2. Oversee and Provide Technical Support in HIV Drug Resistance Testing, Viral Load
Testing and other Molecular and Serological Assays in the Program.
3. Oversee the implementation of New Protocol Assays in the Laboratory.
4. Responsible for assisting the Laboratory Director in the design and implementation of the
Laboratory Quality management program.
5. Ensure that the Clinical Research Centre (CRC) Laboratory produces quality clinical
results by either participating directly in the testing or through supervision.
6. Responsible for supervising all the laboratory functions including but not limited to
clinical laboratory SOP development and maintenance, proficiency testing, phlebotomy
(policy and procedure).
7. Serve as the primary point of contact for all clinical laboratory related issues, results
interpretation and logistics.
8. Represents the CRC laboratory in the development of protocols and other clinic projects.
9. Represents the CRC laboratory in external workshops or conferences as required i.e.
Laboratory administration.
May 2 0 0 2 -to J u n e 30 t h 2009: Senior Research O ff i cer , Ken ya M e d i c a l Res ea r ch
Institute (KEMRI), HIV Research Laboratories, Center For Virus Research. Section Head,
HIV Research Laboratories
Main duties:
1. Carrying out window period studies on transfused blood to monitor blood safety.
2. Performing routine diagnostic HIV work.
3. Carrying out sequence studies on the prevalent HIV strains present in Kenya.
4. Writing Research Protocols in HIV Research.
5. Supervising staff working in the HIV laboratory.
6. Training Personnel on Pediatric HIV Diagnosis Techniques in the Early Infant Diagnosis
Program in Kenya.
7. Team Leader, Pediatric HIV/AIDS Diagnosis Program, KEMRI Nairobi.
8. Assisted in Supervision of Master of Science and PhD students at the Institute of Tropical
Medicine.
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JULY 2001-TO MAY 2002: Research Technologist Grade II: The International
Livestock Research Institute (ILRI): Joined the CIRAD/IRD/ILRI team working on congopainbased vaccine development headed by Dr Edith Authié, under the direct supervision of Dr.
Alain Boulangé. Responsibilities included carrying out experiments on: Nucleic acid extraction and
analysis; DNA Cloning; PCR techniques; Site-directed mutagenesis; Recombinant protein expression
in bacteria, baculovirus and Pichia (yeast) and Protein purification and analysis from the different
expression systems.
JANUARY 1996 – APRIL 1996: Research Intern In Molecular Biology At The
Institute Of Primate Research (IPR): Techniques learnt included SDS Polyacrylamide Gel
Electrophoresis (PAGE); Western Blot assays; Parasitic Diseases Diagnosis and Preparation of
tissue sections for microscopic analysis.
MAY 1997 TO MAY 2003: Volunteer At Uzima Foundation Africa (Charitable Trust):
Responsibilities included organizing community work services involving the youth. These included
Entrepreneurship projects facilitation and facilitation of seminars on HIV/AIDS.
JUNE – AUGUST 1997: Registration Assistant, Glaxo Wellcome (Kenya) Limited:
Responsibilities included preparation of Drug registration and re-registration dossiers; lending out
medical literature in the Company’s Resource Center; Computer work on the Windows 95
packages (MS DOS, MS WORD, MS OFFICE); Medical information searches on MEDLINE, a
computer application package on the CD ROM and Other general office duties.
COLLABORATIONS
1. Centers for Disease Control and Prevention (CDC), National AIDS and STDs Control
Program (NASCOP) and the Clinton Foundation HIV/AIDS Initiative (CHAI): Pediatric
HIV/AIDS Diagnosis Program Scale up in Kenya (2003-2012).
2. University o f S t e l l e n b o s c h : HIV/AIDS M o l e c u l a r R e s e a r c h S t u d i e s
involving s t u d e n t exchange programs (2009-2011).
3. The Caris Foundation International: Provision of testing expertise for the Pediatric HIV
diagnosis Program in Haiti (2006-2009).
4. Medecins sans Frontierés Spain, and Foundation for Biomedical Investigation, Terrassa Mutua
Hospital Spain: HIV Drug resistance testing using Dried Blood Spots in Busia District
Hospital (2009-2012).
5. Safaricom Foundation, Kenya: Expanding Pediatric HIV Diagnosis Program in Kenya by use
of Cell phones Networks (2006-2012).
6. African Population and Health Research Centre (APHRC): The Economic, Health, and Social
Context of HIV infection in Informal Urban Settlements of Nairobi (2007-2012).
7. The Global Health Initiative (GHI) in Developing the Hitsystem Software for follow-up
ofHIV-exposed infants in Kenya (2009 to date).
CONSULTANCIES
1. Consulted for AMREF in the development of E-learning Materials on HIV/AIDS for
Laboratory Personnel (2010).
2. Consulted for World Health Organization (WHO) and the National AIDS and STIs Control
Programme (NASCOP) in the development of the HIV Drug Resistance report for Kenya
(2010-2011).
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3. Consulted for The Clinton Foundation and the National AIDS and STIs Control Programme
(NASCOP) in the development of Training materials and Field and Laboratory Standard
Operating
Procedures (SOPs) for Early Infant Diagnosis (EID) of HIV in Kenya.
INCOUNTRYRESPONSIBLITIES SERVED
1.
2.
3.
4.
5.
Member of the HIV Drug Resistance Technical Working Group.
Member of the Early Infant Diagnosis (EID) of HIV in Kenya Technical group.
Involved in the design of SOPs for rollout of Viral Load testing in Kenya.
Member of the Kenya HIV Vaccine Steering group.
Trainer in the Gold Star Network on Laboratory Testing Procedures.
PUBLICATIONS
1. Bosire EM, Nyamache AK, Gicheru MM, Khamadi SA, Lihana RW, Okoth V. Population
specific reference ranges of CD3, CD4 and CD8 lymphocyte subsets among healthy Kenyans.
AIDS Res Ther. 2013 Nov 7;10(1):24.
2. Muriuki BM, Gicheru MM, Wachira D, Nyamache AK, Khamadi SA. Prevalence of hepatitis
B and C viral co-infections among HIV-1 infected individuals in Nairobi, Kenya. BMC Res
Notes. 2013 Sep 9;6:363.
3. Lutomiah J, Bast J, Clark J, Richardson J, Yalwala S, Oullo D, Mutisya J, Mulwa F, Musila
L, Khamadi S, Schnabel D, Wurapa E, Sang R. Abundance, diversity, and distribution of
mosquito vectors in selected ecological regions of Kenya: public health implications. J Vector
Ecol. 2013 Jun;38(1):134-42.
4. Ochieng C, Lutomiah J, Makio A, Koka H, Chepkorir E, Yalwala S, Mutisya J, Musila
L, Khamadi S, Richardson J, Bast J, Schnabel D, Wurapa E, Sang R. Mosquito-borne
arbovirus surveillance at selected sites in diverse ecological zones of Kenya; 2007-2012. Virol
J. 2013 May 10;10(1):140
5. Nyamache AK, Muigai AW, Ng'ang'a Z, Khamadi SA. Profile of HIV type 1 coreceptor
tropism among Kenyan patients from 2009 to 2010. AIDS Res Hum Retroviruses. 2013
Aug;29(8):1105-9.
6. Arnedo M, Alonso E, Eisenberg N, Ibáñez L, Ferreyra C, Jaén A, Flevaud L, Khamadi S,
Roddy P, Gatell JM, Dalmau D; Busia O R Study Group. Monitoring HIV viral load in
resource limited settings: still a matter of debate? PLoS One. 2012;7(12):e47391.
7. Ferreyra C, Yun O, Eisenberg N, Alonso E, Khamadi AS, Mwau M, Mugendi MK, Alvarez
A, Velilla E, Flevaud L, Arnedo M, Dalmau D, Roddy P, Bernasconi A, Palma PP. Evaluation
of clinical and immunological markers for predicting virological failure in a HIV/AIDS
treatment cohort in Busia, Kenya. PLoS One. 2012;7(11):e49834.
8. Nyamache AK, Muigai AW, Khamadi SA. Circulating trends of non-B HIV type 1 subtypes
among Kenyan individuals. AIDS Res Hum Retroviruses. 2013 Feb;29(2):400-3.
9. Masciotra S, Khamadi S, Bilé E, Puren A, Fonjungo P, Nguyen S, Girma M, Downing R,
Ramos A, Subbarao S, Ellenberger D. Evaluation of blood collection filter papers for HIV-1
DNA PCR. J Clin Virol. 2012 Jul 7.2.
10. Madise N, Ziraba AK, Inungu J, Khamadi SA, Ezeh A, Zulu EM, Kebaso J, Okoth V,
Mwau M. Are slum dwellers at heightened risk of HIV infection than other urban residents?
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Evidence from population-based HIV prevalence surveys in Kenya. Health Place. 2012 Apr
26.
11. Sivapalasingam S, Ahmed A, Mendillo M, Holzman R, Marshed F, Mwamzuka
M, Khamadi S, Mwau M, Laverty M, Valentine F. Early Detection of HIV Infection among
Kenyan Infants Using a Reverse Transcriptase Activity Assay. Pediatr Infect Dis J. 2012
Jul;31(7):732-5.
12. Muriuki JK, Ngeranwa J, Mwangi JM, Orinda G, Lwembe R, Khamadi SA. Isolation and
Biological Characterization of Non B HIV-1 from Kenya. AIDS Res Hum Retroviruses.
2011 Nov 14.
13. A. K. NYAMACHE, R. WAIHENYA, Z. W. NG'ANG'A , A. W. MUIGAI and S. A.
KHAMADI. REVERSE TRANSCRIPTASE INHIBITORS DRUG RESISTANCE
MUTATIONS IN DRUG-NAIVE HIV TYPE 1 POSITIVE KENYANS East African Medical
Journal January 2011.
14. Kageha S, Okoth V, Kadima S, Vihenda S, Okapesi E, Nyambura E, Maiyo A, Ndung'u N,
Khamadi S, Mwau M. Discrepant test findings in Early Infant Diagnosis of HIV in a
National Reference Laboratory in Kenya: Challenges and Opportunities for Programs. J Trop
Pediatr. 2011 Nov 3.
15. Nyamache AK, Muigai A, Nganga Z, Khamadi SA. HIV-1 genetic diversity and naturally
occurring polymorphisms in HIV-1 Kenyan isolates: Implications for integrase inhibitors.
AIDS Res Hum Retroviruses. 2011 Sep 15.
16. Nyagaka B, Kiptoo MK, Lihana RW, Khamadi SA, Makokha EP, Kinyua JG, Mwangi J,
Osman S, Lagat NJ, Muriuki J, Okoth V, Gicheru M, Ng'ang'a Z, Songok EM.HIV Type 1 gag
Genetic Diversity Among Antenatal Clinic Attendees in North Rift Valley, Kenya. AIDS Res
Hum Retroviruses. 2011 Sep 7
17. Kageha S, Lihana RW, Okoth V, Mwau M, Okoth FA, Songok EM, Ngaira JM, Khamadi
SA. HIV Type 1 Subtype Surveillance in Central Kenya. AIDS Res Hum Retroviruses. 2011
Jul 8.
18. Tamhane M, Gautney B, Shiu C, Segaren N, Jeannis L, Eustache C, Simeon-Fadois Y, Chen
YH, De D, Irivinti S, Tamma P, Thompson CB, Khamadi S, Siberry GK, Persaud D.
Analysis of the optimal cut-point for HIV-p24 antigen testing to diagnose HIV infection in
HIV-exposed children from resource-constrained settings. J Clin Virol. 2011 Apr;50(4):33841. Epub 2011 Feb 16.
19. Boulangé AF, Khamadi SA, Pillay D, Coetzer TH, Authié E. Production of congopain, the
major cysteine protease of Trypanosoma (Nannomonas) congolense, in Pichia pastoris
reveals unexpected dimerisation at physiological pH. Protein Expr Purif. 2011 Jan;75(1):95103. Epub 2010 Sep 7.
20. Ziraba AK, Madise NJ, Matilu M, Zulu E, Kebaso J, Khamadi S, Okoth V, Ezeh AC. The
effect of participant nonresponse on HIV prevalence estimates in a population-based survey
In two informal settlements in Nairobi city. Popul Health Metr. 2010 Jul 22; 8:22.
21. Lihana RW, Khamadi SA, Lubano K, Mwangi J, Kinyua JG, Okoth VO, Lagat NJ, Okoth
FA, Songok EM, Makokha EP. Genetic characterization of HIV type 1 among patients with
suspected immune reconstitution inflammatory syndrome after initiation of antiretroviral
therapy in Kenya. AIDS Res Hum Retroviruses. 2010 Jul;26(7):833-8.
22. Lihana RW, Khamadi SA, Lwembe RM, Kinyua JG, Muriuki JK, Lagat NJ, Okoth FA,
Makokha EP, Songok EM. HIV-1 subtype and viral tropism determination for evaluating
antiretroviral therapy options: an analysis of archived Kenyan blood samples. BMC Infect
Dis. 2009 Dec 30;9:215.PMID: 20040114.
23. Lihana RW, Khamadi SA, Lubano K, Lwembe R, Kiptoo MK, Lagat N, Kinyua JG, Okoth
FA, Songok EM, Makokha EP, Ichimura H. HIV type 1 subtype diversity and drug
resistance among HIVtype1-infected Kenyan patients initiating antiretroviral therapy. AIDS
Res Hum Retroviruses. 2009 Dec;25(12):1211-7.PMID: 19954302.
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24. Lihana RW, Khamadi SA, Lwembe RM, Ochieng W, Kinyua JG, Kiptoo MK, Muriuki JK,
Lagat N, Osman S, Mwangi JM, Okoth FA, Songok EM. The changing trend of HIV type 1
subtypes in Nairobi. AIDS Res Hum Retroviruses. 2009 Mar; 25(3):337-42.
25. Kebira, A.N, Ochola P and Khamadi, S.A. Isolation and antimicrobial susceptibility testing
of Escherichia coli causing urinary tract infections. Journal of Applied Biosciences October
2009. 22: 1320 – 1325.
26. Khamadi SA, Lihana RW, Osman S, Mwangi J, Muriuki J, Lagat N, Kinyua J, Mwau M,
Kageha S, Okoth V, Ochieng W, Okoth FA. Genetic diversity of HIV type 1 along the
coastal strip of Kenya. AIDS Res Hum Retroviruses. 2009 Sep;25(9):919-23.PMID:
19751145.
27. Mwangi J, Nganga Z, Songok E, Kinyua J, Lagat N, Muriuki J, Lihana R, Khamadi S,
Osman S, Lwembe R, Kiptoo M, Mwau M, Chirchir R, Mpoke S, Nyamongo J, Okoth F,
Yamada R, Kageyama S, Ichimura H. Molecular Genetic Diversity of Hepatitis B Virus in
Kenya. Intervirology. 2009 Mar 4;51(6):417-421
28. Khamadi SA, Lihana RW, Mwaniki DL, Kinyua J, Lagat N, Carter JY, Ichimura H, Oishi I,
Okoth FA, Ochieng W. HIV type 1 genetic diversity in Moyale, Mandera, and Turkana
based on env-C2-V3 sequences. AIDS Res Hum Retroviruses. 2008 Dec;24(12):1561-4.
29. Khamadi S, Okoth V, Lihana R, Nabwera J, Hungu J, Okoth F, Lubano K, Mwau M.
Rapid Identification Of Infants For Antiretroviral Therapy In A Resource Poor Setting: The
Kenya Experience. Journal of Tropical Pediatrics, 2008 Dec;54(6):370-4.
30. Khamadi SA, Ochieng W, Lihana R.W, Lwembe R, Kiptoo M.K, Kinyua J, Lagat N,
Muriuki J, Mwangi J, Oishi I, Ichimura H, Mpoke S and Songok E.M; HIV type 1 subtypes
in circulation in northern Kenya. AIDS Res Hum Retroviruses. 2005 Sep; 21(9): 810-4.
31. Lihana RW, Khamadi SA, Kiptoo MK, Kinyua JG, Lagat N, Magoma GN, Mwau MM,
Makokha EP, Onyango V, Osman S, Okoth FA, Songok EM. HIV type 1 subtypes among
STI patients in Nairobi: a genotypic study based on partial pol gene sequencing. AIDS Res
Hum Retroviruses. 2006 Nov;22(11):1172-7.
SELECTED CONFERENCESATTENDED
1. 15th International AIDS Conference, Thailand: 11-16 July 2004. Presented a poster on
“HIV-1 subtypes circulating in Northern Kenya”.
2. 25th African Health Science Congress, Kenya: 4-8 October 2004. Presented a paper on
“HIV-1 subtype Diversity in Border regions of Northern Kenya”.
3. 3rd Wellcome T r u s t /EMBO Workshop on HIV/AIDS & TB, South Africa: 1-8
December 2004. Presented a paper on “the Dynamics of HIV-1 subtype Diversity in Kenya”.
4. Covenant University International Workshop on Pattern Discovery In Biology April 18
- 27, 2005, Nigeria.
5. HIV/AIDS Implementers meeting June 3-8, 2008. Attended as a delegate.
6. Roche Annual Diagnostic Forum, Johannesburg, South Africa: 20th October 2008.
7. International AIDS Vaccine Conference, Cape town, South Africa: 13th to 18th October
2008.
OTHERTRAININGS AND QUALIFICATION
1. Division of AIDS (DAIDS) Clinical Trials Training on GCP and GCLP, Kampala
Uganda, 2009.
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2. GCLP and GLP Training 2007, at Family Health International Research Ethics Training
Curriculum 2007, at Family Health International Training on EID Techniques, at CDC Kisumu,
2006
3. ISO training 15189 and 19011 for Implementation and Conducting of Audits in the
Laboratory.
OTHERDUTIES RESPONSIBILITESANDACHIEVEMENTS
1. External Examiner: Nairobi University Department of Zoology; Jomo Kenyatta University of
Agriculture and Technology.
2. Supervised over 30 Master of Science and PhD Students from different Universities in
Kenya.
PERSONALINTERESTS
1.
2.
3.
4.
Playing and watching soccer
Sight-seeing
Reading Christian and Educational literature
Swimming
REFEREES:
Dr. Irene Mukui,
ART Programme Manager, NASCOP.
P.O. Box 19361-0202 Nairobi,
Tel: 0722387986
Email: njahira2004@yahoo.com
Dr. David Odongo,
Senior Scientist, International Livestock Research Institute (ILRI),
P.O Box 30709, Nairobi, Kenya.
Tel:0722984090
Email: d.odongo@cgiar.org
Professor Horace Ochanda,
Department of Zoology,
University Of Nairobi
Po Box 30197, Nairobi,
TEL: 0722228231
Email: hochanda@uonbi.ac.ke
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