In vivo spermatotoxic effect of chromium as reflected in the epididymal
epithelial principal cells, basal cells, and intralepithelial macrophages of
a nonhuman primate (Macaca radiata Geoffroy)
By:Aruldhas, MM (Michael Aruldhas, Mariajoseph); Subramanian, S (Subramanian, Senthivinayagam); Sekhar,
P (Sekhar, Pasupathi); Vengatesh, G(Vengatesh, Ganapathi); Govindarajulu, P (Govindarajulu, Peranaidu); Akbarsha,
MA (Abdulkader Akbarsha, Mohammad)
FERTILITY AND STERILITY
Volume: 86
Pages: 1097-1105
Supplement: 3
DOI: 10.1016/j.fertnstert.2006.03.025
Published: OCT 2006
View Journal Information
Abstract
Objective: To understand, through a simulation experiment in a nonhuman primate model, the potential in vivo
spermatotoxic effect of hexavalent chromium (CrVI) in men who are occupationally or environmentally exposed to it.
Design: Controlled laboratory study.
Setting: Research laboratory in a department of endocrinology in a university in India.
Animal(s): Male bonnet monkey, Macaca radiata Geoffrey.
Intervention(s): Monkeys were exposed ad libitum to 100, 200 and 400 ppm CrVI, dissolved in drinking water, for a
chronic period of 180 days.
Main Outcome Measures: Examination of epididymis with a transmission electron microscope and assessment of the
effect of CrVI in terms of accumulation of sperm-derived lipofuscin (LF) material in the principle cells, basal cells and
intrapethial macrophages of the epithelium.
Result(s): The abundance of basal cells and intrapethial macrophages and the content of LF material in these cell types
increased. The principal cells phagocytosed from the lumen the dead sperm resulting from CrVI exposure and
processed them partially into LF material, which was acquired by the basal cells and intrapethial macrophages and
processed further. The LF-laden material basal cells and intrapethial macrophages appeared to leave the epithelium,
accompanied by recruitment of fresh basal cells and intrapethial macrophages.
Conclusion(s): Occupational or environmental exposure to CrVI, as would occur in the tannery, soap and other
industries in developing and underdeveloped countries, can be toxic in vivo to spermatozoa.
Keywords
Author Keywords:basal cell; chromium; epididymis marcophage; lipofuscin material; principal cell; spermatotoxicity
KeyWords Plus:HEXAVALENT CHROMIUM; MURINE EPIDIDYMIS; TESTICULAR TISSUE; SEX-HORMONES; VASDEFERENS; EXPOSURE; RATS;EXPRESSION; TOXICITY; OBSTRUCTION
Author Information
Reprint Address: Aruldhas, MM (reprint author)
Univ Madras, Dr ALM PostGrad Inst Basic Med Sci, Dept Endocrinol, Taramani Campus, Madras 600113, Tamil Nadu, India.
Addresses:
[ 1 ] Univ Madras, Dr ALM PostGrad Inst Basic Med Sci, Dept Endocrinol, Madras 600113, Tamil Nadu, India
[ 2 ] Bharathidasan Univ, Sch Life Sci, Dept Anim Sci, Tiruchchirappalli, India
E-mail Addresses:aruldhasmm@yahoo.com
Author Identifiers:
Publisher
ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
Categories / Classification
Research Areas:Obstetrics & Gynecology; Reproductive Biology
Web of Science Categories:Obstetrics & Gynecology; Reproductive Biology
Document Information
Document Type:Article
Language:English
Accession Number: WOS:000241289300009
PubMed ID: 16949592
ISSN: 0015-0282
Other Information
IDS Number: 095DV
Cited References in Web of Science Core Collection: 32
Times Cited in Web of Science Core Collection: 7