ele12272-sup-0004-Supplementarymaterial

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1 Supplementary Figures and Tables

2 Table S1. The influence of local environmental variables on first and second year population

3 success and overwinter survival for three species relocated to cooler topographic microclimates.

4 F-statistics and P-values are from a forward selecting stepwise model where we selected the

5 model with the lowest AIC score.

6

7

Species Plot predictor variable First year Overwinter Survival Second year

Horkelia Slope F

1,84

=5.32, P=0.02

F

1,83

=6.30, P=0.01 n.s.

Soil moisture F

1,84

=4.24, P=0.04 F

1,83

=23.87, P<0.01 F

1,84

=9.49, P<0.01

Arabis Slope

Soil moisture

Soil Strength

Soil Strength

Canopy Density

F

1,84

=5.72, P=0.02 F

1,83

=9.78, P=0.002 F

1,84

=6.04, P=0.02

F

1,84

Number of trees/shrubs n.s.

=5.46, P=0.02 n.s. n.s. n.s. n.s.

Canopy Density

Number of trees/shrubs

F

F

1,44 n.s. n.s. n.s.

1,44

=2.73, P=0.10

=9.24, P<0.01 n.s. n.s. n.s. n.s. n.s.

Phacelia Slope

Soil moisture

Soil Strength

Canopy Density n.s.

F

1,49 n.s.

n.s.

Number of trees/shrubs n.s. n.s.

=3.69, P=0.06 n.s. n.s. n.s. n.s.

No survivors

No survivors

No survivors

No survivors

No survivors n.s. n.s. n.s. n.s. n.s.

8

9

10

11

12

13

14

15

16

17

18 Figure S1. (a) Geology of the study area (modified after Ramp & Peterson 1979). Upper left

19 inset: study area location near Oregon-California border (after Irwin 1966, 1981). Inset key: CM,

20 central metamorphic belt; EK, eastern Klamath belt; KJ, Cretaceous and Jurassic rocks

21 undifferentiated; QT, Quaternary and Tertiary rocks undifferentiated; WJ, western Jurassic belt;

22 WMP, western Mesozoic and Paleozoic belt. Map key: Ks, Cretaceous sedimentary rocks; Js,

37

38

39

40

41

32

33

34

35

36

23 Jurassic metasedimentary rocks; Jvs, Jurassic metasedimentary and metavolcanic rocks; JTrvs,

24 Early Jurassic and Triassic metasedimentary and metavolcanic rocks; TQs, Tertiary (minor) and

25 Quaternary sediments; UM, ultramafic and related rocks, includes peridotite and serpentinite.

26 Heavy weight lines with barbs (dashed or dotted where uncertain or hidden) indicate thrust

27 faults; Dashed line, highway.

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29

30

31

42

43

44

45

First year

1.50

1.25

(a) Phacelia

N

S

X

1.00

0.75

0.50

0.25

0.00

400 600

Overwinter

100 (d) Phacelia

75

N

S

X

800

0.25

0.20

(b) Horkelia

N

S

X

0.15

0.10

1000 1200

0.05

1400

0.00

400 600 800 1000 1200

0.30

(c) Arabis

0.25

0.20

0.15

0.10

0.05

1400

0.00

400 600

100

75

(e) Horkelia

N

S

X

100

75

(f) Arabis

N

S

X

800 1000 1200 1400

N

S

X

50 50 50

25 25 25

0

400 600

Second year

1.50

1.25

(g) Phacelia

N

S

X

1.00

800

0.75

0.50

0.25

0.00

400 600

1000 1200

800 1000 1200

Elevation (m)

1400

0

400 600 800 1000 1200 1400

0

400 600

0.8

0.6

(h) Horkelia

N

S

X

X

0.4

0.2

1400

0.0

400 600 800 1000

Elevation (m)

1200

0.30

(i) Arabis

0.25

0.20

0.15

0.10

0.05

1400

0.00

400 600

800 1000 1200 1400

N

S

X

800 1000

Elevation (m)

1200 1400

46

47 Figure S2. First year population success (a-c) calculated as summed biomass of all individuals

48 within a plot), percent overwinter survival (d-f) and second year population success (g-i) in

49 relation to aspect and elevation in neighbor removal sub-plots. For graphing purposes we present

50 site means to better illustrate patterns, but analyses were conducted on plot data. A significant

51 positive relationship between population success and elevation and/or a significantly higher

52 population success on north-facing aspects indicates that relocated species do better in cooler

53 environments. Vertical dashed lines represent current upper elevation range limit for the three

54 target species; North-facing aspects (N: white symbols, black dashed line) south-facing aspect

55 (S: grey symbols, grey line) and non-aspect sites (X: black symbols); Black solid lines represent

56 regressions when all three aspects (N, S, X) are combined.

30 (a) Phacelia

25

20

15

10

5

0

400 600 800

0.50

0.25

(d) Phacelia

N

S

X

N

S

X

1000 1200 1400

80

70

(b) Horkelia

60

50

40

30

20

10

0

400 600 800

0.50

(e) Horkelia

0.25

N

S

X

30 (c) Arabis

25

20

15

10

5

0

400 600 1000 1200 1400

N

S

X

0.50

(f) Arabis

0.25

N

S

X

800 1000 1200 1400

N

S

X

0.00

-0.25

0.00

-0.25

0.00

-0.25

57

58

-0.50

400 600 800 1000 1200 1400

-0.50

400

Elevation (m)

600 800 1000 1200 1400

Elevation (m)

-0.50

400 600 800 1000 1200 1400

Elevation (m)

Figure S3. Mean species responses at early life stages (% germination and logRR at germination)

59 on north-facing (white circles, dashed lines) south-facing (grey circles, grey line) and non-aspect

60 (black circles) sites along the elevation gradient. Here we used the same analysis (GLMM:

61 aspect, elevation, and the interaction between aspect and elevation, site as a random factor) as we

62 did with population success. Horkelia and Phacelia germination were higher on north aspects >

63 700m. Arabis responses were not strongly influenced by aspect, but were influenced by elevation

64 with little germination or survival and lower biomass at higher elevations. Neighbors had a

65 positive effect on the germination of Phacelia at higher elevations. Neighbors had no effect on

66 germination of Horkelia or Arabis . Black vertical dashed lines show upper elevational range

67 limit for each species. For graphing purposes we present site means to better illustrate patterns,

68 but analyses were conducted on plot data.

69

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