Name_____________________ Block_____________________ Surface Area to Volume Ratio Investigation Materials: Agar blocks Container of household ammonia (Enough to cover the agar blocks in the containers) 1 Labquest 2 temperature probes 250 mL beaker 50 mL beaker ½ of petri dish 100 mL graduated cylinder Part 1 1. Create a data table that includes labels for surface area, volume, surface area to volume ratio (SA/V), time, distance ammonia traveled, and volume of cube unaffected by ammonia. (This should be placed in a Google doc along with your graph for part 2 and shared with me in your IB Biology Folder!) 2. Obtain and cut three blocks of agar from your instructor, making sure each block is a different sized cube. 3. Measure and calculate the surface area and volume of each cube and record this in your data table 4. Place blocks into household ammonia; let sit for 5-10 minutes 5. Remove blocks and rinse with water, then cut each block in half. 6. Measure the volume inside of the cube that was not affected by ammonia. If the ammonia diffused all the way through the block, then the volume receiving no diffused particles would be 0.00 cm3 Observations 1. Describe what the ammonia used in the lab represents in the cell. 2. As the size of the cell increases, which increases faster: cell surface area or cell volume? Explain. 3. As cell size increases, describe what happens to the surface area to volume ratio. 4. Based on your understanding of cell size and the rate (speed) of diffusion, explain why there are generally no extremely large cells. 5. Give an example of a cell that is designed to maximize the surface area to volume ratio and explain how the design benefits that type of cell. Part 2 1. Obtain the bottom portion of a petri dish and a 50 mL beaker. 2. Using the hot plate, heat up 100 mL of water to near boiling. 3. Pour 30mL of the hot water into the petri dish and 30 mL into the 50 mL beaker. 4. Using the lab quest and two separate temperature probes, place one temperature probe into each container of water and measure the temperature of the water for 10 minutes. Make sure that the Labquest is set to take a temperature reading once every minute for ten minutes (you should end up with 10 total data points). 5. Create a graph of your data by exporting the data into logger pro on your computer. Export this graph into your lab document by taking a screen shot and inserting the image. Observations 1. Calculate the total percent loss of temperature for both the beaker and the petri dish. Make sure to show your work. 2. State which container lost the most heat and explain why this occurred. 3. Suggest how this demonstration could apply to living organisms and their ability to maintain body heat.