CRUK Beatson Institute Risk Assessment
Assessor
Margaret O'Prey
Assessment Number
BAIR facility
To be used for significant hazards only
“R” Research Groups: ... Other groups “DDU”, “M”, “Y”, etc. Y60
Assessment Date 14/11/2014
Review Date 14/11/2017
Assessment Title Use of the Nanosight partical counter
Process Hazards Biological Agents
Electrical
Any other Process risks chemicals, inexperience,
Laser
Summary of Work/Process
The Nanosight is a microscope which uses a laser to measure the size of particals that have
been extracted from culture cells and are in a solution of media or buffer. The particals are
filtered and syringed into the flow chamber the laser then detects the particals and they are
captured by the camera and can be viewed and mesured in the software.
Work location
Involves
BAIR level1 room 130
Carcinogens
Radioactive substances
Biological work
Additional Risk Control Measures because of this involvement
Groups at risk
Self
Immediate lab staff
Cleaning Staff
BSU Personnel
Central Services Staff
Visitors
Environment
Containment
Fume cupboard
Microbiological Safety Cabinet
Radiation Containment
Other Containment Arrangements
See comments
COMMENTS:
Personal Protective Equipment
Laboratory coat
Overalls
Safety Glasses
Gloves
Respirator
Specialist footwear
Other*
SPECIFICALLY
Goggles
Face shield
Radiation dosimeter
Any Additional Risk Control Measures
yes
SPECIFICALLY
Training required before using this equipment. Videos on how to use
microscope and set up flow cell available. Safety cut off in place for when cleaning the
unit.
e.g. any required checks on control measures and their frequency; health surveillance, etc.,
Save Date 09/02/2016 04:35:00
Health Surveillance choose
Substances involved
Substance 1
70-% ethanol
COSHH code 1
Frequency of use Daily
Exposure time Minutes
Exposure Limit
Route of hazardous exposure Select route
Hazard “H” codes (to complete with correct code CLICK HERE to skip to bottom of page and
copy & paste)
H302 + H312 + H332 Harmful if swallowed, in contact with skin or if inhaled
H226 Flammable liquid and vapour
H316 Causes mild skin irritation
H320 Causes eye irritation
H335 May cause respiratory irritation
Handling of substance
Substance 2
Q-connect spray duster
COSHH code 1
Frequency of use select
Exposure time Select
Exposure Limit
Route of hazardous exposure Select route
Hazard “H” codes (to complete with correct code CLICK HERE and copy & paste)
H222 Extremely flammable aerosol
Skin contact: There may be mild irritation at the site of contact.
Eye contact: There may be irritation and redness.
Ingestion: There may be irritation of the throat.
Inhalation: There may be irritation of the throat with a feeling of tightness in the chest
[
Handling of substance Store in cool, well ventilated area. Keep container tightly closed.
Keep away from sources of
ignition.
Substance 3
Particles from culture cell
COSHH code 0
Frequency of use Daily
Exposure time Select
Exposure Limit
Route of hazardous exposure Select route
Hazard “H” codes (to complete with correct code CLICK HERE and copy & paste)
Samples that are brought down to the microscope by users. There will possibly have
different risk factors depending on the cells the particles have come from. Users need to
assess this and write up SOP/ risk.
Save Date 09/02/2016 04:35:00
Handling of substance
Substance 4
ethanol 70%
COSHH code 1
Frequency of use Daily
Exposure time Seconds
Exposure Limit
Route of hazardous exposure Select route
Hazard “H” codes (to complete with correct code CLICK HERE and copy & paste)
H303 + H313 + H333 May be harmful if swallowed, in contact with skin or if inhaled
H315 + H320 Causes skin and eye irritation
H226 Flammable liquid and vapour
Handling of substance
More Substances Used!
See additional sheet
Biological Agents
Will be working with:
Primary human cells (tissues, blood etc)
Samples with a blood borne virus risk
Viral vectors (Adenovirus, Lentivirus, Retrovirus)
Will need to use sharps with above biological agents
Spillage / uncontrolled release
Specify the procedure for containing a spillage / release of chemicals or intermediates.
clean area with 70% etoh. dispose of any cellylar material in cinbin for biological disposal.
Equipment
Identify equipment nanosight microscope and pump syringe
used
All the equipment required for this process has been checked
for safe operation prior to use.
YES
NO
Relevant safety locks are working and in place YES
NO
Risk reduction measures must be trained before using this microscope
Fire Precautions
Specify the appropriate firefighting equipment required.
Not Applicable
CO2 (Black)
Foam (Cream)
Water (Red)
Dry Powder (Blue)
Fire Blanket
Sand
Other*
*Specify Comments here
First Aid
Note: this form should be taken with injured person(s) where medical attention is required.
Specify first aid treatment or any special antidotes…
Eyes: Comments Comments
Save Date 09/02/2016 04:35:00
Skin: Comments Comments Comments
Mouth (Ingestion): Comments Comments
Mouth (inhalation): Comments Comments
Further details: Comments
Radiation
please note: the special risk assessment (see intranet.) is required for radiation work with
unsealed sources.
Radiation type Laser *Other add info if required
Radionuclide to be used Choose Activity MBq
Chemical Form
Monitor used Choose
Frequency of use Daily
Duration of use Seconds
Disposal of radioactive waste
Radioactive Waste as a % Solid / Liquid
as determined by Scintillation count
9% Solid
91% liquid
Disposal of Waste
COSHH 3 chemicals and tips into Blue topped sharps bins
Specify Chemical Waste
No1
Storage arrangements prior to disposal
1
No2
2
No.3
3
Disposal arrangements
Chemical destruction / Neutralisation
related COSHH risk assessment
Disposal via contractor
other details
Degree of Supervision
Work carried out by
training requites before use
A
Work may not be carried out without
direct personal supervision by:
Level of supervision required
A
Signed by user:
Signed by user:
Signed by user:
Signed by user:
B
Work may not be started without the
advice and approval of
A member of BAIR staff
C
Work (other than categories A&B) where
it is considered by the project supervisor that
the individual is adequately trained & competent
in the procedures involved
ASSESSMENT APPROVED by:
Save Date 09/02/2016 04:35:00
Date Approved:
Save Date 09/02/2016 04:35:00
Select one or more “H” (Hazard Codes) to enter in
“Substance Hazard boxes” on form
Click here to return to “Substances” once you have copied text
Combinations
H300 + H310 Fatal if swallowed or in contact with skin
H300 + H330 Fatal if swallowed or if inhaled
H310 + H330 Fatal in contact with skin or if inhaled
H300 + H310 + H330 Fatal if swallowed, in contact with skin or if inhaled
H301 + H311 Toxic if swallowed or in contact with skin
H301 + H331 Toxic if swallowed or if inhaled
H311 + H331 Toxic in contact with skin or if inhaled
H301 + H311 + H331 Toxic if swallowed, in contact with skin or if inhaled
H302 + H312 Harmful if swallowed or in contact with skin
H302 + H332 Harmful if swallowed or if inhaled
H312 + H332 Harmful in contact with skin or if inhaled
H302 + H312 + H332 Harmful if swallowed, in contact with skin or if inhaled
H303 + H313 May be harmful if swallowed or in contact with skin
H303 + H333 May be harmful if swallowed or if inhaled
H313 + H333 May be harmful in contact with skin or if inhaled
H303 + H313 + H333 May be harmful if swallowed, in contact with skin or if inhaled
H315 + H320 Causes skin and eye irritation
Individual phrases
H200 Unstable explosive
H201 Explosive; mass explosion hazard
H202 Explosive; severe projection hazard
H203 Explosive; fire, blast or projection hazard
H204 Fire or projection hazard
H205 May mass explode in fire
H220 Extremely flammable gas
H221 Flammable gas
H222 Extremely flammable aerosol
H223 Flammable aerosol
H224 Extremely flammable liquid and vapour
H225 Highly flammable liquid and vapour
H226 Flammable liquid and vapour
H227 Combustible liquid
H228 Flammable solid
H240 Heating may cause an explosion
H241 Heating may cause a fire or explosion
H242 Heating may cause a fire
H250 Catches fire spontaneously if exposed to air
H251 Self-heating; may catch fire
Save Date 09/02/2016 04:35:00
H252 Self-heating in large quantities
H260 In contact with water releases flammable gases which may ignite spontaneously
H261 In contact with water releases flammable gas
H270 May cause or intensify fire; oxidizer
H271 May cause fire or explosion; strong oxidizer
H272 May intensify fire; oxidizer
H280 Contains gas under pressure; may explode if heated
H281 Contains refrigerated gas; may cause cryogenic burns or injury
H290 May be corrosive to metals
H300 Fatal if swallowed
H301 Toxic if swallowed
H302 Harmful if swallowed
H303 May be harmful if swallowed
H304 May be fatal if swallowed and enters airways
H305 May be harmful if swallowed and enters airways
H310 Fatal in contact with skin
H311 Toxic in contact with skin
H312 Harmful in contact with skin
H313 May be harmful in contact with skin
H314 Causes severe skin burns and eye damage
H315 Causes skin irritation
H316 Causes mild skin irritation
H317 May cause an allergic skin reaction
H318 Causes serious eye damage
H319 Causes serious eye irritation
H320 Causes eye irritation
H330 Fatal if inhaled
H331 Toxic if inhaled
H332 Harmful if inhaled
H333 May be harmful if inhaled
H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled
H335 May cause respiratory irritation
H336 May cause drowsiness or dizziness
H340 May cause genetic defects
H341 Suspected of causing genetic defects
H350 May cause cancer
H351 Suspected of causing cancer
H360 May damage fertility or the unborn child
H361 Suspected of damaging fertility or the unborn child
H362 May cause harm to breast-fed children
H370 Causes damage to organs
H371 May cause damage to organs
H372 Causes damage to organs through prolonged or repeated exposure
H373 May cause damage to organs through prolonged or repeated exposure
H400 Very toxic to aquatic life
H401 Toxic to aquatic life
H402 Harmful to aquatic life
H410 Very toxic to aquatic life with long lasting effects
H411 Toxic to aquatic life with long lasting effects
Save Date 09/02/2016 04:35:00
H412 Harmful to aquatic life with long lasting effects
H413 May cause long lasting harmful effects to aquatic life
H420 Harms public health & environment by destroying ozone in upper atmosphere
Save Date 09/02/2016 04:35:00