Project title: Improving Strawberry Plant Establishment In Used Beds Project number: SF 108 Project leader: Robert Irving, ADAS UK Ltd Report: Final report, January 2011 Previous report Annual report, January 2010 Key staff: Robert Irving Location of project: J W Busby & Partners, Manor Farm, Chilcote, Swadlincote, Derbyshire, DE12 8DL Project coordinator: Mr S McGuffie, New Farm Produce, Nr. Lichfield, Staffs, WS13 8EX Date project commenced: 1st February 2009 Date project completed (or expected 31st January 2011 completion date): Key words: Strawberry, soil, establishment, root treatment. Whilst reports issued under the auspices of the HDC are prepared from the best available information, neither the authors nor the HDC can accept any responsibility for inaccuracy or liability for loss, damage or injury from the application of any concept or procedure discussed. No part of this publication may be presented, copied or reproduced in any form or by any means without prior written permission of the Horticultural Development Company. The results and conclusions in this report are based on an investigation conducted over a two-year period. The conditions under which the experiments were carried out and the results have been reported in detail and with accuracy. However, because of the biological nature of the work it must be borne in mind that different circumstances and conditions could produce different results. Therefore, care must be taken with interpretation of the results, especially if they are used as the basis for commercial product recommendations. 2010 Agriculture and Horticulture Development Board AUTHENTICATION We declare that this work was done under our supervision according to the procedures described herein and that the report represents a true and accurate record of the results obtained. Robert Irving Consultant ADAS UK Ltd Signature ....R I Irving Date...8 February 2011 Report authorised by: T M O’Neill Horticulture Research Manager ADAS UK Ltd Signature.. p.p. Dr Kim Green Date...14 February 2011 2010 Agriculture and Horticulture Development Board CONTENTS Grower Summary ............................................................................................................ 1 Headline ...................................................................................................................... 1 Background and expected deliverables ....................................................................... 1 Summary of the project and main conclusions ............................................................ 1 Financial benefits ........................................................................................................ 4 Action points for growers ............................................................................................. 5 Science Section .............................................................................................................. 6 Introduction ................................................................................................................. 6 Materials and methods ................................................................................................ 8 Results ...................................................................................................................... 14 Discussion................................................................................................................. 22 Project Conclusions .................................................................................................. 24 Technology transfer .................................................................................................. 25 2010 Agriculture and Horticulture Development Board GROWER SUMMARY Headline Four treatments (1-Osmocote, 2-Plantmate Granules, 7-Omex Bio and 9-Feed Solution) applied at planting improved early canopy development in one of two everbearer strawberry varieties in 2010 but did not result in yield benefits. Background and expected deliverables Most of the UK strawberry crop is still produced in plastic-covered raised beds in field soils. The soil is commonly fumigated and there is a strong desire to prolong the value of this investment into a second crop by replanting the beds. This saves costs and reduces the use of soil fumigants. Good plant establishment has a significant effect on yield. Strawberry growers are increasingly considering whether to continue to produce in field soil or switch to substrate systems. Replanting of beds has become more problematic as a farm's soil is repeatedly used for strawberries. Specific objectives of this project are: To indentify non-pesticide products that improve plant establishment of replanted everbearer crops into previously cropped soil. To compare the performance of these products with standard agronomic treatments. To quantify the establishment treatments on initial canopy development and on yield in July, August and September. To ascertain the financial worthiness of the treatments applied. Summary of the project and main conclusions Products evaluated in the first year of the project in 2009 included Bio-Fungus Granules, Broadleaf P4, Broadleaf root dip, Humaroot SP, Omex Bio 18, Omex DP98, Plantmate Drench, Scotts Miracle Gro, side forking, Standard feed solution 1:1:1 and Vaminoc S. 2010 Agriculture and Horticulture Development Board 1 Bare root everbearer varieties were chosen to provide the most challenging plant material for establishment in 2009. In 2010, twelve products (Osmocote Exact Standard 5-6 month 15:9:12, Plantmate Granules, Broadleaf P4, Agralan Revive, Omex DP98, Huma-Root SP, Omex Bio 18, Side forking, Standard feed solution 1:1:1, Plantmate Drench, Vaminoc S, Radifarm) were selected following the results in 2009. Full details of all products applied in 2009 and 2010 are listed in Tables 1 and 2 (below). They were applied to potted strawberry varieties Eve´s Delight and Evie 2, planted into once-used beds in Derbyshire in March 2010. Potted plants were used in 2010, to improve uniformity of plant stock. Slightly heavier soil was also used in 2010 than had been used the previous year. Table 1: Details of product application rates for 2010 and 2009 Treatment Number Name 2010 application rates 2009 application rates 1 Osmocote Exact Standard 5-6 month 15:9:12 3 g in planting hole per plant Miracle Gro 18:9:10, 3 g granules in planting hole per plant 2 Plantmate Granules Not used 3 Broadleaf P4 4 Agralan Revive 5 Omex DP98 6 Huma-Root SP 7 Omex Bio 18 8 Side forking 9 Standard feed solution 1:1:1 1 g in planting hole per plant 1 g in planting hole per plant 10 ml per litre, 250 ml drench per plant 4 ml per litre of water, 250 ml drench per plant 0.4 g per litre, 250 ml drench per plant 6 ml per litre of water, 250 ml drench per plant One four pronged fork per planting hole 6 ml per litre of water, 250 ml drench per plant 10 Plantmate Drench 1.16 g per litre, 250 ml drench per plant 11 Vaminoc S 12 Radifarm 13 Control 8 g granules in planting hole per plant 2.5 ml /litre, 250 ml drench per plant No treatment Pre planting root dip at 10 g per litre of water for 1015 minutes 2 g granules in planting hole per plant Not used 1 g in planting hole per plant Not used 2 ml per litre of water, 250 ml per plant 0.1g per litre of water, 250 ml drench per plant 2 ml per litre of water, 250 ml per plant One four pronged fork per hole 3 ml per litre of water, 250 ml drench per plant No treatment 2010 Agriculture and Horticulture Development Board 2 Table 2: Details of product claims Treatment Number Name (Distributor/Manufacturer) Properties and product claims 1 Osmocote Exact Standard 5-6 month 15:9:12 (Scotts) Controlled release fertilisers slowly release fertiliser through a polymer coated granule. They are widely and successfully used for container ornamental production, where nutrition is placed in the compost for the life of the crop and only water is applied thereafter. Scotts have been developing their use for soil grown crops. They may reduce the amount of soil leached nutrients. 2 Plantmate Granules (FAST) A granular formulation containing proprietary strains of Trichoderma harzianum. It claims a protective quality against pathogens e.g. Pythium, Rhizoctonia and Phytophthora. Also a claim to stimulate auxins for better growth. 3 Broadleaf P4 (Agriculture Polymers International) Broadleaf P4 is a granular polymer formulation that absorbs and stores hundreds of times its own weight of water. This property claims to reduce losses to moisture stress and improves establishment and growth. Dissolved nutrients are also absorbed, though available to the crop, and may reduce leaching. 4 Agralan Revive (Agralan) A liquid culture of the bacterium Bacillus subtilis, which helps create soil conditions for healthy plant growth. Used for seedlings, cuttings and other plant material as a soil drench, a root dip, post planting treatment or compost additive. 5 Omex DP98 (Omex) A phosphite source of phosphorous that is normally applied as a foliar spray and sometimes as a drench at planting. As a foliar application, it is claimed to improve rooting and promote plant health. 6 Huma-Root SP (Plant Solutions) A humic and fulvic acid powder that is applied as a drench. Their high cation exchange capacity is claimed to enhance nutrient uptake, improved root growth and reduced transplant stress. 7 Omex Bio 18 (Omex) A liquid formulation of kelp, major and minor elements. It can be applied as a drench or foliar spray. The contained 'bio stimulants' and nutrients are claimed to produce improvements in root growth and nutrient uptake. 8 Side forking Poor soil structure is a major cause of failed plant establishment. Side forking a used bed can loosen the soil and may improve root development. A four pronged fork was inserted to 30 cm depth to the side of the replanting hole immediately after planting, then gently firmed back to ensure good root contact. 9 Standard feed solution 1:1:1 A basic feed of N:P:K. Vegetable transplant work in the eighties showed a yield benefit from nutrient solution drenching at planting. The application of a feed strength solution using straight feeds, coupled with the consolidating process of a drench may improve establishment in strawberry beds. 2010 Agriculture and Horticulture Development Board 3 Table 2 continued: Detail of product claims 10 Plantmate Drench (FAST) A wettable powder formulation containing proprietary strains of Trichoderma harzianum. It claims a protective quality against pathogens e.g. Pythium, Rhizoctonia and Phytophthora. It also claims to stimulate auxins for better growth. 11 Vaminoc S (Fargro) A non-soluble granular formulation containing arbuscular mycorrhizal fungi, which have been specially selected for strawberries. Fungal hyphae extend from the inoculated roots improving the absorptive surface area of root systems. This process claims more rapid crop establishment, disease protection and increased yields. 12 Radifarm (Hutchinsons) A liquid formulation of enzymatically produced extract from plant material with added natural components, which help stimulate the formation of new roots, and the extension of the existing season. 13 Control No treatment applied The key findings were as follows: No treatments significantly improved the yield for any variety or transplant type used in 2009 and 2010. In 2010, four treatments (1-Osmocote, 2-Plantmate Granules, 7-Omex Bio and 9-Feed Solution) significantly improved canopy development in Eve's Delight, although the improvements did not last for the whole growing season. The same treatments did not enhance canopy development in Evie 2. The lack of significant growth improvements following treatment applications to different varieties and transplant materials make it hard to recommend these root treatments for conventionally grown everbearer strawberries planted in a healthy well managed soil. It is possible that treatment differences might have been more marked under more adverse soil conditions or had sequential applications been used. Financial benefits No treatment showed a lasting significant improvement of growth or yield over the control in 2010. This is despite increasing the application rates of the more promising treatments from the 2009 trial. There are no financial benefits demonstrated in this trial. 2010 Agriculture and Horticulture Development Board 4 Action points for growers Growers suffering from poor crop performance on replanted beds cannot look to root treatments applied in the manner of this trial to provide a significant improvement. Given these results, the options are: Evaluate efficacy of past fumigant use and areas for improvement Acquire fresh ground Examine the costs/benefits of a move to soilless substrates 2010 Agriculture and Horticulture Development Board 5 SCIENCE SECTION Introduction Everbearer strawberry varieties respond positively to rapid establishment right from the start. Establishment rates are presently improved with fleecing at planting and early tunnelling. Both practices lift the soil temperature early in the season and improve the speed of root development and establishment. This trial reviewed treatments that may improve this process further. Bare root material was chosen for the first year as it may better express the benefits of enhanced root zone development compared to potted material (see Year 1 Annual report). Potted material was used in 2010 to reflect industry practice and provide more even sized planting material. The objectives of the experiment in 2010 were to maximise any potential improvements found with the 2009 treatments and to quantify any growth improvements by means of early canopy expansion, then later yield measurement. The 2009 results showed a canopy improvement for some products, although these were not statistically significant. Their application rates were increased for 2010 after discussion with the manufacturers and HDC. Three new products were added, two of which had new activities. Two were dropped. These changes were made to maximise the chances of identifying a useful response. Table 3: Treatments Unchanged For 2010 Trial Treatment Number Name Reasoning 3 Broadleaf P4 No change to 2009 8 Side forking No change to 2009 13 Control No change to 2009 2010 Agriculture and Horticulture Development Board 6 Treatments adjusted for 2010 trial Treatment Number Name Reasoning 1 Osmocote Exact Standard 5-6 month 15:9:12 There was a sample delivery problem in 2009. Miracle Gro 18:9:10 was replaced by the intended equivalent professional brand for 2010 5 Omex DP98 Rate increased to seek a better response in 2010 6 Huma-Root SP Rate increased to seek a better response in 2010 7 Omex Bio 18 Rate increased to seek a better response in 2010 9 Standard feed solution 1:1:1 Rate increased to seek a better response in 2010 10 Plantmate Drench A root drench was advised by the suppliers for the 2010 trial rather than the 2009 advice to root dip 11 Vaminoc S Rate increased to seek a better response in 2010 Treatments added to 2010 trial Treatment Number Name Reasoning 2 Plantmate Granules Plantmate Granules were not available in 2009 4 Agralan Revive Agralan Revive was not available in 2009, and offered a unique activity for 2010 12 Radifarm Radifarm was not available in 2009, and offered a unique activity for 2010 2010 Agriculture and Horticulture Development Board 7 Treatments dropped from 2010 trial Treatment Number Name Reasoning - Bio Fungus Granules No longer on sale for 2010 and caused transient root burn in 2009 Broadleaf Root Dip No advantage noted in 2009. There was little water absorbing capacity compared to the hole application. Removed to make space for additional treatments in 2010 - Materials and methods Site details A second year raised bed crop was chosen at JW Busby & Partners, Chilcote, Derbyshire. The soil was a well graded, free draining sandy loam, pH 6.6 with no obvious signs of compaction or root disease and, as such, had excellent potential for good establishment. The 2009 season had however been cropped with the highly wiltsensitive variety Sweet Eve, which suffered low level verticillium wilt, following soil disinfestation the previous autumn. The dead Sweet Eve crowns were pulled out immediately before replanting. The trial was planted within a large block of replanted Evie 2, which remained disease free for 2010. All of the 2010 plants were planted directly into the previous plant holes. Soil samples for nutrient analysis were taken as a zigzag pattern on 2nd March 2010 and analysed for major nutrients and texture. Treatments Thirteen root zone treatments including the control were administered at planting (Tables 4 and 5). Normal crop management practices, including trickle feeding using a conventional feed, were applied thereafter. 2010 Agriculture and Horticulture Development Board 8 Table 4: Details of product application rates for 2010 and 2009 Treatment Number Name 2010 application rates 2009 application rates 1 Osmocote Exact Standard 5-6 month 15:9:12 3 g per plant in planting hole Miracle Gro 18:9:10, 3 g granules per plant in planting hole 2 Plantmate Granules 1 g in planting hole per plant Not used 3 Broadleaf P4 1 g per plant in planting hole 1 g per plant in planting hole 4 Agralan Revive 10 ml per litre, 250 ml drench per plant Not used 5 Omex DP98 4 ml per litre of water, 250 ml drench per plant 2 ml per litre of water, 250 ml per plant 6 Huma-Root SP 0.4 g per litre, 250 ml drench per plant 0.1g per litre of water, 250 ml drench per plant 7 Omex Bio 18 6 ml per litre of water, 250 ml drench per plant 2 ml per litre of water, 250 ml per plant 8 Side forking One four pronged fork per planting hole One four pronged fork per hole 9 Standard feed solution 1:1:1 6 ml per litre of water, 250 ml drench per plant 3 ml per litre of water, 250 ml drench per plant 10 Plantmate Drench 1.16 g per litre, 250 ml drench per plant Pre planting root dip at 10 g per litre of water for 1015 minutes 11 Vaminoc S 8 g granules per plant in planting hole 2 g granules per plant in planting hole 12 Radifarm 2.5 ml /litre, 250 ml drench per plant Not used 13 Control No treatment No treatment 2010 Agriculture and Horticulture Development Board 9 Table 5: Detail of product claims Treatment Number 1 Name (Distributor/Manufacturer) Osmocote Exact Standard 5-6 month 15:9:12 (Scotts) 2 Plantmate Granules (FAST) 3 Broadleaf P4 (Agriculture Polymers International) 4 Agralan Revive (Agralan) 5 Omex DP98 (Omex) 6 Huma-Root SP (Plant Solutions) 7 Omex Bio 18 (Omex) 8 Side forking Properties and product claims Controlled release fertiliser slowly releases nutrients through a polymer coated granule. They are widely and successfully used for container ornamental production, where nutrition is placed in the compost for the life of the crop and only water is applied thereafter. Scotts have been developing their use for soil grown crops. They may reduce the amount of soil leached nutrients. A granular formulation containing proprietary strains of Trichoderma harzianum. It claims a protective quality against pathogens e.g. Pythium spp, Rhizoctonia spp and Phytophthora spp. Also a claim to stimulate auxins for better growth. Broadleaf P4 is a granular polymer formulation that absorbs and stores hundreds of times its own weight of water. This property claims to reduce losses to moisture stress and improves establishment and growth. Dissolved nutrients are also absorbed, though available to the crop, and may reduce leaching. A liquid culture of the bacterium Bacillus subtilis, which helps create soil conditions favourable to healthy plant growth. For seedlings, cuttings and other plant material as a soil drench, a root dip, post planting treatment or compost additive. A phosphite source of phosphorous that is normally applied as a foliar spray. Sometimes as a drench at planting. There is claimed improved rooting and promoted plant health as a foliar application. A humic and fulvic acid powder that is applied as a drench. The high cation exchange capacity is claimed to enhance nutrient uptake, improved root growth and reduced transplant stress. A liquid formulation of kelp, major and minor elements. It can be applied as a drench or foliar spray. The contained 'bio stimulants' and nutrients are claimed to produce improvements in root growth and nutrient uptake. Poor soil structure is a major cause of failed plant establishment. Side forking a used bed can loosen the soil and may improve root development. A four pronged fork was sunk 30 cm deep to the side of the replanting hole immediately after planting, then gently firmed back to ensure good root contact. 2010 Agriculture and Horticulture Development Board 10 Table 5 continued: Detail of product claims 9 Standard feed solution 1:1:1 10 Plantmate Drench (FAST) 11 Vaminoc S (Fargro) 12 Radifarm (Hutchinsons) 13 Control A basic feed of N:P:K. Vegetable transplant work in the eighties showed a yield benefit from nutrient solution drenching at planting. The application of a feed strength solution using straights, coupled with the consolidating process of a drench may improve establishment in strawberry beds. A wettable powder formulation containing proprietary strains of Trichoderma harzianum. It claims a protective quality against pathogens e.g. Pythium spp, Rhizoctonia spp and Phytophthora spp. Also a claim to stimulate auxins for better growth. A non-soluble granular formulation containing arbuscular mycorrhizal fungi, which have been specially selected for strawberries. Fungal hyphae extend from the inoculated roots improving the absorptive surface area of root systems. This process claims more rapid crop establishment, disease protection and increased yields. A liquid formulation of enzymatically produced extract from plant material with added natural components, which help stimulate the formation of new roots, and the extension of the existing season. No treatment applied Soil compaction assessment An analogue soil compaction gauge (manufacturers Spectrum Technologies Inc) was used to assess bed compaction after removal of the previous crop but before replanting on the same day. Ten readings were taken in a zigzag pattern across the site for each variety. Planting material 7 cm potted everbearers varieties Evie 2 and Eve´s Delight were used. These were very uniformly sized plants, an improvement on the 2009 bare root plants. Crop management and treatment application The crop followed the conventional cropping pattern for the farm. The soil beds were prepared, sterilised with metham sodium (Basamid) in autumn 2008, and then cropped in 2009 with Sweet Eve. Plants were killed after harvest with glyphosate and the dead plants removed just before replanting in 2010. 2010 Agriculture and Horticulture Development Board 11 Treatments were applied (Figs 2 and 3) and the crops planted on 2nd March 2010. Granule treatments were placed in the hole before planting and drenches immediately after planting (Figs 2 & 3). The area was covered with single layer fleece on 4th March and tunnelled on 17th April. The fleece was removed on 15th May. Fig 2: Photo of granules in hole Fig 3: Drenching a hole Crop assessment Two parameters were chosen, canopy development and picked yield. It was considered that measuring root volumes would be excessively time consuming for this project and possibly inaccurate. Canopy development was measured as groundcover within the first three months before there was overlapping of plant canopies. The crop canopy area was recorded as a digital image using a fixed height tripod to ensure a standardised recorded area. The images were later scored manually using a standard overlaid grid. Four recordings were made to provide three sets of canopy expansion data. Each plot was photographed and scored separately. The canopy score is the proportion of squares filled per plot (Fig 1). Each plant was scored each time then totalled for the plot. The canopy score was not converted into m2 of canopy as this contributed nothing to the assessment. It was calculated as the increase in filled squares since the previous recording. 2010 Agriculture and Horticulture Development Board 12 Fig 1: photo of grid over canopies The results are presented as grid scores per treatment. Picked yield was established as three picks per plot to reflect a week’s picking during early August, late August and mid September. This was a total of nine picks. Picking dates were: August 2nd, 5th & 9th August 23rd, August 26th, August 30th Sept 14th, Sept 17th, Sept 22nd This was not a total harvest of the season but provided a second check for the canopy data to identify if there were significant advantages from any particular treatment in the main yielding times of the season. Each plot was weighed separately and recorded by carefully selected and trained farm staff. The results are presented as a combined weight for the four replicates. Experiment design and analysis The two varieties Evie 2 and Eve´s Delight were planted as two adjacent randomised complete block trials. Each trial contained four adjacent beds with a full replication of the 13 treatments in each bed. Individual plots contained eight plants plus two guard plants, approximately 1.6 m of bed length. The trial was planted at 5 plants per metre of bed on a standard double row; the field density was 29,411 plants per ha. 2010 Agriculture and Horticulture Development Board 13 An Analysis of Variance (ANOVA, using Genstat) was applied to the data to identify statistically significant treatment differences. Results Soil nutrient analysis and texture Both blocks had closely matched nutrient status. The two blocks also had closely matched textures (Tables 6 and 7). Table 6: Soil nutrient status, 2nd March 2010 Index mg/l (Available) Soil pH P K Mg P K Mg Eve's Delight 6.6 4 3 3 47.2 313 168 Evie 2 6.6 3 3 4 42.8 307 179 Table 7: Soil texture Eve's Delight Evie 2 Sand (2.00 – 0.06mm) % 47 48 Silt (0.060 - 0.002mm) % 25 23 Clay (< 0.002mm) % 28 29 Textural Classification Clay Loam Clay Loam Soil compaction status The soil was moist and easy to penetrate. Soil within a range of 0-200 PSI (0-1380 KN/m² = K Pa) is regarded as free of compaction problems. There was minimal compaction, the lower the number, the less resistance to the meter tip (Table 3). Many of the higher scores were due to a stone being in the way. No areas of compaction or poor drainage were identified in the trial site. These were well constructed beds in their second year. 2010 Agriculture and Horticulture Development Board 14 Table 8: Soil compaction readings pre-planting – 2nd March 2010 PSI (KN/m² = K Pa) at 0 cm depth PSI (KN/m² = K Pa) at 10 cm depth PSI (KN/m² = K Pa) at 20 cm depth PSI (KN/m² = K Pa) at 30 cm depth Eve's Delight 0 (0) 10 (69) 10 (69) 20 (138) 0 (0) 0 (0) 10 (69) 25 (173) 0 (0) 15 (104) 15 (104) 20 (138) 0 (0) 0 (0) 10 (69) 40 (276) 0 (0) 0 (0) 10 (69) 40 (276) 0 (0) 0 (0) 20 (138) 30 (207) 0 (0) 10 (69) 10 (69) 80 (552) 0 (0) 10 (69) 10 (69) 50 (346) 0 (0) 10 (69) 10 (69) 30 (207) 0 (0) 20 (138) 20 (138) 40 (276) 0 (0) 0 (0) 0 (0) 100 (692) 0 (0) 0 (0) 25 (173) 25 (173) 0 (0) 0 (0) 25 (173) 25 (173) 0 (0) 10 (69) 0 (0) 0 (0) 0 (0) 20 (138) 0 (0) 10 (69) 0 (0) 10 (69) 10 (69) 50 (346) 10 (69) 10 (69) 20 (138) 30 (207) 0 (0) 0 (0) 20 (138) 30 (207) 0 (0) 0 (0) 15 (104) 20 (138) 0 (0) 10 (69) 20 (138) 50 (346) Evie 2 Results summarising the effects of treatments on canopy development and yields in both strawberry varieties are shown in Tables 9 - 14. 2010 Agriculture and Horticulture Development Board 15 Table 9: Eve's Delight Canopy Scores Eve's Delight Canopy Score April 8th Canopy Score 10 8 6 4 2 0 1 2 3 4 5 6 7 8 9 10 11 12 13 11 12 13 Treatment LSD (At 5% level) 1.452, F pr 0.005 Eve's Delight Canopy Score April 16th 14 Canopy Score 12 10 8 6 4 2 0 1 2 3 4 5 6 7 8 9 10 Treatment LSD (At 5% level) 1.702, F pr < 0.001 Eve's Delight Canopy Score May 9th Canopy Score 32 24 16 8 Treatments 0 1 2 3 4 5 6 7 8 9 10 11 12 13 Treatment LSD (At 5% level) 5.539, F pr 0.005 1 Osmocote Exact Standard 5-6 month 15:9:12 2 Plantmate Granules 3 Broadleaf P4 4 Agralan Revive Eve's Delight Canopy Score May 20th 5 Omex DP98 6 Huma-Root SP Canopy Score 50 7 Omex Bio 18 40 8 Side forking 30 9 Standard feed solution 1:1:1 20 10 Plantmate Drench 10 11 Vaminoc S 0 1 2 3 4 5 6 7 8 Treatment 9 10 11 12 13 12 Radifarm 13 Control LSD (At 5% level) 7.699, F pr 0.048 2010 Agriculture and Horticulture Development Board 16 Table 10: Evie 2 Canopy Scores Canopy Score Evie 2 Canopy Score April 8th 6 4 2 0 1 2 3 4 5 6 7 8 9 10 11 12 13 11 12 13 Treatment LSD (At 5% level) 1.468, F pr 0.990 Evie 2 Canopy Score April 16th Canopy Score 10 8 6 4 2 0 1 2 3 4 5 6 7 8 9 10 Treatment LSD (At 5% level) 1.473, F pr 0.518 Canopy Score Evie 2 Canopy Score May 9th 30 20 10 0 1 2 3 4 5 6 7 8 9 10 11 12 13 Treatments 1 Osmocote Exact Standard 5-6 month 15:9:12 2 Plantmate Granules Treatment LSD (At 5% level) 5.115, F pr 0.055 3 Broadleaf P4 4 Agralan Revive Evie 2 Canopy Score May 20th 5 Omex DP98 6 Huma-Root SP Canopy Score 40 7 Omex Bio 18 30 8 Side forking 20 9 Standard feed solution 1:1:1 10 10 Plantmate Drench 0 11 Vaminoc S 1 2 3 4 5 6 7 8 Treatment 9 10 11 12 13 12 Radifarm 13 Control LSD (At 5% level) 9.256, F pr 0.384 2010 Agriculture and Horticulture Development Board 17 Table 11: Eve's Delight Change in Canopy Eve's Delight Change in Canopy Score April 8th to 16th Canopy Score 5 4 3 2 1 0 1 2 3 4 5 6 7 8 9 10 11 12 13 Treatment LSD (At 5% level) 1.062, F pr 0.033 Eve's Delight Change in Canopy Score April 16th to May 9th Canopy Score 20 15 10 5 0 1 2 3 4 5 6 7 8 9 10 11 12 13 Treatments Treatment 1 Osmocote Exact Standard 5-6 month 15:9:12 2 Plantmate Granules LSD (At 5% level) 4.808, F pr 0.024 3 Broadleaf P4 4 Agralan Revive Eve's Delight Change in Canopy Score May 9th to 20th 5 Omex DP98 Canopy Score 6 Huma-Root SP 20 7 Omex Bio 18 15 8 Side forking 10 9 Standard feed solution 1:1:1 10 Plantmate Drench 5 11 Vaminoc S 0 1 2 3 4 5 6 7 8 Treatment 9 10 11 12 13 12 Radifarm 13 Control LSD (At 5% level) 4.755, F pr 0.487 2010 Agriculture and Horticulture Development Board 18 Table 12: EvIe 2 Change in Canopy Evie 2 Change in Canopy Score April 8th to 16th Canopy Score 5 4 3 2 1 0 1 2 3 4 5 6 7 8 9 10 11 12 13 Treatment LSD (At 5% level) 1.125, F pr 0.320 Evie 2 Change in Canopy Score April 16th to May 9th Canopy Score 20 15 10 5 0 1 2 3 4 5 6 7 8 9 10 11 12 13 Treatment Treatments 1 Osmocote Exact Standard 5-6 month 15:9:12 2 Plantmate Granules LSD (At 5% level) 4.618, F pr 0.041 3 Broadleaf P4 Evie 2 Change in Canopy Score May 9th to 20th 4 Agralan Revive 5 Omex DP98 Canopy Score 20 6 Huma-Root SP 15 7 Omex Bio 18 10 8 Side forking 9 Standard feed solution 1:1:1 5 10 Plantmate Drench 0 1 2 3 4 5 6 7 8 9 10 11 12 13 Treatment 11 Vaminoc S 12 Radifarm 13 Control LSD (At 5% level) 6.328, F pr 0.621 2010 Agriculture and Horticulture Development Board 19 Table 13: Fruit weights and number, Eve’s Delight Eve's Delight Total Weight of Nine Picks Weight (g) 2500 2000 1500 1000 1 2 3 4 5 6 7 8 9 10 11 12 13 12 13 Treatment LSD (At 5% level) 576.3, F pr 0.152 Eve's Delight Berry Number of Nine Picks Berry number 120 90 60 30 1 2 3 4 5 6 7 8 9 10 11 Treatment LSD (At 5% level) 26.35, F pr 0.111 Treatments 1 Osmocote Exact Standard 5-6 month 15:9:12 2 Plantmate Granules Average berry weight (g) Eve's Delight Average Berry Weight (g)of Nine Picks 3 Broadleaf P4 4 Agralan Revive 21 5 Omex DP98 20 6 Huma-Root SP 19 7 Omex Bio 18 18 8 Side forking 9 Standard feed solution 1:1:1 17 10 Plantmate Drench 16 1 2 3 4 5 6 7 8 Treatment 9 10 11 12 13 11 Vaminoc S 12 Radifarm 13 Control LSD (At 5% level) 2.429, F pr 0.671 2010 Agriculture and Horticulture Development Board 20 Table 14: Fruit weights and number, Evie 2 Evie 2 Total Weight of Nine Picks Weight (g) 3000 2500 2000 1500 1 2 3 4 5 6 7 8 9 10 11 12 13 11 12 13 Treatment LSD (At 5% level) 754.3, F pr 0.254 Evie 2 Berry Number of Nine Picks Berry number 160 140 120 100 80 1 2 3 4 5 6 7 8 9 10 Treatment Treatments LSD (At 5% level) 38.08, F pr 0.402 1 Osmocote Exact Standard 5-6 month 15:9:12 2 Plantmate Granules Evie 2 Average Berry Weight (g) of Nine Picks 3 Broadleaf P4 4 Agralan Revive Average berry weight (g) 20 5 Omex DP98 6 Huma-Root SP 19 7 Omex Bio 18 18 8 Side forking 9 Standard feed solution 1:1:1 17 10 Plantmate Drench 11 Vaminoc S 16 1 2 3 4 5 6 7 8 Treatment 9 10 11 12 13 12 Radifarm 13 Control LSD (At 5% level) 1.854, F pr 0.092 2010 Agriculture and Horticulture Development Board 21 Discussion The 2010 potted plants had much greater size uniformity than the 2009 plants. It was hoped that any treatment effect on canopy development would then be visually noticeable on the plots. This was not so, the differences were small and only identified after measurements and statistical procedures. Each plot had eight plants, which were recorded individually for canopy size. Analysis of this data showed that size variability was uniform across all treatments. Four replicates and randomisation of plots gave confidence in the data. There was a marked difference in variety response. Eve's Delight: Assessment of Canopy and Yield Data The canopy scores all have a Frequency of Probability of <0.05, which indicates there are significant effects in the data, Table 9. Treatments 1 (Osmocote), 2 (Plantmate Granules), 7 (Omex Bio) and 9 (Feed Solution) all showed a consistent improvement over the Control for canopy scores. This improvement was usually statistically significant (P<0.05) for these treatments. There is a sign that these treatment benefits declined later in the season, since May 20th scores were not significantly better that the control. This may have been due to treatment breakdown over time, displacement by irrigation or increased beneficial factors such as soil temperatures increasingly influencing root development. The canopy differences, which give a measure of canopy expansion, also show a significant effect, though only for the first early assessment of April 8 to April 16 Although there was a trend for some treatments to apparently increase yield, no treatments showed a statistically significant improvement in yield compared with the untreated control for 2010, Table 10. There was a non-significant trend for treatment 7 (OmexBio) that contributed to early canopy expansion, to give yields that were lower than the untreated control. 2010 Agriculture and Horticulture Development Board 22 Evie 2: Assessment of Canopy and Yield Data No treatments showed a statistically significant improvement in canopy size or yield in 2010, Tables10, 12 and 14. Evie 2 is a tougher, more soil-borne disease resistant variety so may not have benefitted from these treatments in the same way as the more delicate Eve’s Delight. From June onwards, canopy measurement became increasingly meaningless as the canopy overlap between plants and within each plant increased. The 2009 results (Year 1 Annual Report) showed that treatments with a nutritional mode of action came closest to producing significant responses. This seems to still be the case again for 2010 where three of the four significant responses were nutrient based. Treatments 1 (Osmocote), 7 (Omex Bio) and 9 (Feed Solution) all showed a consistent improvement over the Control for canopy scores, although this was just for one variety, and did not translate into a yield response. There was an important turn around for the slow release fertiliser treatment. Two very similar brands of slow release fertiliser were used, one for each year. The 2009 brand ´Miracle Gro 18:9:10´ was placed in the planting hole and caused transient root scorch but had no long term deleterious effect. Slow release fertilisers have well proven advantages so treatment was included again for 2010, but managed differently. The 2010 equivalent ´Osmocote Exact Standard 5-6 month 15:9:12´ (treatment 1) was not at all damaging and was one of the treatments that showed a significant advantage on Eve´s Delight. It was placed 5 cm away from the planting hole and located on the trickle tape side. This avoided a damaging fertiliser hotspot near the developing roots. Overall, the product claims were for a range of properties that may not have been particularly relevant for this particular site. For example, not all the products recommended a single application at planting. They may have had foliar or trickle application for several applications but this was beyond the scope of this trial. The 2010 site had excellent clay loam soil for trouble free planting, with no serious history of major soil-borne pathogens. There was a very low level of loss to verticillium wilt with sensitive Sweet Eve in the previous year. No losses were seen in 2010. The 2010 Agriculture and Horticulture Development Board 23 soil also had a moderate pH and adequate phosphate index. It is possible that the soil could have been too fertile for the products to express their properties. Possibly a root promoting treatment providing phosphorous would have little additional impact under these conditions. Similarly, side forking and treatment with products claiming to manage soil-borne pathogens had minimal impact under conditions of good soil structure with low levels of pathogen inoculum. Two products, Huma-Root SP and Omex Bio 18, actually state that the greatest effects would be seen under adverse conditions. Project Conclusions In 2009 and 2010, a range of treatments applied at planting was evaluated for their effect on everbearer strawberry establishment, growth and yield. No treatments improved the yield for any variety used (Camarillo, Albion, Eve's Delight and Evie 2) or transplant type (bare root material and potted material) for 2009 and 2010. Significantly improved canopy effects were noted on one variety only (Eve's Delight) in 2010 following treatment with Osmocote, Plantmate Granules, Omex Bio and Feed Solution, although these effects were short-lived. Controlled release fertiliser brand Miracle Gro 18:9:10 caused temporary leaf scorch in 2009, though this did not affect yield. Osmocote Exact Standard 5-6 month 15:9:12, was included in 2010 under revised manufacturer guidance to place it between the planting hole and trickle line. This avoided root scorch and no leaf scorching was noted either, due to better placement. These findings make it hard to recommend these root treatments as a single planting application for conventionally grown everbearer strawberries planted in healthy well managed soil. It is possible that treatment differences might have been more marked under more adverse soil conditions. From the results of this project, growers suffering poor crop performance on replanted beds cannot rely on the single application root treatments tested in this trial to provide a significant improvement. Sequential applications may improve crop responses, but this not tested in this trial. 2010 Agriculture and Horticulture Development Board 24 Given these results, the alternative options are to evaluate the efficacy of past sterilant use and areas for improvement, to acquire fresh ground or to undertake a financial cost/benefit appraisal of a move to substrate production. Technology transfer HDC News, December 2009, ‘Get the Best from Re-used Beds’, Issue 159, pp20-21. HDC News, February 2011, ‘Healthy Start for a Second Crop', Issue 170, p16 2010 Agriculture and Horticulture Development Board 25