BIOL 120 W13 Instructor: D. Rintala Bryant & Stratton College NAME_______________________________________________________ DATE_____________ BIOL 120 Exam #1 Do not write in this box. True/ False= _____/10 Multiple Choice= _____/70 Short Answer= _____/20 Total Points= _____/100 Letter Grade= _____ True/ False. 1 points each, select A for true, B for false. 1. During a scientific experiment, the control group is used to directly test or measure the consequences of a variable in the study. 2. If during the gram stain procedure, the bacterial cells were viewed immediately after crystal violet was applied, gram-positive cells would be purple but gram-negative cells would be colorless. 3. Viruses cause most human cancers. 4. Mycoplasmas are named for the large quantities of mycolic acid in their cells walls. 5. The term diplococci refers to an irregular cluster of spherical bacterial cells. 6. All algae have chloroplasts. 7. Prophages can be activated into viral replication and enter the lytic cycle. 8. Viral infections are easier to treat with drugs than bacterial infections. 9. In the levels of hierarchy, family comes before class. 10. Diseases of animals caused by fungi are called mycoses. BIOL 120 W13 Instructor: D. Rintala Bryant & Stratton College NAME_______________________________________________________ DATE_____________ Multiple choice. Select the best answer from the choices below. Mark the answer on the answer sheet provided. Each multiple choice question is 2 points. (35 questions, 70 points total) 11. The transfer of drug resistance factors from one bacterium to another bacterium is the result of DNA transfer through a hollow tubular structure known as a. Cilia b. Conjugation pilus c. Fimbriae d. Flagellum 12. This is the system of arranging and naming organisms: a. Hierarchy b. Nomenclature c. Taxonomy d. Identification 13. Bacterial cells may or may not have extrachromosomal DNA, which is located in their _________. a. Nucleus b. Mitochondria c. Plasmid d. Cell wall 14. You examine a Gram-stained slide prepared from a swab obtained from an 8 year-old boy complaining of a sore throat. You see purple chains of spherical bacteria. You recognize these bacteria as: a. Gram positive staphylococci b. Gram positive streptobacilli c. Gram positive streptococci d. Gram negative streptococci BIOL 120 W13 Instructor: D. Rintala Bryant & Stratton College NAME_______________________________________________________ DATE_____________ 15. In the scientific method, scientists formulate a _______________________, which is a tentative explanation to account for what they have observed or measured, and then conduct experiments to determine its validity. a. Fact b. Hypothesis c. Theory d. Law 16. Which of the following follows the rules of binomial nomenclature? a. Escherichia coli b. e.coli c. Escherichia c. d. Escherichia coli 17. Which of the following is true of endospores? a. Endospores are a reproductive structure. b. Bacteroides and E.coli are genera that produce them. c. Endospores are dormant. d. Endospores are susceptible to boiling and chemicals. 18. Following binomial nomenclature, which of the following makes up the scientific name of an organism? a. The genus name and then the species name b. The family name and then the genus name c. The genus name and then the order name d. The species name and then the genus name 19. The major groups of organisms studied in microbiology are: a. bacteria, viruses, protozoa, algae and fungi. b. viruses, plants, mushrooms and protozoa. c. fungi, animals, plants and bacteria. d. viruses, plants, algae, bacteria and protozoa. BIOL 120 W13 Instructor: D. Rintala Bryant & Stratton College NAME_______________________________________________________ DATE_____________ 20. Disease causing microorganisms are called a. Eukaryotes b. Decomposers c. Pathogens d. Prokaryotes 21. Respiratory enzymes of prokaryotic cells are located within the _______________. a. Mitochondria b. Cell membrane c. Glycocalyx d. Plasmid 22. The general structure of the phospholipid component of the phospholipid bilayer consists of a __________ head and a _____________ tail. a. Hydrophilic, long b. Hydrophobic, hydrophilic c. Hydrophilic, hydrophobic d. Long, hydrophilic 23. Gram negative prokaryotic cells have a _______ peptidoglycan layer, whereas grampositive prokaryotic cells have a __________ peptidoglycan layer with their cell wall. a. Thin, thick b. Thick, thin c. Thin, non-existent d. Thick, thick 24. ____________ is an asexual method of reproduction associated with yeasts. a. Binary fission b. Mitosis c. Meiosis d. Budding 25. A flexible, spiral-shaped bacterium is called a a. spirochete. b. vibrio. c. coccobacillus. d. spirillum. BIOL 120 W13 Instructor: D. Rintala Bryant & Stratton College NAME_______________________________________________________ DATE_____________ 26. What is the active, feeding and reproducing morphological form of a protozoan? a. Cyst b. Endospore c. Trophozoite d. Schizogony 27. The 4 major characteristics a living organism has to have to be considered “living” are: a. Reproduction, cell wall, responsiveness, metabolism b. Mitochondria, chromosomes, cell wall, reproduction c. Responsiveness, metabolism, reproduction, growth d. Growth, responsiveness, metabolism, cell wall 28. The eukaryotic cell organelle that most resembles a bacterial cell is the a. Ribosome b. Nucleus c. Mitochondria d. Lysosome 29. In order to reproduce sexually, diploid cells must produce _________ gametes through _________. a. Triploid, mitosis b. Haploid, meiosis c. Haploid, mitosis d. Diploid, meiosis 30. Species of Mycobacterium have cell walls which contain up to 60% of what waxy substance? a. Glucose b. Lipid A c. Sterols d. Mycolic Acid 31. Creutzfeld- Jakob disease is a. A spongiform encephalopathy of humans. b. Also called mad cow disease. c. Caused by a viroid. d. Caused by a chronic latent virus. BIOL 120 W13 Instructor: D. Rintala Bryant & Stratton College NAME_______________________________________________________ DATE_____________ 32. Bacteria of the genus Mycoplasma are distinguished from other bacterial cells by a. unique ribosomes. b. the presence of mycolic acid in their cell walls. c. the absence of a cell wall. d. the absence of a cytoplasmic membrane. 33. Which of the following organelles is responsible for producing most of the ATP in a eukaryotic cell? a. Golgi apparatus b. mitochondrion c. smooth endoplasmic reticulum d. nucleus 34. Single-celled organisms called ciliates are a. fungi. b. protozoa. c. algae. d. bacteria. 35. Which of the following does not pertain to helminths? a. in kingdom Protista b. parasitic worms c. eggs and sperm for reproduction d. often alternate hosts in complex life cycles 36. Of the microscopic fungi, _________ are filamentous fungi made of long thread-like cells called hyphae. a. Yeast b. Mold c. Mushroom d. Flower 37. The correct sequence of events in viral multiplication is a. replication, penetration, maturation, assembly, absorption, release. b. assembly, maturation, replication, release, penetration, adsorption. c. adsorption, release, maturation, replication, assembly, penetration. d. adsorption, penetration, replication, maturation, assembly, release. BIOL 120 W13 Instructor: D. Rintala Bryant & Stratton College NAME_______________________________________________________ DATE_____________ 38. Viruses that cause infection resulting in alternating periods of activity with symptoms and inactivity without symptoms are called a. latent b. oncogenic. c. prions. d. viroids. 39. How do enveloped animal viruses exit their host? a. Budding or exocytosis b. Bursting the host cell c. Rupturing the virus d. Endocytosis 40. Clostridium botulinum is made virulent by incorporated prophage genes encoding for the botulinum toxin. What term describes this process? a. Lytic phase b. Adsorption c. Lysogenic conversion d. Latent phase 41. Viruses belong to which of the following Kingdoms? a. Protista b. Fungi c. Monera d. None of the choices are correct. 42. A positive (+) strand of RNA a. Cannot function directly as mRNA b. Makes a minus (-) strand of RNA which can then act as mRNA c. Can directly act as mRNA. d. Must be double stranded. 43. When a virus enters a lysogenic phase, it means a. the virus is integrated into the DNA of the host cell and is latent. b. the virus is bursting through the host cell membrane. c. the virus is starting biosynthesis of its nucleic acid. d. the virus will remain in circulation and not continue infecting its host. BIOL 120 W13 Instructor: D. Rintala Bryant & Stratton College NAME_______________________________________________________ DATE_____________ 44. The negative (-) sense strand of RNA a. Plays no role b. Makes RNA replicase c. Is used to synthesize more + strands d. Acts as mRNA to make viral proteins 45. Retroviruses have a. One copy of single-stranded RNA b. Two copies of single-stranded RNA c. One copy of double-stranded RNA d. Two copies of single-stranded DNA BIOL 120 W13 Instructor: D. Rintala Bryant & Stratton College NAME_______________________________________________________ DATE_____________ Short Answer: Answer each question in the spaces provided (20 points total). 46. Using the bacterial cell below, label the following: ribosomes, chromosome, flagellum, pilus, fimbriae, inclusion body, cell envelope, cell wall, slime layer, capsule. (5 points, ½ point for each structure) 47. This question will compare and contrast prokaryotes and eukaryotes. a. Give 1 example of a prokaryote. (1 point) b. Give 1 example of a eukaryote. (1 point) c. Give an example of a microbe that doesn’t fit into either category of prokaryote or eukaryote. (1 point) d. Describe 2 differences between prokaryotes and eukaryotes (2 points) BIOL 120 W13 Instructor: D. Rintala Bryant & Stratton College NAME_______________________________________________________ DATE_____________ 48. Gram-positive and Gram-negative bacteria vary in their cell envelope structures. A. Label the following structures (remember, some of these are found in both, some only in one or the other): Gram-positive, Gram-negative, cell wall, cell envelope, teichoic acid, plasma membrane, lipopolysaccharide, periplasmic space, peptidoglycan layer, outer membrane, porins. (5 points) 49. Draw a naked virus and an enveloped virus and label the following: capsid, envelope, spike, nucleic acid on one or both (if they have the structure). (5 points)