Nucleic acid binding study of surfactant copper(II) complex
containing dipyrido[3,2-a:2 '-3 '-c]phenazine ligand as an
intercalator: in vitro antitumor activity of complex in human
liver carcinoma (HepG2) cancer cells
By:Nagaraj, K (Nagaraj, Karuppiah)[ 1 ] ; Murugan, KS (Murugan, Krishnan
Senthil)[ 2 ] ; Thangamuniyandi, P (Thangamuniyandi, Pilavadi)[ 3 ] ; Sakthinathan, S (Sakthinathan,
Subramanian)[ 1 ]
RSC ADVANCES
Volume: 4
Issue: 99
Pages: 56084-56094
DOI: 10.1039/c4ra08049a
Published: 2014
View Journal Information
Abstract
A new surfactant copper(II) complex, [Cu(dppz)(2)DA](ClO4)(2), where dppz = dipyrido[3,2-a:2'-3'-c]
phenazine and DA-dodecylamine, has been synthesized and characterized by physico-chemical and
spectroscopic methods. The critical micelle concentration (CMC) value of this surfactant copper(II)
complex in aqueous solution was determined from conductance measurements. Specific conductivity
data at different temperatures was obtained for the evaluation of the temperature-dependent CMC
and the thermodynamics of micellization (Delta G(m)(0), Delta H-m(0) and Delta S-m(0)). The binding
interaction of this complex with nucleic acids (calf thymus DNA and yeast t-RNA) was investigated
using electronic absorption, fluorescence spectroscopy, viscometry, cyclic voltammetry (CV) and
thermal denaturation studies. In the presence of the nucleic acids, the UV-Vis spectrum of our
complex exhibited a redshift of the absorption band at 268 nm along with significant hypochromicity,
indicating intercalation of our complex with the nucleic acids. The intrinsic binding constant values are
K-b = 1.1 x 10(6) M-1 for DNA and 1.6 x10(6) M-1 for RNA. The viscosity measurements confirmed
that the complex-nucleic acid interaction occurs through intercalation. A competitive binding study
with ethidium bromide (EB) showed that the complex exhibits the ability to displace the nucleic acidbound EB, indicating that the complex binds to nucleic acids in strong competition with EB for the
intercalative binding site. CV results also confirmed this mode of binding. Some significant
thermodynamic parameters of the binding of the titled complex to DNA were also determined. The
antimicrobial and antifungal screening tests of this complex have shown good results. The copper(II)
complex exhibited pronounced activity against a human liver carcinoma (HepG2) cancer cell line.
Keywords
KeyWords Plus:CRITICAL MICELLE CONCENTRATION; DNA-BINDING; CRYSTALSTRUCTURE; RNA-BINDING; 1,10-
PHENANTHROLINE;RUTHENIUM(II); PHENANTHROLINE; CYTOTOXICITY; FLUORESCENCE; C
OBALT(III)
Author Information
Reprint Address: Nagaraj, K (reprint author)
Bharathidasan Univ, Sch Chem, Tiruchchirappalli 620024, Tamil Nadu, India.
Addresses:
[ 1 ] Bharathidasan Univ, Sch Chem, Tiruchchirappalli 620024, Tamil Nadu, India
[ 2 ] Vivekananda Coll, Post Grad & Res Dept Chem, Madurai 625234, Tamil Nadu, India
[ 3 ] Univ Madras, Dept Polymer Sci, Madras 600025, Tamil Nadu, India
E-mail Addresses:naturalnagaraj@gmail.com
Funding
Funding Agency
Grant Number
Bharathidasan University
CSIR
01(2461)/11/EMR-II)
DST
SR/S1/IC-13/2009
UGC
41-223/2012(SR)
View funding text
Publisher
ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD,
CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
Categories / Classification
Research Areas:Chemistry
Web of Science Categories:Chemistry, Multidisciplinary
Document Information
Document Type:Article
Language:English
Accession Number: WOS:000344997800047
ISSN: 2046-2069
Other Information
IDS Number: AT5QT
Cited References in Web of Science Core Collection: 61
Times Cited in Web of Science Core Collection: 0