Assistant professor, SSD 03/C1 born in Cosenza on October 12 th 1971
Address
University Magna Græcia of Catanzaro
Via S. Venuta, 88100 Catanzaro
Tel:+ 39 0961 369 4337 Email: gaspari@unicz.it
Cell: 333 7809558
Research interests
Miniaturization of analytical methods based on mass spectrometry for proteomic analysis.
Sample preparation for LC-MS (liquid chromatography-mass spectrometry) proteomic analysis of biological fluids.
Study of protein-protein interactions of interest in oncology.
Brief profile
I completed my undergraduate education at University of Calabria, in Organic Chemistry. I started to be interested in biological mass spectrometry during a six-month stage I undertake at TNO Pharma (NL), a
CRO devoted to applied research (1998). At the end of the stage, I was employed by the same company with a mixed employee/PhD student 5-year contract. In almost six years spent at TNO, I acquired a solid experience in biological mass spectrometry, and in coupling miniaturized separation methods such as nanoliquid chromatography and capillary electrophoresis to mass spectrometry for the analysis of biomolecules such as peptides. In 2004, I went back to Italy as post-doctoral fellow at the University of
Catanzaro (UMG), mainly working on a multidisciplinary project aiming at integrating nanotechnology with proteomics. My main task was to develop silicon-based platforms for the spectrometric analysis of biological samples. Besides, at UMG I have applied the knowledge I acquired in biological mass spectrometry to study the interaction partners of proteins of high bio-medical relevance using an innovative cross-linking approach, and to characterize post-translational modifications of proteins involved in tumorigenesis. As assistant professor (ricercatore) my main focus is to improve LC-MS-based proteomics methods for the proteomic analysis of biological fluids from the point of view of automation, sensitivity and quantitative precision.
Studies
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Diploma in Chemistry with Environmental Sciences (Erasmus programme, University of Kent, UK,
1993).
Master of Science in Chemistry, marks 110/110 with praise (University of Calabria, 1995).
PhD in Analytical Chemistry (University of Leiden, NL, 2006).

Work experiences
Assistant professor (ricercatore) in organic chemistry (University of Catanzaro, 2007-present).
Postdoctoral fellow assegno di ricerca - (University of Catanzaro, 2004-2007).
PhD student/part-time employee (TNO Pharma/University of Leiden, NL, 1999-2004).
Intern (TNO Pharma, NL, 1998).
Liutenant of the Italian Army –chemist- (1996-1997).
Teaching (University of Catanzaro)
Analytical Chemistry (8 CFU), M.Sc. in Pharmacy (2012-2014). Website: http://analiticaumg.weebly.com
General and Organic Chemistry (6+5 CFU), B.Sc. in Biotechnology (2011-2012).
Biomolecular Mass Spectrometry (2 CFU), M.Sc. in Biotechnology (2009-2010).
General and Organic Chemistry (5 CFU) B.Sc. in Food Science and Technology (2007-2010).
Additional information
“Abilitazione Scientifica” in Analytical Chemistry (2012).
International collaborations: A. Riccio, J. Timms, A. Saiardi (UCL, London, GB); M. Larsen (Odense,
DK); H. Raj (Chest Institute, Dehli, India); J. Krijgsfeld (Embl Heidelberg, DE); K. Verhoeckx (TNO
Zeist, NL).
Bibliometric data (Scopus): h-index = 19, total citations > 1000.
Relevant Publications (2006-2015)
[1] Bernaudo, F.; Monteleone, F.; Mesuraca, M.; Krishnan, S.; Chiarella, E.; Scicchitano, S.; Cuda, G.; Morrone,
G.; Bond, H. M.; Gaspari, M*. “Validation of a novel shotgun proteomic workflow for the discovery of protein-protein interactions : focus on ZNF521”, J. Proteome Res., 2015, in press.
[2] F. Pisani, T. Livermore, G. Rose, J. R. Chubb, M. Gaspari, and A. Saiardi, “Analysis of Dictyostelium discoideum inositol pyrophosphate metabolism by gel electrophoresis.,” PLoS One, vol. 9, no. 1, p. e85533,
Jan. 2014.
[3] A. Nicastri, M. Gaspari, R. Sacco, L. Elia, C. Gabriele, R. Romano, A. Rizzuto, and G. Cuda, “N-glycoprotein analysis discovers new up-regulated glycoproteins in colorectal cancer tissue.,” J. Proteome Res., vol. 13, no. 11, pp. 4932–41, Nov. 2014.
[4] A. Fabietti, M. Gaspari*, S. Krishnan, A. Quirino, M. C. Liberto, G. Cuda, and A. Foca, “Shotgun proteomic analysis of two Bartonella quintana strains,” Proteomics, vol. 13, no. 8, SI, pp. 1375–1378, Apr. 2013.
[5] C. Raso, C. Cosentino, M. Gaspari, N. Malara, X. Han, D. McClatchy, S. K. Park, M. Renne, N. Vadala, U. Prati,
G. Cuda, V. Mollace, F. Amato, and J. R. Yates III, “Characterization of Breast Cancer Interstitial Fluids by
TmT Labeling, LTQ-Orbitrap Velos Mass Spectrometry, and Pathway Analysis,” J. Proteome Res., vol. 11, no. 6, pp. 3199–3210, Jun. 2012.

[6] M. Di Sanzo, M. Gaspari, R. Misaggi, F. Romeo, L. Falbo, C. De Marco, V. Agosti, B. Quaresima, T. Barni, G.
Viglietto, M. R. Larsen, G. Cuda, F. Costanzo, and M. C. Faniello, “H-ferritin gene silencing in a human metastatic melanoma cell line: a proteomic analysis.,” J. Proteome Res., Nov. 2011.
[7] M. Gaspari* and G. Cuda, “Nano LC-MS/MS: a robust setup for proteomic analysis.,” Methods Mol. Biol., vol. 790, pp. 115–26, Jan. 2011.
[8] S. Krishnan, M. Gaspari*, A. Della Corte, P. Bianchi, M. Crescente, C. Cerletti, D. Torella, C. Indolfi, G. de
Gaetano, M. B. Donati, D. Rotilio, and G. Cuda, “OFFgel-based multidimensional LC-MS/MS approach to the cataloguing of the human platelet proteome for an interactomic profile.,” Electrophoresis, vol. 32, no. 6–7, pp. 686–95, Mar. 2011.
[9] A. de Laurentiis, M. Gaspari, C. Palmieri, C. Falcone, E. Iaccino, G. Fiume, O. Massa, M. Masullo, F. M.
Tuccillo, L. Roveda, U. Prati, O. Fierro, I. Cozzolino, G. Troncone, P. Tassone, G. Scala, and I. Quinto, “Mass spectrometry-based identification of the tumor antigen UN1 as the transmembrane CD43 sialoglycoprotein.,” Mol. Cell. Proteomics, vol. 10, no. 5, p. M111.007898, May 2011.
[10] F. Trapasso, F. Pichiorri, M. Gaspari, T. Palumbo, R. I. Aqeilan, E. Gaudio, H. Okumura, R. Iuliano, G. Di
Leva, M. Fabbri, D. E. Birk, C. Raso, K. Green-Church, L. G. Spagnoli, S. Venuta, K. Huebner, and C. M. Croce,
“Fhit interaction with ferredoxin reductase triggers generation of reactive oxygen species and apoptosis of cancer cells.,” J. Biol. Chem., vol. 283, no. 20, pp. 13736–44, May 2008.
[11] M. Gaspari*, K. C. M. Verhoeckx, E. R. Verheij, and J. van der Greef, “Integration of two-dimensional LC-MS with multivariate statistics for comparative analysis of proteomic samples.,” Anal. Chem., vol. 78, no. 7, pp.
2286–96, Apr. 2006.
[12] M. Gaspari*, M. Ming-Cheng Cheng, R. Terracciano, X. Liu, A. J. Nijdam, L. Vaccari, E. di Fabrizio, E. F.
Petricoin, L. A. Liotta, G. Cuda, S. Venuta, and M. Ferrari, “Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma.,” J. Proteome Res., vol. 5, no. 5, pp. 1261–6, May
2006.