Table S1: Lists of antibodies and TaqMan gene expression assays used in the studies. Table S1A lists the combinations of antibodies used along with
corresponding flourochrome conjugates, clone-names and suppliers. Table S1B lists the TaqMan Gene Expression Assays used in gene expression studies of
peripheral blood mononuclear cell (PBMC) subsets, cerebrospinal fluid (CSF) cells and PBMCs and whole blood.
Table S1A
Antigen presenting cells (APC)
T-cells
Antigen
CD1c (BDCA-1)
CD303 (BDCA-2)
CD19
CD14
Conjugate
PerCP-Cy5.5
FITC
PE-Cy7
Qdot 605
Clone
L161
AC144
HIB19
TüK4
Miltenyi
Biolegend
Invitrogen
APC Tube 1
CD209 (DC-SIGN)
CD83
CCR7
APC
PE
PB
9E9A8
HB15e
TG8/CCR7
Biolegend
Biolegend
Biolegend
APC Tube 2
CD38
CD138
CD27
APC
PE
V450
HIT2
MI15
M-T271
Biolegend
Biolegend
BD Biosciences
CD40
CD70
CD86
APC
PE
PB
HB14
Ki-24
IT2.2
Biolegend
BD Biosciences
Biolegend
APC Tube 4
CD275 (ICOSL)
CD252 (OX40L)
CD80
Alexa 647
PE
V450
MIH11
11C3.1
L307.4
AbD Serotec
Biolegend
BD Biosciences
APC Tube 5
IL15
PE
IC2471P
R&D systems
APC tube 6
CD274 (PDL1)
CD273 (PDL2)
APC
PE
29E.2A3
176611
R&D systems
All APC tubes
APC Tube 3
Supplier
Biolegend
Biolegend
Antigen
CD3
CD4
CD8
Conjugate
APC-Cy7
PE-Cy7
eFluor 605NC
Clone
HIT3a
RPA-T4
RPA-T8
PerCP-Cy5.5
APC
FITC
PE
HP-3G10
Biolegend
T-cell tube 1
CD161
IL12RB2
IL23R
CCR2
305719
218213
48607
R&D systems
R&D systems
R&D systems
PerCP-Cy5.5
APC
FITC
PB
TG2/CXCR5
T-cell tube 2
CXCR5
CCR6
CCR5
CXCR3
Biolegend
BD Biosciences
PerCP-Cy5.5
APC
FITC
PE
PB
TG2/CXCR5
Biolegend
T-cell tube 3
CXCR5
CD134 (OX40)
CD278 (ICOS)
CD279 (PD-1)
CD154 (CD40L)
443318
ISA-3
EH12.2H7
24-31
R&D systems
eBioscience
Biolegend
Biolegend
T-cell tube 4
CD25
CD127
CD31
PerCP-Cy5.5
FITC
PB
BC96
eBioRDR5
WM59
Biolegend
eBioscience
Biolegend
All T-cell tubes
11A9
45531
TG1/CXCR3
Supplier
Biolegend
Biolegend
eBioscience
R&D systems
Biolegend
Table S1B
Gene
Assay number
PBMC subset studies
UBE2D2
CASC3
UBC
HLX1
TBX21
GATA3
RORC
IFNG
IGJ
IL4
IL6
IL10
IL12A
IL12B
IL17A
IL1B
IL21
IL23
TGFB
TNFA
ICOS
IL21R
IL23R
LTA
LTB
LTBR
TNFRSF14
TNFSF14
Hs00366152_m1
Hs00904832_m1
Hs00824723_m1
Hs00172035_m1
Hs00203436_m1
Hs00231122_m1
Hs01076112_m1
Hs99999041_m1
Hs00376160_m1
Hs00174122_m1
Hs00174131_m1
Hs00961622_m1
Hs00168405_m1
Hs00233688_m1
Hs00174383_m1
Hs00174097_m1
Hs00222327_m1
Hs00372324_m1
Hs99999918_m1
Hs00174128_m1
Hs00359999_m1
Hs00986138_m1
Hs00332759_m1
Hs00236874_m1
Hs00242739_m1
Hs01101194_m1
Hs00998604_m1
Hs00542477_m1
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CSF cells and PBMC studies
Whole blood studies
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