Curriculum vitae Name: Lilah Glazer Date of Birth: 17.9.79 Place of Birth: Israel Address (at work): Department of Life Sciences, Ben-Gurion University of the Negev, P.O.B. 653. Beer-Sheva, Israel 84105 Telephone No. (at work): 972-8-6472668 Address (at home): Harimon 3, Rehovot Telephone No. (mobile): 972-54-9981245 Education 2008-present Ph.D. in Life Science. Department of Life Sciences, Ben-Gurion University of the Negev. Advisor: Prof. Amir Sagi Subject: Gastrolith matrix formation in the red claw crayfish Cherax quadricarinatus- elucidation of protein contents and functions. 2007-2008 M.Sc. in Life Sciences. Department of Life Sciences, Ben-Gurion University of the Negev. Average M.Sc. grade: 95, with distinction. 2003-2007 B.Sc. in Life Sciences. Department of Life Sciences, Ben-Gurion University of the Negev. Average B.Sc. grade: 88, with distinction. 1 Teaching experience Teaching assistant at the Department of Life Sciences, Ben Gurion University: 2010-present 2008-present 2007-present 2007-2008 Laboratory in physiology of reproduction for 3rd year biology B.Sc. 3rd year B.Sc. research project – personal instruction Biochemistry B for 2nd year biology B.Sc. Vertebrate zoology for 1st year biology B.Sc. Instructor at The Open University of Israel: 2011-present The cell: structure and function, advanced course for B.Sc. Qualifications Molecular biology and genetic engineering: DNA and RNA purification, PCR, RTPCR, DNA cloning, DNA transformation to bacteria, dsRNA preparation, real-time PCR, northern blot. Histology and immunology: Tissue fixation, parafin embedding and cutting, Hematoxylin and Eosin staining, in situ hybridization (RNA probes), cell and tissue immunostaining techniques, including immuno-fluorescence staining and immuno-gold staining for EM. Proteomics and biochemistry: Protein expression in bacteria, column separation, gel electrophoresis, western blot, peptide mass spectrometry. In-vivo: Crustacean aquaculture experimentation, dsRNA silencing by injections. Very good computer skills: Comprehensive knowledge of all office programs and bioinformatic software tools (protein 3D modelling, nucleotide and amino acid sequence analysis, MSA). General: Sex differentiation, molt and growth in decapod crustaceans, SEM operation and sample preparation. Awards Second prize of oral award in the 9th International Marine Biotechnology Conference, Qingdao, China, Oct 8-12, 2010. Deans Excelence Prise for graduate studies. Faculty of Natural Sciences, Ben-Gurion University of the Negev, 2009. The Crustacean Society best student oral presentation competition award, held during the annual meeting of the Society for Integrative and Comparative Biology, Boston, Massachusetts, USA, January 3-7, 2009. 2 Publications Glazer L., Weil S., Tom M., Roth Z., Khalaila I., Mittelman B. and Sagi A. Hemocyanin with phenoloxidase activity in the gastrolith matrix of the crayfish Cherax quadricarinatus. In prep. Pamuru, R. R., Rosen, O., Manor, R., Chung, J. S., Zmora, N., Glazer, L., Aflalo, D. E., Weil, S., Tamone, L. S. and Sagi, A. (2012). Stimulation of molt by RNA interference of the molt-inhibiting hormone in the crayfish Cherax quadricarinatus. Gen. Comp. Endocrinol. 178(2):227-236. Glazer L., Sagi A. 2012. On the involvement of proteins in the assembly of crayfish gastroliths extracellular matrix. Inv. Reprod. Dev. 56(1):57-65. Akiva-Tal A., Kababya S., Balazs Y.S., Glazer L., Berman A., Sagi A., and Schmidt A. 2011. Bioavailable calcium stabilization: the in situ molecular NMR picture of biomineralized amorphous CaCO3. Proc. Nat. Acad. Sci. USA (PNAS). 108(36):14763-14768. Glazer L., Shechter, A., Tom, M., Yudkovski, Y., Aflalo, A.D., Pamuru, R.R., Khalaila, I., Bentov, S., Berman, A., and Sagi, A. 2010. A protein involved in the assembly of an extracellular calcium storage matrix. J. Biol. Chem. 285(17): 12831-12839. Bentov, S., Weil1, S., Glazer, L., Sagi, A and Berman, A. 2010. Stabilization of amorphous calcium carbonate by phosphate rich organic matrix and by single phosphoamino acids. J. Struct. Biol. 171(2):207-15. Yudkovski, Y., Glazer, L.*, Shechter, A., Reinhardt, R., Chalifa-Caspi, V., Sagi, A., Tom, M. 2010. Multi-transcript expression patterns in the gastrolith disk and the hypodermis of the crayfish Cherax quadricarinatus at premolt. Comp. Biochem. Physiol. Part D Genomics and Proteomics. 5:171-177. *Equal contribution. Pamuru RR, Manor R, Chung JS, Zmora N, Glazer L, Aflalo ED, Weil S, Rosen O and Sagi A. Stimulation of molt by RNA interference of the molt inhibiting hormone in the crayfish Cherax quadricarinatus. In prep. Ventura T., Manor R., Aflalo E.D., Weil S., Raviv S., Glazer L., Sagi A. 2009. Temporal silencing of an androgenic-gland-specific insulin-like gene affecting phenotypic gender differences and spermatogenesis. Endocrinology. 150(3): 1278-1286. Shechter A., Glazer, L., Cheled, S., Mor, E., Weil, S., Berman A., Bentov, S., Aflalo, E.D., Khalaila, I., and Sagi A. 2008. A gastrolith protein serving a dual role in the formation of extracellular matrix containing an amorphous mineral. Proc. Nat. Acad. Sci. USA (PNAS). 105(20):7129-7134. Manor, R., Weil, S., Oren, S., Glazer, L., Aflalo, E.D., Ventura, T., Chalifa-Caspi, V., Lapidot, M., and Sagi A. 2007. Insulin and gender: an insulin-like gene expressed exclusively in the androgenic gland of the male crayfish. Gen. Comp. Endocrinol. 150(2):326-336. 3 Abstract of Presentations in Scientific Meeting Glazer L, Weil S, Mittelman B, Roth Z, Khalaila I, Tom M and Sagi A. 2012. Novel moltrelated hemocyanin family proteins from extracellular matrix of crayfish gastroliths. Crustacean Society Summer Meeting and 10th Colloquium Crustacea Decapoda Mediterranea, Athens, Greece. Tynyakov Samra, J., Ben-Tov, S., Glazer, L., Weil, S., Berman, A. and Sagi, A. 2012. Proteins of the mandible molar tooth in the red claw crayfish Cherax quadricarinatus. Crustacean Society Summer Meeting and 10th Colloquium Crustacea Decapoda Mediterranea, Athens, Greece. Glazer L, Weil S, Mittelman B, Roth Z, Khalaila I, Tom M and Sagi A. 2012. Novel moltrelated hemocyanin family proteins from extracellular matrix of crustacean gastroliths. The Society for Integrative and Comparative Biology, Charelston, South Carolina, U.S.A. Glazer L, Berman A and Sagi A. 2010. Molt-related calcium deposition in crustaceans – relevant proteins. 9th International Marine Biotechnology Conference, Qingdao, China. Glazer L, Gafni O, Weil S, Berman A and Sagi A. 2010. Chitin associated extra-cellular proteins in the gastrolith of molting crayfish. International Society of Invertebrate Reproduction and Development, Prague, Czech republic. Gafni O, Glazer L, Weil S, Berman A and Sagi A. 2010. Proteins of the bioavailable temporary calcium storage organ in the crayfish Cherax quadricarinatus. The 11th Dan Popper Symposium, Eilat, Israel. Akiva A, Glazer L, Berman A, Sagi A, Kababya S, and Schmidt A. 2010. Understanding nanostructure stabilization of ACC: A solid state NMR study of intact gastroliths from the blue lobster. The Israel Chemical Society, Tel-Aviv, Israel. Glazer L., Shechter A., Bentov S., Weil S., Sagi A., Berman A. 2009. Novel molt-related proteins with possible roles in crayfish calcium storage deposits. The Israel Chemical Society, Tel-Aviv, Israel. Glazer L., Shechter A., Berman A., Weil S., Aflalo E.D., Yudkovski Y., Tom M., Sagi A. 2009. A novel molt-related protein with a possible role in the formation of crayfish calcium storage deposits. The Society for Integrative and Comparative Biology, Boston, Massachusetts, U.S.A. Glazer L., Shechter A., Bentov S., Berman A., Weil S., Pamuru R.R., Yudkovski Y., Tom M., Sagi A., 2008. The gastrolith formation - a quest for molt related genes, their encoded proteins and their functionalities. 9 Colloquium Crustacea Decapoda Mediterranea, Torino, Italy. Shechter A., Mor E., Raviv S., Glazer L., Ramachandra Reddy P., and Sagi, A., 2007. Silencing of an ecdysone-responsive gene during crayfish molt cycle. 11th International Congress on Invertebrate Reproduction and Development, Panama City, Panama. Manor R., Weil S., Oren S., Glazer L., Aflalo E.D., Ventura T., Chalifa-Caspi V., Lapidot M. and Sagi A. 2007. Identification and characterization of an insulin-like gene expressed exclusively in the androgenic gland of the male crayfish. 8th International Marine Biotechnology Conference (IMBC), Eilat, Israel 4