Estrogenic effects of effluents on wild fish assemblages
SUPPLEMENTAL DATA
Supplemental Data, Figure S1. Map showing the locations of 15 WWTPs and streams in eastern
New York that were investigated during 2011. [Site characteristics are provided in Table 1].
Supplemental Data, Table S1. Sequences of primers used in the quantitative reverse
transcription-polymerase chain reaction (qRT-PCR) analysis.
Supplemental Data, Table S2. Estimates of community richness, diversity, density, and biomass
from quantitative fish-community surveys conducted at stream sites upstream and downstream of
effluent discharges from 10 wastewater treatment plants in eastern New York, 2011. [Site
locations are provided in Table 1 and Supplemental Data, Figure S1.]
Supplemental Data, Figure S2. The number of species populations with no differences,
significantly higher, and significantly lower estimates of population density (A) and biomass (B)
at downstream reaches (as compared to upstream reaches) from 10 WWTPs surveyed during
2011. [Site locations are identified in Table 1 and shown in Supplemental Data, Figure S1.]
Supplemental Data, Figure S3. Non-dimensional MDS ordination plot of fish assemblages using
square-root-transformed count data from upstream and downstream study reaches at 10 WWTPs
inventoried during 2011. The bubbles denote membership within like groups based on groupaveraged cluster analysis and significant (p < 0.05) Bray–Curtis similarities (ranging from 39 to
82%). [Site locations are identified in Table 1 and shown in Supplemental Data, Figure S1.]
1
bbaldigo@usgs.gov
Estrogenic effects of effluents on wild fish assemblages
Supplemental Data, Figure S1.
2
bbaldigo@usgs.gov
Estrogenic effects of effluents on wild fish assemblages
Gene
Vtg
18S
Forward primer 5’→3’
Reverse primer 5’→3’
GCTGCTGCTCCATTTCAAAA
G
CGGTTCTATTTTGTGGGTTT
CT
GTGAGAGTGCACCTCA
ACGC
CTTTTGCTCTCGTCCGT
CTT
Supplemental Data, Table S1.
3
bbaldigo@usgs.gov
Amplicon size
(bp)
160
212
Estrogenic effects of effluents on wild fish assemblages
Site code
Effluent
orientation
ny1
ny1
ny2
ny2
ny3
ny3
ny4
ny4
ny9
ny9
ny10
ny10
ny24
ny24
ny26
ny26
ny27
ny27
ny28
ny28
downstream
upstream
downstream
upstream
downstream
upstream
downstream
upstream
downstream
upstream
downstream
upstream
downstream
upstream
downstream
upstream
downstream
upstream
downstream
upstream
Richness
(No of
species)
10
10
6
4
17
19
6
7
4
1
9
8
6
4
12
13
12
12
9
8
Index of
Diversity
(1-D)
0.82
0.84
0.52
0.62
0.86
0.88
0.41
0.31
0.58
0.00
0.72
0.65
0.53
0.25
0.80
0.87
0.74
0.72
0.78
0.81
Supplemental Data, Table S2.
4
bbaldigo@usgs.gov
Density
(No./0.1ha)
Biomass
(g/0.1ha)
2,232
1,886
762
253
2,536
3,581
3,018
2,801
139
36
646
1,877
1,116
2,117
3,002
2,604
1,275
979
881
445
10,776
7,083
2,832
713
10,051
29,869
19,977
14,282
516
114
5,340
34,696
9,589
10,728
22,177
20,856
3,181
3,528
7,646
2,347
Estrogenic effects of effluents on wild fish assemblages
Supplemental Data, Figure S2.
5
bbaldigo@usgs.gov
Estrogenic effects of effluents on wild fish assemblages
Supplemental Data, Figure S3.
6
bbaldigo@usgs.gov