Terius Grandison Allison Singleton Pam Panzino Monique McGough Lab Report #1 on Water Testing at North Fork, Shenandoah River Purpose- The purpose of our experiment was to test the quality of the water at the North Fork of the Shenandoah River. Procedure- First, we picked a location on the right side of the bridge with a good riffle area. Next, Allison and I ventured into the water and placed the net in the river as Monique scrubbed the rocks in front of the net. After 30 seconds of scrubbing rocks, we came out of the water and observed the macroinvertebrates. As we collected different species we placed them into individual spots in an ice cube tray. After identifying al the organisms captured, we return the macroinvertebrates to the water and cleaned the net. Then, we tested the temperature of the water by sticking the thermometer in the water for about a minute and thirty seconds. After recording the temperature in Celsius, we tested the pH. To test the pH, we filled a test tube with water to the five millimeter line, put 10 drops of Wide Range Indicator into the test tube, mixed it all together, and compared the color of the test tube to the colors on the Octet Comparator. Next, we tested the nitrate levels in the river. To test the nitrate levels, we filled a test tube with five millimeters of water, put a nitrate #1 tablet into the test tube and let it dissolve, then put a nitrate #2 tablet in the test tube, waited five minutes, and the compared the color of the test tube to the colors on the Octet Comparator. Last but not least, we tested the dissolved oxygen. To test the dissolved oxygen in the river, we completely filled a test tube with water, put two dissolved oxygen tablets into the vial, shook it up, waited five minutes, and then compared the color of the vial to the colors on the Octet Comparator. Terius Grandison Allison Singleton Pam Panzino Monique McGough Data/Results- x-axis: Name of Organisms/ y-axis: Number of Organisms Found Terius Grandison Allison Singleton Pam Panzino Monique McGough Data/Results Continued- All listed numbers are the average of the data - Left Side Temperature: 23.3 pH: 8 Dissolved Oxygen: 4 Nitrate: 1.8 - Right Side Temperature: 24.1 pH: 7.9 Dissolved Oxygen: 1.9 Nitrate: 2 Discussion- According to the data above the quality of the water was in very good condition. Finding macroinvertebrates such as water pennies is such a high quality means just proves how great the water condition is. The nitrate was a little higher probably than usual because it had just recently rain, which would increase nitrate levels. The dissolved oxygen is probably off because we have to take into account (along with all the other tests) that human error can occur. For example, putting too much water in a test tube or put a hint of extra drops when testing pH. Things can happen. Also, using different kits can sometimes give you different results. Ways we could improve our experiment is doing a few test for things like pH to get a more accurate recording. Honestly, our group Terius Grandison Allison Singleton Pam Panzino Monique McGough did not really have any limitations while doing our experiment, but others ran into the problem of not having all the materials they needed for a specific test, resulting in the borrowing of others’ materials. Conclusions- In conclusion, the water at the North Fork of the Shenandoah River is in good condition. The pH was good, the temperature was nice, the dissolved oxygen was good, especially since we found a lot of macroinvertebrates, and the nitrate was not too bad either. Having good levels of pH, temperature, dissolved oxygen, and nitrate means life in the river can survive and all things in the river are healthy. If not, then some organisms may survive, but for the most part life will not survive. Reflections- My partners and I worked well together. We all did our share of work. While one person was doing something the other was either helping out that person or doing something else that would benefit the overall project.