Abstracts 22nd of October 2014
Nordic Biolabs AB
Which gloves will protect you in different applications?
How do you avoid skin problems related to using gloves?
This lecture is for those who want to know which glove to choose for different applications. We will
also talk about different skin problems that can occur when using gloves, why they occur and how to
avoid them.
In addition to this you will learn about requirements and how to interpret markings and symbols on
the boxes of gloves.
VWR International
"VWR Safety seminary - Examine and evaluate chemical risks in the working environment”
Scope: The purpose of examining the risks is to determine what actions are needed for the work to
be done safely. Begin with a general investigation and risk assessment. Determine how the business
should be divided into areas that can be assesses individually. Do the following three steps:
Step1: Find out what risks that exist, their dangerous properties and the frequency.
Step 2. Identify dangerous situations
Step 3. Decide what actions are needed
The safety seminar will cover all 3 steps and also include an orientation about safety data sheets;
structure, important areas etc.
Roche Diagnostics
"Translational Research and Personalized Health Care”
Personalized Health Care, PHC, is a central part of Roches global research and development strategy, and
a key component in our daily activities both in our Pharma and our Diagnostics divisions. In this lecture, we
would like to present how our two pillars of Pharma and Diagnostics are working together across the
entire value chain, from bench to bedside, to enable personalized healthcare for patients today and in
the future."
Swedish Labtech
Klara Norra Kyrkogata 31
Box 22307
104 22 Stockholm
Tel. 08-508 938 00
Fax 08-508 938 01
Webb:
E-post:
www.swedishlabtech.se
swedishlabtech@branschkansliet.se
TATAA Biocenter
High throughput single cell and subcellular expression profiling
Introduction
Biological samples are composed of large number of cells of different types. When studying
traditional samples containing many cells only the collective response of all the cells present is
measured. However, the cells may respond differently and a small subpopulation may be critical.
Today, these systems can be studied using single cell expression profiling. Here we apply single cell
profiling to study the response of astrocytes to brain trauma using mouse model. We also study
asymmetric cell division during early development of Xenopus laevis by single cell and intracellular
profiling using qPCR tomography.
Material and Methods
Single astrocytes from mice were enriched and collected by FACS using GFP under the control of the
GFAP promoter as marker. Cells were lysed (Cellulyser, TATAA Biocenter), reverse transcribed
(GrandScript, TATAA Biocenter), pre-amplified (GrandMaster PreAmp, TATAA Biocenter), and
profiled using high throughput microfluidic qPCR (BioMark, Fluidigm). Data were pre-processed and
cells were classified using multivariate methods (PCA, SOM, clustering) and correlation analysis with
the GenEx software (ver. 6, MultiD Analysis).
Results and Discussion
Astrocytes were collected from mouse brains at different time points after the induction of focal
ischemia. Each cell was profiled for the expression of 47 genes. Classification revealed astrocyte
reactivation with the formation of distinct subtypes (Figure). Single cell and subcellular blastomere
profiling revealed asymmetric cell division is induced by asymmetric distribution of key cell fate
determinants already in the fertilized cell.
Conclusions
Single cell profiling is most powerful to study complex biological samples, revealing heterogeneity
and to identify key expression pathways active in critical cell types. Power and robust flows for
experimental and analytical analysis are available, as well as highly optimized reagents.
Mikael Kubista, CEO, TATAA Biocenter
Qiagen
Next Generation Sequencing (NGS)
Next Generation Sequencing (NGS) applications are rapidly developing for routine clinical use. Until
recently whole genome sequencing and/or exome sequencing were rarely used when certain
(undiagnosed) genetic diseases were suspected. The sequencing and analysis were performed by
highly specialized research laboratories or core facilities with access expensive equipment and skilled
bioinformaticians. During the last 1-2 years smaller and cheaper NGS instrumentation have been
developed as well as more simplified preparative and analytical workflows. This development allows
for moving NGS technologies and applications into clinical laboratories, which will have a
transformational impact on healthcare and diagnostics.
Swedish Labtech
Klara Norra Kyrkogata 31
Box 22307
104 22 Stockholm
Tel. 08-508 938 00
Fax 08-508 938 01
Webb:
E-post:
www.swedishlabtech.se
swedishlabtech@branschkansliet.se
However, QIAGEN sees that current offerings for NGS workflows do not meet the demands of the
clinical environment. The company has therefore during the last year developed and launched
numerous NGS products and acquired several companies with accompanying products with the aim
to become market leader of products for clinical NGS applications. The general theme of our product
development is “Sample to Insight” where we provide streamlined workflow solutions from sample
extraction, over target enrichment, library production, to biological and clinical interpretation of the
sequencing data. We can now provide such processes for the sequencing platforms presently on the
market and with the coming GeneReader™ system QIAGEN can fully integrate and automate the
complete NGS workflow from Sample to Insight.
In the talk we will describe how the QIAGEN solutions will allow for the movement of NGS practical
workflows as well as analyses and data interpretation from the dedicated core facilities to routine
clinical laboratories.
BergmanLabora
How fit is your fat?
There has been continuous increase of interest in the constituents of food and beverages for the last
decade. Both from the regulatory bodies as well as from the consumers. What is good for us? What is
not? What is dangerous and should be avoided? And what should be banned outright?
We present three analytical scenarios to investigate the total content of fat in meat which is used as
raw material for production for example sausages and hamburgers. This is done by saponification of
fat, methylation of fatty acids to FAMEs with consequent analysis by GC/FID. The method allows at
the same time to “fingerprint” separate fatty acids and to discriminate between “good and bad” (cisand trans-) fatty acids.
Another, quick method that can determine total fat in the raw material directly in the production hall
– NIR or Near Infrared Spectroscopy – is presented here.
We are moving towards total ban of the use of steroids for treatment of animals. A method of
control of meat/fat product, which can detect very small amounts of steroids, is UHPLC with MSMS
detection is discussed.
Ilya Zelikman, PhD
__________________________________________________________________________________
Sigma-Aldrich Sweden AB
Crispr abstract
"The discovery of the type II prokaryotic CRISPR/Cas immune system has allowed for the
development of an RNA guided genome editing tool that is simple, easy and quick to implement.
Sigma Aldrich have developed a single vector system with a Cas9-GFP and a U6-guideRNA expression
cassette for CRISPR/Cas9 delivery and expression.CRISPR/Cas systems along with our CompoZR ZFN
technology provide a quick and affordable solution to targeted genome editing in a wide variety of
species and cell types. In this presentation we will discuss how both technologies can be utilised to
generate precisely targeted genome edits.”
Swedish Labtech
Klara Norra Kyrkogata 31
Box 22307
104 22 Stockholm
Tel. 08-508 938 00
Fax 08-508 938 01
Webb:
E-post:
www.swedishlabtech.se
swedishlabtech@branschkansliet.se