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Laboratory Safety
Plan
SECTION I
Purpose
This plan is intended to expand upon the KUMC’s Chemical Hygiene and Biosafety Plans (both plans are
located at here). This plan is designed to represent the specific hazards involved within this particular
laboratory and may involve multiple projects. Additionally, this plan may also serve as and/or
supplement this lab’s Biosafety Manual.
This plan shall also include specific policies and procedures established by the Principal Investigator for
all laboratory personnel. It is also intended to enhance the University’s overall Emergency Plan by
providing specific information to lab personnel on what should be done in various emergencies.
Emergency information can also be found by visiting the Emergency Management website.
This plan will be reviewed annually at minimum or whenever changes are made to laboratory
procedures. Additionally, this plan will be updated whenever physical changes to the laboratory or
regulations require updates. As you fill out the form, please delete sections which are not relevant.
Lastly, all personnel conducting research within this area on the projects specific to the PI mentioned
below will be involved in the review and trained on this plan annually and when changes occur.
BIOSAFETY LEVEL
This lab operates at the Biosafety Level marked below (place an “X” in the appropriate box):
Biosafety Level 1
Involves well-characterized agents not known to consistently cause disease in immunocompetent adult
humans, and present minimal potential hazard to laboratory personnel and the environment.
Biosafety Level 2
Involves agents that pose moderate hazards to personnel and the environment.
Biosafety Level 3
Involves indigenous or exotic agents that may cause serious or potentially lethal disease through the
inhalation route of exposure.
Biosafety Level 4
Involves dangerous and exotic agents that pose a high individual risk of aerosol-transmitted laboratory
infections and life-threatening disease that is frequently fatal, for which there are no vaccines or
treatments, or a related agent with unknown risk of transmission.
University of Kansas Medical Center (KUMC) Environment, Health & Safety (EHS)
3901 Rainbow Blvd., MS 3032 - Kansas City, KS 66160 - (913) 588-5206 - ehs@kumc.edu - website
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Laboratory Safety
Plan
Revision
Date
Revision
Version
Revisions Performed
University of Kansas Medical Center (KUMC) Environment, Health & Safety (EHS)
3901 Rainbow Blvd., MS 3032 - Kansas City, KS 66160 - (913) 588-5206 - ehs@kumc.edu - website
Page |3
Laboratory Safety
Plan
SECTION II
General Information
Date
Principal Investigator
Phone
Email
Department
Office Address
Lab Building Location
Lab Room Location
Secondary Contact
Phone
Email
List of all individuals conducting research on your project:
Last, First
Email
Phone
University of Kansas Medical Center (KUMC) Environment, Health & Safety (EHS)
3901 Rainbow Blvd., MS 3032 - Kansas City, KS 66160 - (913) 588-5206 - ehs@kumc.edu - website
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Laboratory Safety
Plan
ADDITIONAL CONTACT INFORMATION:
EHS Office
913-588-1081
EHS Email
ehs@kumc.edu
EHS Website
here
EHS Chalk Training
here
Radiation Concerns
913-588-6126 or RSO@kumc.edu
Biohazard Concerns
913-588-8110 or 913-588-5206
Chemical Concerns
913-588-5194
Facilities Management
913-588-7928
Facilities Work Request
here
Emergency Management
here
Housekeeping
913-588-3535
In case of emergency call KUMC Police at 913-588-5030 or 911 from a campus phone.
Please note: if 911 is dialed from a cell phone, KCK Fire and Police will be notified.
The below represents actions our lab will take in the event of a hazardous spill:
Biological Spill
The spill will be decontaminated with appropriate disinfection (e.g. 10% bleach
solution) for a minimum of 30 minutes. The decontamination process will be
repeated once. The EHS office (913-588-1081) will be notified of the spill.
Chemical Spill
Prevent the material from spreading if possible and evacuate the area. Notify
the Police and/or EHS staff as soon as possible (Police: 911 on campus phone or
913-588-5030. EHS: 913-588-1081). Be available either by phone or in person
to answer questions that the EHS staff may have
Radiological Spill
EHS will be contacted immediately if the spill occurs in areas outside of the
laboratory or if the spill is large. For a smaller spill inside the laboratory, lab
personnel may call EHS or may clean up the spill on their own. Clean up would
involve containing the spill with absorbent materials, wiping down the area
with an appropriate cleaner (like Rad. Decon Spray) and then surveying the area
to verify that the cleaning was effective.
University of Kansas Medical Center (KUMC) Environment, Health & Safety (EHS)
3901 Rainbow Blvd., MS 3032 - Kansas City, KS 66160 - (913) 588-5206 - ehs@kumc.edu - website
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Laboratory Safety
Plan
SECTION III
Hazard Declaration
Below are all hazards in which this laboratory is associated.
Select Pathogenic Agents
Select Toxins
Other Biological Toxins
Etiological Agents
Bloodborne Pathogens
Recombinant / Synthetic DNA
Hazardous Chemicals
Hazardous / Chemotherapeutic Drugs
DEA Regulated Drugs
Investigational Drugs
Human Cells / Tissue
Non-Human Primate Cells / Tissues
Non-Human Primate Research
Animal Research
Transgenic / Knockout Animal Models
Radioactive Isotopes
Radiation Producing Devices
Human Research (Clinical Trails)
Aerosol Hazards
Sharps Hazards
Flammable Materials
Human Gene Therapy
Additional hazards not listed above:
University of Kansas Medical Center (KUMC) Environment, Health & Safety (EHS)
3901 Rainbow Blvd., MS 3032 - Kansas City, KS 66160 - (913) 588-5206 - ehs@kumc.edu - website
Page |6
Laboratory Safety
Plan
SECTION IV
Relevant Definitions
Biohazard Materials
Infectious or potentially infectious biological material that is capable of causing human disease.
Biosafety
Laboratory practices in place to help mitigate laboratory-acquired infections.
Bloodborne Pathogens
Pathogenic microorganisms that are present in human blood and can cause disease in humans.
Hazardous Waste
A waste chemical defined by the EPA which poses substantial or potential threats to public
health or the environment. These waste chemicals can be found on certain EPA lists or exhibit
the characteristics of ignitability, reactivity, corrosivity or toxicity. These lists can be found
here.
Occupational Exposure
Reasonably anticipated skin, eye, mucous membrane, or parenteral contact with blood,
potentially infectious materials or chemicals/drugs that may result from the performance of an
employee’s duties.
Occupational Health Clinic
Campus health clinic that provides medical services during work hours (8am-5pm, M-F).
Principal Investigator
KUMC faculty member responsible for research underway in the laboratory.
Recombinant DNA Molecules
Molecules that are constructed outside living cells by joining natural or synthetic DNA segments
to DNA molecules that can replicate in a living cell; or molecules that result from the replication
of those described above.
Universal Precautions
Treat all biological materials as if they are infected with some sort of biohazard capable of
causing human disease, regardless of known status.
University of Kansas Medical Center (KUMC) Environment, Health & Safety (EHS)
3901 Rainbow Blvd., MS 3032 - Kansas City, KS 66160 - (913) 588-5206 - ehs@kumc.edu - website
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Laboratory Safety
Plan
SECTION V
Safety Equipment
FIRE EXTINGUISHERS
Fire extinguishers of the appropriate size and type have been installed throughout KUMC and
are to be checked monthly by Facilities Management. After a fire extinguisher is used, contact
the EHS Office so that a replacement fire extinguisher is put in its place. Below is a description
of the location of the nearest fire extinguisher:
Although your main goal is to evacuate the area and not fight the fire, should you need to
utilize a fire extinguisher, the acronym “PASS” can help you remember:
P = Pull the pin breaking the plastic seal;
A = Aim at the base of the fire;
S = Squeeze the handles together; and
S = Sweep from side to side
FIRST AID KITS
These kits are to be utilized when injuries occur that can be immediately treated. Below is a
description of the location of the nearest first aid kit:
SAFETY DATA SHEETS – SDS (formally Material Safety Data Sheets – MSDS)
SDS’s are maintained for all chemical and drug hazards, and made readily available at all times
for laboratory personnel. Below is a description of the location of all SDS’s for this laboratory:
University of Kansas Medical Center (KUMC) Environment, Health & Safety (EHS)
3901 Rainbow Blvd., MS 3032 - Kansas City, KS 66160 - (913) 588-5206 - ehs@kumc.edu - website
Page |8
Laboratory Safety
Plan
AUTOMATED EXTERNAL DEFIBRILLATOR (AED)
There is a portable electronic device that automatically diagnoses potentially
life- threatening cardiac arrhythmias of ventricular fibrillation and ventricular
tachycardia in a patient, and is able to treat the patient through defibrillation,
the application of electrical therapy which stops the arrhythmia, allowing the
heart to reestablish an effective rhythm. Below is a description of the location
of the nearest AED:
LIFE SLIDERS
These are devices that can be used to transport an injured, disabled or
unconscious patient short distances. Below is a description of the location
of the nearest Life Slider:
EYE WASH STATIONS & SAFETY SHOWERS
These shall be utilized when individuals have been externally exposed to hazardous materials.
They are intended to help flush the hazard from the body and possibly eliminate transfer of the
hazard to the remainder of the body. It is important to know whether the hazards present in
the laboratory cannot or should not be mixed with water before an accident occurs. Each eye
station’s lines shall be cleared weekly by lab personnel. Below is a description of the location of
the nearest eye wash & safety shower stations:
GAS SHUT-OFF VALVES & REGULATORS
In the event that gas lines need to be shut off, the valve or regulator shall be utilized. This
prevents the build-up of gas within the area. Below is a description as to the location of the gas
shut-off valves:
University of Kansas Medical Center (KUMC) Environment, Health & Safety (EHS)
3901 Rainbow Blvd., MS 3032 - Kansas City, KS 66160 - (913) 588-5206 - ehs@kumc.edu - website
Page |9
Laboratory Safety
Plan
CHEMICAL FUME HOODS
Chemical fume hoods are to be utilized for use with volatile chemicals, gases and drugs or any
materials in which an airborne hazard may be present or introduced by the handling method
(heating, for example). All fume hoods shall be certified for performance annually. For
additional information on fume hoods, please visit here.
BIOLOGICAL SAFETY CABINETS (BSC)
Biological Safety Cabinets (BSC’s) are to be utilized for use with biological hazards and NOT
volatile chemicals, gases and drugs. They provide protection for the personnel, environment
and/or product. All BSC’s shall to be certified for performance annually. For additional
information on BSC’s, please see the KUMC Biosafety Plan found here and refer to Appendix A
of the Biosafety in Microbiological and Biomedical Laboratories (BMBL) found here.
PERSONAL PROTECTIVE EQUIPMENT (PPE)
PPE must be donned when working with or in an area that contains hazards. The type of PPE
utilized will correlate to the type of hazard involved. Below is the required PPE that will be
utilized and its location:
SPILL CLEAN-UP MATERIALS/KIT
The spill kit shall be used upon when a spill has occurred and lab feels safe doing so.
Additionally, the EHS office shall be notified that a spill has occurred and the details
surrounding the incident. If you feel you are unable to perform the spill clean-up, evacuate the
lab (informing others to evacuate as well) and notify the EHS Office. Below is a description as to
the location of the nearest spill kit:
University of Kansas Medical Center (KUMC) Environment, Health & Safety (EHS)
3901 Rainbow Blvd., MS 3032 - Kansas City, KS 66160 - (913) 588-5206 - ehs@kumc.edu - website
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Laboratory Safety
Plan
SECTION VI
Evacuation & Shelters
EVACUATION
Fire emergency response is defined by the acronym “RACE”. If you discover a fire, see smoke
or flames; follow the RACE procedures in this order:
Remove/Rescue all persons in immediate danger to safety. Do not use elevators. Use
stairs.
Activate manual pull station AND call or have someone call 911 (KUMC landline) or 913-5885030 (cell phone).
Close doors, windows or other openings to prevent the spread of smoke and fire.
Extinguish the fire (if you are trained and able) / Evacuate the building if necessary and
report to designated meeting area.
In the event of a fire that cannot be contained, evacuation should be conducted. Visitors and
personnel should move completely out of the affected building and to either an adjoining
building beyond the fire doors or outside the facility to your department’s designated
Emergency Assembly Point (EAP) The EHS Office has assigned outdoor EAPs that departments
may utilize; to see this listing, click here. Remain in Assembly Area until ALL CLEAR has been
given.
In Case of Fire: The EAP for this lab is located (enter location below): Laboratory personnel will
perform the following before beginning research work:
TAKING SHELTER
In the event of severe weather, stay away from windows! Proceed to the basement
or ground floor interior hallways of the building. Shelter areas are designated with
the green Severe Weather Shelter sign.
University of Kansas Medical Center (KUMC) Environment, Health & Safety (EHS)
3901 Rainbow Blvd., MS 3032 - Kansas City, KS 66160 - (913) 588-5206 - ehs@kumc.edu - website
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Laboratory Safety
Plan
SECTION VII
Medical Surveillance
Medical Surveillance is defined as a method to detect and eliminate the underlying causes such
as hazards or exposures of any discovered trends. This method can be shall be implemented
before work has begun, ongoing and in the event of occupational exposure.
PREVENTATIVE
Laboratory personnel will undergo the following before beginning research work:
ONGOING
Laboratory personnel will undergo the following as ongoing medical surveillance:
POST EXPOSURE
Laboratory personnel will perform the following post exposure response:
University of Kansas Medical Center (KUMC) Environment, Health & Safety (EHS)
3901 Rainbow Blvd., MS 3032 - Kansas City, KS 66160 - (913) 588-5206 - ehs@kumc.edu - website
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Laboratory Safety
Plan
SECTION VIII
Laboratory Waste
GENERAL
A quick reference guide for managing hazardous waste and other concerns is located here.
BROKEN GLASS
Information regarding the disposal of broken glass can be found here.
CHEMICAL & DRUG
Chemical or Drug waste will be labeled appropriately and discarded by submission of a
completed chemical hazard pick-up form found here. Additionally, an online chemical database
can be a helpful resource and is found here. The location of the chemical waste in the lab is:
BIOLOGICAL
Biological waste that hasn’t been autoclaved or rendered non-infectious by… will be contained
in a red bag or bin designated for biological waste and discarded by submission of a completed
biohazard hazard pick-up form found here. The location of the biological waste in the lab is:
RADIOACTIVE
Radiological waste will be labeled, segregated and handled as required by the Radiation Safety
Manual. It will be discarded by contacting the Radiation Safety Officer at ehs@kumc.edu. The
location of the radioactive waste in the lab is:
University of Kansas Medical Center (KUMC) Environment, Health & Safety (EHS)
3901 Rainbow Blvd., MS 3032 - Kansas City, KS 66160 - (913) 588-5206 - ehs@kumc.edu - website
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Laboratory Safety
Plan
SECTION IX
Personnel Training
All laboratory personnel will be required to successfully complete the annual below online
trainings:
General Safety Training
Biosafety Safety Training
Bloodborne Pathogen Training
Personal Protective Equipment
Hazard Communication & RCRA Chemical Safety
Laboratory personnel will also complete the additional training listed below to ensure they fully
understand the hazards involved and procedures for mitigating occupational exposure:
University of Kansas Medical Center (KUMC) Environment, Health & Safety (EHS)
3901 Rainbow Blvd., MS 3032 - Kansas City, KS 66160 - (913) 588-5206 - ehs@kumc.edu - website
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Laboratory Safety
Plan
SECTION X
Additional Comments
Please provide any additional comments in which you feel necessary for personnel to be informed in
regards to safety precautions (cage changing procedures, animal handling, special training, vaccines,
precautions for pregnant women etc.). This information will greatly assist in mitigating an
occupational exposure/injury/death. Feel free to be as specific, detailed and descriptive as possible
as this risk assessments integrity relies on the information you provide (this is clearly stated within
the BMBL and NIH Guidelines ).
University of Kansas Medical Center (KUMC) Environment, Health & Safety (EHS)
3901 Rainbow Blvd., MS 3032 - Kansas City, KS 66160 - (913) 588-5206 - ehs@kumc.edu - website
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Laboratory Safety
Plan
Principal Investigator’s Certification
I hereby certify that I have reviewed the contents of this plan and that it reflects my current operating
policy for the laboratories described in this document. I also confirm the biosafety level of
containment is appropriate for all identified biohazard risks that are found within the laboratories,
based on the BMBL and NIH Guidelines.
Principal Investigator (Print)
Principal Investigator (Signature)
Date
Appendices
Example: Project Approval Forms
Example: IACUC ACUP
Example: Human Subjects Approval Form
Example: Specific SOP’s
Example: Radiation Permit
Example: Drawing showing evacuation routes
University of Kansas Medical Center (KUMC) Environment, Health & Safety (EHS)
3901 Rainbow Blvd., MS 3032 - Kansas City, KS 66160 - (913) 588-5206 - ehs@kumc.edu - website