PREPARATION OF THIN FILMS FOR ELEMENTAL
ANALYSIS OF NAIL POLISH BY WAVELENGTH
DISPERSIVE X-RAY SPECTROSCOPY
Supplementary Material
Andrea C. McWilliams,1* Al A. Martin,2 Mike A. Levine1, Keith E. Levine1, Lauren Felder,1
Daniel J. Young,1 James M. Harrington1
1
Trace Inorganics Department, RTI International, 3040 E Cornwallis Rd., PO Box 12194,
Research Triangle Park, NC 27709
2
Thermo Scientific, West Palm Beach, FL
* Corresponding author: acm@rti.org, phone: 1-919-485-5520
Table S1. Nail polish samples analyzed in this study.
Laboratory
Replicates
Country
Color
ID
Shimmer
1
10
France
Silver
2
10
Trinidad &
Dark Red
Tobago
3
10
USA
Pink
4
5
Bulgaria
Dark Blue
5
5
China
Blue
6
5
Poland
Yellow
7
3
Canada
Blue
8
3
Turkey
Neon Pink
9
3
Taiwan
Bronze
10
3
Australia
Dark Blue
11
3
Brazil
Green
Gloss
12
10
Canada
Neon Pink
13
10
Australia
Light Blue
14
10
Luxembourg
Neon Pink
15
5
France
Purple
16
5
UK
Light Pink
17
5
USA
Light Blue
18
3
Taiwan
Dark Blue
19
3
Poland
Light Pink
20
3
South Korea
Neon Pink
21
3
China
Black
Table S2. Nail polish samples selected for ICP-OES comparability analysis.
Laboratory ID
Country
Color
Shimmer
2
Trinidad & Tobago
Dark Red
4
Bulgaria
Dark Blue
9
Taiwan
Bronze
22
Sweden
Pink
23
Finlanda
Orange
24
Germany
Pink
25
USA
Blue
26
USA
Yellow
Gloss
a
20
South Korea
Neon Pink
27
Turkey
Pink
28
USA
Yellow
29
Luxembourga
Green
30
Taiwan
Red
31
Australia
Pink
32
China
Purple
Sample was prepared and analyzed in duplicate.
Table S3. WDXRF instrumental parameters for analysis of nail polish samples
Elements
kV
mA
Crystal
Detectora
Collimator
Filter
Mask
(mm)
a
F, Na, Mg
30
80
AX03
FPC
0.40
None
29
Al, Si
30
50
AX03
FPC
0.15
None
29
P, S, Cl
40
60
Ge111
FPC
0.15
None
29
K, Ca, Ti
40
40
LiF200
FPC
0.15
None
29
V, Mn, Fe
40
60
LiF220
FPC
0.15
None
29
FPC: flow proportional counter.
Table S4. Microwave digestion program for nail polish samples.
Power (W)
Ramp Time
Hold Time
Temp (°C) Pressure (PSI)
(min)
(min)
400
10
10
100
250
800
10
10
160
400
1600
10
10
180
600
Table S5. Instrumental parameters for analysis of nail polish thin films by ICP-OES
Instrument setting
Value
RF Power
1150 W
Nebulizer flow
0.70 L/min
Auxiliary flow
0.50 L/min
Viewing mode
Axial
Sample introduction system
HF resistant
Analysis pump rate
50 rpm
Figure S1. Effect of inverting thin films on elemental analysis of Na, Ti, and Fe. Results shown
for nail polish sample 14 show inversion for replicates 2, 3, and 8.
Content (% W/W)
0.0500
Na
0.0400
0.0300
0.0200
0.0100
0.0000
1
2
3
4
5 6 7
Replicate
8
9
10 11
Content (% w/w)
3
Ti
2.5
2
1.5
1
0.5
0
Content (% w/w)
1
2
3
0.1600
0.1400
0.1200
0.1000
0.0800
0.0600
0.0400
0.0200
0.0000
4
5
6
7
Replicate
8
9
10
11
Fe
1
2
3
4
5 6 7
Replicate
8
9
10 11