Respiratory Complications Following Surgical Correction of Brachycephalic Airway Syndrome Senior Seminar Paper Cornell University College of Veterinary Medicine Blake Hefter 2/12/14 Dr. James Flanders Dr. Theresa Southard Keywords: Aspiration Pneumonia, Brachycephalic Airway Syndrome, ALI/ARDS ABSTRACT A 10 month old castrated male English bulldog presented to the Cornell University Hospital for Animals Soft Tissue Surgery Service for surgical correction of a previously diagnosed urethral prolapse. At that time, the owners were also interested in an evaluation for Brachycephalic Airway Syndrome. Once anesthetized, the airway of the patient was evaluated. An elongated soft palate along with everted laryngeal saccules were visualized. The palate was shortened approximately 1.5 cm and the saccules were resected. The patient recovered from anesthesia, and was discharged the next day. He was brought back to the Cornell University Hospital for Animals Emergency Service the following day for respiratory distress and fever. The owner said he had vomited a few times since discharge. He went into cardiac arrest while in the emergency room and had open chest CPR performed. He was successfully resuscitated. He was placed on a mechanical ventilator in critical care until the owners chose humane euthanasia. We believe aspiration pneumonia to be the cause of the clinical signs and pathologic changes observed. HISTORY A 10-month-old castrated male English bulldog presented to the Cornell University Hospital for Animals Soft Tissue Surgery Service on referral for surgical correction of a urethral prolapse. According to the owner, for about 1 month prior presentation they noticed what was described as a purple flower like structure at the tip of the patient’s penis. After examination by the local veterinarian, it was diagnosed as a urethral prolapse. The local veterinarian attempted manual reduction, but when it recurred two weeks later, the owner was referred to the Cornell Universtiy Hospotal for Animals Soft Tissue Surgery Service. When the client arrived, they wanted the patient to be evaluated for Brachycephalic Airway Syndrome in addition to the urethral prolapse. The clients owned another bulldog that had a soft palate resection performed through the Soft Tissue Surgery Service, so they were aware of the components of Brachycephalic Airway Syndrome. PHYSICAL EXAM The physical exam proved there to be a prolapsed urethra. It was manually reduced, but returned immediately. Additionally, sterterous breathing was noted. The patient was also noted to be highly overconditioned with a body condition score of 9/9. The remainder of the exam was unremarkable. Due to the patient’s signalment, Brachycephalic Airway Syndrome was the top differential for sterterous breathing. If this were a non-brachycephalic dog, differentials such as foreign body, nasal or laryngeal mass (abscess, granuloma, neoplasia), laryngeal paralysis, or upper airway infection would be considered. TREATMENT In order to definitely diagnose Brachycephalic Airway Syndrome, evaluation of the larynx must take place under general anesthesia. The patient was fasted during the overnight stay in the hospital, and the evaluation took place the following day. While under anesthesia, the patient was observed to have an elongated soft palate, along with everted laryngeal saccules. These are two of the six components of Brachycephalic Airway Syndrome. At the time of evaluation, surgery was performed to correct these developmental abnormalities. Additionally, the urethral proplapse was corrected. The patient recovered from anesthesia slightly hypoxic, so he was placed into an oxygen cage. When his blood oxygen saturation was within the normal reference range, approximately three hours after surgery, he was taken out of the oxygen cage. At that time, the patient was bright, alert, and responsive. His vital parameters (temperature, pulse rate, respiratory rate, mucous membrane color, etc.) were within the normal reference range. He was fed one can of wet food in small bites. He did well overnight and into the next morning until 8am when he was observed to vomit and become nauseous. He was given a dose of metoclopramide, and no further nausea was seen. He was observed to eat more wet food without difficulty and was discharged to his owner later that morning. OUTCOME The owners called the CUHA Emergency Service in the evening on the day of discharge. They were worried because the patient had vomited twice since being home, was febrile, and was in respiratory distress. The owners were instructed to bring the patient in for examination, but since they lived a long distance away they could not come back until the next morning. The owners dropped the patient off at 6:30 am the next morning to the care of the Emergency Service. While being stabilized, the dog went into cardiac arrest. Upon admission, the owner chose a green code for resuscitation. This means upon arrest measures up to and including open chest cardiac massage should be attempted. While the patient was in cardiac arrest and the emergency room doctor was putting on sterile gloves, closed chest compressions were performed. As soon as the doctors were ready and the site was prepared, an incision through the chest wall and into the thoracic cavity was made. The ribs were separated, and cardiac massage was performed. Following return of spontaneous circulation, the patient was brought into the surgery suite for sterile lavage and closure of the thoracotomy. At that time, a tracheal tube and arterial catheter were placed. Postoperative radiographs were performed which revealed diffuse consolidation in most lung lobes. The patient was attached to the mechanical ventilator and admitted into the critical care ward. The patient continued to decline over the next two days, and ultimately the owners chose humane euthanasia. A full necropsy was performed, which revealed diffuse consolidation and atelectasis of most lung lobes. Differential diagnoses for these problems are pneumonia, cardiogenic and non-cardiogenic pulmonary edema, torsion, neoplasia, airway obstruction, and trauma. Samples of lung were taken for histopathology in an attempt to determine the underlying cause of the clinical signs. The histopathology revealed ninety percent of the airspace and interstitum was filled with fibrin, degenerate neutrophils, and phagocytized bacterial cocci. Fibrinosuppurative pneumonia consistant with aspiration was the final diagnosis. DISCUSSION Aspiration pneumonia is acute or chronic inflammation of the lungs and airways due to inhalation of irritant material. The most commonly noted irritant materials are food particles and gastric acid. The pathologic changes seen occur in three stages. The first stage occurs immediately after aspiration. During this phase, damage to the airways and pulmonary parenchyma is a result of the aspirate. This direct tissue damage triggers the activation of cytokines, most notably TNF-a, IL-1, and IL-6, and other inflammatory mediators. The inflammation then leads to necrosis of type I alveolar cells, pulmonary hemorrhage, and increased vascular permeability resulting in pulmonary edema. The second stage begins 4 to 6 hours after aspiration and lasts for 12 to 48 hours. It is characterized by infiltration of neutrophils into the alveoli and pulmonary interstitium. The continued leakage of proteins furthers the development of pulmonary edema. Neutrophil activation, and release of further proinflammatory cytokines add to the vascular leakage. The third and final phase involves bacterial colonization of the airways and pulmonary parenchyma (1) Clinical signs from aspiration vary widely from patient to patient. Some patients will have no signs, others can develop a mild pneumonia, and some end in systemic inflammatory response and ultimately death. In retrospective study of aspiration pneumonia in 88 dogs, regardless of etiology, 77% of the cases survived until discharge. The remaining 23% either died in the hospital, or were euthanized (2) Depending on what is aspirated, stomach acid, food particles, or both, the pathologic changes and severity differ. In a study involving intratracheal administration of stomach acid, food particles, or both, arterial oxygenation was dramatically reduced following administration of the both acid and food with PaO2/FiO2 ratios meeting the criteria for clinical Acute Respiratory Distress Syndrome (ARDS) in the 24 hr period following aspiration. Additionally the inflammatory response following the administration of both food and acid was more profound than administration of either food or acid. (3) Aspiration pneumonia is known to be a causal factor of acute lung injury (ALI) or acute respiratory distress syndrome. ALI is a syndrome of pulmonary inflammation and edema resulting in acute respiratory failure. ARDS is the severe manifestation of ALI, with the major difference between the two being the degree of hypoxemia as defined by the ratio of arterial oxygen tension (PaO2) to fractional inspired oxygen concentration (FiO2). ALI and ARDS are similar to aspiration pneumonia since there are many underlying etiologies, however the pathologic findings in all ALI and ARDS cases are consistent. Causes of ARDS are aspiration, pneumonia, toxic inhalation, asphyxiation, sepsis, shock, severe trauma, or drug related toxicity. The development of ALI/ARDS is secondary to an exaggerated inflammatory response. The phases of ALI/ARDS are described based on morphologic changes seen in 3 stages, known as the exudative, proliferative, and fibrotic stage. The exudative stage of begins with pulmonary vascular leakage and inflammatory cell infiltration. The architecture of the lung changes as type I alveolar pneumocytes are damaged. These pneumocytes are primarily responsible for gas exchange. As they can no longer perform their function, type II pneumocytes make an attempt to repair the damaged area in order to allow for gas exchange. Type II pneumocytes primarily produce surfactant, however during this phase of ARDS they abandon this responsibility. These changes in pneumocyte function are the cause of hyaline membrane formation histologically, as well as alveolar collapse. The first phase of ALI/ARDS begins at 24-48 hours post injury, and lasts about 1 week in humans. Organization of the inflammatory exudate and development of fibrosis characterize the second phase. Type II pneumocytes continue to increase in numbers in an attempt to repair the damaged epithelial surfaces. Additionally, fibroblasts proliferate and lead to narrowing and collapse of the airway. Histologically, changes in the lung becomes more evident. The pulmonary parenchyma becomes dilated and edematous, the hyaline membrane formation progresses, and ultimately the airspace fills with fibrin and cellular debris. The fibrotic phase is the final stage of ALI/ARDS. This phase involves collagen deposition in the alveoli, vessels, and interstitium. Little is known about this phase in veterinary patients due to the current mortality occuring during the first phase of ARDS being close to 100%. Additionally, ALI and ARDS are associated with the manifestation of MODS, or multi organ dysfunction syndrome. No matter the etiology of ALI or ARDS, they are characterized by a local and exaggerated inflammatory response. These inflammatory mediators then spill over into the circulation, and if exaggerated enough, SIRS, or systemic inflammatory response syndrome occurs. The result of this syndrome is the change in vascular endothelium, blood flow, tissue perfusion, and cellular oxygenation. Inflammatory mediators such as histamine, interlukens, TNF, and cytokines are not only the cause of these changes, but also the chemicals that perpetuate the cycle that ultimately leads to organ dysfunction. (4, 5, 6, 7, 8, 9) Neither ALI nor ARDS were diagnosed by histopathology in the patient of this report. Characteristic microscopic changes were not present, however ALI and ARDS cannot be ruled out. The patient was in distress for only three days before euthanasia was performed. These microscopic changes may have been starting to occur, but were arrested when the patient was euthanized. The patient’s clinical signs and diagnostic tests placed this dog in ARDS. Additionally, there were no histologic signs of MODS, however the patients bloodwork while in Critical Care suggested multiple organ systems were also beginning to fail. Selected References: 1-Marik, P. E. (2001). Aspiration Pneumonitis and Aspiration Pneumonia. The New England Journal Medicine, (344), 665-671. 2-Kogan, D. A., Johnson, L. R., Jandrey, K. E., & Pollard, R. E. (2008). Clinical, clinicopathologic, and radiographic findings in dogs with aspiration pneumonia: 88 cases (2004-2006). J Am Vet Med Assoc, 233(11), 1742-7. 3- Raghavendran, K., Nemzek, J., Napolitano, L. M., Knight, P. R. (2011). Aspiration Induced Lung Injury. Critical Care Medicine, 39(4), 818–826. 4- Declue, A. E., Cohn, L. A. (2007). Acute Respiratory Distress Syndrome in Dogs and Cats: A Review of Clinical Findings and Pathophysiology. Journal of Veterinary Emergency and Critical Care, (17.4) 340-47. 5-Tamashefski, J. (1990) Pulmonary Pathology of the Adult Respiratory Distress Syndrome. Clinics in Chest Medicine, (11), 593–619. 6- Bellingan, G. J. (2002) The Pulmonary Physician in Critical Care * 6: The Pathogenesis of ALI/ARDS. Thorax, (57.6) 540-46. 7-Anderson, W., Thielen, K. (1992) Correlative Study of Adult Respiratory Distress Syndrome by Light, Scanning, and Transmission Electron Microscopy. Ultrastruct Pathology, (16) 615–628. 8-Lopez, A,. Lane, I,. Hanna, P. (1995) Adult Respiratory Distress Syndrome in a Dog With Necrotizing Pancreatitis. Canadian Veterinary Journal, (36), 240–241. 9-Hunter, T. (2001) Acute Respiratory Distress Syndrome in a 10-year-old Dog. 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