Dr. Kerry Bloom & Dr. Blaire Steinwand Fall 2014 TR, 12:30-01:45 GS200 Section 007 Lecture Schedule: Part I (Bloom) Date # Pre-lecture assigned readings Objectives 1 IntroductionGenes, Genomes and Genetic Analysis Chap. 1 Explain how genetics effects our lives Tu 8/19 Understand the terms: Personalized medicine, Genetically modified organisms, stem cells Kerry Bloom 2 Th DNA: the Molecule of Heredity Chap 7 223-231 Define Gene, Chromosome, allele Define genotype, phenotype Explain why DNA is the heredity material Draw the chemical structure of DNA and define the specific features responsible for base pairing, strand complementarity, antiparallel helix How traits are transmitted: Mendelian Inheritance Chap 2 25-44 Define the features of Mendels experiments Explain the Law of Segregation Explain the Law of Independent Assortment Explain what a di-hybrid cross is and what it tells us. 8/21 3 Tu 8/26 Recommended readings Assignments Understand Dominance and Recessive 4 Mitosis Chap 3 63-81 Mitosis Th 8/28 5 Tu The chromosome theory of Inheritance Chap 3 81-96 9/02 6 Understand the Unit of Inheritance Explain how sex chromosomes help us understand heredity processes Explain the process of chromosome nondisjunction Gene Interaction Chap 4 105-142 Th Define incomplete penetrance and variable expressivity Define epistatis and pleiotropy 9/04 For lectures 7,8 work linkage mapping 7 Genetic Chap 5 143Linkage and 164 Tu Chromosome Mapping 9/09 Define gene linkage and map distance Explain how recombination frequency is determined 8 . Tetrads Chap 5 170177 Th 9/11 Monday Review Session Coker 201 6:30 to 8:00 Tu 9/16 EXAM 1 (Lectures 1-8) 9 Th Molecular Mechanisms of Mutation and DNA Repair Chap. 12 383408 Define mutation and why they are heritable. Explain the difference between induced and spontaneous mutations. Define DNA repair Molecular Biology of DNA Recombination and Exchange Chap 12 410421 Define the Holliday model for DNA recombination. Chromosome Structure Chap 11 359382 Explain the difference between DNA and the chromosome. Define Chromatin. Define Epigenetics Chromosome Aberrations Chap 13 422443 How does non-disjunction lead to changes in chromosome number. Explain the mechanisms that lead to chromosome rearrangement 9/18 10 Tu 9/23 11 Explain what is meant by Gene Conversion Th 9/25 12 Tu 09/30 13 Th Chromosome Dynamics and Fractals 10/02 Recitation: 10/2-3 Exam review questions Tu 10/07 EXAM 2 (Lectures 8-13) What is meant by DNA fluctuation. Define 3C and how this was used to map higher order chromosome conformation 14 Th How Genes TravelBacterial Genetics -Describe the different mechanisms of horizontal gene transfer Blaire Steinwand - Build hypotheses, design an experiment using an appropriate assay, and predict the results pp. 184-191 - Explain how one can examine the phenotypes of bacteria 10/09 - Describe bacterial conjugation No Recitations 15 Bacterial Genetics II + Tu introduction to Gene 10/14 Expression pp. 261-264 (section 8.1) - Compare and contrast F-, F+, and Hfr bacterial strains - Explain how the interrupted mating technique allows us to determine gene order - Describe the central dogma - Describe the differences between DNA and RNA - Predict the results of an experiment based on a given hypothesis Th 10/16 16 Tu - pp. 191-194 - “mRNA Brenner experiment explained.pdf” (External Links/Sakai) Gene ExpressionTranscription pp. 264-265 - Explain how the RNA polymerase knows where to start transcription - Homework due Wed, 10/23 - Compare and contrast prokaryotic and eukaryotic transcription mechanisms pp. 270-271; 273- - MG- Promoter quiz, due 274; Tue, 10/22, 10:00am Fig. 8.6 - Homework due Wed, 10/30 -. Describe the event staking place during transcription Th MG (MasteringGenetics) RNA quiz, due Tue, 10/15 10:00am Fall Break 10/21 17 - Gene ExpressionTranslation 10/23 - Explain how pre-mRNA is processed - Describe the mechanisms of RNA splicing and define alternative splicing - Define and identify an open reading frame - Explain the forces that shape a protein and determine its function - Define the genetic code Recitation: 10/23-24 Bacterial Genetics activity pp. 278-284 18 Tu 10/28 19 Th Gene ExpressionThe Nature of the Genetic Code Gene Regulation in Prokaryotes Figures 9.7; 9.9; and 9.10 (and the relevant text) - 199 (including Figure 6.12) - Describe the events that take place during translation - Define the properties of the genetic code pp. 312-322 (sections 9.4 and 9.5) - MG- Translation quiz, due Tue, 10/29, 10:00am Homework due Wed, 11/06 - Explain how the code has been deciphered - Predict the effect of different mutations on protein function - List the various ways that gene expression can be regulated -PeerWise questions (Gene expression), due Tue, 11/5 pp. 463-471; 473 - Describe what the lac operon does and why E. coli regulates its expression -PeerWise answers and feedback (Gene expression), due Sun, 11/10 10/30 -Describe how lactose induces the expression of the lac operon - Deduce the genes affected in the lac operon by their phenotypes and genetic behavior Recitation: 10/30-31 Gene Expression activity 20 Tu Gene Regulation in Prokaryotes II - Homework due Wed, 11/13 11/04 21 Th Gene Regulation in Eukaryotes - Describe cis- and trans- active elements involved in eukaryotic regulation pp. 501-504; 509512 - Define epigenetics and explain how epigenetic mechanisms regulate transcription 11/06 - Explain imprinting and chromatin remodeling Recitation: 11/6-7 Regulation of Gene Expression in prokaryotes Tu EXAM 3 (Lectures 13-20) 11/11 22 Gene Regulation in - Discuss the effect of the environment on the epigenome pp. 283; 285-286; 512-516 -PeerWise questions (Gene expression), due Tue, 11/26 Th - Explain the role of alternative splicing in gene regulation, and how it is regulated Eukaryotes II 11/13 -PeerWise answers and feedback (Gene expression), due Tue, 12/03 - Describe the role of miRNA in gene regulation - - Homework due Wed, 11/20 Recitation: 11/13-14 Regulation of Gene Expression in Eukaryotes 23 Tu Recombinant DNA Technology pp. 531-534 11/18 24 Th 11/20 - Distinguish between forward and reverse genetics - Define developmental genetics and explain the importance of model organisms Recombinant DNA Technology: A PHD in 75 minutes - Design a protocol for gene cloning - Distinguish between genomic and complimentary DNA - Describe the main properties of cancer cells - Define the three groups of cancer promoting mutations 11/25 Thanksgiving Break - Define the three groups of cancer promoting mutations Tu 12/02 Molecular Genetics of Cancer II Tu 12/10 EXAM 4 & CUMULATIVE FINAL EXAM 12:00 pm - Describe the genomic approaches in cancer therapy - MG- Restriction quiz, due Tue, 11/19, 10:00am - - Explain how to construct molecules to address biological questions Recitation: 11/20-21 Exam review questions 25 Molecular Genetics of Tu Cancer Th 11/27 26 - pp. 535-539 - Homework due Wed, 11/27