Curriculum vitae
ZAFFAGNINI MIRKO
Gender: Male
Born: 15 February 1976
Nationality: Italian
Married, 1 child
mirko.zaffagnini3@unibo.it
Education and Diplomas
2002: Bachelor in Biological Science, University of Bologna, Italy
2006: PhD in Functional Biology of Molecular and Cellular Systems, University of Bologna.
Research experience
7/2009 – current: Postdoctoral fellowship, Laboratory of Molecular Plant Physiology directed by Prof.
Paolo Trost and Paolo Pupillo, University of Bologna, Italy
7/2006 - 6/2009: Postdoctoral fellowship, Laboratory of Redox Signaling directed by Dr. Stephane D.
Lemaire, University Paris-Sud 11, France
1/2006 - 6/2006: Postdoctoral fellowship, University of Bologna, Italy, Laboratory of Molecular Plant
Physiology directed by Prof. Paolo Trost e Paolo Pupillo
10/2004 - 5/2005: Ph. D Stage, Laboratory of Redox Signaling directed by Dr. Myroslawa MiginiacMaslow, University Paris-Sud 11, France
1/2003 - 12/2005: Ph. D. fellow funded by Italian Ministry of Education and Research, directed by Prof.
Paolo Trost e Paolo Pupillo, Title: “Redox regulation of photosynthetic glyceraldehyde-3-phosphate
dehydrogenase”, University of Bologna, Italy
Editorial work
Ad hoc reviewer: Molecular Plant, Plant Science, BMC Plant Biology, Biologia Plantarum,
Publications in peer-reviewed journals
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Sparla F, Fermani S, Falini G, Zaffagnini M, Ripamonti A, Sabatino P, Pupillo P, Trost P (2004)
Coenzyme site-directed mutants of photosynthetic A4-GAPDH show selectively reduced NADPHdependent catalysis, similar to regulatory AB-GAPDH inhibited by oxidized thioredoxin. J Mol Biol
340: 1025-1037
Sparla* F, Zaffagnini* M, Wedel N, Scheibe R, Pupillo P, Trost P. (2005) Regulation of photosynthetic
GAPDH dissected by mutants. Plant Physiol 138: 2210-2219. *equal contribution
Michelet L, Zaffagnini M, Marchand C, Collin V, Decottignies P, Tsan P, Lancelin JM, Trost P,
Miginiac-Maslow M, Noctor G, Lemaire SD (2005). Glutathionylation of chloroplast thioredoxin f is a
redox signaling mechanism in plants. Proc Natl Acad Sci U S A 102: 16478-16483
Michelet L, Zaffagnini M, Massot V, Keryer E, Vanacker H, M Miginiac-Maslow M, IssakidisBourget E, Lemaire SD (2006) Thioredoxins, glutaredoxins, and glutathionylation: new crosstalks to
explore. Photosynth Res 89: 225-245
Trost P, Fermani S, Marri L, Zaffagnini M, Falini G, Scagliarini S, Pupillo P, Sparla F (2006)
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Thioredoxin-dependent regulation of photosynthetic glyceraldehyde-3-phosphate dehydrogenase:
autonomous vs. CP12-dependent mechanisms. Photosynth Res 89: 263-275
Zaffagnini M, Michelet L, Marchand C, Sparla F, Decottignies P, Le Marechal P, Miginiac-Maslow M,
Noctor G, Trost P, Lemaire SD (2007) The thioredoxin-independent isoform of chloroplastic
glyceraldehyde-3-phosphate dehydrogenase is selectively regulated by glutathionylation. FEBS J 274:
212-226
Lemaire SD, Michelet L, Zaffagnini M, Massot V, Issakidis-Bourget E (2007) Thioredoxins in
chloroplasts. Curr Genetics 51: 343-365
Zaffagnini M, Michelet L, Massot V, Trost P, Lemaire SD (2008) Biochemical characterization of
glutaredoxins from Chlamydomonas reinhardtii reveals the unique properties of a chloroplastic CGFStype glutaredoxin. J Biol Chem 283: 8868-8876
Michelet L, Zaffagnini M, Vanacker H, Le Marèchal P, Marchand C, Schroda M, Lemaire SD,
Decottignies P (2008) In vivo targets of S-thiolation in Chlamydomonas reinhardtii. J Biol Chem 283:
21571-21578
Marri* L, Zaffagnini* M, Collin* V, Issakidis-Bourget E, Lemaire SD, Pupillo P, Sparla F, MiginiacMaslow M, Trost P (2009) Prompt and easy activation by specific thioredoxins of Calvin cycle enzymes
of Arabidopsis thaliana associated in the GAPDH/CP12/PRK supramolecular complex. Mol Plant 2:
259-269: * equal contribution.
Gao X, Bedhomme M, Veyel D, Zaffagnini M, Lemaire SD (2009) Methods for analysis of protein
glutathionylation and their application to photosynthetic organisms. Mol Plant: 218-235
Couturier* J, Koh* CS, Zaffagnini* M, Winger A, Gualberto JM, Corbier C, Decottignies P, Jacquot
JP, Lemaire SD, Didierjean C, Rouhier N (2009) Structure-function relationship of the chloroplastic
glutaredoxin S12 with an atypical WCSYS active site. J Biol Chem 284: 9299-310. *equal
contribution.
Tarrago L, Laugier E, Zaffagnini M, Marchand C, Le Maréchal P, Rouhier N, Lemaire SD, Rey P
(2009) Regeneration mechanisms of Arabidopsis thaliana methionine sulfoxide reductases B by
glutaredoxins and thioredoxins. J Biol Chem 284: 18963-18971.
Terauchi AM, Lu S-F, Zaffagnini M, Tappa S, Hirasawa M, Tripathy JN, Knaff DB, Farmer PJ,
Lemaire SD, Toshiharu Hase T, Merchant SS. (2009) Pattern of expression and substrate specificity of
chloroplast ferredoxins from Chlamydomonas Reinhardtii. J Biol Chem 284: 25867-25878
Née G, Zaffagnini M, Trost P, Issakidis-Bourguet E. (2009). Redox regulation of chloroplastic glucose6-phosphate dehydrogenase: a new role for f-type thioredoxin. FEBS Letters 583: 2827-2832
Bedhomme M, Zaffagnini M, Marchand CH, Gao XH, Moslonka-Lefebvre M, Michelet L,
Decottignies P, Lemaire SD. (2009) Regulation by glutathionylation of isocitrate lyase from
Chlamydomonas reinhardtii. J Biol Chem 284: 36282-36291
Tarrago L, Laugier E, Zaffagnini M, Marchand CH, Le Marechal P, Lemaire SD, Rey P. (2010) The
plant thioredoxin CDSP32 regenerates 1-CYS methionine sulfoxide reductase B activity through the
direct reduction of sulfenic acid. J Biol Chem 285: 14964-14972
Gao XH, Zaffagnini M, Bedhomme M, Michelet L, Cassier-Chauvat C, Decottignies P, Lemaire SD.
(2010) Biochemical characterization of glutaredoxins from Chlamydomonas reinhardtii: Kinetics and
specificity in deglutathionylation reactions. FEBS Letters 584: 2242-2248
Zaffagnini M, Bedhomme M, Couturier J, Gao X-H, Marchand CH, Rouhier N, Trost P, Lemaire SD.
(2012) Glutaredoxin S12: unique properties for redox signaling. Antioxid Redox Signal 16: 17-32
Zaffagnini M, Bedhomme M, Marchand CH, Morisse S, Trost P, Lemaire SD. (2012) Redox regulation
in photosynthetic organisms: focus on glutathionylation. Antioxid Redox Signal 16: 567-586
Zaffagnini M, Bedhomme M, Groni H, Marchand CH, Puppo C, Gontero B, Cassier-Chauvat C,
Decottignies P, Lemaire SD. Glutathionylation in the Photosynthetic Model Organism Chlamydomonas
reinhardtii: A Proteomic Survey. Mol Cell Proteomics 2012 Feb;11(2):M111. 014142
Zaffagnini M, Bedhomme M, Lemaire SD, Trost P (2012) The emerging roles of protein
glutathionylation in chloroplasts. Plant Sci 185-186: 86-96
Bedhomme M, Adamo M, Marchand CH, Couturier J, Rouhier N, Lemaire SD, Zaffagnini* M, Trost*
P. (2012) Glutathionylation of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the model
plant Arabidopsis thaliana is reversed by both glutaredoxins and thioredoxins in vitro. Biochem J 445:
337-47. *equal correspondence
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Book Chapters
1. Michelet L, Zaffagnini M, Lemaire SD (2009). Thioredoxins and Related Proteins. In: E. HARRIS; D.
STERN; The Chlamydomonas Sourcebook: Organellar and Metabolic Processes. vol. 2, p. 401-444
Witman Eds. Elsevier
2. Gao XG, Bedhomme M, Michelet L, Zaffagnini M, Lemaire SD. (2009) Glutathionylation in
photosynthetic organisms. Advances in Botanical Research, Vol. 52, Burlington: Academic Press, pp.
363-403IF: 2,319
Invited presentations
2004: SIFV-SIGA Congress, Lecce (Italy).
2006: FISV Congress, II Meeting in Bertinoro (Italy).
2006: Annual Congress of GDR (Groupment de recherche) 2006, Orsay (France).
2006: Annual Congress of ANR (Agence nationale de recherché), Nancy (France).
2007: Department of Biology, University di Bologna (Italy).
2007: FISV Congress, Riva del Garda (Italy).
2009: SIBV Congress, Verona (Italy).
2009: Department of Experimental and Evolutionary Biology, University di Bologna (Italy).
2010: SFPhi Congress, Paris (France).
2012: Accademy of Georgofili, Firenze (Italy)
2012: University di Napoli and University of Verona: invited seminars
Prizes and Awards
2007: Prize Franca Rasi Caldogno of the Italian Society of Plant Physiology for the best PhD thesis,
academic years: 2006-2007
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