1 Expanded Description of Materials and Methods 2 Site Description 3 This study focuses on assessing changes to lake ecosystems in the uplands east of the 4 Mackenzie Delta, near the northern town of Inuvik, Northwest Territories, Canada (Fig. 1). 5 Climate in the region is seasonally extreme. Winters are cold due to the shielding effect of the 6 cordillera, which blocks warm, maritime Pacific air, ensuring cold Arctic air masses dominate 7 the region (Dyke, 2000). In contrast, during the summer months, southern air masses move 8 northward, giving the region the warmest summer temperatures in Canada for its latitude (Dyke, 9 2000). The study region was covered by the Wisconsinan ice sheet which reached a maximum 10 extent ~38.8 thousand years ago (Duk-Rodkin & Lemmen, 2000). The region is underlain by 11 thick, continuous permafrost, with taliks that penetrate through the permafrost present under 12 lakes that do not freeze to the bottom due to the insulating properties of water (Burn, 2002). 13 Near-surface permafrost is enriched with solutes (particularly calcium and sulphate, which are 14 glacially derived from carbonate and shale bedrocks) in comparison to the overlying active layer 15 (Kokelj & Burn, 2003, 2005). Vegetation communities common in the lowland shrub tundra just 16 north of treeline include primarily members of the Cyperaceae, especially Eriophorum and 17 Carex (Ritchie, 1984), although shrub birch (Betula nana subsp. exilis), green alder (Alnus 18 viridis subsp. fruticosa), and willow (Salix spp.) are common (MacDonald, 2000). Patches of 19 black (Picea mariana) and white (Picea glauca) spruce in a tundra matrix are common where 20 climatically favourable sites exist (Ritchie, 1984). 21 22 Water chemistry analyses, sediment core collection and diatom analyses 23 Samples for water chemistry analyses were collected from the central portion of the lake 24 approximately 1 m below the surface. Samples for determination of major ion concentrations 25 were analyzed by ion chromatography at the Taiga Environmental Laboratory (Yellowknife, 26 NT). Samples for dissolved and unfiltered total phosphorus (TP) concentration determination 27 were analyzed using the colorimetric method for low yield results at the National Laboratory for 28 Environmental Testing, Canadian Centre for Inland Waters (Burlington, ON). For lakes from 29 which sediment was sampled through the winter ice, nutrient samples were collected the 30 following open-water season. Lakes 2a, 2b and 5b were not re-sampled, and thus unfiltered TP 31 concentrations were taken from Thompson (2009). Further water chemistry analyses for the 12 32 study lakes are available in Kokelj et al. (2009b) and Thompson (2009), including other nutrients 33 such as total dissolved nitrogen, which was not sampled at time of sediment sampling. 34 Sediment cores from the 12 lakes studied were collected from the centre (deepest 35 location) of each lake over a two year period, in April of 2007 (coring through the late winter 36 ice) and in July of 2008 (coring from an inflatable raft). In all cases lakes were accessed by 37 helicopter. Sediment cores were collected using a Glew-type (1989) gravity corer (internal 38 diameter 7.62 cm) and sectioned using a Glew (1988) vertical extruder. To obtain high temporal 39 resolution paleolimnological profiles, cores were sectioned at 0.25 cm (for the top 15 cm) and 40 0.5 cm (for the remainder of the core) intervals, and stored in individual WhirlPak® bags at 4 °C 41 until analysis. Sediment age estimation was established on 10-16 sedimentary intervals from 42 each core using 210Pb and 137Cs radioisotopes following standard methods for gamma dating 43 (Schelske et al., 1994; Appleby, 2001). For all sediment cores, the constant rate of supply (CRS) 44 model (Appleby & Oldfield, 1978) was used to calculate dates, and whenever possible, the peak 45 in 137Cs (corresponding to 1963) was used to corroborate the 210Pb dates (Appleby, 2001). For 46 each core the cumulative dry weight of the sediment core was compared to the log-transformed 47 unsupported 210Pb activity, in order to detect changes in sediment accumulation over time 48 (deviation from a linear fit used to detect changes). Linear regression for all cores were found to 49 be strong (r2 >0.60), with the slump-affected lakes showing no difference from reference sites, 50 with the exception of Lake 9b, which showed a somewhat less robust relationship (r2 = 0.45). 51 Laboratory methods for siliceous indicator identification followed Battarbee et al. (2001). 52 In summary, sediment was digested in a mixture of sulphuric and nitric acids, and heated in a 53 water bath to facilitate the digestion of the organic matrix. After being allowed to settle, samples 54 were rinsed daily until a near neutral pH was established. Aliquots of diatom slurry were plated 55 onto coverslips, and mounted on microscope slides using the high-refractive index mounting 56 medium Naphrax© (Brunel Microscopes, Wiltshire, UK). For each sedimentary interval, at least 57 300 diatom valves were identified and enumerated along transects at 1000x magnification. 58 Diatoms were identified using a variety of sources (primarily Krammer & Lange-Bertalot, 1991, 59 1997, 1999, 2000; Cumming et al., 1995; Fallu et al., 1999). 60 61 Statistical Analyses 62 Climate data from Inuvik were obtained from the Adjusted Historical Canadian Climate 63 Database (http://www.cccma.ec.gc.ca/hccd/). The SiZer (Sonderegger, 2010) package for the R 64 statistical environment (R Development Core Team, 2010) was used to conduct a piecewise 65 linear regression (Toms & Lesperance, 2003) on these climate data, with 95% confidence 66 intervals determined by bootstrapping (n=1000). Longer term climate reconstructions (~A.D. 67 1750 to 1988) based on dendrochronological analyses of samples (Szeicz & MacDonald, 1995; 68 Wilson et al., 2007) were obtained online from the National Oceanic and Atmospheric 69 Administration (NOAA) National Climate Data Center. 70 Relative frequency diagrams of the most common diatom taxa were prepared using the 71 computer program Tilia v1.7.16 (Grimm, 2011), with a constrained incremental sum of squares 72 (CONISS) cluster analysis included (Grimm, 1987). Detrended canonical correspondence 73 analyses (DCCA) were conducted using CANOCO v4.5 (ter Braak & Šmilauer, 2002). The 74 approach used here follows Smol et al. (2005) where compositional change in diatoms, 75 chrysophytes, chironomids and cladocerans over the past 150 years was quantified and compared 76 among a suite of Arctic lakes. For all sedimentary diatom profiles, DCCAs were conducted using 77 square-root transformations, no down-weighting of rare taxa and detrending by segments. 78 Trends in the relative abundance of benthic fragilarioid taxa in each lake over time were 79 standardized using z-scores (i.e. standard scores) and combined into two records (reference and 80 slump-affected sites) by calculating the mean z-score for each 210Pb year estimate where multiple 81 samples existed. These standardized trends were compared to the yearly air temperature records 82 (mean annual and mean winter) from the Inuvik climate station using Pearson correlation 83 analyses (temperature data were not smoothed prior to analyses). Significance levels were 84 adjusted using Bonferroni probabilities. Hill’s N2 diversity (Hill, 1973) determinations were 85 conducted using the vegan package for R (Oksanen et al., 2010). 86 87 References 88 Appleby P.G. (2001) Chronostratigraphic techniques in recent sediments. 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