Levendusky – Anatomy and Physiology II – Fetal Pig Dissection (Unit: Reproductive System) Fetal Pig Dissection Group Members: Group Member Roles: ____________________________________ ____________________________________ ____________________________________ ____________________________________ ____________________________________ ____________________________________ ____________________________________ ____________________________________ Team Member Roles: Reader – reads lab directions, makes sure group is correctly following the procedure Recorder – writes down all answers/records group input on the lab sheets *Answers are provided as a group effort* Team Leader – gathers any materials needed, makes sure group is on task/watches time, asks any questions that may arise Dissector – dissects the fetal pig following directions from the reader and team leader Background: Mammals are vertebrates having hair on their body and mammary glands to nourish their young. The majority are placental mammals in which the developing young, or fetus, grows inside the female's uterus while attached to a membrane called the placenta. The placenta is the source of food and oxygen for the fetus, and it also serves to get rid of fetal wastes. The dissection of the fetal pig in the laboratory is important because pigs and humans have the same level of metabolism and have similar organs and systems. Also, fetal pigs are a byproduct of the pork food industry so they aren't raised for dissection purposes, and they are relatively inexpensive. Objectives: Identify important external structures of the fetal pig. Identify major structures associated with a fetal pig's urogenital system. Compare the functions of certain organs in a fetal mammal with those of an adult mammal. Materials: preserved fetal pig, dissecting pan, dissecting kit, dissecting pins, string, plastic bag, metric ruler, paper towels Pre-lab: Before observing internal or external structures of the fetal pig, use your dissection manual, textbook, and dissection notebook to answer the pre-lab questions on the fetal pig. You may have to refer to more than one dissection manual to answer all the questions so trade and share with other dissection groups. Safety: *Review your safety contract before beginning the dissection. *Any violation of the safety contract will result in ejection from the lab and a zero for your evidence grade(s). *Wear your lab apron and safety goggles at all times. *Watch your time and be sure to clean up all equipment and working area each day before leaving. 1 Levendusky – Anatomy and Physiology II – Fetal Pig Dissection (Unit: Reproductive System) Fetal Pig Dissection Student Directions Part I - External Anatomy 1. Obtain a fetal pig and rinse off the excess preservative by holding it under running water. Lay the pig on its side in the dissecting pan and locate dorsal, ventral,& lateral surfaces. Also locate the anterior and posterior ends. 2. A fetal pig has not been born yet, but its approximate age since conception can be estimated by measuring its length. Measure your pig's length from the tip of its snout to the base of its tail and record this on your hand-in. Use the length/age chart on this sheet or the inside cover of your dissection manual to determine the age of your fetal pig & record this. 3. Examine the pig's head. Locate the eyelids and the external ears or pinnae. Find the external nostrils. 4. Study the pig's appendages and examine the pig's toes. Count and record the number of toes and the type of hoof the pig has. 5. Locate the umbilical cord. With scissors, cut across the cord about 1 cm from the body. Examine the 3 openings in the umbilical cord. The largest is the umbilical vein, which carries blood from the placenta to the fetus. The two smaller openings are the umbilical arteries which carry blood from the fetus to the placenta. 6. Lift the pig's tail to find the anus. Study the ventral surface of the pig and note the tiny bumps called mammary papillary. These are present in both sexes. In the female these structures connect to the mammary glands. 7. Determine the sex of your pig by locating the urogenital opening through which liquid wastes and reproductive cells pass. In the male, the opening is on the ventral surface of the pig just posterior to the umbilical cord. In the female, the opening is ventral to the anus. Record the sex of your pig. 8. Carefully lay the pig on one side in your dissecting pan and cut away the skin from the side of the face and upper neck to expose the masseter muscle that works the jaw, lymph nodes, and salivary glands. Label these on your hand-in. 9. With scissors, make a 3-cm incision in each corner of the pig's mouth. Your incision should extend posteriorly through the jaw. 10. Spread the jaws open and examine the tongue. 11. Observe the palate on the roof of the mouth. The anterior part of the palate is the hard palate, while the posterior part is the soft palate. 12. Locate the epiglottis, a cone-shaped structure at the back of the mouth. Above the epiglottis, find the round opening of the nasopharynx. This cavity carries air from the nostrils to the trachea, a large tube in the thoracic which supplies air to the lungs. 13. Dorsal to the glottis, find the opening to the esophagus. Examine the tongue and note tiny projections called sensory papillae. 14. Examine the teeth of the pig. Canine teeth are longer for tearing food, while incisor are shorter and used for biting. Pigs are omnivores, eating plants and animals. 15. Label the drawing of the inside of the pig's mouth. 2 Levendusky – Anatomy and Physiology II – Fetal Pig Dissection (Unit: Reproductive System) Part II - The Incision 1. Be sure to wear your lab apron and eye cover. Obtain your dissecting equipment and pig from the supply cart. 2. Place the fetal pig ventral side up in the dissecting tray. 3. Tie a string securely around a front limb. Run the string under the tray, pull it tight, and tie it to the other front limb. Repeat this procedure with the hind limbs to hold the legs apart so you can examine internal structures. 4. Study the diagram at the right. The dashed lines numbered 1-5 show the first set of incisions that you will make. To find the exact location for the incision marked 2, press along the thorax with your fingers to find the lower edge of the ribs. This is where you will make incision 2. 5. With scissors, make the incisions in order, beginning with 1. Be sure to keep the tips of your scissors pointed upward because a deep cut will destroy the organs below. Also, remember to cut away from yourself. 6. After you have made your incisions through the body wall, you will see the peritoneum, a thin layer of tissue that lines the body cavity. Cut through the peritoneum along the incision lines. 7. Spread the flaps of the body wall apart. Cut the umbilical vein which extends through the liver. 8. Once the vein is cut, carefully pull the flap of skin, including the end of the umbilical cord between the hind legs. You are now able to see the organs of the abdominal cavity. Urogenital System 1. Be sure to wear your lab apron and eye cover. 2. Remove the digestive organs to study the excretory (urinary) and reproductive organs that make up the urogenital system. 3. Locate the large, bean-shaped kidneys lying against the dorsal body wall. Notice that they are covered by the peritoneum. Kidneys filter wastes from blood. 4. Find the ureters, tubes which extend from the kidneys to the bag-like urinary bladder. The urinary bladder lies between the umbilical arteries and temporarily stores liquid wastes filtered from the blood. 5. Lift the urinary bladder to find the urethra, the tube which carries urine out of the body. Follow the urethra to the urogenital opening on the outside of the pig's body. 6. Make sure that incision #6 extends all the way to the anus but be careful to not cut too deep and damage the internal organs. 3 Levendusky – Anatomy and Physiology II – Fetal Pig Dissection (Unit: Reproductive System) 7. Follow the directions below for locating the excretory and reproductive organs in either a male or female pig. When you finish observing the organs in a pig of one sex, exchange specimens with another classmate to view the organs in a pig of the opposite sex. Male System 8. In the male pig, locate the two scrotal sacs at the posterior end of the pig. If the pig is in the later stages of development, you will find a testis in each sac. If the pig is in an early stage of development, the oval-shaped testes will be in the abdominal cavity. These testes have not yet descended into the scrotal sacs. 9. On each testis, find the coiled epididymis. Sperm cells produced in the testis pass through the epididymis and into a tube called the vas deferens. This tube crosses over a ureter and enters the urethra. 10. Follow the urethra to the penis, a muscular tube lying just below the skin posterior to the umbilical cord. In mammals, the penis is the organ that transfers sperm. 11. Label the diagram of the male urogenital system on the handout. Female System 12. In the female pig, find the two bean-shaped ovaries at the posterior end of the abdominal cavity. Observe the coiled Fallopian tube attached to each ovary, which carries eggs from the ovary. 13. Follow the Fallopian tube to the uterus. The uterus is dorsal to the urinary bladder and the urethra. 14. Trace the uterus to a muscular tube called the vagina. The vagina will appear as a continuation of the uterus. Sperm from the male are deposited into this organ during mating. The vagina and the urethra open into a common area called the urogenital sinus. This cavity opens to the outside at the urogenital opening. 15. Label the diagram of the female urogenital system on the handout. *Important: Make sure that you view both the male and female fetal pig reproductive system. You do not know if your practical will be on a male or female fetal pig.* When you have completed your study of the urogenital system of both sexes, clean up your materials and work area. [Make sure to wash and dry all dissection tools, case and dissection tray. Spray down and wipe your table.] Dispose of the dissected specimen in the appropriate garbage. Thoroughly wash your hands with soap. 4