TABLE SI
Comparison of expected and observed AMF 18S rDNA gene templates and T-RF products in terms of relative peak area ratioa) using PCR-TRFLP for
amplification of plasmid DNA clone 02 / plasmid DNA clone 01 mixtures, and plasmid DNA clone 01 / plasmid DNA clone 02 mixtures
b)
c)
Plasmid DNA Proportion
clone 02/clone 01
Plasmid DNA Proportion
clone 01/clone 02
Proportion of plasmid DNA template mixtures
1 / 0.2
1 / 0.4
1 / 0.8
1/1
1 / 0.2
1 / 0.4
1 / 0.8
1/1
Expected plasmid DNA template-to-product ratios
5
2.5
1.25
1
5
2.5
1.25
1
(Proportion of relative peak area ratios)
(0.8333/0.1667)
Observed proportion of Relative peak area ratio I
f)
a
12.79 ± 0.0002
d)
j)
d)
(0.7143/0.2867)
a
d)
(0.5556/0.4444)
a
d)
(0.50/0.50)
a
e)
(0.8333/0.1667)
b
e)
(0.7143/0.2867)
b
e)
(0.5556/0.4444)
c
(0.50/0.50)
e)
c
4.42 ± 0.0002
2.07 ± 0.0056
1.62 ± 0.0024
4.51 ± 0.0058
2.17 ± 0.0051
0.82 ± 0.0026
0.62 ± 0.0024
(0.8154/0.1846)
(0.6739/0.3261)
(0.6176/0.3824)
(0.8187/0.1813)
(0.6847/0.3152)
(0.45/0.55)
(0.3824/0.6176)
(Proportion of relative peak area ratios)
(0.9275/0.0725)
Observed proportion of Relative peak area ratio II g)
12.02 ± 0.0001
4.06 ± 0.0005
1.83 ± 0.0009
1.42 ± 0.0047
4.52 ± 0.0001
2.17 ± 0.0108
0.82 ± 0.0122
0.71 ± 0.0047
(Proportion of relative peak area ratios)
(0.9232/0.0768)
(0.8023/0.1977)
(0.6470/0.3530)
(0.5865/0.4135)
(0.8189/0.1811)
(0.6848/0.3152)
(0.45/0.55)
(0.4135/0.5865)
Observed proportion of Relative peak area ratio III
h)
b
c
b
c
b
b
b
b
b
a
b
b
c
b
b
b
11.14 ± 0.0016
4.00 ± 0.0014
1.80 ± 0.0109
1.39 ± 0.0115
5.23 ± 0.0097
2.27 ± 0.0084
0.95 ± 0.0120
0.72 ± 0.0115
(Proportion of relative peak area ratios)
(0.9176/0.0824)
(0.80/0.20)
(0.6432/0.3568)
(0.5822/0.4178)
(0.8396/0.1604)
(0.6938/0.3062)
(0.4867/0.5133)
(0.4178/0.5822)
Observed proportion of Relative peak area ratio IV i)
11.14 ± 0.0002
3.88 ± 0.0002
1.79 ± 0.0054
1.29 ± 0.0015
5.08 ± 0.0011
2.58 ± 0.0033
1.03 ± 0.0057
0.77 ± 0.0015
(Proportion of relative peak area ratios)
(0.9176/0.0824)
(0.7952/0.2048)
(0.6422/0.3578)
(0.5635/0.4365)
(0.8356/0.1644)
(0.7206/0.2794)
(0.5070/0.4930)
(0.4365/0.5635)
a)
f)
c
a
a
a
a
The data were generated from dividing relative peak area ratio of plasmid DNA clone 02 by relative peak area ratio of plasmid DNA clone 01.
Expected relative peak area ratio in pairwise mixing of plasmid DNA clone 02/plasmid DNA clone 01.
Expected relative peak area ratio in pairwise mixing of plasmid DNA clone 01/plasmid DNA clone 02.
Observed proportion of relative peak area ratio I were determined by PCR-TRFLP amplifications of AMF plasmid DNA mixtures that were mixed with Ri-T-DNA-transformed carrot root genomic DNA (non-purified DNA).
g)
Observed proportion of relative peak area ratio II were determined by PCR-TRFLP amplifications of AMF plasmid DNA mixtures that were mixed with Ri-T-DNA-transformed carrot root genomic DNA purified by ethanol precipitation.
h)
i)
b
The data were generated from dividing relative peak area ratio of plasmid DNA clone 01 by relative peak area ratio of plasmid DNA clone 02.
d)
e)
d
Relative peak area ratio was calculated from dividing individual T-RF peak area values by the sum of all peak area values in the corresponding profile.
b)
c)
c
Observed proportion of relative peak area ratio III were determined by PCR-TRFLP amplifications of AMF plasmid DNA mixtures that were mixed with Ri-T-DNA-transformed carrot root genomic DNA purified by phenol chloroform.
Observed proportion of relative peak area ratio IV were determined by PCR-TRFLP amplifications of AMF plasmid DNA mixtures that were mixed with TE buffer.
j)
Means ± standard deviations were calculated from data in triplicates runs for each of triplicate PCR reactions, and mean values within a column that are statistically significant (P<0.05) are indicated by a different letter.