1
Supporting information
2
3
Tissue-Specific Metabolic Responses of Cyprinus flammans to Copper
4
Ming-Yan Hu  Yang-Fang Ye  Liang-Yi Xue  Ze-Yuan Tang
5
1
6
Fig. S1. PCA scores plots derived from 1H NMR data for gill, liver, and muscle extracts from the control
7
group (triangles) and Cu-treated group (circles)
8
9
10
Fig. S2. OPLS-DA scores plots derived from 1H NMR data for gill, liver, and muscle extracts from the
control groups (stars) and 0.25 mg/L Cu-treated groups (circles)
11
12
Table S1 Correlation coefficients from OPLS-DA of metabolic profiles of gill, liver, and muscle
13
extracts of C. flammans after 3.5 and 6.0 mg/L Cu exposure
14
2
Fig. S1
R2X=0.64, Q2=0.53
0.25 mg/L
control
R2X=0.63, Q2=0.46
0.89 mg/L
40
t[2]
t[2]
20
Gill
0
R2X=0.60, Q2=0.37
1.53 mg/L
control
60 control
-20
-40
40
40
20
20
t[2]
15
0
0
-20
-20
-40
-40
-60
-60 -40 -20
0
20 40
-60 -40 -20
60
0
SIMCA-P+ 11 - 2014/12/4 12:22:32
-60 -40 -20
-20
40
R2X=0.69, Q2=0.56
1.53 mg/L
control
20
0
0
-20
-20
-40
-40
-40
-20
0
20
-60 -40 -20 0 20 40 60
-80 -60 -40 -20 0 20 40 60 80
40
t[1]
t[1]
t[1]
SIMCA-P+ 11 - 2014-5-26 下 午 13:39:39
SIMCA-P+ 11 - 2014-5-26 下 午 13:37:57
R2X=0.73, Q2=0.62
0.25 mg/L
control
40
R2X=0.83, Q2=0.73
0.89 mg/L
40 control
20
20
0
-20
-40
-40
-80 -60 -40 -20 0 20 40 60 80
16
0
-20
R2X=0.85, Q2=0.76
1.53 mg/L
control
20
t[2]
t[2]
t[2]
SIMCA-P+ 11 - 2014/12/4 12:23:50
Muscle
20 40 60
SIMCA-P+ 11 - 2014-5-26 下 午 13:34:52
20
0
0
t[1]
R2X=0.64, Q2=0.45
0.89 mg/L
control
t[2]
t[2]
60
SIMCA-P+ 11 - 2014-5-26 下 午 13:32:47
R2X=0.42, Q2=0.12
0.25 mg/L
control
20
Liver
40
t[2]
40
20
t[1]
t[1]
0
-20
-50
t[1]
0
50
-100
-50
0
50
100
t[1]
t[1]
SIMCA-P+ 11 - 2014/12/4 12:20:31
SIMCA-P+ 11 - 2014-5-26 下 午 13:43:23
17
18
3
SIMCA-P+ 11 - 2014-5-26 下 午 13:44:58
Fig. S2
R2X=0.55, Q2=0.51, p=0.07
40
40
Gill
t[2]O
t[2]O
40
Liver
0
-20
0
-40
-40
-20
0
20
40
Muscle
0
-20
-20
-40
R2X=0.66, Q2=0.32, p=0.28
20
20
20
20
R2X=0.34, Q2=0.54, p=0.06
t[2]O
19
-40
-40 -30 -20 -10 0 10 20 30 40
t[1]P
t[1]P
SIMCA-P+ 11 - 2014/11/27 10:00:30
4
0
20
40
60
t[1]P
SIMCA-P+ 11 - 2014/11/27 9:36:57
21
-60 -40 -20
SIMCA-P+ 11 - 2014/11/27 16:32:12
22
Table S1 Correlation coefficients from OPLS-DA of metabolic profiles of gill, liver, and muscle
23
extracts of C. flammans after Cu exposure.
Correlation coefficients (r a)
Metabolites
Gill
treated group VS control
Liver
Muscle
0.89 mg/L
1.53 mg/L
0.89 mg/L
1.53 mg/L
0.89 mg/L
1.53 mg/L
Isoleucine
―b
―
-0.85
-0.74
―
―
Leucine
―
―
-0.95
-0.78
―
―
Valine
―
―
-0.95
-0.87
―
―
Lactate
―
―
0.77
―
0.81
0.87
Alanine
―
-0.70
-0.95
-0.97
―
0.93
Acetate
0.99
0.99
―
―
0.90
0.91
Methionine
-0.76
-0.91
―
―
#c
#
Citrate
-0.84
―
#
#
-0.87
-0.94
Lysine
―
―
-0.88
-0.81
#
#
Choline-O-sulfate
―
0.78
―
―
#
#
Choline
―
0.76
―
―
―
―
Taurine
-0.91
―
0.79
0.94
―
0.91
Creatine
―
―
―
―
―
0.74
Glycine
―
―
―
―
―
0.77
Glucose
―
0.94
0.77
0.89
―
―
Tyrosine
―
―
-0.94
-0.78
―
―
Histidine
-0.69
-0.69
-0.87
-0.83
―
―
5
Phenylalanine
―
―
-0.90
-0.69
#
#
Adenosine
―
0.90
―
―
-0.86
-0.68
Formate
―
0.92
―
―
―
―
AMP
#
#
0.80
―
0.88
0.95
Glycogen
―
―
0.70
0.80
0.90
0.86
Adenine
―
-0.93
#
#
#
#
24
a Correlation
25
the concentrations, respectively. p=0.05, df (degree of freedom)=7, r=0.666 was used as the
26
corresponding cutoff value of correlation coefficient for the statistical significance based on the
27
discrimination significance. b The absolute value of correlation coefficient is less than cutoff value.
28
c
coefficient, positive and negative signs indicate positive and negative correlation in
The metabolite was not detected.
29
6