Table S3: Signature gene sequences from phages and primary isolates collected from the four cruises reported in this study
(A) DNA polymerase gene sequences from T7-like phages
#
Primary
isolate ID
9ecp
Accession
number
KM113820
Host
5e
7g
d67f2
c7e4
Syn
CC9605
Date
isolated
March
2010
Site of
isolation
Red Sea,
IUI pier, 0m
Details of phages and primary isolates with nearidentical sequences
11 phages or primary isolates
Above 99% identity to S-TIP67
Isolated on Syn CC9605 and Pro MED4
From March 2009, August 2009 and 2010 cruises
KM113811
Pro
MED4
March
2009
Red Sea,
IUI pier, 0m
KM113818
Pro
MED4
March
2009
Red Sea,
IUI pier, 0m
Pro
MED4
August
2010
Red Sea,
St. A, 20m
Syn
CC9605
August
2010
Red Sea,
IUI pier, 0m
Pro
MED4
August
2010
Red Sea,
St. A, 60m
4 phages or primary isolates
Above 99% identity to P-TIP42
Isolated on Pro MED4
From March 2009 cruise
7 phages or primary isolates
Above 97% identity to P-TIP44
Isolated on Pro MED4, MIT9215 and NATL2A
From March 2009, August 2009 and 2010 cruises
3 phages or primary isolates
Above 99% identity to P-TIP43
Isolated on Pro MED4 and MIT9215
From March 2009 and August 2010 cruises
3 primary isolates
Above 99% identity to dp8b1
Isolated on Syn CC9605 and Pro MED4
From August 2010
2 primary isolates
Above 99% identity to 7fdp3
Isolated on Pro MED4
From March 2009 cruise
Less than 97% identity to other sequences
KM113824
KM113823
6ed6p
KM113816
4f
KM113810
Near identical sequences to representative:
Phage ID – GenBank accession number
&#
S-TIP67 – KC507138
&#
10G – KC507119
#
4dc – KM113809
2fc6 – KM113808; 7dc6 – KM113817
6bc6 – KM113814; 9ec6 – KM113819
11ec6 – KM113822; 5gcp – KM113813
11bc6 – KM113821
&#
P-TIP42 – KC507139
6b – KM113815
5f – KM113812
P-TIP44 – KC507140
9f – KC507123
&#
5en – KC507121
&
&
P-TIP43 – KC507141
5d – KC507122
&
&
c8b4 – KC507105
dp8b1 – KC507106
&
&#
7fdp3 – KC507110
&
Pro
August Red Sea,
MIT9215 2010
St. A, 20m
#
Phage sequences used for treeing in Suppl. Fig. 1.
&
Phages and primary isolates collected during the four cruises reported here and presented previously in Dekel-Bird et al. (2013);
The S-TIP41 phage and 4dn primary isolate were isolated from these cruises and appear in Dekel-Bird et al. (2013) but are not shown in this table.
Three additional sequences were of low quality and were not included in this analysis.
Syn = Synechococcus, Pro = Prochlorococcus
B) Portal protein gene (g20) sequences from T4-like phages
#
Primary
isolate name
2Bnp
8G092
5Bnp
6Bnp
2Gdp
2B096
4B092
8B092
8B026
7Gmp
Accession
number
KM113830
KM113842
KM113835
KM113836
KM113831
KM113829
KM113833
KM113841
KM113840
KM113839
Host
Date isolated
Site of isolation
Pro NATL2A
Syn WH8109
Pro NATL2A
Pro NATL2A
Pro MED4
Syn WH8109
Syn WH8109
Syn WH8109
Syn WH8102
Pro MIT9215
March 2009
March 2009
August 09
March 2010
March 2010
March 2010
March 2010
March 2010
March 2010
August 2010
Red Sea, IUI pier, 0m
Red Sea, St. A, 20m
Red Sea, IUI pier, 0m
Red Sea, IUI pier, 0m
Red Sea, IUI pier, 0m
Red Sea, St. A, 60m
Red Sea, St. A, 20m
Red Sea, St. A, 20m
Red Sea, St. A, 60m
Red Sea, IUI pier, 0m
Details of phages and primary isolates with nearidentical sequences
Less than 97% identity to other sequences
Less than 97% identity to other sequences
Less than 97% identity to other sequences
Above 97% identity to P-SSM5
Less than 97% identity to other sequences
Less than 97% identity to other sequences
Less than 97% identity to other sequences
Less than 97% identity to other sequences
Less than 97% identity to other sequences
2 primary isolates with above 99% identity
Isolated on Pro MIT9215
From March 2009 cruise
Less than 97% identity to other sequences
Less than 97% identity to other sequences
Less than 97% identity to other sequences
Above 97% identity to P-SSM5
5Bd2
KM113834
Pro MED4
August 2010
Red Sea, St. A, 20m
7E02p
KM113837
Syn WH8102
August 2010
Red Sea, IUI pier, 0m
7G09p
KM113838
Syn WH8109
August 2010
Red Sea, IUI pier, 0m
4B09p
KM113832
Syn WH8109
August 2010
Red Sea, IUI pier, 0m
#
Phage sequences used for treeing in Suppl. Fig. 2.
One additional sequence was of low quality and was not included in this analysis; Syn = Synechococcus, Pro = Prochlorococcus
(C) DNA polymerase gene sequences from TIM5-like phages
#
Phage/primary
isolate name
S-TIM5
Accession
number
JQ245707
Host
S-TIM54
KM113825
Syn WH8102
6C
KM113826
Syn CC9605
9E
KM113827
Syn WH8102
7B
KM113828
Syn WH8102
#
Syn WH8102
Date
isolated
October
2008
Site of isolation
Details of primary isolates with identical DNA polymerase gene sequences
Red Sea,
IUI pier, 0m
10 primary isolates
Identical to the archetype phage
Isolated on Syn WH8102 and CC9605 hosts
From all 4 cruises
March
2009
March
2010
Red Sea
St. A, 20m
Red Sea,
IUI pier, 0m
March
2010
August
2010
Red Sea
St. A, 20m
Red Sea,
IUI pier, 0m
Phage sequences used for treeing in Suppl. Fig. 3.
Syn = Synechococcus
3 primary isolates
Isolated on Syn CC9605
From Aug 2009 and March 2010 cruises
52 primary isolates
Isolated on Syn WH8102 and CC9605 hosts
From all 4 cruises