The effect of NaCl(s) on grass Schreefel, R. & Eldewieh, S. Het 4e Gymnasium, The Netherlands Received April 2011 Summary Road salt is widely used in regions with cold winters in order to combat slippery roads and to avoid dangerous situations that can cause lethal accidents. This is the positive side of spreading salt. Some years there is more salt used than others according to the severity of the winter. The use of more salt there came negatives sides; road salt can cause for example corrosion on cars and motorcycles. And salt used in the winter solves in the water and ends up in the groundwater. It is widely known that salt is not very good for plants. That is why not all plants can grow close to the see because of the salt. This is why we wanted to study how big the effect of salt is on growing plants. © 2011 Schreefel&Eldewieh. All rights received Introduction During cold winters citizens expect the government to deice the road with as much salt as needed. But the governments do not spread to much salt, because it is very expensive and it can have environmental effects. It is widely known that salt isn’t good for plants and animals. However it is not clear how big the effect of the salt is. That is why we wanted to do our research about the effect of road salt on the environment. We hadn’t unfortunately the possibility to do our research on a big area. Therefore we have made a small preparation with grass and done our research about the effect of NaCl on the growth of grass. This had an advantage: we could control the amount of salt in the water and our results would not be affected by other variables. Experimental procedure and approach We took ten identical glass petri, all numbered, and each one filled with two filters with a layer of cotton wool above it. Then we put 30 seeds of grass (pokon grass repair set of Gamma) on the top layer of cotton wool. All the glass petri were then put down in a tray under a fluorescent tube. We made two lines with each five petri glasses. We had decided to give the bottom line from day one a NaCl-solution. The second line received water after 2 weeks. The different solutions that we have used are given in the following table: Number of the glass petri 1 2 Percentage of NaCl in the added water Only water 10gram/l NaCl 3 4 5 6 7 8 9 10 30gram/l NaCl 50 gram/l NaCl 100 gram/l NaCl Only water 10gram/l NaCl from week 2 30 gram/l NaCl from week 2 50 gram/l NaCl from week 2 100 gram/l NaCl from week 2 Table1: Different solutions of NaCl. We gave the seeds water every 3 or 4 days. Before we gave them the water we checked how far the seeds were with growing. We gave them each time 50 ml of the NaCl-solutions. After a while as we were working on our research we experienced that after the water was evaporated there remained a hard layer of salt. This layer grew every time we gave the seeds water. So we started again with the same approach but with new quantities of salt. The new solutions that we have used are given in the following table: Number of the glass petri 1 2 3 4 5 6 7 8 9 10 Percentage of NaCl in the added water Only water 1gram/l NaCl 3gram/l NaCl 5 gram/l NaCl 10 gram/l NaCl Only water 1gram/l NaCl from week 2 3 gram/l NaCl from week 2 5 gram/l NaCl from week 2 10 gram/l NaCl from week 2 Table 2: The new solutions of NaCl. Data gathering and analysis We looked every 3 or 4 days how the seeds grew and we wrote all our notations down. We wanted to use a ruler to measure the length of the grass. We also judged the developments with the eye. looked if dirt or loam can hold the water better than the cotton wool that we have used. We also think that it was unnecessary that we used a lamp for the seeds to germinate. Light is used for photosynthesis what seeds still don’t do. We still have not completed our research. We can see what we have done till now as information to build on and do other tests. We have come with a new preparation that we can see as our conclusion. It will look like the picture beneath. Figure 1: The layer of salt. Results After four weeks of waiting and giving the plants water we came with the following results: grass seeds cannot grow well in an environment with water containing 1 gram of salt per liter of water. We also concluded that not every single seed germinates. Conclusion and discussion When we look back at our results we can induce that our approach has not worked well. We have seen that we have used a NaClsolution with too big amounts of salt. As results there arose a layer of salt on the seeds. Our first set of grass seeds did not grow, so we must started again with lower salt percentage in the water. We discovered that the new amount of salts in the solutions are also too much. Another problem was that the water in the NaCl-solution evaporated so quickly that we had to refill the glass petri every day. That was for us a huge problem. If we gave the seeds to much water, so we had not refill it so much, they drowned. And we did not know how bad it is for seeds to have no air. We also know now that not every seed that you plant will germinates and become a blade of grass. It may be due to the type of seeds that we have used, but it could also be the place it was lie. The cotton wool that we have used was not perfectly equal. This caused that seeds in the deeper parts got more water what can possibly ensure that they grow better that other-placed seeds. What we could have done was to look if loam was a better option to use, because in the nature seeds grow in dirt. Next time we also Figuur 2 New preparation We will cut a coffee filter from one side so we can see inside it. We will hang the filter up and it will be connected with the different NaClsolution by two or more strips. This always keeps the seeds wet and with this we will be avoiding one of our problems. We will also plant new seeds in loam and to see how big the effect of salt on grass seeds and the environment is and to come with clear figures. The big questions about road salt will still be unanswered. How big is the effect of salt on a bigger scale? And how different is it in different countries? Some researchers have tried to find an answer on it. And will there ever a substance developed that can replays salt? The time will reveal the answers… Bibliography 1. http://onlinepubs.trb.org/onlinepubs/sr/sr2 35/069-082.pdf 2. http://www.sciencedirect.com/science?_o b=ArticleURL&_udi=B6V733XXCY2NG&_user=10&_coverDate=01%2F01%2F 2000&_rdoc=1&_fmt=high&_orig=gatew ay&_origin=gateway&_sort=d&_docanch or=&view=c&_searchStrId=1717282279 &_rerunOrigin=scholar.google&_acct=C 000050221&_version=1&_urlVersion=0 &_userid=10&md5=f098fd8341a6655cbd e25edb42f0c74b&searchtype=a 3. http://nl.wikipedia.org/wiki/Strooizout