Supporting information:
Table S1
Table S1. Repeated-measures analysis of variance (profile analysis) on photosynthetic parameters determined on the first and ninth day of the experiment in
the leaves of P. coleoides plants. Experimental conditions were as described in Table 1. D.f. are shown in the brackets (first number represents d.f. of main
effects and their interactions and the second number is d.f. of error). Other abbreviations are explained in Table 2.
Source
Between-subjects
A (d.f.: 1; 12)
gs (d.f.: 1; 12)
F
F
P>F
P>F
Fq'/Fm' (d.f.:1;12)
F
P>F
ETR (d.f.: 1; 12)
F
P>F
NPQ (d.f.: 1; 12)
ci (d.f.: 1; 12)
F
P>F
F
P>F
228.67
< 0.0001
10.02
0.0081
PAR
51.37 < 0.0001
28.47
0.0002
1383.65
< 0.0001
UV-B
17.68
0.0012
36.55 < 0.0001
0.70
0.4185
0.14
0.7100
8.40
0.0134
6.77
0.0231
1.60
0.2302
3.09
0.1040
1.64
0.2239
7.98
0.0153
2.08
0.1746
PAR × UV-B
3.36
0.0917
Fq'/Fm' (d.f.:1; 12)
2.93
0.1124
166.40 < 0.0001
Within-subjects
Day
A (d.f.: 1; 12)
17.58
0.0012
gs (d.f.: 1; 12)
5.87
0.0321
ETR (d.f.: 1; 12)
3.41
0.0898
NPQ (d.f.: 1; 12)
1.76
0.2094
ci (d.f.: 1; 12)
0.12 0.7330
Day × PAR
12.52
0.0041
0.06
0.8175
2.84
0.1180
3.38
0.0907
0.05
0.8192
0.02
0.8982
Day × UV-B
0.90
0.3620
1.49
0.2460
0.24
0.6350
0.17
0.6867
0.36
0.5621
0.19
0.6698
Day × PAR × UV-B
1.01
0.3340
0.32
0.5819
2.76
0.1224
0.74
0.4058
1.86
0.1974
0.57
0.4655
Table S2
Table S2. Two-way ANOVA results for the effects of UV-B radiation exposure (0.9 W m−2) and two PAR intensities (LL:
395 μmol m−2 s−1 and HL: 1350 μmol m−2 s−1) and their interactions on the pigment contents and ratios in the leaves of P.
coleoides plants. Experimental conditions were the same as in Table 1. D.f. are shown in the brackets (first number
represents d.f. of main effects and their interactions and the second number is d.f. of error). Other abbreviations are
explained in Table 3.
Source of
Source of
Trait
F
P>F
Trait
F
P>F
variation
variation
UV-B
2.96
0.1108
UV-B
1.77
0.2086
Chl a
β-Carotene
(d.f.=1;16)
(d.f.=1;16)
PAR
2.46
0.1427
PAR
20.84
0.0006
UV-B × PAR
0.03
0.8731
UV-B × PAR
0.04
0.8485
Chl b
(d.f.=1;16)
UV-B
PAR
UV-B × PAR
4.58
26.95
0.16
0.0536
0.0002
0.6918
Neoxanthin
(d.f.=1;16)
UV-B
PAR
UV-B × PAR
4.49
17.91
0.14
0.0557
0.0012
0.7160
Chl (a+b)
(d.f.=1;16)
UV-B
PAR
UV-B × PAR
2.73
1.81
0.02
0.1244
0.2035
0.8989
Lutein
(d.f.=1;16)
UV-B
PAR
UV-B × PAR
6.84
13.69
0.12
0.0226
0.0030
0.7306
Chl (a/b)
(d.f.=1;16)
UV-B
PAR
UV-B × PAR
3.12
0.21
1.92
0.1025
0.6572
0.1901
Violaxanthin
(d.f.=1;16)
UV-B
PAR
UV-B × PAR
3.81
4.28
0.20
0.0745
0.0608
0.6609
Chl (a+b)/Σcar
UV-B
PAR
UV-B × PAR
0.31
123.12
1.83
0.5872
< 0.0001
0.2014
Table S3
Table S3. Three-way nested ANOVA results for the effects of tissue type (chlorophyllous and non-chlorophyllous tissues), UV-B
radiation exposure and two PAR intensities as well as their interaction on the content of phenolic compounds found in the leaves of
P. coleoides plants. Experimental conditions were as described in Table 1. D.f. are shown in the brackets (first number represents d.f.
of main effects and their interactions and the second number is d.f. of error). HBA: total hydroxybenzoic acid; SyA: syringic acid;
CA: caffeic acid, RA: rosmarinic acid; Cat: catechin; ECat: epicatechin; Ap: apigenin; Cy: cyanidin.
Trait
Source of variation
F
P>F
SyA
(d.f.: 1; 56)
Trait
Source of variation
tissue
UV-B
PAR
tissue × UV-B
tissue × PAR
UV-B × PAR
tissue × UV-B × PAR
240.61
0.02
5.86
1.01
0.39
1.50
0.33
HBA
(d.f.: 1; 56)
tissue
UV-B
PAR
tissue × UV-B
tissue × PAR
UV-B × PAR
tissue × UV-B × PAR
CA
(d.f.: 1; 56)
tissue
UV-B
PAR
tissue × UV-B
F
P>F
< 0.0001
0.8947
0.0188
0.3192
0.5367
0.2252
0.5693
Cat
(d.f.: 1; 56)
tissue
UV-B
PAR
tissue × UV-B
tissue × PAR
UV-B × PAR
tissue × UV-B × PAR
78.36
1.59
0.57
0.07
10.05
0.08
4.80
< 0.0001
0.2124
0.4535
0.7969
0.0025
0.7777
0.0327
120.17
3.19
44.48
0.64
2.92
12.10
0.76
< 0.0001
0.0796
< 0.0001
0.4281
0.0929
0.0010
0.3857
ECat
(d.f.: 1; 56)
tissue
UV-B
PAR
tissue × UV-B
tissue × PAR
UV-B × PAR
tissue × UV-B × PAR
497.66
5.26
40.67
11.70
1.87
3.28
0.15
< 0.0001
0.0255
< 0.0001
0.0012
0.1774
0.0756
0.6991
329.13
0.34
10.61
1.19
< 0.0001
0.5616
0.0019
0.2793
Ap
(d.f.: 1; 56)
tissue
UV-B
PAR
tissue × UV-B
7.64
93.52
101.85
0.03
0.0108
< 0.0001
< 0.0001
0.8549
RA
(d.f.: 1; 56)
tissue × PAR
UV-B × PAR
tissue × UV-B × PAR
5.84
11.01
8.04
0.0190
0.0016
0.0064
tissue
UV-B
PAR
tissue × UV-B
tissue × PAR
UV-B × PAR
tissue × UV-B × PAR
493.41
15.62
55.17
1.88
7.50
10.08
0.85
< 0.0001
0.0002
< 0.0001
0.1761
0.0083
0.0024
0.3597
Cy
(d.f.: 1; 56)
tissue × PAR
UV-B × PAR
tissue × UV-B × PAR
7.00
15.46
0.07
0.0142
0.0006
0.7976
tissue
UV-B
PAR
tissue × UV-B
tissue × PAR
UV-B × PAR
tissue × UV-B × PAR
21.98
36.63
116.13
13.80
14.08
16.38
8.15
< 0.0001
< 0.0001
< 0.0001
0.0011
0.0010
0.0005
0.0087
Table S4
Table S4. Three-way nested ANOVA results for the effects of tissue type (chlorophyllous and non-chlorophyllous), UV-B
radiation exposure (0.90 W m−2) and two PAR intensities (LL: 395 µmol m−2 s−1 and HL: 1350 µmol m−2 s−1) and their
interactions on Asc content, APX and POD activities in the leaves of P. coleoides plants. Experimental conditions were as
described in Table 1. D.f. are shown in the brackets (first number represents d.f. of main effects and their interactions and the
second number is d.f. of error). Other abbreviations are explained in Fig. 4.
Trait
Asc
(d.f. = 1; 40)
APX
(d.f. = 1; 40)
Source of variation
tissue
UV-B
PAR
tissue × UV-B
tissue × PAR
UV-B × PAR
tissue × UV-B × PAR
F
P>F
60.36 < 0.0001
2.83
0.1004
16.02
0.0003
1.05
0.3122
24.43 < 0.0001
11.54
0.0016
0.62
0.4369
tissue
UV-B
PAR
tissue × UV-B
tissue × PAR
UV-B × PAR
tissue × UV-B × PAR
0.01
19.37
4.87
1.39
2.02
1.54
11.68
0.9875
0.0001
0.0357
0.2483
0.1659
0.2248
0.0020
Trait
POD
(d.f. = 1; 52)
Source of variation
tissue
UV-B
PAR
tissue × UV-B
tissue × PAR
UV-B × PAR
tissue × UV-B × PAR
F
P>F
33.75 < 0.0001
33.49 < 0.0001
23.13 < 0.0001
4.99
0.0326
10.10
0.0033
6.48
0.0159
0.01
0.9469