) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
University of Baghdad
College Name
College of Sciences
Department
Dept. of Biology
Full Name as written
in Passport
Dimah Nazar Faraj Passat
e-mail
Dimahpassat2005@yahoo.com
Career
Assistant Lecturer
Master
Lecturer
Assistant Professor
Professor
PhD
Thesis Title
The effect of aqueous & alcoholic extracts of withania somnifera (L.) Dun. &
Urtica urens (L.) in some virulence factors & plasmid DNA in local isolates of
Pseudomonas aeruginosa
Year
2006
Abstract
Pseudomonas aeruginosa was isolated from various clinical specimens including :
wound , burn , ear , urine and blood , collected from patients hospitalized in Yarmouk
, Al-Kindy and Al-Elweia for children hospital,
A total of (50) (out of 110) isolates were obtained of which (44 %) were isolated
from wounds , (20 %) from urine and ear infections , (12 %) from burns and (4 %)
from blood specimen.
Antibiotic resistant tests have indicated that (68 %) of P. aeruginosa isolates were
multiresistant to (6 – 9) antibiotics and (26 %) were resistant to (1 – 5) antibiotics
and (6 %) were resistant to (10 – 12) antibiotics , P. aeruginosa isolates exhibited
(33) antibiogram patterns.
Detection of some virulence factors production by these isolates indicated that
(82 %) were producers of hemolysin, (74 %) were elastase producers and (66 %)
were urease producers. The group producing all three virulence factors was
dominant.
Two isolates were selected , the first isolate (PD1) was resistant to (11)
antibiotics , and produced hemolysin , elastase and urease , the second isolate (PD2)
was resistant to (4) antibiotics only (out of 12) and produced hemolysin , elastase but
not urease.
Screening for plasmid DNA indicated that the resistant isolate (PD1) had a single
plasmid , while the sensitive isolate (PD2) had no plasmid.
Minimum inhibitory concentration (MICs) of the aqueous and ethanolic extracts
of both (whole) plants Withania somnifera (L.) Dun. and Urtica urens L., were
determined towards P. aeruginosa (PD1,PD2), Results obtained showed that MIC of
ethanolic extract of W. somnifera was (5) mg / ml and of aqueous extract was (175)
mg / ml. The MIC for the ethanolic extract of U. urens was (25) mg / ml , while the
aqueous extract had no inhibitory effect towards P. aeruginosa up to (175) mg / ml.
The effect of Sub – MICs of the aqueous and ethanolic extracts of both plants on
the production of hemolysin , elastase and urease was investigated. Sub-MICs of the
ethanolic and aqueous extract of W. somnifera had an inhibitory effect on hemolysin
production and a suppressing effect on elastase but no effect on urease production at
100 , 125 , 150 mg / ml for the aqueous extract and 2 , 3 , 4 mg / ml for the ethanolic
extract.
) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
Sub-MICs of the aqueous and ethanolic extract of U. urens had an inhibitory effect
on elastase production and a suppressing effect on hemolysin , but no effect on
urease production at 125 , 150 , 175 for the aqueous extract and 10 , 15 , 20 mg / ml
for the ethanolic extract.
The electrophoresis of plasmid DNA isolated from bacterial cell treated with
plant extracts indicated that neither W. somnifera nor U. urens extracts had a curing
effect on the plasmid at sub-MIC concentrations for PD1.
Vising infrared spectroscopy spectrum showed that the sub MICs of ethanolic and
aqueous extracts of . W. somnifera (4 mg / ml , 150 mg / ml) made an alteration in
NH+3 groups at wave band (2000-3100) cm-1 and C=O group at 1649 cm-1 , while the
Sub-MICs of the ethanolic and aqueous extracts of U. urens (20 mg / ml , 175 mg / ml)
made changes in NH+3 , C=O and PO2- groups at the wave bands (2000-3100) , 1649 ,
690-810 cm-1 respectively. These results indicate the possible effect of plant extracts
on the conformation of the DNA molecules affecting some of its biological functions.