Induction of embryo-like structures by liquid culture in mulberry (Morus
alba L)
By:Shajahan, A (Shajahan, A); Kathiravan, K (Kathiravan, K); Ganapathi, A (Ganapathi, A)
BREEDING SCIENCE
Volume: 45
Issue: 4
Pages: 413-417
Published: DEC 1995
View Journal Information
Abstract
Suspension cultures of calli derived from hypocotyl explant of Morus alba L. var. MR(2) have produced embryo like
structures. Calli were induced on the semisolid MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid
(2,4-D) and 0.5 mg/l 6-benzylaminopurine (BAP). They were transferred to MS liquid medium with different
concentrations (0.1-1.0 mg/l) of 2,4-D and further subcultured at weekly intervals into fresh medium. Embryo-like
structures were observed at each stage of development. Cells in the suspension culture having enriched cytoplasm with
prominent nuclei, divided to form filamentous structures and continuous division of these structures lead to the
development of globular and heartshaped structures, Suspension medium containing 0.5 mg/l 2,4-D was essential to
induce high frequencies of embryo like structures while lower (0.1-0.2 mg/l) and higher (1.0 mg/l) concentrations caused
either root or callus formation respectively. There was no further development of the heartshaped structure in the liquid
medium containing 2,4-D.
Keywords
Author Keywords:Morus alba; hypocotyl; suspension culture; 2,4-D; embryo-like structure
KeyWords Plus:SOMATIC EMBRYOGENESIS; PLANT-REGENERATION; CALLUS-CULTURES
Author Information
Reprint Address: Shajahan, A (reprint author)
BHARATHIDASAN UNIV, DEPT BIOTECHNOL, TIRUCHCHIRAPPALLI 620024, INDIA.
Publisher
JAPANESE SOC BREEDING, UNIV TOKYO, GRADUATE SCHOOL AGRICULTURAL LIFE SCIENCES, BUNKYO-KU,
TOKYO, 113-8657, JAPAN
Categories / Classification
Research Areas:Agriculture; Plant Sciences
Web of Science Categories:Agronomy; Plant Sciences
Document Information
Document Type:Article
Language:English
Accession Number: WOS:A1995TP48000001
ISSN: 1344-7610
eISSN: 1347-3735
Other Information
IDS Number: TP480
Cited References in Web of Science Core Collection: 20
Times Cited in Web of Science Core Collection: 2