L. monocytogenes has a broad effect on transcription in Drosophila melanogaster
While previous microarrays have been done in adult flies with L. monocytogenes, this is the first microarray
performed with this parental strain, w1118. As parental lines respond differently to infection, looking at genes
changing in this line were necessary to adequately compare changes caused by an interruption in ets21c.
We looked at parental line changes in two contexts. First, we looked for any gene that changed during L.
monocytogenes infection compared to unmanipulated flies, including genes induced through wounding and media
injection. Second, we specifically selected for genes which changed during L. monocytogenes infection, but not
during wounding. Both of these classes are important for the immune response to and eventual survival during
infection, but considering them separately allows for proper interpretation.
Looking at all changes during infection, 232 genes were significantly different between unmanipulated and L.
monocytogenes infected flies (p<0.01). Gene Ontology (GO) analysis revealed many expected categories including
humoral immune response, defense response, and the Toll signaling pathway. There was also enrichment in a
number of metabolic categories: carbohydrate metabolism, electron transport, hexose metabolism, lipid metabolism,
monosaccharide metabolism, pigment metabolism, protein metabolism, and secondary metabolism. A list of the
genes with changes greater than 4.5 fold is in Supplemental Table 2.
After filtering out genes affected by wounding, we found L. monocytogenes infection caused changes in 146 genes
listed in Supplemental Table 3. This list was generated by selecting genes which significantly changed between
unmanipulated and L. monocytogenes infected flies (p<0.01) and removing any genes which were significantly
different between unmanipulated and media injected flies (p<0.1). Gene Ontology analysis revealed similar results
to those above, expected immune clusters – humoral immune response, defense response, and Toll signaling
pathway – and metabolic clusters – carbohydrate metabolism, electron transport, lipid metabolism and pigment
metabolism.
A closer look at regulated immunity genes reveals that Toll signaling genes are up-regulated 2-4 fold during L.
monocytogenes infection. These genes are not significantly induced by media injection, but the downstream readouts of the pathways, anti-microbial peptides, are. The induction of these genes indicates activation of both the imd
and the Toll pathways. Gregorio et al showed that the Toll components that we see up-regulated (cactus, pelle, Toll,
spaetzle, and necrotic) as well as additional factors like relish and frost are all spaetzle signaling dependent.[1] Of
the anti-microbial peptides significantly induced, attacin-A, attacin-B, drosomycin, and metchnikowan can be
induced by either Toll or imd; attacin-C and diptercin B require imd signaling; and defensin requires signaling
through both toll and imd pathways.[1] L. monocytogenes is gram-positive bacterium that excites both the Toll and
imd pathways and yet L. monocytogenes possesses a DAP-type peptidoglycan which, according to Drosophila
dogma, should only cause signaling through the imd pathway.
In the genes significantly down-regulated during L. monocytogenes infection, there is significant enrichment in
metabolic pathways. Transcripts in the glycolysis/gluconeogenisis, beta-oxidation, mitochondrial fatty acid
metabolism and the pentose-phosphate pathway are depressed during infection. This could be indicative either of
loss of substrates or of a regulatory shift on the fly’s part in an effort to regulate energy usage.
Miscellaneous other genes of interest include frost, which affects cold resistance and is transcriptioanlly induced
during other infections, and adipokinetic hormone receptor, which is important for triglyceride utilization.[1-3]
Et21c mutant flies have few baseline significant differences from parental
To select for genes that are different in ets21c mutants in the absence of infection we compared unmanipulated
parental to unmanipulated ets21c mutants (Supplementary Table 4). There were 34 genes which had significantly
different basal expression levels between the ets21c mutant and parental line (p<0.01), and this gene list was
enriched for genes in the defense response: lysozyme X, drosomycin-4, immune induced molecule 23, ebony,
Ugt86dD, CG31832, and CG16799.
Ets21c mutant flies induce fewer genes during L. monocytogenes infection
L. monocytogenes infection of ets21c piggybac mutants caused significant changes in 41 genes (p<0.01) and only 18
of these genes do not change during media injection as compared to the 236 and 141 genes changes under the same
conditions, respectively, in the parental line (Supplementary Table 5). Gene Ontology analysis revealed enrichment
in humoral immune response, defense response and toll signaling pathway.
Notably missing, compared to the
parental results, was enrichment in the metabolic systems. There was enrichment in carbohydrate catabolism, but
this is a distinct GO group from those seen in the parental line.
To look at which genes are induced during parental infection but not during ets21c mutant infections, we took the
list of genes significantly changed between unmanipulated and L. monocytogenes infected in the parental line
(p<0.01), selected against genes changed through media injection (p<0.1), and selected against genes changed in
ets21c mutants (p<0.1). We used higher p-values for our anti-selection criteria to have the strictest criteria for gene
selection.
The anti-selection of both media injection and ets21c was mostly redundant, with the ets21c selection criteria only
removing two additional genes. To loosen selection criteria, we looked at all genes which change in the parental
line during L. monocytogenes infection (unmanipulated versus L. monocytogenes, p<0.01) and filtered for genes
which changed during ets21c piggybac infection (unmanipulated vs. L monocytogenes, p<0.1). This criterion
eliminated 41 genes, leaving a list of 191 genes which were not affected by infection in the ets21c mutant
(Supplementary Table 2). These genes almost entirely coincide with genes affected by media injection, hence the
low level of additional selection by introducing both restrictions.
The gene most highly induced during L. monocytogenes infection in the parental line, but not in the ets21c mutant
and during wounding, was wntD. Mutants in wntD are susceptible to L. monocytogenes infection, making this a
potential mediator of ets21c’s immune phenotype.
One of the genes most down-regulated during infection in the parental line, but not affected in the ets21c mutant, is
the gene adipokinetic hormone receptor (akhr). Akhr is the Drosophila equivalent of the mammalian glucagon
receptor and is responsible to controlling the release of triglyceride stores.[2,3]
Ets21c mutants have a different expression signature during L. monocytogenes infection
Because there are so few changes induced in the ets21c mutants by L. monocytogenes infection, we also looked at a
direct comparison between L. monocytogenes infected ets21c mutant versus infected parental. When we did this
comparison, 124 genes were significantly different (p<0.01) (Supplementary Table 6). This revealed both genes
induced during infection and baseline differences, when we selected against the baseline differences in
unmanipulated flies (p<0.1) we reduced the number to 94 genes. GO analysis of the non-baseline differences were
enriched in catabolism, electron transport, iron binding, oxidoreductase activity, and purine nucleotide metabolism.
This selection condition again highlights WntD and akhr as potential mediators of the ets21c phenotype. This
criterion also highlights the gene frost, which is strongly induced in the parental line during infection (~20 fold) but
is only induced four-fold in the ets21c mutant. As mentioned above, frost affects cold tolerance in Drosophila, and
is known to be immune induced by a number of microbes.[4-6]
Supplemental Table 2. Genes regulated during L. monocytogenes infection in parental line.
Gene Name
CG
CG4950
CG16756
female-specific independent of
transformer
Cytochrome b5-related
Adipokinetic hormone receptor
Cyp4ac2
CG6067
CG31778
Lipid storage droplet-1
CG8147
CG2736
Retinoid- and fatty-acid binding
protein
CG4716
Glutactin
CG9466
CG5428
lectin-33A
CG15092
nicotinic acetylcholine receptor beta
21C
Hemolectin
Papilin
CG3597
CG10131
Phosphoribosylamidotransferase 2
CG14990
CG4716
CG9468
CG13877
CG13607
regucalcin
CG9837
Sorbitol dehydrogenase 1
CG6503
CG18249
CG5288
Serine pyruvate aminotransferase
CG15199
PGRP-SB1
Activin Like Protein at 23B
Attacin-C
CG4950
CG16756
CG17820
Significant change
during media
injection?
Significant change in
ets21c mutant during
infection?
-15.68
-15.12
-14.2
*
*
*
*
CG13279
CG11325
CG17970
CG6067
CG31778
CG10374
CG8147
CG2736
CG11064
-13.67
-12.06 / -2.98
-10.5
-10.04
-9.53
-7.92
-7.91
-7.79
-7.05
*
*/
*
CG4716
CG9280
CG9466
CG5428
CG16834
CG15092
CG11822
-6.74
-6.44
-6.32
-6.29
-6.15
-6.04
-5.71
*
CG7002
CG33103
CG3597
CG10131
CG10078
CG14990
CG4716
CG9468
CG13877
CG13607
HDC18536
CG9837
CG1982
CG6503
CG18249
CG5288
CG3926
CG15199
CG9681
CG16987
CG4740
Fold Change
(Unmanip vs.
Listeria)
-5.59
-5.54
-5.47
-5.36
-5.2
-5.16
-5.16
-5.14
-5.11
-5
-4.99 / -2.59
-4.87
-4.68
-4.64
-4.61
-4.58
-4.56
-4.52
4.57
4.6
4.62
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*/
*
*
*
*
*
*
*
*
Supplemental Table 2. Genes regulated during L. monocytogenes infection in parental line (continued).
Gene Name
PGRP-SB1
Activin Like Protein at 23B
Attacin-C
CG9542
CG1299
Ser7
CG14205
PGRP-SC2
CG1102
Immune induced molecule 1
CG6639
CG4267
CG32373
Thiolester containing protein I
CG9080
Immune induced molecule 10
DiptericinB
CG2444
CG6361
CG5527
Attacin-B
CG6553
CG8791
CG11413
PGRP-SD
CG6687
Attacin-A
CG9989
CG14516
yellow-f
CG18563
CG33307
wnt inhibitor of Dorsal
CG2217
CG30080
CG30080
Frost
suppressor of white-apricot
CG14567
Immune induced molecule 23
CG11459
CG6675
CG31775
CG
Number
CG9681
CG16987
CG4740
CG9542
CG1299
CG2045
CG14205
CG14745
CG1102
CG18108
CG6639
CG4267
CG32373
CG18096
CG9080
CG18279
CG10794
CG2444
CG6361
CG5527
CG18372
CG6553
CG8791
CG11413
CG7496
CG6687
CG10146
CG9989
CG14516
CG18550
CG18563
CG16887
CG8458
CG2217
CT32157
CG30080
CG9434
CG3019
CG14567
CG15066
CG11459
CG6675
CG31775
Fold Change
4.57
4.6
4.62
4.64
4.67
4.83
5.09
5.16
5.22
5.33
5.49
5.73
5.74
5.77
5.85
6.01
6.31
6.54
6.6
6.63
6.79
6.88
7.2
7.87
8.35
8.71
8.99
9.13
9.56
11.46
11.85
12.52
17.37
17.38
17.86
18.63
23.21
26.48
33.71
37.39
37.56
42.41
70.51
Significant
change during
media
injection?
Significant
change in ets21c
mutant during
infection?
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
Supplemental Table 3. Genes regulated by L. monocytogenes infection but not wounding.
Gene Name
CG
Number
CG6067
CG2736
CG4716
CG9280
CG7002
CG10131
Fold
Change
-10.04
-7.79
-6.74
-6.44
-5.59
-5.36
CG10078
CG4716
-5.2
-5.16
CG13877
CG13607
CG6503
CG5288
Serine pyruvate
aminotransferase
CG15199
CG14400
lectin-24Db
CG10361
Cytochrome P450-18a1
Ance-4
CG2801
Open rectifier
K<up>+</up> channel 1
CG9295
ade5
CG10621
CG13082
sex-specific enzyme 2
CG32072
CG5958
Collagen type IV
Cyp28d1
CG33047
CG13877
CG13607
CG6503
CG5288
CG6067
CG2736
CG4716
Glutactin
Hemolectin
CG10131
Phosphoribosylamidotransferase 2
CG4716
Gene Name
wnt inhibitor of Dorsal
CG14516
CG8791
CG2444
CG1102
CG14205
CG
Number
CG8458
CG14516
CG8791
CG2444
CG1102
CG14205
Fold
Change
17.37
9.56
7.2
6.54
5.22
5.09
CG1299
CG9542
4.67
4.64
-5.11
-5
-4.64
-4.58
CG1299
CG9542
Activin Like Protein at
23B
CG1698
CG4618
CG17350
CG16987
CG1698
CG4618
CG17350
4.6
4.42
4.39
4.26
CG3926
CG15199
CG14400
CG2958
CG10361
CG6816
CG8196
CG2081
-4.56
-4.52
-4.33
-4.33
-4.21
-4.16
-4.07
-4.03
CG31617
cactus
CG11843
CG32412
CG18530
Defensin
CG3505
p115
CG31617
CG5848
CG11843
CG32412
CG18530
CG1385
CG3505
CG1422
4.26
4.15
3.98
3.94
3.88
3.84
3.79
3.75
CG1615
CG9295
CG3989
CG10621
CG13082
CG4979
CG32072
CG5958
CG4145
CG10833
CG33047
-3.99
-3.97
-3.89
-3.79
-3.74
-3.65
-3.63
-3.62
-3.53
-3.52
-3.49
CG11992
CG1689
CG11331
CG10697
CG7219
CG13075
CG6134
CG17119
CG1859
CG6375
CG34037
3.73
3.72
3.71
3.67
3.62
3.56
3.55
3.49
3.45
3.4
3.39
CG6034
Cyp6t1*
Iris
CG9497*
Glycerol 3 phosphate
dehydrogenase
Cytochrome P450-4d1
CG33493
Ser8
CG11407
CG6034
CG1644
CG4715
CG9497
-3.45
-3.45
-3.42
-3.25
Relish
lozenge
Serpin-27A
Dopa decarboxylase
CG7219
CG13075
spatzle
CG17119
Spn43Ad
pitchoune
CG34037
Suppressor of cytokine
signaling at 36E
CG6388
CG8913
nop5
CG15154
CG6388
CG8913
CG10206
3.23
3.21
3.21
3.12
CG9042
CG3656
CG33493
CG4812
CG11407
-3.25
-3.24
-3.1
-3.07
-3.06
CG5773
CG4927
CG9358
CG1607
CG5246
3.04
3.02
2.99
2.88
2.88
CG14523
CG14523
-3.05
CG5773
CG4927
Pherokine 3
CG1607
CG5246
PDGF- and VEGFrelated factor 2
CG13780
2.88
Supplemental Table 3. Genes regulated by L. monocytogenes infection but not wounding (continued).
Gene Name
CG
Number
CG5154
Fold
Change
-3.04
CG11325
CG3523
CG1112
CG11892
CG9511
-2.98
-2.97
-2.92
-2.88
-2.86
CG32158
Trypsin
acyl-Coenzyme A oxidase at
57D distal
CG15203
CG4019
pugilist
CG16926
CG14375
CG15096
CG32158
CG12385
-2.82
-2.82
CG9709
CG15203
CG4019
CG4067
CG16926
CG14375
CG15096
-2.81
-2.8
-2.8
-2.8
-2.77
-2.76
-2.74
CG33120
Cyp9h1
CG18301
Rhythmically expressed gene
2
Trehalose-6-phosphate
synthase 1
CG15406
fructose-1,6-bisphosphatase
CG33120
CG17577
CG18301
CG14823
CG7322
CG6206
CG8654
CG9510
Olfactory-specific 9
CG11315
Senescence marker protein-30
real-time
CG5560
CG11314
CG32667
Cyp6g1
Odorant-binding protein 99c
Imaginal disc growth factor 5
Adipokinetic Hormone
receptor
CG3523
Esterase-7
CG11892
CG9511
Gene Name
CG
Number
CG9441
Fold
Change
2.87
CG10627
CG11034
CG12772
CG9616
Urate oxidase
Cyclic-AMP response
element binding protein A
pelle
CG10627
CG11034
CG12772
CG9616
CG7171
2.83
2.81
2.74
2.74
2.7
CG7450
CG5974
2.68
2.63
CG18525
CG31686
CG17818
CG12116
CG3385
CG15044
CG9809
2.62
2.61
2.59
2.57
2.57
2.56
2.56
-2.73
-2.72
-2.69
Serine protease inhibitor 5
CG31686
rdgB
CG12116
nervy
CG15044
CG9809
Arginine
methyltransferase 8
CDC45L
CG31326
CG16840
CG3658
CG31326
2.54
2.51
2.48
CG3200
-2.69
CG7115
CG7115
2.46
CG4104
CG15406
CG31692
-2.69
-2.64
-2.64
CG3781
CG5550
CG18348
2.45
2.42
2.41
CG14823
CG7322
CG6206
CG8654
CG33085
CG10658
CG11315
CG7390
CG9528
CG5560
CG11314
CG32667
CG8453
CG7584
-2.6
-2.59
-2.56
-2.54
-2.53
-2.53
-2.49
-2.47
-2.46
-2.39
-2.37
-2.34
-2.3
-2.28
CG3781
CG5550
CG18348
Misexpression suppressor
of KSR 4
CG33258
CG9416
CG15043
CG31447
CR33258
CG9416
CG15043
2.4
2.36
2.35
2.19
Punch
Supplemental Table 4. Genes that are differentially expressed at baseline between ets21c mutants and the
parental line.
Gene Name
CG5139
Cyp6a17
CG18066
CG5381
Larval serum protein 2
CG31832
CG4409
Lysozyme X
Larval serum protein 1α
insulin-like peptide 5
CG14115
CG31672
Cyp9h1
CG16704
CG14499
ebony
Larval serum protein 1β
CG16799
CG11294
CG11893
Cyp6a23
Insulin-related peptide
drosomycin-4
CG10550
CG3397
Immune induced molecule 23
CG11909
CG32036
CG7900
Ugt86Dd
Syt7
Esterase-2
CG13313
CG13155
CG number
CG5139-RA
CG10241-RA
CG18066-RA
CG5381-RA
CG6806-RA
CG31832-RA
CG4409-RA
CG9120-RA
CG2559-RA
HDC09365
CG14115-RA
CG31672-RA
CG17577-RA
CG16704-RA
CG14499-RA
CG3331-RA
CG4178-RA
CG16799-RA
CG11294-RA
CG11893-RA
CG10242-RA
CG8167-RA
CG32282-RA
CG10550-RB
CG3397-RA
CG15066-RA
CG11909-RA
CG32036-RB
CG7900-RA
CG6633-RA
CG2381-RB
CG2505-RA
CG13313-RA
CG13155-RA
Fold Change
-33.21
-28.9
-14
-11.23
-7.4
-6.91
-5.87
-4.77
-4.17
-4.06
-3.56
-3.4
-3.07
-3.04
-2.63
-2.33
-2.26
-2.25
2.56
2.58
2.8
2.87
3.03
3.24
3.62
4.15
4.69
4.94
5.06
5.29
6.15
6.55
6.89
75.97
Supplemental Table 5. Genes regulated during L. monocytogenes infection in ets21c mutants.
Gene Name
CG4950
regucalcin
neither inactivation nor afterpotential D
CG34020
CG12374
CG11594
CG9631
Relish
CG15021
Cyp309a1
CG17325
Mec2
Diptericin B
CG13422
Immune induced molecule 10
Attacin-B
CG3505
NAD-dependent
methylenetetrahydrofolate dehydrogenase
CG17032
CG6553
CG5527
Thiolester containing protein II
CG6385
CG6687
PGRP-SC2
Immune induced molecule 10
CG11413
CG30080
CG31495
CG30080
Peptidoglycan recognition protein SA
yellow-f
CG4267
PGRP-SB1
CG32185
CG6361
CG13077
Immune induced molecule 23
PGRP-SD
CG2217
CG9989
Accession
CG4950-RA
HDC18536
CG31783-RA
Dm.2R.20325.0
CG12374-RA
CG11594-RC
CG9631-RA
CG11992-RA
CG15021-RA
CG9964-RA
CG17325-RA
CG7635-RA
CG10794-RA
CG13422-RA
CG33470
CG18372-RA
CG3505-RA
CG18466-RB
CG17032-RA
CG6553-RA
CG5527-RA
CG7052-RA
CG6385-RA
CG6687-RA
CG14745-RA
CG18279-RB
CG11413-RA
CT32157
CG31495-RA
CG30080-RA
CG11709-RA
CG18550-RA
CG4267-RA
CG9681-RA
CG32185-RA
CG6361-RA
CG13077-RA
CG15066-RA
CG7496-RA
CG2217-RA
CG9989-RA
Fold Change
-6.63
-3.43
-2.74
-2.53
-2.47
-2.36
2.26
2.34
2.47
2.53
2.54
2.57
2.73
2.8
2.83
2.88
2.89
2.9
2.91
3.13
3.2
3.24
3.27
3.41
3.46
3.69
3.74
3.94
3.97
3.99
4.14
4.22
4.62
4.94
5
5.36
5.66
5.8
6.97
7.22
8.94
Significant change
during media
injection?
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
Supplemental Table 6. Genes with differential expression between ets21c mutants and parental during L.
monocytogenes infection.
Gene Name
CG5139
CG14934
Cyp6a17
CG18066
wnt inhibitor of Dorsal
CG2444
CG5381
CG6675
CG14567
CG31832
CG8791
CG14516
Larval serum protein 2
CG33462
CG4409
Frost
CG33307
suppressor of white-apricot
GCR(ich)
CG18530
CG9542
CG4618
CG16704
CG11459
CG17350
CG13075
CG32922
CG18563
CG17119
lozenge
CG9416
Ady43A
CG6553
CG14205
CG13325
CG31672
CG1102
CG34037
CG1698
CG6388
CG32412
CG4733
Spn43Ad
CG11576
CG5246
yellow-f
CG17032
CG6687
spatzle
CG11919 /// CG18003
CG Number
CG5139-RA
CG14934-RA
CG10241-RA
CG18066-RA
CG8458-RA
CG2444-RA
CG5381-RA
CG6675-RA
CG14567-RA
CG31832-RA
CG8791-RA
CG14516-RB
CG6806-RA
CG30086-RA
CG4409-RA
CG9434-RA
CG16887-RA
CG3019-RA
CG5812-RA
CG18530-RA
CG9542-RA
CG4618-RA
CG16704-RA
CG11459-RA
CG17350-RA
CG13075-RA
CG32922-RB
CG18563-RA
CG17119-RA
CG1689-RA
CG9416-RA
CG1851-RA
CG6553-RA
CG14205-RA
CG13325-RA
CG31672-RA
CG1102-RA
Dm.3R.39174.0
CG1698-RA
CG6388-RA
CG32412-RA
CG4733-RA
CG1859-RA
CG11576-RA
CG5246-RA
CG18550-RA
CG17032-RA
CG6687-RA
CG6134-RA
CG30019-RB
Fold
Change
-37.73
-29.01
-26.62
-25.25
-21.75
-11.8
-11.68
-9.04
-6.79
-6.49
-6.46
-6.31
-6.3
-5.58
-5.09
-5.08
-4.85
-4.85
-4.48
-4.42
-4.36
-4.2
-4.17
-4.08
-3.91
-3.82
-3.76
-3.37
-3.33
-3.28
-3.24
-3.11
-2.95
-2.9
-2.73
-2.72
-2.71
-2.69
-2.59
-2.58
-2.58
-2.57
-2.56
-2.51
-2.49
-2.41
-2.36
-2.34
-2.32
2.37
Changed in baseline?
*
*
*
*
*
*
*
*
*
*
*
*
Supplemental Table 6. Genes with differential expression between ets21c mutants and parental during L.
monocytogenes infection (continued).
Gene Name
CG Number
Fold
Changed in
Change
baseline?
Cyp6g1
Cyp4ac1
CG6330
Adenosine deaminase-related
growth factor D
Phosphoribosylamidotransferase 2
CG3829
Insulin-related peptide
CG13833
pugilist
CG11407
CG6912
CG10361
CG15203
tolkin
Lysozyme P
CG5288
CG8854
sex-specific enzyme 2
CG34020
CG6503
CG15553
ade5
CG33047
CG8550
CG9676
CG3301
Ance-4
CG16732
CG31380
drosomycin-4
CG3597
Lipid storage droplet-1
Cyp309a1
CG4716
Open rectifier K<up>+</up>
channel 1
CG15199
Deoxyribonuclease II
Thd1
Adipokinetic Hormone receptor
CG13877
Glutactin
CG14400
CG5428
Cyp6a23
CG10550
CG31778
CG7542
CG4716
Cyp309a2
CG2081-RB
CG8453-RA
CG14032-RA
CG6330-RB
2.43
2.47
2.48
CG9621-RA
CG10078-RB
CG3829-RA
CG8167-RA
CG13833-RA
CG4067-RB
CG11407-RA
CG6912-RA
CG10361-RA
CG15203-RA
CG6863-RB
CG9116-RA
CG5288-RA
CG8854-RA
CG4979-RA
Dm.2R.20325.0
CG6503-RA
CG15553-RA
CG3989-RA
CG33047-RA
CG8550-RA
CG9676-RA
CG3301-RA
CG8196-RA
CG16732-RA
CG31380-RA
CG32282-RA
CG3597-RA
CG10374-RA
CG9964-RA
CG4716-RA
2.52
2.57
2.61
2.64
2.66
2.68
2.68
2.74
2.75
2.75
2.76
2.83
2.83
2.87
2.9
2.91
2.99
3
3.05
3.06
3.12
3.14
3.22
3.25
3.28
3.28
3.32
3.38
3.49
3.52
3.52
CG1615-RB
CG15199-RA
CG7780-RA
HDC16707
CG11325-RB
CG13877-RA
CG9280-RC
CG14400-RA
CG5428-RA
CG10242-RA
CG10550-RB
CG31778-RA
CG7542-RA
CG4716-RB
CG18559-RA
CG2081-RB
3.54
3.62
3.63
3.63
3.68
3.78
3.86
3.88
3.89
3.93
3.93
3.96
4.01
4.11
4.17
4.22
*
*
*
*
*
*
*
Supplemental Table 6. Genes with differential expression between ets21c mutants and parental during L.
monocytogenes infection (continued).
Gene Name
CG
Fold
Changed in
Number
Change
baseline?
CG6415
CG10131
white
CG31091
CG6067
Retinoid- and fatty-acid binding
protein
Cyp4ac2
rhomboid-6
CG14920
CG13607
CG3528
CG11236
CG9463
CG13313
Ugt86Dd
CG6385
CG2736
CG11598
Cytochrome b5-related
CG11659
CG12256
CG13155
CG6415-RA
CG10131-RA
CG2759-RA
CG31091-RA
CG6067-RA
4.28
4.45
4.51
4.63
4.84
CG11064-RA
CG17970-RA
CG17212-RB
CG14920-RA
CG13607-RA
CG3528-RA
CG11236-RA
CG9463-RA
CG13313-RA
CG6633-RA
CG6385-RA
CG2736-RA
CG11598-RA
CG13279-RA
CG11659-RA
CG12256-RA
CG13155-RA
5.01
5.02
5.1
5.24
5.35
5.8
6.22
6.4
6.42
6.99
7.07
8.54
8.81
9.67
20.26
30.76
76.48
*
*
*
*
*
*
*
*
*
*
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temperature stress in Drosophila melanogaster lines that are selected for recovery time from temperature
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importance for recovery from chill coma. PLoS One 5: e10925.