Supporting information 2 List of computational tools for single layer analysis Tool name Genetic constraints ACUA E-CAI MosaicFinder OperonDB RegulonDB ProOpDB DOOR FusionFinder CAIAP Genomic constraints GC-Profile GraphDNA IslandViewer MobilomeFinder PIPS PAIDB Taxonomic information CGAT MicrobesOnline Beast Microscope MEGA software Sybil VISTA EDGAR Metabolic information RAST/Model Seed MicrobesFlux FAME Pathway Tools Software COPABI CARMEN Kbase GEMSiRV RAVEN COVAIN Metabolomics MBRole MetPA Website http://www.bioinsilico.com/acua http://genomes.urv.es/CAIcal/E-CAI/ http://sourceforge.net/projects/mosaicfinder/ http://operondb.cbcb.umd.edu/cgi-bin/operondb/operons.cgi http://regulondb.ccg.unam.mx/ http://operons.ibt.unam.mx/OperonPredictor/ http://csbl1.bmb.uga.edu/OperonDB/ http://bioinformatics.childhealthresearch.org.au/software/fusionfinder/ http://www.unifi.it/scibio/bioinfo/caiap/html/ http://tubic.tju.edu.cn/GC-Profile/ http://athena.bioc.uvic.ca/virology-ca-tools/graphdna/ http://www.pathogenomics.sfu.ca/islandviewer http://db-mml.sjtu.edu.cn/MobilomeFINDER/online.htm http://www.genoma.ufpa.br/lgcm/pips/ https://www.gem.re.kr/paidb/about_paidb.php http://mbgd.genome.ad.jp/CGAT/ http://www.microbesonline.org/ http://beast.bio.ed.ac.uk/Main_Page http://www.cns.fr/agc/microscope/home/index.php http://www.megasoftware.net/ http://sybil.sourceforge.net/ http://genome.lbl.gov/vista/index.shtml http://edgar.cebitec.uni-bielefeld.de/cgi-bin/edgar_login.cgi http://rast.nmpdr.org/, http://www.theseed.org/ http://tanglab.engineering.wustl.edu/static/MicrobesFlux.html http://f-a-m-e.org/ http://bioinformatics.ai.sri.com/ptools/ http://carmen.cebitec.uni-bielefeld.de www.kbase.us http://sb.nhri.org.tw/GEMSiRV/en/GEMSiRV http://www.sysbio.se/BioMet http://www.univie.ac.at/mosys/software.html http://csbg.cnb.csic.es/mbrole/ http://metpa.metabolomics.ca/MetPA/faces/Home.jsp Reference (1) (2) (3) (4) (5) (6) (7) (8) (9) (10) (11) (12) (13) (14) (15) (16) (17) (18) (19) (20) (21) (22) (23) (24-26) (27) (28) (29, 30) (31) (32) (33) (34) (35) (36) (37) MSEA MAVEN metaP metatt Transcriptomics Bioconductor RobiNA Mayday CPTRA BASE EMMA 2 ExpressionPlot MIMAS 3.0 Epigenomics RESID db SMRT Analysis FindMod FindPept PeptideMass Proteomics DIP db STRING db Mascot MFPaQ Compid Phenomics DuctApe PhD database RetroSpect PheMaDB Single cell microscopy Tri-track RELION CellC Ecosystem information MG-RAST FANTOM MEGAN Camera IMG http://www.msea.ca. http://genomics-pubs.princeton.edu/mzroll/index.php http://metabolomics.helmholtz-muenchen.de/metap2/ http://metatt.metabolomics.ca/MetATT/ http://www.bioconductor.org/ http://mapman.gabipd.org/web/guest/robin http://www-ps.informatik.uni-tuebingen.de/mayday/wp/ http://people.tamu.edu/~syuan/cptra/ http://base.thep.lu.se/ http://www.cebitec.uni-bielefeld.de/comics/index.php/comics-software www.expressionplot.com/ http://multiomics.sourceforge.net/ http://pir.georgetown.edu/resid/ https://github.com/PacificBiosciences/SMRT-Analysis http://web.expasy.org/findmod/ http://web.expasy.org/findpept/ http://web.expasy.org/peptide_mass/ http://dip.doe-mbi.ucla.edu/dip/Main.cgi http://string-db.org/ https://www.msi.umn.edu/sw/mascot http://mfpaq.sourceforge.net/ http://users.utu.fi/lanatr/compid http://combogenomics.github.io/DuctApe/ http://apps.sbri.org/gpdb http://www.biolog.com/ http://binf.gmu.edu/wchang3/phemadb/pheno/ http://www2.mrc-lmb.cam.ac.uk/groups/scheres/software.html https://sites.google.com/site/cellcsoftware/ http://metagenomics.anl.gov/ http://www.sysbio.se/Fantom/ http://ab.inf.uni-tuebingen.de/software/megan/ http://camera.calit2.net/ http://img.jgi.doe.gov/ References 1. 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