Table S1. Summary of parentage analysis for Gobiusculus flavescens nests collected early and late in the breeding season of 2008. Nest ID, nest substrate,
mussel length and nest fullness are given for each nest collected. Total length (TL),wet body mass (WM), number of offspring in nest (# offspring), number
of offspring genotyped (# offspring assayed), number of unique reconstructed female genotypes per nest-holding male (# females) and the number of
genotyped offspring that do not match the resident nest-holding male at two or more loci (# incompatible) are listed. Nest substrate consisted of empty blue
mussels ('mussel'; Mytilis edulis), empty clams (Heterodonta; various species) and/or rock. For nests consisting of a blue mussel with two complete halves,
nest size was measured as the length of the shell (mean of the two shell halves) and nest fullness estimated in 10% increments (see Mobley et al. 2009 for
details).
Nest ID
Nest substrate
Mussel length
Nest fullness
(mm)
(%)
TL (mm)
WM (g)
# offspring
# offspring
# incompatible
# females
assayed
Early season
08-01
mussel
68
0
55.0
1.42
0
0
0
0
08-02
mussel*
---
---
44.5
0.67
759
46
0
2
08-03
mussel
66
80
49.0
0.93
4148
43
0
6
08-04
mussel
71
0
51.0
1.11
0
0
0
0
08-05
mussel
74
0
51.5
1.18
0
0
0
0
08-06
mussel
75
30
54.0
1.24
1080
48
2
2
08-07
rock
---
---
43.0
0.58
654
48
0
3
08-08
mussel
74
70
55.0
1.48
2845
45
4
7a
08-09
rock & clam
---
---
51.5
1.22
0
0
0
0
08-10b
mussel
68
5
---
---
101
39
0
2
08-11
rock & clam
---
---
47.0
0.85
0
0
0
0
08-12
mussel
68
80
56.0
1.41
3697
44
3
5
08-13
mussel*
---
---
50.5
1.09
1530
45
1
4
08-14
mussel
65
5
56.0
1.64
35
19
0
1
08-15
rock & clam
---
---
47.5
0.84
2321
47
0
4
08-16
rock & mussel
---
---
43.5
0.65
0
0
0
0
08-17
mussel
---
0
55.5
1.43
0
0
0
0
08-18
mussel
---
0
53.0
1.25
0
0
0
0
08-19
clam
---
---
51.5
1.09
3862
42
0
4
08-20c
rock
---
---
---
---
1371
48
0
2
08-21d
mussel
64
60
56.0
1.50
2327
---
---
---
08-22
mussel
51
100
54.0
1.28
2382
45
0
4
08-23
mussel
76
0
59.0
1.86
0
0
0
0
08-24
clam
---
---
55.5
1.32
1120
47
0
6
08-25
clam
---
---
51.0
1.09
0
0
0
0
08-26
mussel
52
40
50.5
1.04
971
43
9
2
08-27
mussel
53
100
53.5
1.33
3897
47
0
4
08-28b
rock
---
---
---
---
696
47
---
1
08-29
clam
---
---
50.5
1.04
2685
44
0
2
08-30
mussel*
---
---
50.0
1.08
41
22
0
1
08-32
clam
---
---
50.5
0.95
4736
47
0
6
08-33
clam
---
---
53.5
1.2
0
0
0
0
08-34
mussel
64
90
43.0
0.62
4119
44
0
5
08-35
mussel
54
60
45.5
0.67
2851
48
1
4
08-36
mussel
72
0
58.0
1.61
0
0
0
0
08-50
mussel
70
80
47.0
0.87
2533
48
0
5
08-51
mussel*
---
---
44.0
0.62
0
0
0
0
08-52d
mussel*
---
---
51.0
0.99
---
---
---
---
08-53d
mussel
74
100
48.5
0.90
---
---
---
---
Late season
08-54e
mussel
59
70
---
---
2812
48
0
4
08-55
mussel*
---
---
47.5
0.94
1771
48
0
1
08-56
mussel
75
0
44.0
0.74
0
0
0
0
44.0f
0.68
2778
47
0
5
08-57c
mussel
74
60
08-58b
mussel
75
70
---
---
7926
48
0
6
08-59
mussel
59
60
47.5
0.70
1846
47
0
3
08-60
mussel*
---
---
46.5
0.71
1398
46
0
2
08-61
mussel
68
90
46.5
0.80
6048
44
0
5
08-62
mussel
73
70
45.5
0.67
2780
45
0
6
08-63e
mussel*
---
---
42.0
0.54
2045
42
0
4
08-64d
mussel & rock
---
---
46.0
0.76
---
---
---
---
08-65
mussel
68
70
47.5
0.79
3472
48
0
5
08-66
rock
---
---
50.5
0.97
3558
48
0
3
08-67
mussel*
---
---
44.0
0.63
954
41
0
2
08-68
mussel*
---
---
40.5
0.44
1290
47
0
2
08-69
mussel*
---
---
45.5
0.72
1629
48
0
3
08-70
mussel
82
80
47.0
0.88
5518
47
0
4
08-71
mussel
78
0
44.0
0.63
0
0
0
0
08-72
mussel
69
90
45.0
0.73
5097
47
0
5
* Nest did not consist of a complete blue mussel consisting of two halves, but single shells, nests on live shells or fragments of blue mussel shells.
a
Three offspring matching the nest-holding male could not be assigned to any reconstructed female genotypes with certainty.
b
Nest-holding male not collected or collected male excluded as sire of all genotyped offspring.
c
Total length and/or wet body mass of nest-holding male was not recorded.
d
Number of offspring and/or mates could not be assessed correctly and were therefore excluded from analyses.
e
No male was collected in nest 08-54. Male genotype is consistent with the nest-holding male in 08-63 over all loci. Only 08-54 is used in calculations.
f
Measured from digital image instead of live fish.