ALFAISAL UNIVERSITY THESIS/DISSERTATION FULL TITLE by STUDENT FULL NAME A thesis/dissertation submitted in partial fulfillment of the requirements for the degree of Master of Science/Doctor of Philosophy to the Department of XXXXX of the College of XXXXX at Alfaisal University Riyadh, Kingdom of Saudi Arabia Month and Year ALFAISAL UNIVERSITY THESIS/DISSERTATION FULL TITLE by STUDENT FULL NAME Approved by: [Signature] ______________________________________________________________________ [Dr. Full Name, rank] Advisor [Department] (as listed in Alfaisal University Catalogue of current year) [Signature] ______________________________________________________________________ [Idem] Member of Committee [Signature] ______________________________________________________________________ [Idem] Member of Committee [Signature] ______________________________________________________________________ [Idem] Member of Committee Date of thesis/dissertation defense: [Day, Month, Year] ALFAISAL UNIVERSITY THESIS, DISSERTATION, PROJECT RELEASE FORM Student Name: _________________________________________________________ Last First Middle Master’s Thesis Master’s Project Doctoral Dissertation I authorize Alfaisal University to: (a) reproduce hard or electronic copies of my thesis, dissertation, or project; (b) include such copies in the archives and digital repositories of the University; and (c) make freely available such copies to third parties for research or educational purposes. I authorize Alfaisal University, three years after the date of submitting my thesis, dissertation, or project, to: (a) reproduce hard or electronic copies of it; (b) include such copies in the archives and digital repositories of the University; and (c) make freely available such copies to third parties for research or educational purposes. __________________________________________________ Signature Date This form is signed when submitting the thesis, dissertation, or project to the University Library ACKNOWLEDGMENTS Special thanks are for Mrs. ,Miss , Mr. , Dr. for their great help in the … work and for Mr. for his assistance in the technical work. My recognition and gratitude are addressed to FUNDING SOURCE (if any) its financial support and for providing . v AN ABSTRACT OF THE THESIS/DISSERTATION OF R… Ali D…. for Master of Science Major: P… Science Title: National Salmonella Surveillance in Poultry Breeders: Antimicrobial Susceptibility, Virulence, and Molecular Studies Salmonella organisms were isolated from chicken breeding farms of four provinces of Lebanon. Twelve out of 800 samples collected in the surveillance were positive for Salmonella. The biochemical characters of the 12 Salmonella isolates from chicken breeding farms were matching with the genus Salmonella. The somatic and flagellar antigens of the 12 Salmonella isolates were identified and compared to Kauffman-White-Scheme for Salmonella serotypes. Seven isolates were S. blegdam and the other five isolates were S. paratyphi B var odense. The antimicrobial resistance pattern of Salmonella isolates from chickens were compared to isolates of the same serotype from humans. Among the 9 antimicrobial agents tested in vitro for effectiveness against Salmonella isolates, only gentamicin and polymyxin B were 100% effective against chicken and human isolates. There was variation in the effectiveness of the other antimicrobial agents against Salmonella of chickens versus those of humans. S. blegdam was highly virulent in day-old chicks in comparison to S. paratyphi B var odense and the reference S. typhimurium. Using the Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis technique, the protein profiles of S. paratyphi B var odense from chickens showed a similar banding pattern. The protein profiles of S. moscow from chickens showed similarity to isolates from humans. The protein profile of S. blegdam from chickens and humans were similar. A clear difference in banding patterns was present in some S. typhimurium isolates from chickens and humans showed similarity in protein profiles. vi ILLUSTRATIONS vii TABLES viii CHAPTER I INTRODUCTION The Lebanese are also strongly anticipated at that stage among the Lebanese ISPs themselves.147 In the meantime, and in order to be able to survive within the stiff competition that is actually reigning over the Internet market in Lebanon, several Lebanese ISPs have expanded or plan to expand soon in the world; indeed, Cyberia for example has already contracted several projects in Saudi Arabia, Kuwait and Dubai,148 TerraNet plans to go to Azerbaijan, the Gulf and North Africa,149 and PSINet Lebanon is determined to operate throughout the Middle East shortly.150 A. The Internet Services Provided The products offered by the Lebanese ISPs to the public at that stage are numerous and differ slightly from the provider to the other. However, they embrace mainly in most of the cases the majority of the services mentioned hereafter.151 1. Services Targeted Mainly to the Individuals Internet - Addresses and boxes - Services Internet roaming: which allows the users to access the Internet and to check their mailboxes from anywhere in the world while traveling.152 1 2. Services Targeted Mainly to the Companies Corporate access solutions Corporate networking solutions (Intranet and Extranet solutions) 2 CHAPTER II INTERNET SERVICES ASSESSMENT The Lebanese are also strongly anticipated at that stage among the Lebanese ISPs themselves.147 In the meantime, and in order to be able to survive within the stiff competition that is actually reigning over the Internet market in Lebanon, several Lebanese ISPs have expanded or plan to expand soon in the world; indeed, Cyberia for example has already contracted several projects in Saudi Arabia, Kuwait and Dubai,148 TerraNet plans to go to Azerbaijan, the Gulf and North Africa,149 and PSINet Lebanon is determined to operate throughout the Middle East shortly.150 A. The Internet Services Provided The products offered by the Lebanese ISPs to the public at that stage are numerous and differ slightly from the provider to the other. However, they embrace mainly in most of the cases the majority of the services mentioned hereafter.151 1. Services Targeted Mainly to the Individuals Internet - Addresses and boxes: which allows the users to access the Internet and to check their mailboxes from anywhere in the world Internet roaming: which allows the users to access the Internet and to check their mailboxes from anywhere in the world while traveling.152 3 2. Services Targeted Mainly to the Companies a. Companies i. Private Companies Corporate networking ………………….. 4