Biology 13 Test Genomics and Biotechnology SB2 Name __________________________ Rev. 07-26-13 Date____________________________ Period __________________________ Matching: Use capital letters. Write the letter of the word that matches each numbered phase change in matter. (Five points each). _____ 1. technique used to manipulate an organism’s DNA by inserting the DNA of another organism A. bioinformatics _____ 2. genetic engineering technique that can make copies of specific regions of a DNA fragment B. cloning _____ 3. any of the small, circular, double-stranded DNA molecules that can be used as a vector C. DNA ligase _____ 4. organism genetically engineered by inserting a gene from another organism D. gel electrophoresis _____ 5. chemically links DNA fragments together E. gene therapy _____ 6. bacterial cells take up recombinant DNA F. genetic engineering _____ 7. area of linked genetic variations in the human genome G. genome _____ 8. newly generated DNA fragment containing exogeneous DNA H. haplotype _____ 9. creates and maintains databates of biological information, especially genomic information (http://www.bioinformatics.uga.edu/) I. plasmid _____ 10. bacterial protein that cuts DNA into fragments J. polymerase chain reaction _____ 11. uses electric current to separate certain molecules by size K. proteomics _____ 12. study of the structure and function of proteins in humans (http://www.uga.edu/mass-spec/tof-tof.htm) L. recombinant DNA _____ 13. technique to correct mutated disease-causing genes M. restriction enzyme _____ 14. process in which large numbers of recombinant DNA molecules are reproduced N. transformation _____ 15. total DNA in each cell nucleus (and/or mitochondrion) of an organism O. transgenic organism Circle the whole correct answer (not just the letter) or fill in the blank. (Five points each.) 16. We have been able to produce many kinds of dogs, from Chihuahuas to Great Danes, by using the technique of a. cloning b. genetic engineering c. natural breeding d. selective breeding 17. Plasmids are: a. X-shaped like chromosomes b. circular, single strands of RNA c. circular, double strands of DNA d. only found in eukaryotic cells 18. In 1959, the first chromosomal abnormality identified in humans was a. lupus b. Down syndrome c. Crone’s disease d. dwarfism 19. Transgenic bacteria are used to produce a. red blood cells for transfusion b. more ice crystals on crops c. insulin d. all of the above 20. Gel electrophoresis works because a. smaller DNA fragments move farther faster than larger fragments b. larger DNA fragments move farther faster than smaller fragments c. positive and negative charged fragments move together, rarely separating d. both “b” and “c” Bonus (5 points): Write the properly balanced equation for the oxidation of glucose during glycolysis.