Electronic supplementary material Parasitoid communities Species

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Electronic supplementary material
Parasitoid communities
Species of 5 parasitoid genera emerged from P. quercifex: Blastothrix sp., Metaphycus sp.
(both Hymenoptera: Encyrtidae), Coccophagus sp. (Hymenoptera: Aphelinidae), Eunotus
lividus, and Pachyneuron sp. (Hymenoptera: Pteromalidae). Individuals of Pachyneuron
sp. on sticky cards were excluded from analyses since Pachyneuron sp. can be both a
primary parasitoid (ovipositing inside scale insects) and a hyperparasitoid.
To test for differences between parasitoid communities at hot and cold sites, we
compared communities that emerged from individual P. quercifex collected on April 16,
2012 (collection methods in main manuscript). We used nonmetric multidimensional
scaling in PC-ORD 6 (McCune and Mefford 2011) to create a distance matrix of the
number of parasitoids of each genus that emerged per P. quercifex at each site. We
selected NMS to create our distance matrix, because NMS does not have distributional
assumptions. No parasitoids emerged from two of the hot sites and four of the cold sites,
so we excluded these from our analyses. To select appropriate dimensionality, we used
“Autopilot mode”. Then we used Sorensen’s (Bray-Curtis) similarity index and the
“thorough” option in PC-ORD to create our dissimilarity matrix. We ran the model for
500 iterations and began at a random configuration.
We used a multi-response permutation procedure (MRPP) in PC-ORD to test for
differences between parasitoid communities attacking P. quercifex at hot and cold sites
and found no significant difference between groups (figure S1a; A=-0.03, P=0.87). We
also provide a plot of emergence by species (figure S1b).
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