Curriculum Vitae
Akram T. Kharroubi
Place and date of birth: Jerusalem- February 17, 1954
Marital status: Married with 4 children
Address:
Al-Quds University
Department of Medical Laboratory Sciences
Tel. 0599-127800; 02-998-5371 (H)
Akram.kharroubi@gmail.com
Education:
Ph.D. in Biochemistry/Endocrinology 1983; SUNY at Buffalo, Buffalo, New
York, USA.
B.Sc. in Biochemistry, 1976; Ain- Shams University, Cairo, Egypt.
Positions Held:
Al-Quds University – Jerusalem
Faculty of Health Professions
Head, Department of Medical Laboratory Sciences
2009 – present
Department of Medical Laboratory Sciences
Associate Professor
2007 - 2009
Dean 1998
Medical Technology Department
Associate Professor
Head of the Department: 1996- 1998
College of Science and Technology- Abu Deis
Department of Biological Sciences
Assistant Professor: 1994-1995
Head of the Department: 1995-1996
George Washington University Medical Center- Washington, D.C.
Department of Physiology
Research Scientist: 1989-1994.
Principle Investigator: Dr: Joanne K. Kelleher
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Department of Biochemistry
Research Associate: 1984-1988
Al-Quds University –Jerusalem
College of Science and Technology – Abu Deis
Department of Biological Sciences
Assistant Professor and Head of the Department 1983-1984.
Ramallah Central Laboratory – Ramallah, West Bank
Director: 1977-1978.
Ramallah Hospital – Ramallah, West Bank
Medical Technologist: 1976-1977
Honors:
B.Sc. degree in Biochemistry with first class Honors - 1976
AMIDEAST/AID Scholarship for graduate study in Biochemistry – 1978
Teaching Excellence Award from AMIDEAST - 2010
Research Interest:
Use of stable isotopes to measure in vivo metabolism
1. Use of staple isotopes in measuring rates of in vivo synthesis of lipids, ketone bodies,
glucose and urea. This new technology will be applied to measure rates of synthesis
and sources of carbons for lipid and carbohydrate synthesis in humans.
Diabetes Mellitus
1. The role of Antioxidants in glucose toxicity of Diabetes Mellitus.
2. HbA1c as a diagnostic tool for Diabetes Mellitus type 2 in addition to its use to
monitor treatment of diabetic patients.
Oseoporosis
Role of parathyroid hormone (PTH) and vitamin D in osteoporosis in Palestine.
DNase:
DNase in helical bacteria
Membership In Professional Societies:
American Association for the advancement of science.
New York Academy of Science.
Sigma Chi.
Mid- Atlantic Lipid Society.
Thesis:
Hormonal Regulation of Problem Receptors in Rat Ventral Prostate, Adrenal, Kindly and
Testis; The Effect of Problem on the Dunning R3327H Rat Prostatic Adenocarcinoma.
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Publications:
1. Kharroubi, A. and slaunwhite, W.R.Jr. The Effect of Problem on the Dunning
R3327H Rat Prostatic Adenocarcinoma.
Endocrine Res. Common. 9 (3&4): 217-232 (1982-1983).
2. Kharroubi, A. and slaunwhite, W.R.Jr. Hormonal Regulation of Prpolactin (PRL)
Receptors in Male Rat Target Tissues: The Effect of Hypothyroidism and
Adrenalectomy. Endocrinology 115: 1283-1288 (1984).
3. Scallen, T.J.; Pastuszyn, A.; Noland, B.J.; Chanderbhan, R.; Kharroubi, A. and
Vahouny, G.V. Sterol Carrier and Lipid Transfer Proteins (Review).
Chem. Phys. Lipids 38: 239-261 (1985).
4. Vahouny, G.V.; Chanderbhan, R; Kharroubi, A.; Noland, B.J. and Scallen, T.J.
Sterol Carrier and Lipid Transfer Proteins (Review).
Ad. Lipid Res. 22:83- 113 (1987).
5. Kharroubi, A.; Wadsworth, J.A; Chanderbhan, R.; Weisenfeld, P.; Noland, B.;
Scallen, T.; Vahouny, G.V. and Gallo, L.L. Sterol Carrier Protein 2- Like Activity
in Rat Intestine. J.Lipid Res. 29: 287-292 (1988).
6. Chanderbhan, R.F.; Kharroubi, A.T.: Noland, B,J.; Scallen, T.J. and Vahouny,
G.V. Sterol Carrier Protein 2: Further Evidence for its Role in Adrenal
Steroidogenesis. Endocrine Res, 12: 351-370 (1986).
7. Satchithanandam, S.; Cassidy, M.M.; Kharroubi, A.T.; Calvert, R.J.; Leeds A.R.
and Vahouny, G.V. Alterations in Rat Intestinal Mucin Pattern Following
Luminal Infusion of Acetylsalicylic and Prostaglandin Derivatives. Dig. Dis.Sci.
35: 1518- 1527 (1990).
8. Lyons, H.T.; Kharroubi, A.; Wolins, N.; Tenner, S.; Chanderbhan, R.F.; Fiskum,
G. and Donaldson, R.P. Elevated Cholestrol and Decresed Sterol Carrier Protein2 in Peroxisomes from AS- 30D Hepatoma compared to Normal Rat Liver. Arch.
Biochem. Biophys, 285: 238-245 (1992).
9. Kharroubi, A.T.;Masterson, T.M. Aldaghlas, T.A.; Kennedy, K.A. and Kelleher,
J.K; Isotopomer Spectral Anylysis Estimates of Fatty Acid Synthesis in 3T3- L1
Cells. AM.J.Physiol. 263: E667-675 (1992).
10. Kelleher, J,K.; Kharroubi, A.T.; Aldaghlas, T.A,; Shambat, I.B.; Kennedy, K.A.;
Holleran, A.L. and Masterson, T.M. Isotopomer Spectral Analysis of Cholesterol
Synthesis: Applications in Human Hepatoma Cells.
AM. J.Physiol. 266: E384-E395 (1994).
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11. Satchithanandam, S.; Chanderbhan, R.; Kharroubi, A.T.; Calvert, R.J and
Klurfeld, D. Effect of Sesame Oil on Serum and Liver Lipid Profiles in the Rat.
Int J Vitam Nutr Res. 1996;66(4):386-92.
Abstracts:
12. Kharruobi, A.; Chanderbhan, R.; Fiskum, G.; Noland, B.J.; Scallen, T.J. and
Vahouny, G.V. Distribution of Sterol Carrier Proteins2 (SCP2) in Tissues and
Evidence for show Turnover in Liver and Adrenal Cortex FAASEB J. 45: 1025
(1986).
13. Kharruobi, A.; Chanderbhan, R.; Washington,J.; Noland. B.J.; Scallen , T.J.;
Diskum, G. and Vahouny, G.V. Tissue Distribution, Regulation and Physiological
Function of Sterol Carrier Protein 2. Washington Spring Symposium (1986).
14. Kharruobi, A.; Chanderbhan, R.; Noland. B.J.; Scallen , T.J.; Diskum, G. and
Vahouny, G.V. Sterol Carrier Protein 2: Aspects of Tissue Distribution and
Subcellular Levels, Regulation and Physiological Function in Liver, Adrenal and
Intestine. Second Conference on Adrenal Cortex.
Endocrine Society Annual Satellite Meeting, Anheim, CA(1986).
15. Satchithanandam, S.; Vahouny, G.V.; Kharroubi. A.; Leeds, A. and Cassidy,
M.M. The Effect of Aspirin (ASA) and Prostaglandins )PG) on Rat Intestinal
Mucin as Determined by Tracer and Antibody Methods.
FASEB J. 46: 1025 (1987).
16. Kharroubi, A.T.; Chanderbhan, R.F.; Weisenfeld, P.; Noland B.J.; Scallen , T.J.;
and Gallo, L.L. Sterol Carrier Protein 2 and Cholestrol Biosynthesis,
Esterification and Absorption by Isolated Rat Intestinal Cells
FASEB J. 2: (1988).
17. Chanderbhan, R.F.; Karroubi, A.T.; Gallo, L.L,; Noland, B.J and Scallen, T.J.
Sterol Carrier Protein2 Patricipation in ACTH- Stimulated Adrenal Steroidogensis.
FASEB J. 2: (1988).
18. Robert, L.M.; Solomon,S.; Kharroubi, A and Chanderbhan, R.
Radiommunoassay (RlA) for Lingual Lipase(LL).
American Gastroenterological Association (1988).
19. Kelleher, J.K.; Masterson, T.M. and Kharroubi, A.T. Novel Stable Isotope
Method for Quantitation Cellular Lipid and Ketone Body Synthesis.
Biophysical J.(1991).
20. Kelleher, J.K.; Masterson, T.M. and Kharroubi, A.T. New Stable Isotope
Method for Quantitation Cellular Lipid and Ketone Body Synthesis.
Diabetes 40: 248a (1991).
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21. Kharroubi, A.T.; Holleran, A.L.; Fiskum, F. and Kelleher, J.K. Effect of
Dichloroacetate on Fatty Acid Oxidarion by AS- 30D Hepatoma Cells.
FASEB J.5: A1757 (1991).
22. Kharroubi, A.T.; Masterson, T. and Kelleher, K. New 13C Gas Chromatography/
Mass Spectrometry Method for Estimating Cellular Cholesterol Synthesis. MidAtlantic Lipid Research Symposium. (1991).
23. Masterson, T.M.; Kharroubi, A.T.; Aldaghals, T.A.; Kennedy, K. and Kelleher,
J.K. Model Equation for Estimating Intracellular Dilution and Rates of Synthesis
for Stable Isotope Experiments. FASEB J. 5: A 1664 (1991).
24. Kelleher, J.K. Kharroubi, A.T.; and Ponnamperuma, R.M Stable Isotope Method
Quantitating Sources of Nitrogen for Urea Synthesis in Vitro.
FASEB J. 5: A1144 (1991).
25. Holleran, A.L.; Kharroubi, A.T.; Fiskum, G. and Kelleher, J.K. Conttibution of
Acetoacetate and Glucose to Lipid Synthesis in AS-30D Hepatoma Cells. FASEB
J.5: A756 (1991).
26. Aldaghlas, T.A.; Kharroubi, A.T.; kennedy, K.A. and Kelleher, J.K. Estimating
Triglyceride Synthesis in 3T3 Cells Using 13C Tracers and Gas Chromatography/
Mass Specttrometry. FASEB j.
27. Kharroubi A.; Masterson, T.M.; Shambat.; Kenndy, K.A. and Kelleher, J.K
Tracers and Gas Chromatography/ Mass Spectrometry. FASEB J.
28. Kharroubi, A.; Aldaghlas, T.; Holleran, A. and Kelleher, J. Mass Isotopomer
Spectral Analysis of Lipid Synthesis. Mid- Alantic Lipid Research Symposium
(1993).
29. Kelleher, K.J.; Kharroubi, A.T. and Aldaghlas, T.A. Modeling Cellular
Triglyceride Synthesis, Elongation and Recycling Using Isotopomer Spectral
Analysis (ISA) and 13C Substrates. FASEB J. (1994).
30. Kharroubi, A.T.; Aldaghlas, T.A. and Kelleher, J.K Sources of Cholesrterol
Carbons in Human Hepatoma Hep G2 Cells and Rat Cultred Hepatocytes Using
13C Tracers. Mid- Alantic Lipid Research Symposium: Lipoprotein and
Athersclerosis (1994).
31. Masterson, T.M.; Kharroubi, A.T.; Aldaghlas, T.A. and Kelleher, J.K. Probing
the Limits od Isotopomer Spectral Analysis (ISA) with the tracer Dilution
Problem .(1994).
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32. Khelleher, J.K.; Kharroubi, A.T.; Aldaghlas, T.A. Cellular Cjolesterol Synthesis,
Estimating Precursor Enrichment, Pathway Variations and De Novo Synthesis
Using Isotopomer Spectral Analysis and 13C Substrates. XIV th Washington
International Spring Symposium, 1994.
33. Kharroubi, A.T.; Aldaghlas, T.A. and Khellher, J.K.; Cellular Triglyceride
Synthesis, Elongation and Recycling: An Analysis Using Isotopomer Spectral
Analysis and 13C Substrates.
XIV th Washington International Spring Symposium, 1994.
34. A. Kharroubi, M. Akkawi, A. Ashareef, Z. Almasri, Q. Abu Remeleh, K. Bader, H. Abu
Ghoush, K. Al’hilo, M. Obeidi, U. Khammash, H. Darwish
Hemoglobin A1c (HbA1c) as a Diagnostic Tool for Diabetes Mellitus: A Step in the
Right Direction (Sixth Palestinian Conference of Lab. Medicine) March 2010.
35. M. Akkawi, A. Ashareef, Z. Almasri, Q. Abu Remeleh, K. Bader, H. Darwish, K. Al’hilo,
M. Obeidi, U. Khammash, A. Kharroubi
Total Antioxidant Status (TAS) Among Type 2 Diabetic Patients in Palestine (Sixth
Palestinian Conference of Lab. Medicine) March 2010.
36. H. Darwish, A. Abu Zahra, G. Baloshi, A. Kharroubi, K. Bader, A. Ashareef, Z.
Almasri, K. Alhilo, M. Obeidi, U. Khammash, M. Akkawi
The Gly482Ser polymorphism in the PGC-1α is not correlated with diabetes type 2
among Palestinian patients (Sixth Palestinian Conference of Lab. Medicine) March
2010.
Teaching Experience
1. Teaching the following courses; lectures and power point slides are made
available for students using e-class: Biochemistry – Endocrinology – Physiology
– Cell Biology – General Biology; as well as labs. for biochemistry, physiology,
general biology.
2. Teaching courses for Medical Technology Masters students: Advanced
biochemistry and Methods.
3. e – learning: designing and teaching Biochemistry using e learning as part of a
project applied for the first time in Palestine in 2008/2009. A biotechnology
course has also been developed in 2010.
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Research Experience
Lipid Metabolism:
1. Gas Chromatography/Mass spectrometry of lipids (cholesterol, fatty acids,
triglycerides, phospholipids, cholesterol esters and retinol esters).
2. Extraction, separation and derviatization of lipids. Thin layer chromatography and
column chromatography are employed.
3. Isolation of lipoprotein classes by ultracentrifugation including chylomicrons,
VIDL, LDL and HDL subclasses.
Tissue Culture:
1. Isolation and primary culture of rat hepatocytes.
2. Culture of cell lines like Hep G2 hepatoma cells and 3T- L1 adipocytes.
3. Isolation of different types of intestinal cells.
4. Subcellular fractionation and characterization.
Endocrine Methodologies:
1. Radiolabeling of proteins.
2. Radioimmunoassay of peptide and steroid hormones.
3. Radioreceptor assays.
4. ELISA, gel electrophoresis, ouchterlony immunodiffusion, and western blotting.
Data Analysis:
1. Computer packages like SAS and SPSS for statistical analysis.
2. Computer skills: designing courses for e class and e learning.
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