PP Site: L E V L F Q / G
TEV SIte: E N Y L F Q / G
pGEX2T-NDE1 sequence
P (the Pro is required for PP)
P (the Pro is NOT required for TEV)
TEV
(Broken
BglII)R
S
P E
N
L
Y
F
Q
G
H
M(Nde1)
BamH1
AT CCT CCA AAA TCG GAT CTG GTT CCG CGT GGA TCT CCG GAA AAC CTG TAT TTC CAG
GGC CAT ATG TCG ACT AGT GGA TCC TCG AGA ATT CAT CGT GAC TGA CTG ACG ATC
|
|
|
||| ||
CTT GAA GTC CTC TTT CAG GGA
CCC CAT ATG (to keep Nde1)
L
E
V
L
F
Q
G
P
PrescissionProtease --from pMalC2e
Here is the region around the Prescission site from pMalC2e :
AACCTCGGGGATGACGATGACAAGGTACCG CTT GAA GTC CTC TTT CAG GGA
CCC GGGGGC
Codons for the PP site are in bold. This might be useful to find the
upstream portion of your vector to make oligos for mutagenesis. The
downstream will be your gene of course unless its the blank vector.