Curriculum Vitae Personal: GH 58, Scheme 54 , Vijay Nagar, Indore 452010, India Sex : Female Nationality : Indian Family status : Single Phone : +91-731-2578478 8817108478 (Cell) Rupal Deva, Ph.D. e-mail: rupal.deva@gmail.com Work experience: From Position Place May 2012-Present Team leader, Indore, M.P, India 3.5 lakh / year Assistant Soft Vision 30,000/ professor/ College, affliated Semester Team leader to DAVV Univ, & Salary (Molecular diagnosis) at Oncquest Laboratories, Ltd,-Central Lab, Indore, M.P, India. 2011 (one semester) Symbiotech, Indore, Pithampur, M.P Senior Charite University 30,000 Reschercher School of Euro/year University Medicine, Berlin, Klinikum Germany. Charite,School of Medicine Berlin, Germany 2005-2011 Post doc at 20,000 University of Dollars/Year California, Los Angeles 2002-2005 From Position Place May 2012-Present Team leader, (Molecular Indore, M.P, India diagnosis) at Oncquest Laboratories, Ltd,-Central Lab, Indore, M.P, India. 2011 (one semester) Assistant profesor Mar 2005- Jan 2011 Senior Researcher) and Group Charite University School of leader equiv. to Asst. Prof.) Medicine, Berlin, Germany job description: Our lab is A private Medical diagnostic lab of Indore. My Present job involves diagnosis of Tuberculosis at molecular level using molecular and micro biological methods, checking the tests performed and release the patient report. as per as the Turn Around Time of the test done in our Lab for the diagnosis of tuberculosis, assisting with validation and approval of diagnosis of reports for the release to the cliniciancians and patients. Speaking to Medical professionals and informing the them regarding the report of Patients. I am also involved with assisting with grant proposal applications in our Central lab- Oncquest, Indore, M.P, India, attending clinical doctors meetings, for self-development and updating maintaining the up to date clinical practices in out NABL accredited laboratory. Scientific Vita: Education: Rerum medicarium (Ph.D.) Human medicine (Biochemistry and medical microbiology, cell biology, molecularbiology) 2002, Freie University, Berlin, Germany. Grade: Coumlaude Master of Science, (M.Sc) General Microbiology: 1997, Pune University, India. Class 1st with distinction Bachelor of Science (B.Sc (hon.): Microbiology: 1995, Pune University, India; Microbiology (honours) with zoology and chemistry as subsidiary subjects. Class 1st Fellowship/Support: 1999-2002. Received Pre-doctoral fellowship from Schering AG, Berlin, Germany and VW-Stiftung Hannover, Germany 2005-2006 : DFG, Germany Oct 2006-Dec 2006: Charite Benjamin Franklin, Germany Jan 2007-Apr 2007: DFG, Germany Mar 2008- Feb 2009- NatImmune A/S, Denmark, and Charite Mitte, Berlin Award: • • Award by Cayman Chemicals, Ann Arbor (2010) for innovative idea for development of assay kits. Awarded at 11th International Conference on Bioactive lipids in cancer, inflammation and Cancer. Poster award at Symposium on Cell Signaling and Gene Regulation (2006), Tainan. Taiwan. Poster abstract Fields of Interest: Host-pathogen-interaction, mammalian and non-mammalian eicosanoids, signal transduction, cancer biology Languages known: English, Hindi, Marathi, German and basic French at college level. Experienced in following methods: • • • Microbiology: General sterile microbiological techniques for the cultivation and handling maintenance bacteria, yeast and fungi. Light and fluorescent microscopy. Cell biology: working with cell lines and secondary culture. Handling Primary Kerationocyte culture and preparation of primary human culture of melanocytes. Molecular biology: General Molecular biological techniques including extraction of Genomic DNA and plasmid, PCR, Real-Time PCR using light cycler, and Real Time PCR gene arrays (Applied Biosystem), restriction digestion of DNA, cloning of gene in vector, Gene array analysis for differential gene expression, using clone tech. and super array real time PCR based array analysis. Molecular diagnosis of Mycobacterium tuberculosis, line and second line probe assay for drug resistance in mycobacterium (Hain). • • • • • • Basic Protein purification techniques including PAGE, Protein fractionation, sepharose column, nuclear protein preparation, Western analysis of protein Immunological Techniques: Handling of Isolation of blood cells, sub cutenous injection of mice, tissue sectioning and staining, and Proliferation assays. Techniques to study signal transduction in human cells: Western analysis of protein, RTPCR, ELISA, Real time PCR, Transfection studies in tissue culture using plasmids and dominant negatives, Immuno-staining of cells and tissue sections, TUNEL assay, and ELISA based assay for apoptosis, Immuno precipitation, CHIP assay. Mouse techniques: experienced with handling mice, giving subcutaneous injections, protein analysis and processing of tisseus for immuno-histochemical analysis. Techniques in Lipid biology: Extraction of lipids, TLC and HPLC analysis, sample preparation for GC-MS analysis. Attended Internal auditors course for laboratory quality in Indore (ISO 15829) specifies requirements for quality and competence in medical laboratories held in Indore on May1-4 2014. Management experience: As a junior group leader, I was involved in selection of candidates for projects in laboratory for their masters, Ph.D degree. Controlling and helping the management of research projects and organizing to control working of the lab instruments. directed their projects and participated actively in group meetings. I was involved in assisting of organization of conferences by our department in Berlin research lab. Involved with the lab in Indore along with different departments including microbiology, biochemistry, histopathology, collaborating towards co-operations between different heads of the department. For the swift movement of quality work in our laboratory. List of Publications: 1. Bajpai I, Shashank Monika, Gaur Ravi, Kothari Vinita and Deva Rupal. 2015 Latent TB Detection and Isolation of MDR TB Bacteria in, MP, India. Clin Microbiol 2015, 4:5 http://dx.doi.org/10.4172/2327-5073.1000223 2. Prafulla Songara, Rupal Deva*, Ila S. Bajpai, Sushma Neema4, Vinita Kothari5 (2015).Resistance Pattern of MTB Isolates from Indore region. British Journal of Medicine and Medical research. ISSN: 2231-0614,Vol.: 10, Issue.: 5, DOI : 10.9734/BJMMR/2015/18992 3. Deva Rupal, Songara, Prafulla, Neema Sushma, Kothari Vineeta, Mehra Kumar Atul and Shashank Monika. An interdisciplinary methodological approach for the diagnosis of tuberculosis in the Central state of India. (Sep. 2014), IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) e-ISSN: 2279-0853, p-ISSN: 2279-0861.Volume 13, Issue 9 Ver. VII PP 70-77 DOI: 10.13140/2.1.3152.0006 4. Prafulla Songara, Sushma Neema, Vinita Kothari, Ruchi Sachdeva, Rupal Deva 2014. Antibiotic resistance pattern in pseudomonas aeruginosa species isolated at Indore (M.P.) Journal of evolution of medical and dental sciences. Volume 3/ Issue 1. Pg-109114. DOI: 10.14260/jemds/2014/2421 5. Santosh Nigam, Roberto Ciccoli, Igor Ivanov, Marco Sczepanski, Rupal Deva. 2011. On Mechanism of Quorum Sensing in Candida albicans by 3(R)-HydroxyTetradecaenoic Acid. Current microbiology; DOI: 10.1007/s00284-010-9666-6 6. Rupal Deva, Marco Sczepanski, Sipra Saha, Maria-Patpia Zafiriou, Nadia Kerstin, PerJohan Jakobsson, Yuichi Inoue, Santosh Nigam. 2010. C. albicans activates cyclooxygenase protein, but ot it's product prostaglandin E2 in HPV16-stablized cells. European Journal of Obstetrics & Gynecology and Reproductive Biology. European Journal of Obstetrics & Gynecology and Reproductive Biology. 152(2):205-209; DOI:10.1016/j.ejogrb.2010.06.006 7. De Petrocellis L, Deva R, Manieri F, Schaefer M, Bisogno T, Ciccoli R, Ligresti A, Hill K, Nigam S, Appendino G, Di Marzo V. 2008. Biotransformation of anandamide by an unicellular fungus and chemical synthesis and pharmacological characterization and of the resulting 3-hydroxy-derivative. J Lipid Res. 50(4); DOI 10.1194/jlr.M800337-JLR200 8. Nigam S, Zafiriou MP, Deva R, Kerstin N, Geilen C, Ciccoli R, Sczepanski M, Lohse M. 2008. Hepoxilin A(3). 2007 (HXA(3)) synthase deficiency is causative of a novel ichthyosis form. FEBS Lett. 279-285; DOI: 10.1016/j.febslet.2007.12.017 9. M.P. Zafiriou, R. Deva, R. Ciccoli, A. Siafaka-Kapadai, S. Nigam. 2007. Biological role of hepoxilins: upregulation of phospholipid hydroperoxide glutathione peroxidise as a cellular response to oxidative stress? Prostaglandins Leukot Essent Fatty Acids. Prostaglandins, Leukotrienes and Essential Fatty Acids 77 (2007) 209–215; DOI:10.1016/j.plefa.2007.08.007 10. Nigam S, Zafiriou MP, Deva R, Ciccoli R, Roux-Van der Merwe R. 2007. Structure, biochemistry and biology of hepoxilins. FEBS J. 274(14):3503-12. (This article was published with the cover picture in July issue); DOI:10.1111/j.1742-4658.2007.05910.x 11. H. Neuss, X. Huang, B.K.J. Hetfeld, R. Deva, S. Nigam, J.W. Mall, W. Schwenk, W. Dubiel. 2007. COX-2 degradation is controlled by COP9 signalosome/cullin-ring ubiquitine ligase complexes and differentially influenced by coxibs. J. Mol. Med. 85(9):961-70; DOI 10.1007/s00109-007-0197-y 12. Köhler GA, Brenot A, Haas-Stapleton E, Agabian N, Deva R, Nigam S. 2006. Phospholipase A2 and Phospholipase B activities in fungi. Biochimica et Biophysica Acta (special issue on PLA2). DOI: 1761(11):1391-9; DOI:10.1016/j.bbalip.2006.09.011 13. Eghbali M, Deva R, Alioua A, Wang Y, Toro L and Stefani E. 2005. Molecular Signature of Heart Hypertrophy in Mice during Pregnancy. Circ. Res.96:1208-16. (This article was published with the cover picture in 10th June issue); DOI: 10.1161/01.RES.0000170652.71414.16. 14. Deva R, Shankaranarayanan P, Ciccoli R, Nigam S. 2003. Candida albicans induces selectively transcriptional activation of cyclooxygenase-2 in HeLa cells: pivotal roles of Toll-like receptors, p38 mitogen-activated protein kinase, and NF-kappa B. J Immunol. 171(6):3047-55; PMID:12960330 15. Deva R, Ciccoli R, Kock JLF, Nigam S. 2001. Involvement of Aspirin-Sensitive Oxylipins in Vulvovaginal Candidiasis. FEMS Microbiology Letters. 198:37-43; DOI: 10.1111/j.1574-6968.2001.tb10616.x 16. Deva R, Ciccoli R, Schewe T, Kock JLF, Nigam S. 2000. Arachidonic acid stimulates cell growth and forms a novel oxygenated metabolite in Candida albicans. Biochimica et Biophysica Acta 1486:299-311; DOI:10.1016/S1388-1981(00)00073-1 17. Book Review: Rupal Deva and Suresh I. S. Rattan. May 2009. Biogerontology. Verlag Springer Netherlands: Book review of S.L. Percival (ed): Microbiology and aging: clinical manifestations. Published online: 29 May 2009 Vol. 10(4):535 – 536. DOI: 10.1007/s10522-009-9235-1 Selected Lectures, Posters and abstracts at International conferances: 18. Rupal Deva, Roberto Ciccoli, Marco Sczepanski, Santosh Nigam (2009). 3hydroxyeicosatetraenoic acid (3-Hydroxy eicosat (3-HETE) stimulates Krüppel-like factor 13 (KLF-13) for the transcriptional activation of COX-2 promoter. 11th International Conference on Bioactive lipids in cancer, inflammation and Cancer, Cancun, Mexico P96 19. Rupal Deva, Roberto Ciccoli, Marco Sczepanski, Aynur Baharan And Santosh Nigam (2007). 3(R)-Hydroxy-tetradecaenoic acid (3R-HTDE), a metabolite of Linoleic acid, is a quorum sensing molecule in Candida albicans. 10th International Conference on Bioactive lipids in cancer, inflammation and Cancer, Montreal, Canada. P-181. 20. Rupal Deva, Maria-Patapia Zafiriou, Dominik Antoniak, Ganchimeg Ishdorj, Roberto Ciccoli, Gery Koeler, Nina Agabian, Santosh Nigam (2007). Activation of the COX-2 gene in vulvovaginal candidiasis is triggered by candidal phospholipase A2: specific role of 14-3-3 protein. 10th International Conference on Bioactive lipids in cancer, inflammation and Cancer, Montreal, Canada. P-133. 21. 3-Hydroxyoxylipins, novel fungal fatty acid metabolites, in the analysis of candidiasis: Host-pathogen interactions. Conference Paper in Prostaglandins & other lipid mediators · March 2006 22. S.Nigam, R. Deva, M.Sczepanski, S. Mergler, L. Petrocellis, V. Marzo, N Agabian (2007). 3-Hydroxy-oxylipins and derivatives, novel fungal lipid mediators produced by host pathogn interaction, activate capsaicin receptor (TRPV1): Mechanism for pain in vulvovaginnal candidiasis.Satellit Symposium on Reproductive Immunology, Aug 26-28, Rio de Janeriao, Brazil. Lecture, Presented by S.Nigam. 23. Rupal Deva, Maria-Patapia zafiriou, Roberto Ciccoli, Marco Sczepanski, Philip Wedemeier, Athanassia Siafaka-Kapadai, Santosh Nigam (2006). Deorphanization of a G protein-coupled receptor (GPCR) for hepxilin A3 as a ligand. Symposium on Cell Signaling and Gene Regulation, Tainan.Taiwan Poster abstract Pg. 30. 24. Rupal Deva, Luciano De Petrocellis, Aniello Schiano Moriello, Maria-Patapia Zafiriou, Tiziana Bisogno, Roberto Ciccoli, Vincenzo Di Marzo, Santosh Nigam(2006) Eicosanoids and transient receptor potential vanilloid type 1 (TRPV1) channels: Is 3hydroxy-eicosatetraenoic acid (3-HETE), a fungal mimetic of AA, an endovanilloid? Symposium on Cell Signaling and Gene Regulation, Tainan. Taiwan Poster abstract. Page 31. 25. Maria-Patapia Zafiriou, Rupal Deva, Roberto Ciccoli, Phillip Wedemeier, Marco Sczepanski, Athanassia Siafaka-Kapadai, Santosh Nigam (2006). Mechanistic studies on stimulation of insulin secretion by Hepoxilin A3 (HXA3) in clonal pancreatic beta cell line RINm5F. Cell Signaling and Gene Regulation, Tainan.Taiwan Poster abstract Pg. 56. 26. Rupal Deva, Ph.D.; Roberto Ciccoli, Marco Sczepanski, Aynur Baran and Santosh Nigam (2006). 3R-Hydroxy-tetradecaenoic acid (3-HTDE), a metabolite of linoleic acid, is a quorum-sensing molecule for C. albicans. 2nd Int. Conference on Non mammalian Eicosanoids, Bioactive Lipids and Plant Oxylipins, Berlin. L-34 Lecture, Presented by Rupal Deva. 27. Rupal Deva, Ph.D.; Mansoureh Eghbali, Ph.D.; Ana Ropero, Ph.D.; Ligia Toro, Ph.D.; Enrico Stefani, M.D., Ph.D. (2004). Hormonal Regulation of Heart Transcript Levels of Estrogen Receptor Alpha. American Society of Anesthesiology. A-734 Poster. 28. Ana B. Ropero, Ph.D.; Mansoureh Eghbali, Ph.D.; Rupal Deva, Ph.D.; Ligia Toro, Ph.D.; Enrico Stefani, M.D., Ph.D. (2004). A 43kDa Form of Estrogen Receptor Alpha Localizes in Several Subcellular Membrane Compartments in Adult Rat Cardiomyocytes. American Society of Anesthesiology.A-702 Poster, 29. Ana B Ropero Ph.D., Mansoureh Eghbali Ph.D., Rupal Deva Ph.D., Ligia Toro Ph.D., Enrico Stefani M.D. Ph.D. (2004). A 43kDa Form of Estrogen Receptor Alpha Localizes in Several Subcellular Membrane Compartments in Adult Rat Cardiomyocytes FASEB Research Conferences: steroid hormone receptors: Integration of plasma membraneand Nuclear signaling in hormone action. P3-2. 30. Deva R., Eghbali M., Ropero A.B., Toro L. &Stefani E. (2004). Heart Transcript Levels of Estrogen Receptor Alpha but not Beta Are Under Hormonal Control. Biophysical Journal, 255-Poster. 31. Ropero A.B., Deva R., Eghbali M., Alioua A., Toro L. &Stefani E. (2004). Estrogen Receptor Alpha Localizes In Several Subcellular Compartments In Adult Rat Cardiomyocytes. Biophysical Journal, 256-Poster. 32. Deva R., Eghbali M., Toro L. and Stefani E. (2003). Transcriptional Regulation by Estrogen of Myometrium and Cardiac Kv4.3 K+ Channels. American Society of Anesthesiology. A-1167. Poster 33. Deva, R., Shankaranarayanan, P., and Nigam, S. Candida albicans induces selectively transcriptional activation of COX-2 in HeLa cells. Pivotal roles of p38 mitogen activated kinase (p38 MAPK) and nuclear factorB).12thInternational Conference on Advances in Prostaglandin, Leukotriene and Other Bioactive Lipid Research: Basic Science and Clinical Applications, held August 25-29, 2002, in Istanbul, Turkey. 34. Ciccoli, R., Deva, R., Ivanov, I., Kock, J.L.F., and Nigam, S. Mechanistic studies towards biosynthesis of a novel hydroxyoxylipin 3,18-DiHETE in Candida albicans. 35. Deva, R. Ciccoli, R, Leo-Rossberg, I., Schewe, T, Nigam, S. Role of 3-Hydroxyoxylipins in the Morphogenesis of Candida albicans: is Aspirin as an Inhibitor of 3Hydroxyoxylipins a Therapeutic against Vulvovaginal Candidiasis? Poster (no.43) presented at 34. Wissenschaftliche Tagung der Deutschesprachigen Mykologischen Gesellschafte e.V, Myk‘ 2000, September 2000, Berlin. 36. Rupal Deva, Roberto Ciccoli, Lodewyk Kock, Tankerd Schewe and Santosh Nigam Metabolism of arachidonic acid and formation of oxylipins in fungi. Poster ( no. 18) presented at International symposium on non mammalian eicosanoids and bioactive lipids, May, 2000, Berlin Germany. 37. Santosh Nigam Rupal Deva, Roberto Ciccoli, Inka Leo-Rossberg, Dandre P. Smith, Lodewyk Kock, Tankard Schewe. Aspirin-sensitive oxylipins in Candida albicans. Implications for treatment of vulvovaginal candidiasis. Poster (no. 16) presented at International symposium on non mammalian eicosanoids and bioactive lipids, May, 2000, Berlin Germany. presented by Santosh Nigam. 38. Roberto Ciccoli, Rupal Deva, Tankard Schewe and Santosh Nigam. On the mechanism of formation of 3R-hydroxy –oxylipins in fungi. Poster (no.19) presented at International symposium on non mammalian eicosanoids and bioactive lipids, May, 2000, Berlin Germany. 39. Rupal Deva, Ph.D.; Roberto Ciccoli, Marco Sczepanski, Aynur Baran and Santosh Nigam (2006). 3R-Hydroxy-tetradecaenoic acid (3-HTDE), a metabolite of linoleic acid, is a quorum-sensing molecule for C. albicans. 2nd Int. Conference on Nonmammalian Eicosanoids, Bioactive Lipids and Plant Oxylipins, Berlin. L-34 Lecture. 40. Roberto Ciccoli, Shakti Sahi, Rupal Deva, Sandhya Singh, Maria-Patapia Zafiriou, Lodewyk Kock+, Santosh Nigam (2006) Oxygenation by COX-2 and 5-, 12- and 15lipoxygenases of 3-hydroxy-eicosatetraenoic acid, a fungal mimetic of AA, produces a cascade of novel bioactive 3-hydroxy-eicosanoids. 2nd Int. Conference on Nonmammalian Eicosanoids, Bioactive Lipids and Plant Oxylipins, Berlin. P-13 Poster. 41. Maren Lohse. Rupal Deva, Roberto Ciccoli, Santosh Nigam (2006) Serum of osteoporosis patients induces germ-tube formation in Candida albicans: involvement of receptor activator of nuclear factor-kappaB (NF-kappaB) ligand (RANKL) protein, HWP1 and Cap1 genes. 2nd Int. Conference on Nonmammalian Eicosanoids, Bioactive Lipids and Plant Oxylipins, Berlin. P-15 Poster. 42. Santosh Nigam, Ganchimeg Ishdorj, Rupal Deva, Roberto Ciccoli, Gerry Köhler, Nina Agabian (2006). Activation of the COX-2 gene in vulvovaginal candidiasis is triggered by candidal phospholipase A2: specific role of 14-3-3 protein. 2nd Int. Conference on Nonmammalian Eicosanoids, Bioactive Lipids and Plant Oxylipins, Berlin. P-17 Poster. 43. Andrew J. Norris, Rupal Deva, Miles Lopez, Julian P. Whitelegge, Arman Yaghoubian, Mai M. Brooks, Kym F. Faull (2005). Li+ Prevents Salt-Related Signal Suppression of Neutral Small Molecules Bearing a Ketone Functionality in ESI-MS. (Oral Presentation. by Andrew J. Norris). ASMS National Conference, Endogenous Analyte Quantitation, San Antonio, TX.). Oral presentation by Kym F. Faull 44. Mansoureh Eghbali, Ph.D., Rupal Deva, Ph.D., Abderrahmane Alioua, Ph.D., Ligia Toro, Ph.D., Enrico Stefani, M.D., Ph.D. Molecular and Functional Signature of Heart Hypertrophy during Pregnancy. ASA abstract A505 , 2005 45. Rupal Deva, Mansoureh Eghbali, Ana Ropero, Ligia Toro, Enrico Stefani. (2004). Hormonal Regulation of Heart Transcript Levels of Estrogen Receptor Alpha. American Society of Anesthesiology. A-734 Poster. 46. Ana B. Ropero, Mansoureh Eghbali, Rupal Deva, Ligia Toro, Enrico Stefani, (2004). A 43kDa Form of Estrogen Receptor Alpha Localizes in Several Subcellular Membrane Compartments in Adult Rat Cardiomyocytes. American Society of Anesthesiology.A-702 Poster, 47. Ana B Ropero, Mansoureh Eghbali, Rupal Deva, Ligia Toro, Enrico Stefani. (2004). A 43kDa Form of Estrogen Receptor Alpha Localizes in Several Subcellular Membrane Compartments in Adult Rat Cardiomyocytes FASEB Research Conferences: steroid hormone receptors: Integration of plasma membrane-and Nuclear signaling in hormone action. P3-2. 48. Deva R., Eghbali M., Ropero A.B., Toro L. And Stefani E. (2004). Heart Transcript Levels of Estrogen Receptor Alpha but not Beta Are Under Hormonal Control. Biophysical Journal, 255-Poster. 49. Ropero A.B., Deva R., Eghbali M., Alioua A., Toro L. and Stefani E. (2004). Estrogen Receptor Alpha Localizes In Several Subcellular Compartments In Adult Rat Cardiomyocytes. Biophysical Journal, 256-Poster. 50. Deva R., Eghbali M., Toro L. and Stefani E. (2003). Transcriptional Regulation by Estrogen of Myometrium and Cardiac Kv4.3 K+ Channels. American Society of Anesthesiology. A-1167. Poster 51. Roberto Ciccoli, Rupal Deva, Tankard Schewe and Santosh Nigam. On the mechanism of formation of 3R-hydroxy–oxylipins in fungi. Poster (no.19) presented at International symposium on non mammalian eicosanoids and bioactive lipids, May, 2000, Berlin Germany. 52. Santosh Nigam , Pierre Venter, Rupal Deva, Helmar Waiczies, M Khysar Pasha, Johan L.F. Kock, Tankerd Schewe, Dandre Smith, Studies on 3-Hydroxy oxylipins, A group of novel eicosanoids produced by fungi. Presented at the 6th International conference on Eicosanoid and other bioactive lipids in Cancer, Inflammation and Related Diseases, September 1999, Boston MA, abstract No. 67. Invited Reviewer in Journals 1. Med Microbiol Immunol, Springer 2. Biogerontology, Springer, 3. Lipids in health and diseases Biomed Central