Food and Environmental Proficiency Testing Unit
Store LENTICULE disc samples @ -20°C
Laboratory identification no. (check):Please enter your lab number Dispatch date:
28 September 2015
Final date for return of results:
30 October 2015
Results may be returned by fax, mail or e-mail
Contact details:
The Organisers - FEPTU
Public Health England
61 Colindale Avenue,
London, NW9 5EQ, UK.
Fax: +44 (0) 20 8200 8264
Tel:
+44 (0) 20 8327 7119
e-mail: foodeqa@phe.gov.uk
Shiga toxin pilot Request/Report Form
Distribution No.: STXP
Sample numbers: StxP1A, StxP1B, StxP2A and StxP2B
Download the safety data sheet:
www.gov.uk/government/publications/safety-data-sheet-for-lenticules
If you cannot examine any of these samples return your results as ‘Not examined’
Request:
I.
Determine if sample is STEC positive
Page 1 of 9
Food and Environmental Proficiency Testing Unit
Laboratory identification no. (check):
Please enter your lab number
SAMPLE: StxP1 A (including a DNA extraction process)
Store LENTICULE disc samples @ -20°C
STEC / VTEC / EHEC (Shiga / verocytotoxic / enterohemorrhagic):Not
exam
Shiga toxin
test result

Test result
CT value
If Stx positive
CT value
Identification

Stx positive

Stx 1

E.coli O157

Stx negative

Stx 2

STEC non-O157

Fail

Stx 1 & 2

eae


Fail

Fail


Other……………

Other…………
Other routine tests for similar samples:Not
exam
Additional
tests

Serogroup
(eg. O157)

O157

O26

O103

O104

O111

O145

Other…………………..

Other…………………..
Serotype
(eg. H7)
CT value
Additional comments (continue on separate sheet if necessary)
H4
Page 2 of 9
CT value
Laboratory identification no. (check):
Please enter your lab number
SAMPLE: StxP1 B (not including a DNA extraction process)
Ration of sample to master mix (eg. 5 μl sample to 25 μl of master mix)
Store LENTICULE disc samples @ -20°C
STEC / VTEC / EHEC (Shiga / verocytotoxic / enterohemorrhagic):Not
exam
Shiga toxin
test result

Test result
CT value
If Stx positive
CT value
Identification

Stx positive

Stx 1

E.coli O157

Stx negative

Stx 2

STEC non-O157

Fail

Stx 1 & 2

eae


Fail

Fail


Other……………

Other…………
Other routine tests for similar samples:Not
exam
Additional
tests

Serogroup
(eg. O157)

O157

O26

O103

O104

O111

O145

Other…………………..

Other…………………..
Serotype
(eg. H7)
CT value
Additional comments (continue on separate sheet if necessary)
H4
Page 3 of 9
CT value
Laboratory identification no. (check):
Please enter your lab number
SAMPLE: StxP2 A (including a DNA extraction process)
Store LENTICULE disc samples @ -20°C
STEC / VTEC / EHEC (Shiga / verocytotoxic / enterohemorrhagic):-
Shiga toxin
test result


Stx positive

Stx 1

E.coli O157

Stx negative

Stx 2

STEC non-O157

Fail

Stx 1 & 2

eae


Fail

Fail


Other……………

Other…………
Other routine tests for similar samples:Not
exam
Additional
tests

Serogroup
(eg. O157)

O157

O26

O103

O104

O111

O145

Other…………………..

Other…………………..
Serotype
(eg. H7)
CT value
Additional comments (continue on separate sheet if necessary)
H4
Page 4 of 9
Laboratory identification no. (check):
Please enter your lab number
SAMPLE: StxP2 B (NOT including a DNA extraction process)
Ration of sample to master mix (eg. 5 μl sample to 25 μl of master mix)
Store LENTICULE disc samples @ -20°C
STEC / VTEC / EHEC (Shiga / verocytotoxic / enterohemorrhagic):Not
exam
Test result
CT value
If Stx positive
CT value
Identification

Stx positive

Stx 1

E.coli O157

Stx negative

Stx 2

STEC non-O157

Fail

Stx 1 & 2

eae


Fail

Fail


Other……………

Other…………
Other routine tests for similar samples:Not
exam
Additional
tests

Serogroup
(eg. O157)

O157

O26

O103

O104

O111

O145

Other…………………..

Other…………………..
Serotype
(eg. H7)
CT value
Additional comments (continue on separate sheet if necessary)
H4
Page 5 of 9
CT value
Any further comments:
Authorised by:
Date reported:
Page 6 of 9
METHOD QUESTIONAIRRE (include as much detail as possible or references to full details):
Do you follow ISO13136:2012 Microbiology of food and animal feed -- Realtime polymerase chain reaction (PCR)-based method for the detection of
food-borne pathogens -- Horizontal method for the detection of Shiga toxinproducing Escherichia coli (STEC) and the determination of O157, O111, O26,
O103 and O145 serogroups
If yes please give details of
primers (eg. in house / commercial
kit)
Give details of any deviations from
ISO13136
How do you perform your
enrichment stage?
What is your DNA extraction
method?
Page 7 of 9
YES
NO
Type of molecular test
Volume tested from extraction
Details of master mix
Cycle information (eg. Tempr, times etc)
Analysis (eg. baseline threshold level
etc)
Page 8 of 9
Other (eg. Controls, etc)
Page 9 of 9