Supplementary Data S2. Climatic characteristics of the experimental sites Genetic control of yield and yield components in winter oilseed rape (Brassica napus L.) grown under nitrogen limitation. Anne-Sophie Bouchet, Nathalie Nesi, Christine Bissuel, Michel Bregeon, Amandine Lariepe, Hélène Navier, Nicolas Ribière, Mathilde Orsel, Bruno GrezesBesset, Michel Renard, Anne Laperche Author for correspondence: Nathalie Nesi, INRA, UMR 1349 IGEPP, BP35327, 35650 Le Rheu, France - nathalie.nesi@rennes.inra.fr - +33 (0)2223485707 LR11 LR12 LR13 09/14/10 to 09/16/10 09/14/11 09/05/12 73 87 97 (09/15/10 to 11/29/10) (09/14/11 to 12/10/11) (09/05/12 to 12/11/11) Key dates of rapeseed cycle Sowing date Autumn period (number of days and dates) Winter period (nb of days and dates) Flowering data (mean date and range of dates between the lines) Spring period (nb of days) Grain filling period (nb of days) Harvest 19 39 45 (11/29/10 to 12/18/10) (12/10/11 to 01/18/12) (12/12/12 to 01/25/13 ) mean=28/03/11 mean=24/03/2011 mean=21/03/2011 (03/17/11 to 04/06/11) (03/12/12 to 04/06/12) (03/08/13 to 04/17/13) 100 66 55 (12/18/10 to 03/28/11) (01/18/11 to 03/24/12 ) (01/26/12 to 03/21/13) 95 115 118 (28/03/11 to 01/07/11) (24/03/11 to 17/07/12) (22/03/11 to 17/07/12) 06/28/11 to 07/01/11 07/17/12 07/16/13 Temperature Mean daily temperature from sowing to harvest (°C) (min; max) Number of days with a mean temperature <0°C (period) 10.03 11.96 10.07 (Min= -2.4; Max.= 24.4) (Min= -3.4; Max.= 24.7) (Min= -1.7; Max.= 22.8) 8 10 5 (from 11/29/10 to 12/18/10) (from 12/10/11 to 01/18/12) (from 12/12/12 to 01/25/13) Cumulated temperatures (GDD) From sowing to harvest (°C) 2721.4 3346 3161.4 automn period 819.5 1096.2 1058.3 winter period 38.2 288.3 291.6 spring period 681.6 423 318.6 grain filling period 1182.1 1538.5 1492.9 Mean daily global radiation value (J.cm -2) 1040.59 1036.18 1061.05 Cumulated from sowing to harvest (MJ.m -2) 3007.31 3174.44 3352.91 automn period 532.81 606.32 723.6 winter period 53.07 126.42 126.41 Global radiation spring period 613.49 499.39 403.59 grain filling period 1807.94 1942.31 2099.31 17.14 -² -1 Photothermal coefficient (cal.m .°C ) Mean from sowing to harvest automn period 20.14 13.1 winter period 37.68 11.53 7.47 spring period 28.62 57.62 45.83 grain filling period 31.87 31.94 37.92 Water balance Cumulated Penman evapotranspiration From sowing to harvest (mm) 457 440.3 498.9 automn period 73.7 78.2 87.4 winter period 2.2 4.2 7.5 spring period 65 42.6 42.8 316.1 315.3 361.2 3.38 2.74 3.04 grain filling period Mean daily Penman evapotranspiration -1 (mm.day ) during the grain filling period Cumulated rainfall From sowing to harvest (mm) 455.5 566 786.5 automn period 190.5 83 272 winter period 12.5 100.5 152 spring period 148 85.5 174.5 grain filling period 104 297 188 The climatic variables were acquired during the whole crop cycle in each trial: daily air temperature (°C), rainfall (mm), Penman evapotranspiration (mm), global radiation (MJ/m²) and PAR values (MJ.m-2). Data acquisition was performed by the INRA meteorological station n°352400001 endorsed by Météo France. The four climatic periods (autumn, winter, spring and grain filling period) were defined in the Materials and Methods section.