Standard Operating Procedure
Thiophenol
Print a copy and insert into your
Laboratory Safety Manual and Chemical Hygiene Plan.
Refer to instructions for assistance.
Department:
Chemistry
Date SOP was written:
11/26/2012
Date SOP was approved by PI/lab supervisor:
Principal Investigator:
Richmond Sarpong
Internal Lab Safety Coordinator/Lab Manager:
Lab Phone:
1/13/2013
Rebecca Murphy
510-643-2485
Office Phone:
510-643-6312
Emergency Contact:
Richmond Sarpong 626-644-2407
Location(s) covered by this SOP:
Latimer 834, 836, 837, 838, 839, 842, 844, 847, 849,
and 907
(Name and Phone Number)
(Building/Room Number)
Type of SOP:
☐ Process
☒Hazardous Chemical
☐ Hazardous Class
Purpose
Thiophenol is used in acid-base reactions, alkylations, oxidation reactions, chlorinations and for
coordination to metals.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 108-98-5
Class: Flammable liquid
Molecular Formula: C6H6S
Form (physical state): liquid
Color: clear
Boiling point: 168.3°C
Thiophenol
UCLA- EH&S
1
Date: 1/8/2013
CW/SH:
Potential Hazards/Toxicity
Thiophenol is a flammable liquid and hazardous in case of skin contact.
Harmful if swallowed, inhaled, or in contact with the skin or eyes. Avoid breathing spray/mist.
May cause respiratory tract irritation.
Severe overexposure can result in death. May be fatal if absorbed through the skin and have systemic
effects on respiration and behavior/central nervous system.
Has the following exposure limits: TWA: 0.5 from ACGIH (TLV) [United States]
Has the following toxicity data:
ORAL (LD50): Acute: 46.2 mg/kg [Rat], 267 mg/kg [Mouse].
DERMAL (LD50): Acute: 134 mg/kg [Rabbit].
Engineering Controls
Handle using a chemical fume hood with good ventilation and electrically grounded lines and equipment.
Personal Protective Equipment (PPE)
NOTE: Lab-specific information on PPE selection may be included in Lab Specific Instructions.
Respiratory protection
NOTE: Lab personnel intending to use/wear a respirator mask must be trained and fit-tested by
EH&S. This is a regulatory requirement.
Refer to 8 CCR 5144 for selection of respirators. A respiratory protection program that meets 8
CCR 5144 must be followed whenever workplace conditions warrant use of a respirator.
Respirators should be used only under any of the following circumstances:






As a last line of defense (i.e., after engineering and administrative controls have been
exhausted).
When Permissible Exposure Limit (PEL) has exceeded or when there is a possibility that
PEL will be exceeded.
Regulations require the use of a respirator.
An employer requires the use of a respirator.
There is potential for harmful exposure due to an atmospheric contaminant (in the
absence of PEL)
As PPE in the event of a chemical spill clean-up process
Thiophenol
UCLA- EH&S
2
Date: 1/8/2013
CW/SH:
Hand Protection
Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique
(without touching glove's outer surface) to avoid skin contact with this product. Dispose of
contaminated gloves after use in accordance with applicable laws and good laboratory
practices. Wash and dry hands.
For glove selection, go to: http://ehs.berkeley.edu/hs/63-laboratory-safety/94-glove-selectionand-usage.html
Eye Protection
Safety glasses with side shields or tightly fitting safety goggles. Use face shield (8-inch
minimum) when appropriate. Use equipment for eye protection tested and approved under
appropriate government standards such as NIOSH (US) or EN 166(EU) or ANSI Z87.1.
Skin and Body Protection
Long pants, closed-toed and closed-heeled shoes, cotton-based clothing/attire, and flame
resistant (where appropriate) lab coat must be worn for protecting against chemical hazards.
Additional PPE may be required if procedures or processes present additional risk. It is the
responsibility of the PI to ensure that any additional PPE requirements are identified and
communicated to research staff.
Storage
Thiophenol should be stored in a well-ventilated area away from incompatible substances. Use
unbreakable secondary containment for the storage of acutely toxic chemicals. If the materials
are volatile (or could react with moisture or air to form volatile toxic compounds), containers
should be in a ventilated storage area. All containers of acutely toxic chemicals should be
clearly labeled with chemical composition, known hazards, and warnings for handling.
Chemicals that can combine to make toxic materials (e.g., acids and inorganic cyanides, which
can generate hydrogen cyanide) should not be stored in the same secondary containment.
Chemicals that have a limited shelf life need to be tracked and monitored. Chemicals that
require refrigeration should be stored appropriately.
Spill Response and Exposure Response
Before beginning work with chemicals, review the relevant SOPs, Safety Data Sheets, and
other chemical safety resources. Develop specific procedures for emergency response and
chemical exposure or injury to staff, including any special first aid measures required for the
relevant chemicals.
Spill and Accident Procedure
Note: These responses may not apply to all acutely toxic chemicals. Please review specific
chemical SOPs and SDSs for specific response to spills and accidents. Always dial 911 and
510-642-9090 for assistance.
Thiophenol
UCLA- EH&S
3
Date: 1/8/2013
CW/SH:
Chemical Spill Dial 911
Spill – Assess the extent of danger. Help contaminated or injured persons. Evacuate the spill
area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or
absorbent material. Keep others from entering contaminated area (e.g., use caution tape,
barriers, etc.). Dial 911 and 510-642-9090 for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in
emergency shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S
immediately. Follow up with a call to 510-642-9090 to report the incident.
Chemical Splash Into Eyes – Immediately rinse eyeballs and inner surfaces of eyelids with
water from the emergency eyewash station for 15 minutes by forcibly holding the eye open.
Seek medical attention. Notify supervisor and EH&S immediately. Follow up with a call to 510642-9090 to report the incident.
Medical Emergency Dial 911
Life Threatening Emergency, After Hours, Weekends and Holidays – Dial 911 or go to the
nearest emergency room. Note: All serious injuries must be reported to EH&S within 8 hours.
Follow up with a call to 510-642-9090 to report the incident.
Non-Life Threatening Emergency – Go to the Occupational Health Facility (Tang Health
Center). After hours go to the nearest emergency room. Note: All serious injuries must be
reported to EH&S within 8 hours. Follow up with a call to 510-642-9090 to report the incident.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the
affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Go to the
Occupational Health Facility (Tang Health Center). After hours go to the nearest emergency
room. Note: All needle stick/puncture exposures must be reported to EH&S within 8 hours.
Follow up with a call to 510-642-9090 to report the incident.
Hazardous Waste Disposal
General hazardous waste disposal guidelines:
Label Waste
Label all containers with the label provided at http://ehs.berkeley.edu/hm/279-new-hazardous-wasteprogram-hwp.html. See the EH&S Fact Sheet, “Hazardous Waste Management” for general
instructions on procedures for disposing of hazardous waste.
Dispose of Waste


Dispose of regularly generated chemical waste within six (6) months.
Call EH&S for questions
Safety Data Sheet (SDS) Location
SDS can be accessed online at http://ucmsds.com
Thiophenol
UCLA- EH&S
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Date: 1/8/2013
CW/SH:
Protocol/Procedure (Add lab specific Protocol/Procedure here)
--All work with thiophenol should be performed in a fume hood with the proper PPE worn at all times.
-
Protocol/Procedure for– Thiophenol
CAS #(s) 108-98-5
Preparation
Know the location of the nearest spill kit, fire extinguisher, eyewash, and safety shower before beginning work.
Eliminate any incompatible materials from the potential spill area.
Never work alone. Make sure there is another worker present who is also trained in the thiophenol SOP
Lab-specific
Information
All work for this procedure is to take place in the designated fume hood.)
Procedure/Use
Scale
Thiophenol is used
in acid-base
reactions,
alkylations,
oxidation reactions,
chlorinations and
for coordination to
metals.
5 mL
Engineering
Controls/Equipment
Up to 10 ml maximum used
at one time.
PPE (eye, face, gloves,
clothing)
Eye protection: Safety glasses
with side shields or tightly fitting
safety goggles. Use face shield
(8-inch minimum) when
appropriate.
Face protection:
Gloves: For light incidental (e.g.,
splash) use 0.3mm (12 mil) Viton
butyl rubber gloves or for heavy
extended (e.g. immersion),
recommend 0.7mm (28 mil)
fluorinated rubber gloves such as
Sigma Aldrich Vitojet. Gloves.
Clothing: Long pants, closedtoed and closed-heeled shoes,
Thiophenol
UCLA- EH&S
5
Date: 1/8/2013
CW/SH:
Procedure Steps and Special
Precautions for this Procedure
After adding the desired amount of
thiophenol, all needles, syringes,
gloves, and flasks should be washed in
a dilute aqueous solution of bleach for 1
hour, due to its strong stench.
cotton-based clothing/attire, and
flame resistant (where
appropriate) lab coat must be
worn for protecting against
chemical hazards.
Notes
Initials of
individuals using
this procedure
Thiophenol
UCLA- EH&S
Any deviation from this SOP requires approval from PI.
6
Date: 1/8/2013
CW/SH:
Documentation of Training (signature and initials of all users is required)

Prior to conducting any work with Thiopenol, designated personnel must provide training to his/her
laboratory personnel specific to the hazards involved in working with the specific chemical(s) used,
work area decontamination, and emergency procedures.

The Principal Investigator must provide his/her laboratory personnel with a copy of this SOP and a
copy of the SDS provided by the manufacturer.
I have read and understand the content of this SOP:
Name
Signature
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Thiophenol
UCLA- EH&S
Date
7
Date: 1/8/2013
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Thiophenol
UCLA- EH&S
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Date: 1/8/2013
CW/SH: