Supplementary Tables 2. Primers designed for the different experiments perfomed
during the the work.
Supplementary Table 2.1.
Oligonucleotides used for obtaining promoter
amplicon used for cloning and primers needed for direct mutagenesis. KAPA: KAPA
Taq DNA Polymerase (KAPA Biosystems, Massachusetts, USA)
Sequence (5'--> 3')
Luci_F
Luci_R
Mut_F
Mut_R
CCGGTACCTTCAGAACCAGCCGAATAGACC
(KPN1)
CCCTCGAGAGTGACATCAACTGGGCAATTT (Xho1)
GAGAGTTTACTTTTCTGGACTTCCCAGAGCTG
TGCCAGCAGCTCTGGGAAGTCCAGAAAAGTA
Tm
Amplicon
size
55ºC
1527pb
PCR kit
KAPA
1
Supplementary Table 2.2. Primers designed for bisulphite conversion PCR and
sequencing. One Taq Hot Start DNA Polymerase (New England Biolabs, Ipswich, MA,
USA); Amplitaq Gold 360 DNA Polymerase (Applied Biosystems, CA, USA); EPIK
Amplification kit
Primers (Top strand)
Amplicon
sizes
Tm(ºC) PCR kit
F5’GGGAGAATTTGGGATTGTGT3’
770pb
58
R5’CATTCCCAACCCTACCTACC3’
EPIK
Amplification kit
F5’AGGTTTTGGTAGGTATTTGTTG3’
R5’CCATTCCCAACCCTACCT3’
499pb
56
One Taq Hot
Start DNA
Polymerase
56
Amplitaq Gold
360 DNA
Polymerase
56
Amplitaq Gold
360 DNA
Polymerase
56
Amplitaq Gold
360 DNA
Polymerase
62
Amplitaq Gold
360 DNA
Polymerase
63
Amplitaq Gold
360 DNA
Polymerase
Rme5’CCGTTCCCAACCCTACCT3’
F5’AGGTAGGGTTGGGAATGG3’
R5’CCCCTCACCTTAACTAAAAAA3’
363pb
F5’AGTTTTTTAGTTAAGGTGAGGG3’
R5’TAACAAAAAATAACCTCATTCC3’
373pb
F5’GGAATGAGGTTATTTTTTGTTAGT3’
R5’AATTTAAAATCAACTCCCTCAA3’
274pb
F5’GGAGGAGGAGGAGGTGGAGA3’
R5’CTATCACCCTCCAAACAAACAAA3’
One Taq Hot
Start DNA
Polymerase
691pb
F5’GGGAGTTTTTTGTAGGAGGG3’
705pb
2
R5’ACAACACTTACACCAATACCACC3’
Reverse complementary
Amplicon
sizes
EPIK
Amplification kit
Tm(ºC) PCR kit
F5’GTGTTAGTAGTTTTGGGAAGTT3’
R5’CTAATCACCCCTCGCTCA3’
418pb
57
Amplitaq Gold
360 DNA
Polymerase
57
Amplitaq Gold
360 DNA
Polymerase
F5’TTTTTATTTTGGTTGAAGGATT3’
R5’AACTTCCCAAAACTACTAACAC3’
511pb
3