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Table S1. Primers used in plasmid construction and other experiments.
Primer
NbCaM-cds-F
NbCaM-cds-R
NbCaM-F
NbCaM-R
NtCaM-F
NtCaM-R
SlCaM-F
SlCaM-R
TRV-NbCaM-F
TRV-NbCaM-R
TRV-NtCaM-F
TRV-NtCaM-R
TRV-SlCaM-F
TRV-SlCaM-R
A-CaM-F
A-CaM-intron-R
B- CaM-intron-F
B-Intron-R
C-CaM-F
C-CaM-R
PVX-CaM-F
PVX-CaM-R
S-FP-F
S-FP-R
Y10A-F
Y10A-R
Y35DNA1-F
Y35DNA1-R
35S-F
35S-R
CaM-p-F
CaM-p-R
βC1-p-F
βC1-p-R
GFP-p-F
GFP-p-R
PVX-CP-F
PVX-CP-R
U6
G1
G2
Sequence
5'-TTACTAGTTTAGTTGTTATACG-3'
5'-CCAAGGCCAAAGAATTATGTACATG-3'
5'-GGATCCATGTGCATGGAATCA-3'
5'-CTGCAGTTAACTTGTCATCATAG-3'
5'-CGGGGTACCATGGAATCAGTTTCTGTACC-3'
5'-AACTGCAGTTAACTTGTCATCATAGCTTTG-3'
5'-CGGGGTACCATGTCTAATATGGTGTTAGTTAC-3'
5'-AACTGCAGCTAACTTGTCATCATAACTTTG-3'
5'-CGGAATTCTCTAGAAATCAGTTTCTGTACCTAGTGTTG3'
5'-CCGCTCGAGCATCGCCGTCGGAATCCGATAGTC-3'
5'-CGGAATTCTCTAGATTTCTGTACCTAGTGTTGAAAAC-3'
5'-CCGCTCGAGCATCCCCGTCGGAATCCGATAGCC-3'
5'-CGGAATTCTCTAGATGGTGTTAGTTACTGTTTCCATGG-3'
5'-CCGCTCGAGCCATCTCCGCCTCCTCCACCG-3'
5'-GAGCTCTGTGCATGGAATCAGTTTCTGTACC-3'
5'-ATCAGACTTACAACGTATACATTCCAAATGCT-3'
5'-AGCATTTGGAATGTATACGTTGTAAGTCTGAT-3'
5'-GGATCCGCTCTATCTGCTGGGTCCAAATC-3'
5'-GGATCCATACATTCCAAATGCTCCTATC-3'
5'-CTGCAGTGTGCATGGAATCAGTTTCTG-3'
5'-ATCGATATGTGCATGGAATCAAGTTTC-3'
5'-GTCGACTTAACTTGTCATCATAGCTTTG-3'
5'-GAGCTCCAGTGGAGAGGGTGAAGGTGATGC-3'
5'-GGATCCCTTTGATTCCATTCTTTTGTTTG-3'
5'-ATGTGGGATCCTCTGCTCAACGAGTTTC-3'
5'-CATCCTCAGACCTTGCGTTTCTTAAGAG-3'
5'-CCATCGATATGCCTTCAATTACCTCTGTC-3'
5'-GGTGTCGACTTAGTCCAAATACTCGTCGC-3'
5'-ACATGGTGGAGCACGACACG-3'
5'-GAGGAAGGGTCTTGCGAAGG-3'
5'-GGATCCTAATACGACTCACTATAGGGAGAAATCCTCCAAACCCAACA-3'
5'-TTCACTCAAAAAGGCAACGACAACAACAACTATTAC-3'
5'-GGATCCTAATACGACTCACTATAGGGAGAACATCATACTCATCCCCT-3'
5'-AGTCACCAGCATTAGCCAAGAAGAAATTCATG-3'
5'-CATGAGTAAAGGAGAAGAACTTTTC-3'
5'-TTCATATGATCTGGGTATCTTG-3'
5'-CACAACACAGCCCATAGGGTC-3'
5'-TTAACATCCAGTTCCATACCACTG-3'
5'-TTGCGTGTCATCCTTGCGCAGG-3
5'-GGATCCAAGGAGATATAACAATGAAGACTAATCTTTTTCT-3
5'-CTTTCTCATCTTTTCACTTCTCCTATCATTATCCTCGGCC-3
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Purpose
To amplify the complete coding region of Nbrgs-CaM
To amplify the complete coding region of Nbrgs-CaM
pCHF3-35S-NbCaM, for transgenic over-expression and transient agro-infiltration
pCHF3-35S-NbCaM, for transgenic over-expression and transient agro-infiltration
pCHF3-35S-NtCaM, for transient agro-infiltration
pCHF3-35S-NtCaM, for transient agro-infiltration
pCHF3-35S-SlCaM, for transient agro-infiltration
pCHF3-35S-SlCaM, for transient agro-infiltration
pTRV-NbCaM, for VIGS of Nbrgs-CaM via TRV vector
pTRV-NbCaM, for VIGS of Nbrgs-CaM via TRV vector
pTRV-NtCaM, for VIGS of Nbrgs-CaM via TRV vector
pTRV-NtCaM, for VIGS of Nbrgs-CaM via TRV vector
pTRV-SlCaM, for VIGS of Nbrgs-CaM via TRV vector
pTRV-SlCaM, for VIGS of Nbrgs-CaM via TRV vector
pCHF3-35S-dsCaM, for down-regulation of Nbrgs-CaM
pCHF3-35S-dsCaM, for transgenic down-regulation of Nbrgs-CaM
pCHF3-35S-dsCaM, for transgenic down-regulation of Nbrgs-CaM
pCHF3-35S-dsCaM, for transgenic down-regulation of Nbrgs-CaM
pCHF3-35S-dsCaM, for transgenic down-regulation of Nbrgs-CaM
pCHF3-35S-dsCaM, for transgenic down-regulation of Nbrgs-CaM
pGR-PVX-CaM, for over-expression of Nbrgs-CaM via PVX vector
pGR-PVX-CaM, for over-expression of Nbrgs-CaM via PVX vector
pCHF3-FP, for inducing GFP silencing
pCHF3-FP, for inducing GFP silencing
To make probe for the detection of TYLCCNV in Southern blot
To make probe for the detection of TYLCCNV in Southern blot
To make probe for the detection of DNA1 in Southern blot
To make probe for the detection of DNA1 in Southern blot
PCR screening of transgenic plants containing CaMV 35S promoter
PCR screening of transgenic plants containing CaMV 35S promoter
To make probe for the detection of Nbrgs-CaM in Northern blot
To make probe for the detection of Nbrgs-CaM in Northern blot
To make probe for the detection of βC1 in Northern blot
To make probe for the detection of βC1 in Northern blot
To make probe for the detection of GFP in Northern blot
To make probe for the detection of βC1 in Northern blot
To make probe for the detection of PVX CP in Northern blot
To make probe for the detection of PVX CP in Northern blot
DNA oligo probe for the detection of U6 in siRNAs blot
DNA oligo probe for the detection of G siRNAs
DNA oligo probe for the detection of G siRNAs
G3
F1
F2
F3
P1
P2
P3
Y10A-1-siRNA
Y10A-2-siRNA
Y10A-3-siRNA
Beta-1-siRNA
Beta-2-siRNA
Beta-3-siRNA
NbSu-1-siRNA
NbSu-2-siRNA
NbSu-3-siRNA
GAPDH-q-F
GAPDH-q-R
NtCaM-q-F
NtCaM-q-R
SlCaM-q-F
SlCaM-q-R
NbAGO1-q-F
NbAGO1-q-R
NbAGO4-q-F
NbAGO4-q-R
NbDCL1-q-F
NbDCL1-q-R
NbDCL2-q-F
NbDCL2-q-R
NbDCL3-q-F
NbDCL3-q-R
NbDCL4-q-F
NbDCL4-q-R
NbSGS3-q-F
NbSGS3-q-R
NbRDR1-q-F
NbRDR1-q-R
NbRDR2-q-F
NbRDR2-q-R
NbRDR6-q-F
NbRDR6-q-R
NbSu-q-F
NbSu-q-R
5'-GAATTCAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTC-3
5'-AATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATA-3
5'-CGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAA-3
5'-CTACCTGTTCCATGGCCAACACTTGTCACTACTTTCTCTT-3
5'-CAAGTTGGAATACAACTACAACTCCCACAACGTATACATC-3
5'-ATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCA-3
5'-AAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGC-3
5'-CCCCTATCACCCATTATCATCAC-3'
5'-TTGCAGTTAGTTGAAAAAACCTACTCTCCTG-3'
5'-CCCGCAGATATAGTCATTTCCACTCCCGCCT-3'
5'-TCATATATTTGAATACGTATATATATGTATT-3'
5'-GAATTTGTAAATACATCATACTCATCCCCTA-3'
5'-GGTGGGATAATCCCATGGTGTCTGTATGGGA-3'
5'-CAGGGCAGAGTCAAGGGAGGAAGTTTTATGGAGGGATTAG-3'
5'-AAGTCAGCCCTGCTCAAGAACAGGGTCAGAAACTTGCTGA-3'
5'-ATGTCTTTTGCTGAATGTAATTGATCCAAAGATTGGAGGT-3'
5'-GCAGTGAACGACCCATTTATCTC-3'
5'-AACCTTCTTGGCACCACCCT-3'
5'-ATGGAAAAGTGTCACCGGCTGAGC-3'
5'-TTCCAAATGCTCCTATCAATTCACTC-3'
5'-GGGTATTTACGTACTTTGATGAGAATGG-3'
5'-TATTCCTCTCTTCCTCCATTCCTTCC-3'
5'-CTGGCGTGGCTTCTATCAAAGTATT-3'
5'-CACCTTTACACCTCTCAGTGCCTTC-3'
5'-GGAACTATGACTTCTACCTGTGTGCC-3'
5'-AACTTCATCCATTGTCCAACTTGTGT-3'
5'-TCTTTACATACACAGATCTCCCC-3'
5'-ACCTGGTTTTGATAGTTCATTTT-3'
5'-GAAGGCAGAAGACTAAAAGTAAGAGG-3'
5'- CTACAAGCAGAGAAGGATCATGGAAC-3'
5'-GAGTCAACAGAGCGTAAATCCAAGTC-3'
5'-CTGTCGTTCTAGCTCATACAGCATGA-3'
5'- GCAAAGACTGAAGTTAGGTGTAGCAA-3'
5'-TTTCATCAGCAAGTGGAATAAAGATT-3'
5'-GTTCCTCCTGCTCTGAAGAATGG-3'
5'-GGGCAGTGCCACTGTCGTTCAGG-3'
5'-GACAACTTCCAAAATCTCCATCCA-3'
5'-TTCTCCCACTCCTCATCAACAAAA-3'
5'-GGCACCAACTCATTCTAACCTC-3'
5'-CCAACCTATAACACTCCCCCTT-3'
5'-GTATGCGGAACTTGAATAGGAATGTG-3'
5'-GGTCTTCTGCAAAAAACCAGGCAGA-3'
5'-GCTTCTACACCCTTGTCTTCTCG-3'
5'-CCCCTATCACCCATTATCATCAC-3'
DNA oligo probe for the detection of G siRNAs
DNA oligo probe for the detection of F siRNAs
DNA oligo probe for the detection of F siRNAs
DNA oligo probe for the detection of F siRNAs
DNA oligo probe for the detection of P siRNAs
DNA oligo probe for the detection of P siRNAs
DNA oligo probe for the detection of P siRNAs
DNA oligo probe for the detection of TYLCCNV siRNAs
DNA oligo probe for the detection of TYLCCNV siRNAs
DNA oligo probe for the detection of TYLCCNV siRNAs
DNA oligo probe for the detection of TYLCCNB siRNAs
DNA oligo probe for the detection of TYLCCNB siRNAs
DNA oligo probe for the detection of TYLCCNB siRNAs
DNA oligo probe for the detection of NbSu siRNAs
DNA oligo probe for the detection of NbSu siRNAs
DNA oligo probe for the detection of NbSu siRNAs
Relative qRT-PCR analysis of NbGADPH
Relative qRT-PCR analysis of NbGADPH
Relative qRT-PCR analysis of NtCaM
Relative qRT-PCR analysis of NtCaM
Relative qRT-PCR analysis of SlCaM
Relative qRT-PCR analysis of SlCaM
Relative qRT-PCR analysis of NbAGO1-1
Relative qRT-PCR analysis of NbAGO1-1
Relative qRT-PCR analysis of NbAGO4-1
Relative qRT-PCR analysis of NbAGO4-1
Relative qRT-PCR analysis of NbDCL1
Relative qRT-PCR analysis of NbDCL1
Relative qRT-PCR analysis of NbDCL2
Relative qRT-PCR analysis of NbDCL2
Relative qRT-PCR analysis of NbDCL3
Relative qRT-PCR analysis of NbDCL3
Relative qRT-PCR analysis of NbDCL4
Relative qRT-PCR analysis of NbDCL4
Relative qRT-PCR analysis of NbSGS3
Relative qRT-PCR analysis of NbSGS3
Relative qRT-PCR analysis of NbRDR1
Relative qRT-PCR analysis of NbRDR1
Relative qRT-PCR analysis of NbRDR2
Relative qRT-PCR analysis of NbRDR2
Relative qRT-PCR analysis of NbRDR6
Relative qRT-PCR analysis of NbRDR6
Relative qRT-PCR analysis of Nb-Su
Relative qRT-PCR analysis of Nb-Su
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