1
Online supplementary table:
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3
Table S1: Origin of yeast genotypes
4
Detailed description (if available) of the origin, year of isolation, source of isolation and
5
reference, as well as the mean attraction (±SE) to Drosophila simulans (see Palanca et al.
6
2013), for each of the Saccharomyces cerevisiae genotypes tested. These total 97 distinct yeast
7
genotypes: five yeasts isolated from New Zealand caught D. simulans, 51 different S. cerevisiae
8
genotypes spanning the genetic and ecological diversity of the natural S. cerevisiae population
9
characterised in New Zealand (Goddard et al. 2010; Gayevskiy & Goddard 2012), 36 S.
10
cerevisiae genotypes spanning the genetic and ecological diversity globally described in Liti et
11
al.(2009) and five genotypes used in commercial wine making (Richards et al. 2009).
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Yeast genotype
Origin, Year of isolation, Source, Reference
mean AI (±SE)
CDRDR_sf_H2
New Zealand, 2011, Fermenting grape juice, Goddard collection (unpublished)
-0.0771 (± 0.2330)
CRIRIwf_A11
New Zealand, 2011, Fermenting grape juice, Goddard collection (unpublished)
0.1067 (± 0.2696)
Fly_KR_168.1
New Zealand, 2012, Drosophila simulans, Goddard collection (unpublished)
0.0452 (± 0.1445)
Fly_KR_35.1
New Zealand, 2012, Drosophila simulans, Goddard collection (unpublished)
-0.3037 (± 0.0245)
Fly_KR_78.3
New Zealand, 2012, Drosophila simulans, Goddard collection (unpublished)
0.2193 (± 0.0784)
Fly_WI_300.4
New Zealand, 2012, Drosophila simulans, Goddard collection (unpublished)
0.0591 (± 0.1094)
Fly_WI_300.5
New Zealand, 2012, Drosophila simulans, Goddard collection (unpublished)
-0.0568 (± 0.3423)
HB_C_KOROKIPO_12
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
0.2146 (± 0.2072)
HB_C_KOROKIPO_3
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
-0.0677 (± 0.1662)
HB_C_OMARUNUI_14
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
0.0638 (± 0.2831)
HB_C_OMARUNUI_6
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
0.3414 (± 0.0740)
HB_C_OMARUNUI_7
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
-0.2195 (± 0.1938)
HB_C_TUKITUKI1_16
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
0.1732 (± 0.2562)
HB_C_TUKITUKI2_10
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
0.2431 (± 0.1959)
HB_S_BILANCHER_12
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
-0.2000 (± 0.1607)
HB_S_BILANCHER_6
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
0.0715 (± 0.1199)
HB_S_GIMBLETTROAD_14
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
0.1022 (± 0.0511)
HB_S_GIMBLETTROAD_16
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
-0.0165 (± 0.5549)
HB_S_GIMBLETTROAD_22
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
-0.0492 (± 0.1556)
HB_S_GIMBLETTROAD_5
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
0.0393 (± 0.1193)
HCNKIsf_G7
New Zealand, 2011, Fermenting grape juice, Goddard collection (unpublished)
0.1045 (± 0.1528)
HCNTHsf_C5
New Zealand, 2011, Fermenting grape juice, Goddard collection (unpublished)
0.2308 (± 0.2166)
HCNTHsf_F8
New Zealand, 2011, Fermenting grape juice, Goddard collection (unpublished)
0.2591 (± 0.0364)
HPRMAwf_D10
New Zealand, 2011, Fermenting grape juice, Goddard collection (unpublished)
0.0667 (± 0.0333)
HPRMTsf_H7
New Zealand, 2011, Fermenting grape juice, Goddard collection (unpublished)
0.2216 (± 0.1443)
MARARsf_A10
New Zealand, 2011, Fermenting grape juice, Goddard collection (unpublished)
-0.3069 (± 0.1985)
MTKSKsf_E2
New Zealand, 2011, Fermenting grape juice, Goddard collection (unpublished)
0.4144 (± 0.1786)
NSEBRsf_A9
New Zealand, 2011, Fermenting grape juice, Goddard collection (unpublished)
0.0855 (± 0.1804)
NSERVsf_F8
New Zealand, 2011, Fermenting grape juice, Goddard collection (unpublished)
0.3573 (± 0.2041)
Sol7-2
New Zealand, 2005, Vineyard soil, Goddard et al. 2010
0.0550 (± 0.0784)
1
T.52_2H
New Zealand, 2010, Fermenting grape juice, Goddard collection (unpublished)
0.0119 (± 0.0676)
T.52_3A
New Zealand, 2010, Fermenting grape juice, Goddard collection (unpublished)
-0.0508 (± 0.1723)
T.52_3C
New Zealand, 2010, Fermenting grape juice, Goddard collection (unpublished)
0.0965 (± 0.1028)
T.52_5A
New Zealand, 2010, Fermenting grape juice, Goddard collection (unpublished)
-0.2208 (± 0.1329)
T.52_5E
New Zealand, 2010, Fermenting grape juice, Goddard collection (unpublished)
0.0006 (± 0.2977)
T16
New Zealand, 2005, Fermenting grape juice, Goddard et al. 2010
0.0360 (± 0.1107)
T52
New Zealand, 2005, Fermenting grape juice, Goddard et al. 2010
-0.0265 (± 0.1869)
T63
New Zealand, 2005, Fermenting grape juice, Goddard et al. 2010
-0.0052 (± 0.1923)
T78
New Zealand, 2005, Fermenting grape juice, Goddard et al. 2010
-0.2222 (± 0.0720)
T8
New Zealand, 2005, Fermenting grape juice, Goddard et al. 2010
0.0759 (± 0.1441)
UATTSo1_A3
New Zealand, 2011, Native bush, soil, Goddard collection (unpublished)
-0.1548 (± 0.1824)
UMBMfrR_A10
New Zealand, 2011, Native bush, fruit, Goddard collection (unpublished)
0.0849 (± 0.1291)
UMMASoR_G9
New Zealand, 2011, Native bush, soil, Goddard collection (unpublished)
0.2515 (± 0.1605)
WA_C_CODDINGTON_2
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
0.0444 (± 0.1556)
WA_C_KINGSMILL_10
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
-0.0070 (± 0.1237)
WA_C_MATES_10
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
0.0501 (± 0.2169)
WA_C_MATES_13
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
0.0957 (± 0.0978)
WA_C_WAITAKEREROAD_7
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
0.2245 (± 0.0715)
WI_C_MB95MBMZ_4
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
-0.0146 (± 0.1091)
WI_C_MBSP_15
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
0.0103 (± 0.1402)
WI_C_MBSP_4
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
-0.0248 (± 0.1707)
WI_S_JASA_13
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
0.3141 (± 0.2245)
WI_S_JASA_5
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
-0.1183 (± 0.1099)
WI_S_OAKURA_4
New Zealand, 2010, Fermenting grape juice, Gayevskiy & Goddard 2012
-0.1948 (± 0.2694)
WSERCsf_G4
New Zealand, 2011, Fermenting grape juice, Goddard collection (unpublished)
-0.0387 (± 0.1871)
WSETAwf_B1
New Zealand, 2011, Fermenting grape juice, Goddard collection (unpublished)
0.0521 (± 0.1358)
273614N
United Kingdom, N/A, Clinical isolate (Fecal), Liti et al. 2009
0.2212 (± 0.0905)
378604X
United Kingdom, N/A, Clinical isolate (Sputum), Liti et al. 2009
0.0031 (± 0.1498)
BC187
USA, N/A, Barrel fermentation, Liti et al. 2009
0.2817 (± 0.0527)
DBVPG1106
Australia, 1947, Grapes, Liti et al. 2009
0.2760 (± 0.0964)
DBVPG1373
Netherlands, 1952, Soil, Liti et al. 2009
-0.0117 (± 0.0721)
DBVPG1788
Finland, 1957, Soil, Liti et al. 2009
0.2662 (± 0.1090)
DBVPG1853
Ethipia, 1959, White teff, Liti et al. 2009
0.0075 (± 0.1628)
DBVPG6040
Netherlands, 1952, Fermenting fruit juice, Liti et al. 2009
-0.0690 (± 0.2116)
DBVPG6044
West Africa, 1925, Bili wine (Osbeckia grandiflora), Liti et al. 2009
0.1047 (± 0.0675)
DBVPG6765
N/A, N/A, N/A, Liti et al. 2009
0.0417 (± 0.2096)
K11
Japan, 1981, Shochu sake strain, Liti et al. 2009
0.1636 (± 0.1411)
L-1374
Chile, 1999, Juice, Liti et al. 2009
-0.0279 (± 0.1343)
L-1528
Chile, 1999, Juice, Liti et al. 2009
0.0188 (± 0.1751)
NCYC110
West Africa, pre1914, Ginger beer, Liti et al. 2009
-0.0675 (± 0.1253)
NCYC361
Ireland, 1952, Beer, Liti et al. 2009
0.1300 (± 0.2580)
RM11_1A
N/A, N/A, N/A, Liti et al. 2009
-0.1591 (± 0.2045)
S288c
USA, 1938, Rotting fig, Liti et al. 2009
-0.1294 (± 0.1642)
SK1
USA, pre1974, Soil, Liti et al. 2009
-0.0975 (± 0.1984)
UWOPS03.461.4
Malaysia, 2003, Nectar, Bertram palm, Liti et al. 2009
-0.0488 (± 0.2211)
UWOPS05.217.3
Malaysia, 2005, Nectar, Bertram palm, Liti et al. 2009
-0.1307 (± 0.1027)
UWOPS05.227.2
Malaysia, 2005, Trigona spp, Liti et al. 2009
0.1244 (± 0.0873)
UWOPS83.787.3
Bahamas, 1983, Fruit (Opuntia stricta), Liti et al. 2009
0.0116 (± 0.0730)
W303
created by rothstein, N/A, N/A, Liti et al. 2009
0.2108 (± 0.0483)
Y12
Ivory Coast, pre1981, Palm wine, Liti et al. 2009
0.2217 (± 0.1122)
Y55
France, 1930, Grapes, Liti et al. 2009
0.1586 (± 0.1996)
Y9
Indonesia, pre1962, Ragi, Liti et al. 2009
0.1226 (± 0.1649)
YIIc17-E5
France, N/A, Wine, Liti et al. 2009
0.1541 (± 0.1209)
YJM145
N/A, N/A, N/A, Liti et al. 2009
-0.0655 (± 0.0506)
YJM975
Italy, 1994, Human, Liti et al. 2009
0.1819 (± 0.1103)
YJM978
Italy, 1994, Human, Liti et al. 2009
-0.2028 (± 0.1414)
YJM981
Italy, 1994, Human, Liti et al. 2009
0.1584 (± 0.3039)
YPS128
USA, 1999, Soil, Liti et al. 2009
0.4147 (± 0.0349)
YPS606
USA, 1999, Bark, Liti et al. 2009
0.2845 (± 0.0365)
2
YS2
Astralia, N/A, Baker strain, Liti et al. 2009
0.2903 (± 0.0394)
YS4
Netherlands, 1975, Baker strain, Liti et al. 2009
0.3801 (± 0.0395)
YS9
Singapore, N/A, Baker strain, Liti et al. 2009
0.4823 (± 0.0590)
EC1118
France, N/A, N/A, commercial wine yeast
-0.0771 (± 0.2330)
F15
France, N/A, N/A, commercial wine yeast
0.1067 (± 0.2696)
Lalvin 43
France, N/A, N/A, commercial wine yeast
0.0452 (± 0.1445)
VL3
France, N/A, N/A, commercial wine yeast
-0.3037 (± 0.0245)
X5
France , N/A, N/A, commercial wine yeast
0.2193 (± 0.0784)
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Table S2: D. simulans egg distribution in the laboratory cage experiment (n = 6)
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Number of eggs (mean ± SD) found on the source plate and the sum and average of all capture
17
plates for the three different yeast treatments.
Attractive yeast
Unattractive yeast
No yeast (control)
Source plate
146.27 ± 27.66
87.00 ± 35.52
103.55 ± 30.05
Capture plates (sum)
299.00 ± 51.70
273.16 ± 76.48
389.50 ± 206.95
Capture plate (average)
16.46 ± 12.90
14.9 ± 13.86
21.24 ± 19.85
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3