Supplemental Table 1. Listing of the tests used for the viral testing.
Cell line “I6”

Mouse antibody production test with LCMV challenge

Evaluation by real-time quantitative PCR of the presence of human
immunodeficiency virus type 1 (HIV-1) RNA sequences

Evaluation by real-time quantitative PCR of the presence of human
immunodeficiency virus type 2 (HIV-2) RNA sequences

Evaluation by real-time quantitative PCR of the presence of human hepatitis C
virus (HCV) RNA sequences

Evaluation by real-time quantitative PCR of the presence of human
Enterovirus RNA sequences

Evaluation by real-time quantitative PCR of the presence of Human T
Leukemia Virus type I and II (HTLV-1 and 2) DNA sequences

Evaluation by real-time quantitative PCR of the presence of human hepatitis B
virus (HBV) DNA sequences

Evaluation by real-time quantitative PCR of the presence of human EpsteinBarr Virus (EBV) DNA sequences

Evaluation by real-time quantitative PCR of the presence of Human
Cytomegalovirus (HCMV) DNA sequences

Evaluation by real-time quantitative PCR of the presence of human herpes
virus 6 (HHV6) DNA sequences

Evaluation by real-time quantitative PCR of the presence of human herpes
virus 7 (HHV7) DNA sequences

Evaluation by real-time quantitative PCR of the presence of human
Papillomavirus DNA sequences using Taqman technology

Evaluation by real-time quantitative PCR of the presence of human
Polyomavirus JC and BK DNA sequences

Evaluation by real-time quantitative PCR of the presence of human herpes
virus 8 (HHV8) DNA sequences

Detection of viral contaminants using porcine cells (PPK cells)

In vitro assay for the detection of bovine viruses according to 9CFR
Supernatant from I6 cell culture
Cultivable and non cultivable mycoplasma detection according to European
Pharmacopeia
Master/Working Cell bank (P38)

Isoenzyme Analysis

Transmission electron microscopy for detection of adventitious agents into
cells (Stade I)

Evaluation by F-PERT of the presence of reverse transcriptase activity

Evaluation by polymerase chain reaction of the presence of human AdenoAssociated Viruses (AAV) DNA sequences

Evaluation by real-time quantitative PCR of the presence of human
Erythrovirus B19 DNA sequences

Evaluation by real-time quantitative PCR of the presence of human hepatitis A
virus (HAV) RNA sequences

Determination of viral contaminants using MRC-5 and Vero cell lines

Detection of viral contaminants using human cells (HEK293)

Test for basteriostatic and fungistatic activity using a direct inoculation
method (2 media)

Detection of bacteria and fungi contaminants using a direct inoculation
method (2 media)

Cultivable and non cultivable mycoplasma detection according to European
Pharmacopeia

Detection of viral contaminants using suckling and adult CD-1 mice according
to European Pharmacopeia

Detection of viral contaminants in virus seed lot using hen embryonated eggs
according to European Pharmacopoeia

Detection of viral contaminants using porcine cells (PPK cells)

In vitro assay for the detection of bovine viruses according to 9CFR

Supra/extended assay for murine Xenotropic/Amphotropic/MCF Retrovirus
detection
Late Production Cell bank (P44)

Detection of viral contaminants using suckling and adult CD-1 mice according
to European Pharmacopeia

Detection of viral contaminants in virus seed lot using hen embryonated eggs
according to European Pharmacopoeia

Detection of viral contaminants using porcine cells (PPK cells)

In vitro assay for the detection of bovine viruses according to 9CFR
SSEA-1+ progenitors (P41)

Cultivable and non cultivable mycoplasma detection according to European
Pharmacopeia

Determination of viral contaminants using MRC-5 and Vero cell lines

Detection of viral contaminants using human cells (HEK293)

Evaluation by F-PERT of the presence of reverse transcriptase activity

In vitro assay for the detection of bovine viruses according to 9CFR
SSEA-1+ progenitors (P47)

Cultivable and non cultivable mycoplasma detection according to European
Pharmacopeia

Determination of viral contaminants using MRC-5 and Vero cell lines

Detection of viral contaminants using human cells (HEK293)

Evaluation by F-PERT of the presence of reverse transcriptase activity

In vitro assay for the detection of bovine viruses according to 9CFR
Anti-SSEA-1 antibody : Lysate of cells, clone VIM C6 Batch 5110705246

Extended assay for murine Xenotropic/Amphotropic/MCF Retrovirus
detection

Detection of viral contaminants using mouse cells (NIH/3T3)

Determination of viral contaminants using MRC-5 and Vero cell lines

Transmission electron microscopy for detection of adventitious agents into
cells (Stade I)
Anti-SSEA-1 antibody : 530 Anti-SSEA 1 (CD15)-Microbeads batch 5110824021

Detection of viral contaminants using mouse cells (NIH/3T3)

Determination of viral contaminants using MRC-5 and Vero cell lines

Analysis by negative stain electron microscopy